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Block Points in the Cell Cycle Progression of Plant Cells: Deduced Lessons from Tobacco BY-2 Cells

  • Toshio Sano
  • Takashi Shimizu
  • Kenichi Sakamoto
  • Toshiyuki Nagata
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 53)

Abstract

The proliferation of cells is an essential framework for plant growth and development, as cells supplied by cell division constitute plant body; however, a lot of issues on this subject remain to be clarified. Although there are common features in the proliferation of eukaryotic cells, there are several unique characteristics that are inherent in plant cells. For instance, the septum formation at the completion of cytokinesis results in the alignment of daughter cells side by side which constitute plant bodies with a sessile nature. Although a cytological description of plant cell division has been done with a few cells in tissues such as root tips, comprehensive views particularly on molecular terms cannot be demonstrated with such microscope methods and can be done only with the use of highly synchronized cell populations. As noted by Nagata in the first chapter of this volume, this high synchrony was attained in 1982, some 20 years ago, when the cell cycle arrest at G1/S phase by aphidicolin, an inhibitor of DNA polymerase a, and the release of this drug allowed a synchronized cell population starting from S phase to be obtained (Nagata et al. 1982). In addition, a combination of aphidicolin treatment and subsequent propyzamide treatment, a microtubule destabilizing drug, brought us an even more highly synchronized system starting from M phase (Kakimoto and Shibaoka 1988, Nagata et al. 1992). Since then, the high cell synchrony methods using tobacco BY-2 cells are the only available ones for higher plants and even today, no alternative systems in higher plant cell lines have been reported. For this reason, this system is considered to be the inevitable one for studies of various aspects of plant cells and many other issues (Nagata et al. 1992), major themes of which are handled in other chapters of this volume.

Keywords

Cell Cycle Progression Phosphate Starvation Phosphate Addition Plant Cell Cycle Induce Cell Division 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Toshio Sano
    • 1
  • Takashi Shimizu
    • 1
  • Kenichi Sakamoto
    • 1
  • Toshiyuki Nagata
    • 1
  1. 1.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoBunkyo-ku, TokyoJapan

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