Multiple Binding Sites in the Complex Between the nodA-D Intergenic Region the Product of the nodD Gene

  • G. F. Hong
  • H. M. Cao
  • F. D. Ni
  • Y. Y. Lu


The following findings have been made:
  1. (1)

    NodD (nodD gene product) specifically binds to the nodA-D DNA, which spans the intergenic region of nodD and nodA genes (Hong et al. 1987). (The nodA-D DNA sequence is available at the end of this paper).

  2. (2)

    NodD binds to the nodA-D DNA, resulting in as many as seven complexes, which have different velocities when they are subject to electrophoresis on the native polyacrylamide gel.

  3. (3)

    Naringenin—a plant-produced signal molecule—destabilizes the complexes formed between NodD and the nodA-D DNA.



Rhizobium Leguminosarum Symbiotic Plasmid Retarded Band Multiple Binding Site Offering Strain 
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  4. Rossen L et al. (1985) EMBO J 4: 3369Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • G. F. Hong
  • H. M. Cao
  • F. D. Ni
  • Y. Y. Lu

There are no affiliations available

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