Nitrogen-Fixing Enterobacter: Structure of nif-Gene Group, nif-Plasmid Spread and Risk Assessment for Releases

  • Walter Klingmüller

Abstract

To provide basic knowledge for their exploitation in association with cereals, nitrogen-fixing Enterobacter agglomerans strains from the rhizosphere of wheat were studied from two view points: 1) The molecular basis of nitrogen fixation, and 2) The risk of gene spread upon release.

Keywords

Sucrose Maize Bacillus Photosynthesis Pseudomonas 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abdel-Salam MS, Klingmüller W (1987) Transposon Tn5 mutagencsis in Azospirillum lipo/erum: isolation of indole acetic acid mutants. Mol Gen Genet 210: 165CrossRefGoogle Scholar
  2. Boyer HW, Rouillard-Dussioux D (1969) Complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41: 459CrossRefGoogle Scholar
  3. Casida LE Jr (1989) Protozoan response to the addition of bacterial predators and other bacteria to soil. Appl Envir Microbiol 55: 1857Google Scholar
  4. Döhler K, Klingmüller W (1988) Genetic interaction of Rhizobium leguminosarum biovar riceae with Gram-negative bacteria. In: Klingmüller W (ed) Risk assessment for deliberate releases: The possible impact of genetechnically engineered microorganisms on the environment Springer-Verlag Berlin-Heidelberg New York Tokyo, p 18Google Scholar
  5. van Elsas JD, Govaert JM, van Veen JA (1987) Transfer of plasmid pET-30 between bacilli in soil as influenced by bacterial population dynamics and soil conditions. Soil Biol Biochem 19: 639CrossRefGoogle Scholar
  6. van Elsas JD, Trevors JT, Starodub ME (1988a) Plasmid transfer in soil and rhizosphere In: Klingmüller W (ed) Risk assessment for deliberate releases Springer-Verlag Berlin-Heidlberg New York Tokyo, p 89Google Scholar
  7. van Elsas JD, Trevors JT, Starodub ME (1988b) Bacterial conjugation between pseudomonads in the rhizosphere of wheat. FEMS Microbiol Ecol 53: 299CrossRefGoogle Scholar
  8. Haahtela K (1985) Nitrogenase activity (acetylene reduction) in root-associated, cold-climate species of Azospirillum, Enterobacter, Kleb.siella and Pseudo,nonas, growing at various temperatures. FEMS Microbiol Ecol 31: 211CrossRefGoogle Scholar
  9. Herterich S (1986) Untersuchung der Transfereigenschaft des Plasmids pEA9 aus Enterobacter agglomerans 339 and Entwicklung eines Transposon-Mutagenesesystems. Diplom-thesis, University of BayreuthGoogle Scholar
  10. Jagnow G (1984) Stickstoffbindende Bakterien in der Rhzzosphäre I. Bindungsraten im Freiland, Artenvielfalt and Verbreitung. Kali-Briefe (Büntehof) 17: 341Google Scholar
  11. Jagnow G (1988) Enterobacteriaceae in the rhizosphere of wheat, barley and ryegrass: Fractions of nitrogenase-positive strains in field experiments with different doses of nitrogen fertilizer. In: Bothe H, de Brujin FJ, Newton WE (eds) Nitrogen fixation: hundred years after. Gustav Fischer Stuttgart-New YorkGoogle Scholar
  12. Kado CI, Liu ST (1981) Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 145: 1365Google Scholar
  13. Keen NT, Tamaki S, Kobayashi D, Trollinger D (1988) Improved broad-host range plasmids for DNA cloning in Gram-negative bacteria. Gene 70: 191CrossRefGoogle Scholar
  14. Kleeberger A, Castorph H, Klingmüller W (1983) The rhizosphere microflora of wheat and barley with special reference to Gram-negative bacteria. Arch Microbiol 136: 306CrossRefGoogle Scholar
  15. Klingmüller W (1985) Studies on natural and artificial nitrogen-fixing soil bacteria. In: Sinha UGoogle Scholar
  16. Klingmüller W (eds) Trends in molecular genetics. Spectrum Publ House. Patna, New Delhi, p 37 Klingmüller W (ed) (1988a) Azospirillum IV: genetics, physiology, ecology. Springer-Verlag, Berlin Heidelberg New York TokyoGoogle Scholar
  17. Klingmüller W (1988b) Molecular analysis of nitrogen fixation in Enterobacter. Proc Int Symp on Plant Biotech. Gyeongsang Natl Univ Chinju, Korea, p 41Google Scholar
  18. Klingmüller W (1991) Plasmid transfer in natural soil: Assessing nitrogen fixing Emerobactcr for safe release. FEMS Microbiol [col 85: 107CrossRefGoogle Scholar
  19. Klingmüller W, Herterich S, Min BW (1989) Self-transmissible nif-plasmids in Enterohacter. In: Skinner FA, Boddey RM, Fendrik I (eds) Nitrogen fixation with non-legumes. Kluwer, Dordrecht, p 173CrossRefGoogle Scholar
  20. Klingmüller W, Daily A, Fentner C, Steinlein M (1990) Plasmid transfer between soil bacteria. In: Fry JC, Day MJ (eds) Bacterial genetics in natural environments. Chapman and Hall, London, p 133CrossRefGoogle Scholar
  21. Krasovsky VN, Stotzky G (1987) Conjugation and genetic recombination in Escherichia coli in sterile and non-sterile soil. Soil Biol Biochem 19: 631CrossRefGoogle Scholar
  22. Kreutzer R (1986) Restriktionskartierung eines nif-plasmids von Enterohacter agglomerans und genetische Charakterisierung der von ihm getragenen nil-Gene. Diplom-thesis, University of BayreuthGoogle Scholar
  23. Kreutzer R, Singh M, Klingmüller W (1989) Identification and characterization of the OH and nifJ promoter regions located on the nif-plasmid pEA3 of Enterobacter agglomerans 333. Gene 78: 101CrossRefGoogle Scholar
  24. Kreutzer R, Steibl HD, Dayananda S, Dippe R, Halda L, Buck M, Klingmüller W (1991) Genetic characterization of nitrogen fixation in Enterohacter strains from the rhizosphere of cereals. In: Polsinelli M, Materassi R, Vinzencini M (eds) Nitrogen fixation. Kluwer, Dordrecht, p 25CrossRefGoogle Scholar
  25. Lindberg T, Granhall U (1984) Isolation and characterization of dinitrogen-fixing bacteria from the rhizosphere of temperate cereals and forage grasses. Appl Envir Microbiol 48: 683Google Scholar
  26. Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.Google Scholar
  27. Min BW (1986) Erzeugung von nif--Mutanten des Stammes Enterobacter agglomerans 339 durch Transposonmutagenese und Untersuchung der Transfereigenschaft seines Plasmids pEA9. Diplom-thesis, University of BayreuthGoogle Scholar
  28. Polsinelli M, Materassi R, Vincenzini M (1991) Nitrogen fixation, Kluwer, DordrechtCrossRefGoogle Scholar
  29. Riedel GE, Brown SE, Ausubel FM (1983) Nitrogen fixation by Klebsiella pneumoniae is inhibited by certain multicopy hybrid nif plasmids. J Bacteriol 153: 45Google Scholar
  30. Schilf W, Klingmüller W (1983) Experiments with Escherichia coli on the dispersal of plasmids in environmental samples. Recombinant DNA Technical Bulletin, Washington, 6 (3): 101Google Scholar
  31. Simon R (1984) High frequency mobilization of Gram-negative bacterial replicons by the in vitro constructed Tn5-mob transposon. Mol Gen Genet 196: 413CrossRefGoogle Scholar
  32. Singh M, Klingmüller W (1985) Nif-plasmids in free-living nitrogen fixing soil bacteria. In: Sinha U, Klingmüller W (eds) Trends in molecular genetics. Spectrum Publ. House, Patna New Delhi, p15Google Scholar
  33. Singh M, Klingmüller W (1986a) Transposon mutagenesis in Azospirillum brasilense: isolation of auxotrophic and nif-mutants and molecular cloning of the mutagenized nifDNA. Mol Gen Genet 202: 136CrossRefGoogle Scholar
  34. Singh M, Klingmüller W (1986b) Cloning of pEA3, a large plasmid of Enterohacter agglomerans containing nitrogenase structural genes. Plant and Soil 90: 235CrossRefGoogle Scholar
  35. Singh M, Kleeberger A, Klingmüller W (1983) Location of nitrogen fixation (nil) genes on indigenous plasmids of Enterohacter agglomerans. Mol Gen Genet 190: 373CrossRefGoogle Scholar
  36. Singh M, Kreutzer R, Acker G, Klingmüller W (1988) Localization and physical mapping of a plasmid-borne 23 kb nif-gene cluster from Enterohacter agglomerans showing homology to the entire nif-gene cluster of Klebsiella pneumoniae MSal. Plasmid 19: IGoogle Scholar
  37. Skinner FA, Boddey RM, Fendrik I (1989) Nitrogen fixation with non-legumes. Kluwer, Dordrecht Steibl HD (1989) Klonierung des Plasmids pEA9 von Enterohacter agglomerans 339 und Analyse der plasmidalen nif-Genorganisation. Diplom-thesis, University of BayreuthGoogle Scholar
  38. Trevors JT, Starodub ME (1987) R-plasmid transfer in non-sterile agricultural soil. System Appl Microbiol 9: 312CrossRefGoogle Scholar
  39. Trevors JT, Barkar T, Bourquin AW (1987) Genetransfer among bacteria in soil and aquatic environments: A Review. Can J Microbiol 33: 191Google Scholar
  40. Väisänen O, Haahtela K, Bask L, Kari K, Salkinoja-Salonen M, Sundman V (1985) Diversity of nif gene location and nitrogen fixation among root-associated Enterohacter and Klebsiella strains. Arch Microbiol 141: 123CrossRefGoogle Scholar
  41. Wang PL, Koh SK, Chung KS, Uozumi T, Beppu T (1985) Cloning and Expression in E. coli of the whole nif genes of Klebsiella oxytoca, a nitrogen fixer in the rhizosphere of rice. Agric Biol Chem 49 (5): 1469CrossRefGoogle Scholar
  42. Zhu JB, Li ZG, Wang.LW, Shen SS, Shen SC (1986) Temperature sensitivity of a nifA-like gene in Enterohacter cloacae. J Bacteriology 166: 35Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Walter Klingmüller

There are no affiliations available

Personalised recommendations