Multiple Binding Sites in the Complex Between the nodA-D Intergenic Region the Product of the nodD Gene

  • G. F. Hong
  • H. M. Cao
  • F. D. Ni
  • Y. Y. Lu

Abstract

The following findings have been made:
  1. (1)

    NodD (nodD gene product) specifically binds to the nodA-D DNA, which spans the intergenic region of nodD and nodA genes (Hong et al. 1987). (The nodA-D DNA sequence is available at the end of this paper).

     
  2. (2)

    NodD binds to the nodA-D DNA, resulting in as many as seven complexes, which have different velocities when they are subject to electrophoresis on the native polyacrylamide gel.

     
  3. (3)

    Naringenin—a plant-produced signal molecule—destabilizes the complexes formed between NodD and the nodA-D DNA.

     

Keywords

Electrophoresis Polyacrylamide Flavonoid Rhizobium Naringenin 

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References

  1. Bagdasarian M et al. (1981) Gene 16: 237CrossRefGoogle Scholar
  2. Firmin JL et al. (1986) Nature 324: 90CrossRefGoogle Scholar
  3. Hen-ming Wu, Crothers DM (1984) Nature 308: 509 Hong GF. (1987) Nucl Acid Res 15: 9677 Lamb JW. (1985) Gene 34: 235Google Scholar
  4. Rossen L et al. (1985) EMBO J 4: 3369Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • G. F. Hong
  • H. M. Cao
  • F. D. Ni
  • Y. Y. Lu

There are no affiliations available

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