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Multiple Binding Sites in the Complex Between the nodA-D Intergenic Region the Product of the nodD Gene

  • G. F. Hong
  • H. M. Cao
  • F. D. Ni
  • Y. Y. Lu

Abstract

The following findings have been made:
  1. (1)

    NodD (nodD gene product) specifically binds to the nodA-D DNA, which spans the intergenic region of nodD and nodA genes (Hong et al. 1987). (The nodA-D DNA sequence is available at the end of this paper).

     
  2. (2)

    NodD binds to the nodA-D DNA, resulting in as many as seven complexes, which have different velocities when they are subject to electrophoresis on the native polyacrylamide gel.

     
  3. (3)

    Naringenin—a plant-produced signal molecule—destabilizes the complexes formed between NodD and the nodA-D DNA.

     

Keywords

Rhizobium Leguminosarum Symbiotic Plasmid Retarded Band Multiple Binding Site Offering Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bagdasarian M et al. (1981) Gene 16: 237CrossRefGoogle Scholar
  2. Firmin JL et al. (1986) Nature 324: 90CrossRefGoogle Scholar
  3. Hen-ming Wu, Crothers DM (1984) Nature 308: 509 Hong GF. (1987) Nucl Acid Res 15: 9677 Lamb JW. (1985) Gene 34: 235Google Scholar
  4. Rossen L et al. (1985) EMBO J 4: 3369Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • G. F. Hong
  • H. M. Cao
  • F. D. Ni
  • Y. Y. Lu

There are no affiliations available

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