Abstract
The tobacco BY-2 cell line was established from a callus induced on a seedling of Nicotiana tabacum L. cv. Bright Yellow 2 (Kato et al. 1972). Because of its unique characteristics such as an exceptionally high growth rate and high homogeneity, the BY-2 cell line has now become one of the most popular model systems for studies of cell biology and biochemistry in higher plants (Nagata et al. 1992). Besides being used as an ideal model of plant cells, BY-2 cells have often been regarded as relatively featureless cells that can only grow rapidly without remarkable differentiation of organelles. In spite of this impression, BY-2 cells have already been used in many aspects of plant organelle research. Recent studies, moreover, have revealed that the organelles in BY-2 cells exhibit dynamic changes in morphology and function, depending on their growth phases and culture conditions. Such findings further extend the potential use of this cell line as a material for studying plant organelle dynamics.
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Sakai, A., Miyazawa, Y., Kuroiwa, T. (2004). Studies on Dynamic Changes of Organelles Using Tobacco BY-2 as the Model Plant Cell Line. In: Nagata, T., Hasezawa, S., Inzé, D. (eds) Tobacco BY-2 Cells. Biotechnology in Agriculture and Forestry, vol 53. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10572-6_14
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DOI: https://doi.org/10.1007/978-3-662-10572-6_14
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