When I Encountered Tobacco BY-2 Cells!

  • Toshiyuki Nagata
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 53)


The tobacco BY-2 cell line has attained a unique position in the plant science community. Although it is one of many plant cell lines, it has spread to at least 27 countries, as far as I am aware, and more than 500 papers in which the BY-2 cell line is handled as experimental material have been published from 1990–1999. Since then, publications on this cell line are steadily increasing. It seems that this cell line has been brought to much wider locations than the above-mentioned 27 countries as well without my notice. Thus, the basic knowledge on the molecular and cellular biology of plant cells is most extensively and intensively accumulated on this cell line (Nagata et al. 1992; Geelen and Inzé 2001). This is one of the strong reasons why this volume is included in this series of Biotechnology in Agriculture and Forestry (BAF). The accumulated knowledge can be shared with scientists of applied as well as basic fields. In this situation, it is worthwhile to reflect what happened in the earlier stage of the culture of tobacco BY-2 cells.


Tobacco Mosaic Virus Plant Protoplast High Synchrony Cell Synchrony Cell Cycle Synchrony 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Asada T, Sonobe S, Shibaoka T (1991) Microtubule translocation in the cytokinetic apparatus of cultured tobacco cells. Nature 350: 238–241CrossRefGoogle Scholar
  2. Fukunaga Y, Nagata T, Takebe I (1981) Liposome-mediated infection of plant protoplasts with tobacco mosaic virus RNA. Virology 113: 752–760PubMedCrossRefGoogle Scholar
  3. Geelen DNV, Inzé D (2001) A bright future of the Bright Yellow-2 cell culture. Plant Physiol 127; 1375–1379PubMedCrossRefGoogle Scholar
  4. Kakimoto T, Shibaoka T (1988) Cytoskeletal ultrastructure of phragmoplast-nuclei complexes isolated from cultured tobacco cells. Protoplasma Suppl 2: 95–103CrossRefGoogle Scholar
  5. Kato K, Matsumoto T, Koiwai A, Mizusaki S, Nishida K, Noguchi M, Tamaki E (1972) Liquid suspension culture of tobacco cells. Ferment Technol Today: 689–695Google Scholar
  6. Kikkawa H, Nagata T, Matsui C, Takebe I (1982) Infection of tobacco suspension cultures by tobacco mosaic virus. J Gen Virol 63: 451–456CrossRefGoogle Scholar
  7. Nagata T (1984) Interaction of liposomes and protoplasts as a model system of protoplast fusion. In: Beers RF Jr, Bassett EG (eds) Cell fusion: gene transfer and transformation. Raven Press, New York, pp. 217–226Google Scholar
  8. Nagata T (1989) Cell biological aspects of gene delivery into plant protoplasts by electroporation. Int Rev Cytol 116: 229–255CrossRefGoogle Scholar
  9. Nagata T, Kumagai F (1999) Plant cell biology through the window of the highly synchronized tobacco BY-2 cell line. Method Cell Sci 21: 123–127CrossRefGoogle Scholar
  10. Nagata T, Okada K, Takebe I, Matsui C (1981) Delivery of tobacco mosaic virus RNA into protoplasts mediated by reverse-evaporation vesicles (liposomes). Mol Gen Genet 184:161– 165Google Scholar
  11. Nagata T, Oakada K, Takebe I (1982) Mitotic protoplasts and their interaction with tobacco mosaic virus RNA encapsulated in liposomes. Plant Cell Rep 1: 250–252CrossRefGoogle Scholar
  12. Nagata T, Okada K, Kawazu T, Takebe I (1987) Cauliflower mosaic virus 35S RNA promoter directs S phase specific gene expression in plant cells. Mol Gen Genet 207: 242–244CrossRefGoogle Scholar
  13. Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cell line as the “HeLâ cells in the cell biology of higher plants. Int Rev Cytol 132: 1–30CrossRefGoogle Scholar
  14. Okada K, Takebe I, Nagata T (1986) Expression and integration of genes into highly synchronized plant protoplasts. Mol Gen Genet 205: 398–403CrossRefGoogle Scholar
  15. Pedrali-Noy G, Spadari S, Miller-Faures A, Miller AO, Kruppa J, Koch G (1980) Synchronization of He La cell cultures by inhibition of DNA polymerase a with aphidicolin. Biochem Biophys Res Commun 8: 377–387Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Toshiyuki Nagata
    • 1
  1. 1.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoBunkyo-ku, TokyoJapan

Personalised recommendations