Multiple Binding Sites in the Complex Between the nodA-D Intergenic Region the Product of the nodD Gene
NodD (nodD gene product) specifically binds to the nodA-D DNA, which spans the intergenic region of nodD and nodA genes (Hong et al. 1987). (The nodA-D DNA sequence is available at the end of this paper).
NodD binds to the nodA-D DNA, resulting in as many as seven complexes, which have different velocities when they are subject to electrophoresis on the native polyacrylamide gel.
Naringenin—a plant-produced signal molecule—destabilizes the complexes formed between NodD and the nodA-D DNA.
KeywordsElectrophoresis Polyacrylamide Flavonoid Rhizobium Naringenin
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