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Advances in Diagnostic Methods for Invasive Candida and Aspergillus Infections

  • Chapter
Human Fungal Pathogens

Part of the book series: The Mycota ((MYCOTA,volume 12))

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Abstract

There is a growing incidence of human disease due to opportunistic fungal infections. The population of immunocompromised, neutropenic, or critically ill patients at risk for invasive mycoses is a consequence of increasingly successful, but complex therapies employed by modern medicine. The major opportunistic fungal pathogens responsible for these infections include Candida species, Aspergillus species and other opportunistic molds, and Cryptococcus neoformans. Standard clinical examination, diagnostic imaging methods, and conventional microbiological methods are of limited utility in timely diagnosis of invasive mycoses. For cryptococcosis, sensitive and specific antigen detection tests are routinely used to diagnose this infection. In contrast, the diagnosis of invasive candidiasis and aspergillosis remains problematic. Consequently, substantial efforts have been undertaken to investigate novel methods for the diagnosis of these opportunistic mycoses.

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References

  • Aisner J, Schimpff SC, Wiernik PH (1977) Treatment of invasive aspergillosis: relation of early diagnosis and treatment to response. Ann Intern Med 86:539–543

    PubMed  CAS  Google Scholar 

  • Andrews CP, Weiner MH (1981) Immunodiagnosis of invasive pulmonary aspergillosis in rabbits. Fungal antigens detected by radioimmunoassay in bronchoalveolar lavage fluid. Am Rev Respir Dis 124:60–64

    PubMed  CAS  Google Scholar 

  • Andriole VT (1993) Infections with Aspergillus species. Clin Infect Dis 17 [Suppl 2]:S481–S486

    PubMed  Google Scholar 

  • Angiolella L, Facchin M, Stringaro A, Maras B, Simonetti N, Cassone A (1996) Identification of a glucan-associated enolase as a main cell wall protein of Candida albicans and an indirect target of lipopeptide antimycotics. J Infect Dis 173:684–690

    PubMed  CAS  Google Scholar 

  • Ansorg R, Heintschel von Heinegg E, Rath PM (1994) Aspergillus antigenuria compared to antigenemia in bone marrow transplant recipients. Eur J Clin Microbiol Infect Dis 13:582–589

    Google Scholar 

  • Bailey JW, Sada E, Brass C, Bennett JE (1985) Diagnosis of systemic candidiasis by latex agglutination for serum antigen. J Clin Microbiol 21:749–752

    PubMed  CAS  Google Scholar 

  • Banerjee SN, Emori G, Culver DH, Gaynes RP, Jarvis WR, Horan T, Edwards JR, Tolson J, Henderson T, Martone WJ (1991) Secular trends in nosocomial primary bloodstream infections in the United States. Am J Med 91 [Suppl 3B]:86–89

    Google Scholar 

  • Becker MJ, de Marie S, Willemse D, Verbrugh HA, Bakker-Woudenberg IA (2000) Quantitative galactomannan detection is superior to PCR in diagnosing and monitoring invasive pulmonary aspergillosis in an experimental rat model. J Clin Microbiol 38:1434–1438

    PubMed  CAS  Google Scholar 

  • Beck-Sague CM, Jarvis WR (1993) Secular trends in the epidemiology of nosocomial fungal infections in the United States. J Infect Dis 167:1247–1251

    PubMed  CAS  Google Scholar 

  • Bennett JE (1987) Rapid diagnosis of candidiasis and aspergillosis. Rev Infect Dis 9:398–402

    PubMed  CAS  Google Scholar 

  • Berenguer J, Buck M, Witebsky F, Stock F, Pizzo PA, Walsh TJ (1993) Lysis-centrifugation blood cultures in the detection of tissue-proven invasive candidiasis: disseminated versus single organ infection. Diagn Microbiol Infect Dis 17:103–109

    PubMed  CAS  Google Scholar 

  • Bernard EM, Christiansen KJ, Tsang SF, Kiehn TE, Armstrong D (1981) Rate of arabinitol production by pathogenic yeast species. J Clin Microbiol 14:189–194

    PubMed  CAS  Google Scholar 

  • Bougnoux ME, Hill C, Moissenet D, De Chauvin MF, Bonnay M, Vicens-Sprauel I, Pietri F, McNeil M, Kaufman L, Dupouy-Camet (1990) Comparison of antibody, antigen, and metabolite assays for hospitalized patients with disseminated or peripheral candidiasis. J Clin Microbiol 28:905–909

    PubMed  CAS  Google Scholar 

  • Bougnoux ME, Dupont C, Mateo J, Saulnier P, Faivre V, Payen D, Nicolas-Chanoine M-H (1999) Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR. J Clin Microbiol 37:925–930

    PubMed  CAS  Google Scholar 

  • Brandt ME, Padhye AA, Mayer LW, Holloway BP (1998) Utility of random amplified polymorphic DNA PCR and TaqMan automated detection in molecular identification of Aspergillus fumigatus. J Clin Microbiol 36:2057–2062

    PubMed  CAS  Google Scholar 

  • Bretagne S, Costa JM, Marmorat-Khuong A, Poron F, Cordonnier C, Vidaud M, Fleury-Feith J (1995) Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR. J Clin Microbiol 33:1164–1168

    PubMed  CAS  Google Scholar 

  • Bretagne S, Marmorat-Khuong A, Kuentz M, Latge JP, Bart-Delabesse E, Cordonnier C (1997) Serum Aspergillus galactomannan antigen testing by sandwich ELISA: practical used in neutropenic patients. J Infect 35:7–15

    PubMed  CAS  Google Scholar 

  • Bretagne S, Costa JM, Bart-Delabesse E, Dhedin N, Rieux C, Cordonnier C (1998) Comparison of serum galactomannan antigen detection and competitive polymerase chain reaction for diagnosing invasive aspergillosis. Clin Infect Dis 26:1407–1412

    PubMed  CAS  Google Scholar 

  • Burgener-Kairuz P, Zuber JP, Jaunin P, Buchman TG, Bille J, Rossier M (1994) Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment. J Clin Microbiol 32:1902–1907

    PubMed  CAS  Google Scholar 

  • Burnie JP (1995) Allergic and invasive aspergillosis. J R Soc Med 88 [Suppl 25]:41–45

    PubMed  Google Scholar 

  • Burnie JP, Williams JD (1985) Evaluation of the Ramco latex agglutination test in the early diagnosis of systemic candidiasis. Eur J Clin Microbiol 4:98–101

    PubMed  CAS  Google Scholar 

  • Burnie JP, Golbang N, Matthews RC (1997) Semiquantitative polymerase chain reaction enzyme immunoassay for diagnosis of disseminated candidiasis. Eur J Clin Microbiol Infect Dis 16:346–350

    PubMed  CAS  Google Scholar 

  • Cheung LL, Hudson JB (1988) Development of DNA probes for Candida albicans. Diagn Microbiol Infect Dis 10: 171–179

    PubMed  CAS  Google Scholar 

  • Christensson B, Roboz J (1991) Arabinitol enantiomers in cerebrospinal fluid. J Neurol Sci 105:234–239

    PubMed  CAS  Google Scholar 

  • Christensson B, Wiebe T, Pehrson C, Larsson L (1997) Diagnosis of invasive candidiasis in neutropenic children with cancer by determination of D-arabinitol/Larabinitol ratios in urine. J Clin Microbiol 35:636–640

    PubMed  CAS  Google Scholar 

  • Christensson B, Sigmundsdottir G, Larsson L (1999) Darabinitol — a marker for invasive candidiasis. Medical Mycol 37:391–396

    CAS  Google Scholar 

  • Chryssanthou E, Andersson B, Petrini B, Lofdahl S, Tollemar J (1994) Detection of Candida albicans DNA in serum by polymerase chain reaction. Scand J Infect Dis 26:479–485

    PubMed  CAS  Google Scholar 

  • Chryssanthou E, Klingspor L, Tollemar J, Petrini B, Larsson L, Christensson B, Ringden O (1999) PCR and other non-culture methods for diagnosis of invasive Candida infections in allogeneic bone marrow and solid organ transplant recipients. Mycoses 42:239–247

    PubMed  CAS  Google Scholar 

  • De Bernardis F, Girmenia C, Boccanera M, Adriani D, Martino P, Cassone A (1993) Use of a monoclonal antibody in a dot immunobinding assay for detection of a circulating mannoprotein of Candida spp. in neutropenic patients with invasive candidiasis. J Clin Microbiol 31:3142–3146

    PubMed  Google Scholar 

  • Denning DW (1996) Therapeutic outcome in invasive aspergillosis. Clin Infect Dis 23:608–615

    PubMed  CAS  Google Scholar 

  • De Repentigny L (1992) Serodiagnosis of candidiasis, aspergillosis, and cryptococcosis. Clin Infect Dis 14 [Suppl 1]:S11–S22

    PubMed  Google Scholar 

  • De Repentigny L, Marr L, Keller J, Carter A, Kuykendall R, Kaufman L, Reiss E (1985) Comparison of enzyme immunoassay and gas-liquid chromatography for the rapid diagnosis of invasive candidiasis in cancer patients. J Clin Microbiol 21:972–979

    PubMed  Google Scholar 

  • De Repentigny L, Kaufman L, Cole GT, Kruse D, Latge JP, Matthews RC (1994) Immunodiagnosis of invasive fungal infections. J Med Vet Mycol 32 [Suppl 1]: 239–252

    PubMed  Google Scholar 

  • Dupont B, Huber M, Kim SJ, Bennett JE (1987) Galactomannan antigenemia and antigenuria in aspergillosis: studies in patients and experimentally infected rabbits. J Infect Dis 155:1–11

    PubMed  CAS  Google Scholar 

  • Duthie R, Denning DW (1995) Aspergillus fungemia: report of two cases and review. Clin Infect Dis 20:598–605

    Google Scholar 

  • Einsele H, Hebart H, Roller G, Loffier J, Rothenhofer I, Muller CA, Bowden RA, van Burik J, Engelhard D, Kanz L, Schumacher U (1997) Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol 35:1353–1360

    PubMed  CAS  Google Scholar 

  • Eisen DP, Bartley PB, Hope W, Sigmundsdottir G, Pehrson C, Larsson L, Christensson B (2002) Urine D-arabinitol/ L-arabinitol ratio in diagnosing Candida infection in patients with haematological malignancy and HIV infection. Diagn Microbiol Infect Dis 42:39–42

    PubMed  CAS  Google Scholar 

  • Elie CM, Lott TJ, Reiss E, Morrison CJ (1998) Rapid identification of Candida species with species-specific DNA probes. J Clin Microbiol 36:3260–3265

    PubMed  CAS  Google Scholar 

  • Emori TG, Gaynes RP (1993) An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev 6:428–442

    PubMed  CAS  Google Scholar 

  • Escuro RS, Jacobs M, Gerson SL, Machicao AR, Lazarus HM (1989) Prospective evaluation of a Candida antigen detection test for invasive candidiasis in immunocompromised adult patients with cancer. Am J Med 87:621–627

    PubMed  CAS  Google Scholar 

  • Flahaut M, Sanglard D, Monod M, Bille J, Rossier M (1998) Rapid detection of Candida albicans in clinical samples by DNA amplification of common regions from C.albicans-secreted aspartic proteinase genes. Clin Microbiol 36:395–401

    CAS  Google Scholar 

  • Fletcher HA, Barton RC, Verweij PE, Evans EG (1998) Detection of Aspergillus fumigatus PCR products by a microtitre plate based DNA hybridisation assay. J Clin Pathol 51:617–620

    PubMed  CAS  Google Scholar 

  • Fortun J, Martin-Davila P, Alvarez ME, Sanchez-Sousa A, Quereda C, Navas E, Barcena R, Vicente E, Candelas A, Honrubia A, Nuno J, Pintado V, Moreno S, The Ramon y Cajal Hospital’s Liver Transplant Group (2001) Aspergillus antigenemia sandwich-enzyme immunoassay test as a serodiagnostic method for invasive aspergillosis in liver transplant recipients. Transplantation 71:145–149

    Google Scholar 

  • Francis P, Lee JW, Hoffman A, Peter J, Francesconi A, Bacher J, Shelhamer J, Pizzo PA, Walsh TJ (1994) Efficacy of unilamellar liposomal amphotericin B in treatment of pulmonary aspergillosis in persistently granulocytopenic rabbits: the potential role of bronchoalveolar D-mannitol and serum galactomannan as markers of infection. J Infect Dis 169:356–368

    PubMed  CAS  Google Scholar 

  • Franklyn KM, Warmington JR, Ott AK, Ashman RB (1990) An immunodominant antigen of Candida albicans shows homology to the enzyme enolase. Immunol Cell Biol 68:173–178

    PubMed  CAS  Google Scholar 

  • Fujita S, Hashimoto T (1992) Detection of serum Candida antigens by enzyme-linked immunosorbent assay and a latex agglutination test with anti-Candida albicans and anti-Candida krusei antibodies. J Clin Microbiol 30:3132–3137

    PubMed  CAS  Google Scholar 

  • Fujita S, Lasker BA, Lott TJ, Reiss E, Morrison CJ (1995) Microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species in blood. J Clin Microbiol 33:962–967

    PubMed  CAS  Google Scholar 

  • Fukazawa Y (1989) Antigenic structure of Candida albicans. Immunochemical basis of the serologic specificity of the mannans in yeasts. Immunol Ser 47:37–62

    PubMed  CAS  Google Scholar 

  • Fung JC, Donta ST, Tilton RC (1986) Candida detection system (CAND-TEC) to differentiate between Candida albicans colonization and disease. J Clin Microbiol 24:542–547

    PubMed  CAS  Google Scholar 

  • Girmenia C, Martino P, De Bernardis F, Cassone A (1997) Assessment of detection of Candida mannoproteinemia as a method to differentiate central venous catheter-related candidemia from invasive disease. J Clin Microbiol 35:903–906

    PubMed  CAS  Google Scholar 

  • Golbang N, Burnie JP, Matthews RC (1999) A polymerase chain reaction enzyme immunoassay for diagnosing infection caused by Aspergillus fumigatus. J Clin Pathol 52:419–423

    PubMed  CAS  Google Scholar 

  • Gold JW, Wong B, Bernard EM, Kiehn TE, Armstrong D (1983) Serum arabinitol concentrations and arabinitol/creatinine ratios in invasive candidiasis. J Infect Dis 147:504–513

    PubMed  CAS  Google Scholar 

  • Gutierrez J, Maroto C, Piedrola G, Martin E, Perez JA (1993) Circulating Candida antigens and antibodies: useful markers of candidemia. J Clin Mircobiol 31:2550–2552

    CAS  Google Scholar 

  • Harding SA, Brody JP, Normansell DE (1980) Antigenemia detected by enzyme-linked immunosorbent assay in rabbits with systemic candidiasis. J Lab Clin Med 95: 959–966

    PubMed  CAS  Google Scholar 

  • Hashiguchi K, Niki Y, Soejima R (1994) Cyclophosphamide induces false-positive results in detection of Aspergillus antigen in urine. Chest 105:975–976

    PubMed  CAS  Google Scholar 

  • Hashimoto A, Yamakami Y, Kamberi P, Yamagata E, Karashima R, Nagaoka H, Nasu M (1998) Comparison of PCR, (1,3)-beta-D-glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis. J Clin Lab Anal 12:257–262

    PubMed  CAS  Google Scholar 

  • Haynes K, Rogers TR (1994) Retrospective evaluation of a latex agglutination test for diagnosis of invasive aspergillosis in immunocompromised patients. Eur J Clin Microbiol Infect Dis 13:670–674

    PubMed  CAS  Google Scholar 

  • Haynes KA, Westerneng TJ, Fell JW, Moens W (1995) Rapid detection and identification of pathogenic fungi by polymerase chain reaction amplification of large subunit ribosomal DNA. J Med Vet Mycol 33:319–325

    PubMed  CAS  Google Scholar 

  • Herent P, Stynen D, Hernando F, Fruit J, Poulain D (1992) Retrospective evaluation of two latex agglutination tests for detection of circulating antigens during invasive candidosis. J Clin Microbiol 30:2158–2164

    PubMed  CAS  Google Scholar 

  • Hiyoshi M, Tagawa S, Hashimoto S, Sakamoto C, Tatsumi N (1999) Evaluation of a new laboratory test measuring plasma (1,3)-beta-D-glucan in the diagnosis of Candida deep mycosis: comparison with a serologic test. Kansenshogaku Zasshi 73:1–6

    PubMed  CAS  Google Scholar 

  • Holmes AR, Lee YC, Cannon RD, Jenkinson HF, Shepherd MG (1992) Yeast-specific DNA probes and their application for the detection of Candida albicans. J Med Microbiol 37:346–351

    PubMed  CAS  Google Scholar 

  • Hossain MA, Miyazaki T, Mitsutake K, Kakeya H, Yamamoto Y, Yanagihara K, Kawamura S, Otsubo T, Hirakata Y, Tashiro T, Kohno S (1997) Comparison between Wako-WB003 and Fungitec G tests for detection of (1,3)-beta-D-glucan in systemic mycoses. J Clin Lab Anal 11:73–77

    PubMed  CAS  Google Scholar 

  • Ikegami K, Ikemura K, Shimazu T, Shibuya M, Sugimoto H, Yoshioka T, Sugimoto T (1988) Early diagnosis of invasive candidiasis and rapid evaluation of antifungal therapy by combined use of conventional chromogenic limulus test and a newly developed endotoxin specific assay. J Trauma 28:1118–1126

    PubMed  CAS  Google Scholar 

  • Iwama A, Yoshida M, Miwa A, Obayshi T, Sakamoto S, Miura Y (1993) Improved survival from fungemia in patients with haematological malignancies: analysis of risk factors for death and usefulness of early antifungal therapy. Eur J Haematol 51:156–160

    PubMed  CAS  Google Scholar 

  • Jacquinot PM, Plancke Y, Sendid B, Strecker G, and Poulain D (1998) Nature of Candida albicans-derived carbohydrate antigen recognized by a monoclonal antibody in patients sera and distribution over Candida species. FEMS Microbiol Lett 166:131–138

    Google Scholar 

  • Jarvis WR (1995) Epidemiology of nosocomial fungal infections, with emphasis on Candida species. Clin Infect Dis 20:1526–1530

    PubMed  CAS  Google Scholar 

  • Jones ME, Fox AJ, Barnes AJ, Oppenheim BA, Balagopal P, Morgenstern GR, Scarffe JH (1998) PCR-ELISA for the early diagnosis of invasive pulmonary Aspergillus infection in neutropenic patients. J Clin Pathol 51:652–656

    PubMed  CAS  Google Scholar 

  • Jordan JA (1994) PCR identification of four medically important Candida species by using a single primer pair. J Clin Microbiol 32:2962–2967

    PubMed  CAS  Google Scholar 

  • Kahn FW, Jones JM (1986) Latex agglutination tests for detection of Candida antigens in sera of patients with invasive candidiasis. J Infect Dis 153:579–585

    PubMed  CAS  Google Scholar 

  • Kami M, Tanaka Y, Kanda Y, Ogawa S, Masumoto T, Ohtomo K, Matsumura T, Saito T, Machida U, Kashima T, Hirai H (2000) Computed tomographic scan of the chest, latex agglutination test and plasma 1,3-beta-D glucan assay in early diagnosis of invasive pulmonary aspergillosis: a prospective study of 215 patients. Haematologica 85:745–752

    PubMed  CAS  Google Scholar 

  • Kami M, Fukui T, Ogawa S, Kazuyama Y, Machida U, Tanaka Y, Kanda Y, Kashima T, Yamazaki Y, Hamaki T, Mori S, Akiyama H, Mutou Y, Sakamaki H, Osumi K, Kimura S, Hirai H (2001) Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis. Clin Infect Dis 33:1504–1512

    PubMed  CAS  Google Scholar 

  • Kan VL (1993) Polymerase chain reaction for the diagnosis of candidemia. J Infect Dis 168:779–783

    PubMed  CAS  Google Scholar 

  • Kappe R, Schulze-Berge A (1993) New cause for falsepositive results with the Pastorex Aspergillus antigen latex agglutination test. J Clin Microbiol 31:2489–2490

    PubMed  CAS  Google Scholar 

  • Kawamura S, Maesaki S, Noda T, Hirakata Y, Tomono K, Tashiro T, Kohno S (1999) Comparison between PCR and detection of antigen in sera for diagnosis of pulmonary aspergillosis. J Clin Microbiol 37:218–220

    PubMed  CAS  Google Scholar 

  • Kiehn TE, Bernard EM, Gold JW, Armstrong D (1979) Candidiasis: detection by gas-liquid chromatography of D-arabinitol, a fungal metabolite, in human serum. Science 206:577–580

    PubMed  CAS  Google Scholar 

  • Kuhlman JE, Fishman EK, Burch PA, Karp JE, Zerhouni EA, Siegelman SS (1987) Invasive pulmonary aspergillosis in acute leukemia — the contribution of CT to early diagnosis and aggressive management. Chest 92: 95–99

    PubMed  CAS  Google Scholar 

  • Larsson L, Pherson C, Wiebe T, Christensson B (1994) Gas chromatographic determination of D-arabinitol/Larabinitol ratios in urine: a potential method for diagnosis of disseminated candidiasis. J Clin Microbiol 32: 1855–1859

    PubMed  CAS  Google Scholar 

  • Lehmann PF, Reiss E (1980) Comparison by ELISA of serum anti-Candida albicans mannan IgG levels of a normal population and in diseased patients. Mycopathologia 70:89–93

    PubMed  CAS  Google Scholar 

  • Lehmann PF, Reiss E (1987) Invasive aspergillosis: antiserum for circulating antigen produced after immunization with serum from infected rabbits. Infect Immun 20:570–572

    Google Scholar 

  • Lehtonen L, Rantala A, Oksman P, Eerola E, Lehtonen OP (1993) Determination of serum arabinitol levels by mass spectrometry in patients with postoperative candidiasis. Eur J Clin Microbiol Infect Dis 12:330–335

    PubMed  CAS  Google Scholar 

  • Lehtonen L, Anttila VJ, Ruutu T, Salonen J, Nikoskelainen J, Eerola E, Ruutu P (1996) Diagnosis of disseminated candidiasis by measurement of urine D-arabinitol/Larabinitol ratio. J Clin Microbiol 34:2175–2179

    PubMed  CAS  Google Scholar 

  • Lemieux C, St-Germain G, Vincelette J, Kaufman L, and de Repentigny L (1990) Collaborative evaluation of antigen detection by a commercial latex agglutination test and enzyme immunoassay in the diagnosis of invasive candidiasis. J Clin Microbiol 28:249–253

    PubMed  CAS  Google Scholar 

  • Lescher-Bru V, Cavalier A, Pernot-Marino E, Koenig H, Eyer D, Waller J, Condolfi E (1998) Aspergillus galactomannan antigen detection with Platelia Aspergillus: multiple positive antigenemia without Aspergillus infection. J Mycol Med 8:112–113

    Google Scholar 

  • Lew MA, Siber GR, Donahue DM, Maiorca F (1982) Enhanced detection with an enzyme-linked immunosorbent assay of Candida mannan in antibodycontaining serum after heat extraction. J Infect Dis 145:45–56

    PubMed  CAS  Google Scholar 

  • Lischewski A, Amann RI, Harmsen D, Merkert DH, Hacker J, Morschhauser J (1996) Specific detection of Candida albicans and Candida tropicalis by fluorescent in situ hybridization with an 18S rRNA-targeted oligonucleotide probe. Microbiology 142:2731–2740

    PubMed  CAS  Google Scholar 

  • Loeffler J, Hebart H, Sepe S, Schumcher U, Klingebiel T, Einsele H (1998) Detection of PCR-amplified fungal DNA by using a PCR-ELISA system. Med Mycol 36: 275–279

    Google Scholar 

  • Loeffler J, Hebart H, Bialek R, Hagmeyer L, Schmidt D, Serey FP, Hartmann M, Eucker J, Einsele H (1999) Contaminations occurring in fungal PCR assays. J Clin Microbiol 37:1200–1202

    PubMed  CAS  Google Scholar 

  • Loeffler J, Henke N, Hebart H, Schmidt D, Hagmeyer L, Schumacher U, Einsele H (2000a) Quantification of fungal DNA by using fluorescence resonance energy transfer and the system. J Clin Microbiol 38:586–590

    PubMed  CAS  Google Scholar 

  • Loeffler J, Hebart H, Brauchle U, Schumacher U, Einsele HJ (2000b) Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR. Clin Microbiol 38:3830–3833

    CAS  Google Scholar 

  • Loeffler J, Hebart H, Cox P, Flues N, Schumacher U, Einsele H (2001) Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples. J Clin Microbiol 39:1626–1629

    PubMed  CAS  Google Scholar 

  • Machetti M, Feasi M, Mordini N, van Lint MT, Bacigalupo A, Latge JP, Sarfati J, Viscoli C (1998) Comparison of an enzyme immunoassay and a latex agglutination system for the diagnosis of invasive aspergillosis in bone marrow transplant recipients. Bone Marrow Transplant 21:917–921

    PubMed  CAS  Google Scholar 

  • Maertens J, Verhaegen J, Demuynck H, Brock P, Verhoef G, Vandenberghe P, van Eldere J, Verbist L, Boogaerts M (1999) Autopsy-controlled prospective evaluation of serial screening for circulating galactomannan by a sandwich enzyme-linked immunosorbent assay for hematological patients at risk for invasive aspergillosis. J Clin Microbiol 37:3223–3228

    PubMed  CAS  Google Scholar 

  • Manso E, Montillo M, de Sio G, D’Amico S, Discepoli G, Leoni P (1994) Value of antigen and antibody detection in the serological diagnosis of invasive aspergillosis in patients with hematological malignancies. Eur Clin Microbiol Infect Dis 13:756–760

    CAS  Google Scholar 

  • Marcilla A, Monteagudo C, Mormeneo S, Sentandreu R (1999) Monoclonal antibody 3H8: a useful tool in the diagnosis of candidiasis. Microbiology 145:695–701

    PubMed  CAS  Google Scholar 

  • Martin C, Roberts D, van der Weide M, Rossau R, Jannes G, Smith T, Maher M (2000) Development of a PCRbased line probe assay for identification of fungal pathogens. J Clin Microbiol 38:3735–3742

    PubMed  CAS  Google Scholar 

  • Mason AB, Brandt ME, Buckley HR (1989) Enolase activity associated with a C. albicans cytoplasmic antigen. Yeast 5:S231-S239

    PubMed  CAS  Google Scholar 

  • Matthews R, Burnie J (1988) Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen. J Clin Microbiol 26:259–263

    Google Scholar 

  • Matthews RC, Burnie JP, Tabaqchali S (1987) Isolation of immunodominant antigens from sera of patients with systemic candidiasis and characterization of serological response to Candida albicans. J Clin Microbiol 25:230–237

    PubMed  CAS  Google Scholar 

  • Meckstroth KL, Reiss E, Keller JW, Kaufman L (1981) Detection of antibodies and antigenemia in leukemic patients with candidiasis by enzyme-linked immunosorbent assay. J Infect Dis 144:24–32

    PubMed  CAS  Google Scholar 

  • Melchers WJ, Verweij PE, van den Hurk P, van Belkum A, De Pauw BE, Hoogkamp-Korstanje JA, Meis JF (1994) General primer-mediated PCR for detection of Aspergillus species. J Clin Microbiol 32:1710–1717

    PubMed  CAS  Google Scholar 

  • Miller WT (1996) Aspergillosis: a disease with many faces. Semin Roentgenol 31:52–66

    PubMed  CAS  Google Scholar 

  • Mitsutake K, Miyazaki T, Tashiro T, Yamamoto Y, Kakeya H, Otsubo T, Kawamura S, Hossain MA, Noda T, Hirakata Y, Kohno S (1996) Enolase antigen, mannan antigen, Cand-Tec antigen, and beta-glucan in patients with candidemia. J Clin Microbiol 34:1918–1921

    PubMed  CAS  Google Scholar 

  • Miyakawa Y, Mabuchi T, Kagaya K, Fukazawa Y (1992) Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction. J Clin Microbiol 30: 894–900

    PubMed  CAS  Google Scholar 

  • Miyakawa Y, Mabuchi T, and Fukazawa Y (1993) New method for detection of Candida albicans in human blood by polymerase chain reaction. J Clin Microbiol 31:3344–3347

    PubMed  CAS  Google Scholar 

  • Miyazaki T, Kohno S, Koga H, Kaku M, Mitsutake K, Maesaki S, Yasuoka A, Hara K, Tanaka S, Tamura H (1992) G test, a new direct method for diagnosis of Candida infection: comparison with assays for betaglucan and mannan antigen in a rabbit model of systemic candidiasis. J Clin Lab Anal 6:315–318

    PubMed  CAS  Google Scholar 

  • Miyazaki T, Kohno S, Mitsutake K, Maesaki S, Tanaka K, Ishikawa N, Hara K (1995) Plasma (1,3)-beta-Dglucan and fungal antigenemia in patients with candidemia, aspergillosis, and cryptococcosis. J Clin Microbiol 33:3115–3118

    PubMed  CAS  Google Scholar 

  • Montone KT, Litzky LA (1995) Rapid method for detection of Aspergillus 5S ribosomal RNA using a genusspecific oligonucleotide probe. J Clin Pathol 103:48–51

    CAS  Google Scholar 

  • Morace G, Pagano L, Sanguinetti M, Posteraro B, Mele L, Equitani F, D’Amore G, Leone G, Fadda G (1999) PCRrestriction enzyme analysis for detection of Candida DNA in blood from febrile patients with hematological malignancies. J Clin Microbiol 37:1871–1875

    PubMed  CAS  Google Scholar 

  • Mori T, Matsumura M (1999) Clinical evaluation of diagnostic methods using plasma and/or serum for three mycoses: aspergillosis, candidosis, and pneumocystosis. Nippon Ishinkin Gakkai Zasshi 40:223–230

    CAS  Google Scholar 

  • Mori T, Ikemoto H, Matsumura M, Yoshida M, Inada K, Endo S, Ito A, Watanabe S, Yamaguchi H, Mitsuya M, Kodama M, Tani T, Yokota T, Kobayashi T, Kambayashi J, Nakamura T, Masaoka T, Teshima H, Yoshinaga T, Kohno S, Hara K, Miyazaki S (1997) Evaluation of plasma (1,3)-beta-D-glucan measurement by the kinetic turbidimetric Limulus test, for the clinical diagnosis of mycotic infections. Eur J Clin Chem Clin Biochem 35:553–560

    PubMed  CAS  Google Scholar 

  • Nakamura A, Ishikawa N, Suzuki H (1991) Diagnosis of invasive candidiasis by detection of mannan antigen by using the avidin-biotin enzyme immunoassay. J Clin Microbiol 29:2363–2367

    PubMed  CAS  Google Scholar 

  • Nakamura H, Y Shibata Y, Kudo Y, Saito S, Kimura H, and Tomoike H (1994) Detection of Aspergillus fumigatus DNA by polymerase chain reaction in the clinical samples from individuals with pulmonary aspergillosis. Rhinsho-Byori 42:676–681

    CAS  Google Scholar 

  • Nelson GE, Johnson DE, Ciegler A (1971) Production of D-mannitol by conidia of Aspergillus candidus. Appl Microbiol 22:484–485

    PubMed  CAS  Google Scholar 

  • Obayshi T, Yoshida M, Tamura H, Aketagawa J, Tanaka S, Kawai T (1992) Determination of plasma (1,3)-betaD-glucan: a new diagnostic aid to deep mycosis. J Med Vet Mycol 30:275–280

    Google Scholar 

  • Obayshi T, Yoshida M, Mori T, Goto H, Yasuoka A, Iwasaki H, Teshima H, Kohno S, Horiuchi A, Ito A (1995) Plasma (1,3)-beta-D-glucan determination in the diagnosis of invasive deep mycosis and fungal febrile episodes. Lancet 345:17–20

    Google Scholar 

  • Onishi H, Suzuki T (1968) Production of D-mannitol and glycerol by yeasts. Appl Microbiol 16:1847–1852

    PubMed  CAS  Google Scholar 

  • Park CS, Kim J, Montone KT (1997) Detection of Aspergillus ribosomal RNA using biotinylated oligonucleotide probes. Diagn Mol Pathol 6:255–260

    PubMed  CAS  Google Scholar 

  • Pfaller MA, Cabezudo I, Bushelman B, Bale M, Howe T, Vitug M, Linton HJ, Densel M (1993) Value of the Hybritech ICON Candida Assay in the diagnosis of invasive candidiasis in high-risk patients. Diagn Microbiol Infect Dis 16:53–60

    PubMed  CAS  Google Scholar 

  • Phillips P, Dowd A, Jewesson P, Radigan G, Tweeddale MG, Clarke A, Greere I, Kelly M (1990) Nonvalue of antigen detection immunoassays for diagnosis of candidemia. J Clin Microbiol 28:2320–2326

    PubMed  CAS  Google Scholar 

  • Rand KH, Houck H, Wolff M (1994) Detection of candidemia by polymerase chain reaction. Mol Cell Probes 8:215–221

    PubMed  CAS  Google Scholar 

  • Reiss E, Lehmann PF (1979) Galactomannan antigenemia in invasive aspergillosis. Infect Immun 25:357–365

    PubMed  CAS  Google Scholar 

  • Reiss E, Stockman L, Kuykendall RJ, Smith SJ (1982) Dissociation of mannan — serum complexes and detection of Candida albicans mannan by enzyme immunoassay variations. Clin Chem 28:306–310

    PubMed  CAS  Google Scholar 

  • Reiss E, Tanaka K, Bruker G, Chazalet V, Coleman D, Debeaupuis JP, Hanazawa R, Latge JP, Lortholary J, Makimura K, Morrison CJ, Murayama SY, Naoe S, Paris S, Sarfati J, Shibuya K, Sullivan D, Uchida K, Yamaguchi H (1998) Molecular diagnosis and epidemiology of fungal infections. Med Mycol 36:249–257

    PubMed  CAS  Google Scholar 

  • Roboz J, Katz RN (1992) Diagnosis of disseminated candidiasis based on serum D/L-arabinitol ratios using negative chemical ionization mass spectrometry. J Chromatogr 575:281–286

    PubMed  CAS  Google Scholar 

  • Roboz J, Suzuki R, Holland JF (1980) Quantification of arabinitol in serum by selected ion monitoring as a diagnostic technique in invasive candidiasis. J Clin Microbiol 12:594–602

    PubMed  CAS  Google Scholar 

  • Roboz J, Kappatos DC, Greaves J, Holland JF (1984) Determination of polyols in serum by selected ion monitoring. Clin Chem 30:1611–1615

    PubMed  CAS  Google Scholar 

  • Roboz J, Kappatos DC, Holland JF (1987) Role of individual serum pentitol concentration in the diagnosis of disseminated visceral candidiasis. Eur J Clin Microbiol 6:708–714

    PubMed  CAS  Google Scholar 

  • Roboz J, Nieves E, Holland JF (1990) Separation and quantification by gas chromatography-mass spectrometry of arabinitol enantiomers to aid the differential diagnosis of disseminated candidiasis. J Chromatogr 500: 413–426

    PubMed  CAS  Google Scholar 

  • Rogers TR, Haynes KA, Barnes RA (1990) Value of antigen detection in predicting invasive pulmonary aspergillosis. Lancet 336:1210–1213

    PubMed  CAS  Google Scholar 

  • Rohrlich P, Sarfati J, Mariani P, Duval M, Carol A, SaintMartin C, Bingen E, Latge JP, Vilmer E (1996) Prospective sandwich enzyme-linked immunosorbent assay for serum galactomannan: early predictive value and clinical use in invasive aspergillosis. Pediatr Infect Dis J 15:232–237

    PubMed  CAS  Google Scholar 

  • Sabetta JR, Miniter P, Andriole VT (1985) The diagnosis of invasive aspergillosis by an enzyme-linked immunosorbent assay for circulating antigen. J Infect Dis 152:946–953

    PubMed  CAS  Google Scholar 

  • Sakai T, Ikegami K, Yoshinaga E, Uesugi-Hayakawa R, Wakizaka A (2000) Rapid, sensitive and simple detection of Candida deep mycosis by amplification of 18S ribosomal RNA gene; comparison with assay of serum beta-D-glucan level in clinical samples. Tohoku J Exp Med 190:119–128

    PubMed  CAS  Google Scholar 

  • Salonen JH, Rimpilainen M, Lehtonen L, Lehtonen OP, Nikoskelainen J (2001) Measurement of the Darabinitol/L-arabinitol ratio in urine of neutropenic patients treated empirically with amphotericin B. Eur J Clin Microbiol Infect Dis 20:179–184

    PubMed  CAS  Google Scholar 

  • Sanchez ML, Pfaller MA, Cabezudo I, Bale M, Buschelman B (1992) Diagnosis of disseminated candidiasis in hospitalized patients using the Cand-Tec latex agglutination assay. Mycopathologia 118:153–162

    PubMed  CAS  Google Scholar 

  • Sandhu GS, Kline BC, Stockman L, Roberts GD (1995) Molecular probes for diagnosis of fungal infections. J Clin Microbiol 33:2913–2919

    PubMed  CAS  Google Scholar 

  • Schonheyder H (1987) Pathogenetic and serological aspects of pulmonary aspergillosis. Scand J Infect Dis 51(Suppl):1–62

    CAS  Google Scholar 

  • Sendid B, Tabouret M, Poirot JL, Mathieu D, Fruit J, Poulain D (1999) New enzyme immunoassays for sensitive detection of circulating Candida albicans mannan and antimannan antibodies: useful combined test for diagnosis of systemic candidiasis. J Clin Microbiol 37:1510–1517

    PubMed  CAS  Google Scholar 

  • Siemann M, Koch-Dorfler M, Gaude M (1998) False-positive results in premature infants with the Platelia Aspergillus sandwich enzyme-linked immunosorbent assay. Mycoses 41:373–377

    PubMed  CAS  Google Scholar 

  • Sigmundsdottir G, Christensson B, Bjorklund LJ, Hakansson K, Peherson C, Larsson L (2000) Urine Darabinitol/L-arabinitol ratio in diagnosis of invasive candidiasis in newborn infants. J Clin Mircrobiol 38:3039–3042

    CAS  Google Scholar 

  • Skladny H, Buchheidt D, Baust C, Krieg-Schneider F, Seifarth W, Leib-Mosch C, Hehlmann R (1999) Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR. J Clin Microbiol 37: 3865–3871

    PubMed  CAS  Google Scholar 

  • Soyama K, Ono E (1985) Enzymatic fluorimetric method for the determination of D-arabinitol in serum by initial rate analysis. Clin Chim Acta 149:149–154

    PubMed  CAS  Google Scholar 

  • Soyama K, Ono E (1987) Improved procedure for determining D-arabinitol by resazurin-coupled method. Clin Chim Acta 168:259–260

    PubMed  CAS  Google Scholar 

  • Spreadbury C, Holden D, Aufauvre-Brown A, Bainbridge B, Cohen J (1993) Detection of Aspergillus fumigatus by polymerase chain reaction. J Clin Microbiol 31:615–621

    PubMed  CAS  Google Scholar 

  • Stynen D, Goris A, Sarfati J, Latge JP (1995) A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis. J Clin Microbiol 33:497–500

    PubMed  CAS  Google Scholar 

  • Sulahian A, Tabouret M, Ribaud P, Sarfati J, Gluckman E, Latge JP, Derouin F (1996) Comparison of an enzyme immunoassay and latex agglutination test for detection of galactomannan in the diagnosis of invasive aspergillosis. Eur J Clin Microbiol Infect Dis 15: 139–145

    PubMed  CAS  Google Scholar 

  • Sundstrom P, Aliaga GR (1994) A subset of proteins found in culture supernatants of Candida albicans includes the abundant, immunodominant, glycolytic enzyme enolase. J Infect Dis 169:452–456

    PubMed  CAS  Google Scholar 

  • Swanink CN, Meis JF, Rijs AJ, Donnelly JP, Verweij PE (1997) Specificity of a sandwich enzyme-linked immunosorbent assay for detection of Aspergillus galactomannan. J Clin Microbiol 35:257–260

    PubMed  CAS  Google Scholar 

  • Switchenko AC, Miyada CG, Goodman TC, Walsh TJ, Wong B, Becker MJ, Ullman EF (1994) An automated enzymatic method for the measurement of D-arabinitol, a metabolite of pathogenic Candida species. J Clin Microbiol 32:92–97

    PubMed  CAS  Google Scholar 

  • Talbot GH, Weiner MH, Gerson SL, Provencher M, Hurwitz S (1987) Serodiagnosis of invasive aspergillosis in patients with hematologic malignancy: validation of the Aspergillus fumigatus antigen radioimmunoassay. J Infect Dis 155:12–27

    PubMed  CAS  Google Scholar 

  • Tang CM, Holden DW, Aufauvre-Brown A, Cohen J (1993) The detection of Aspergillus spp. by the polymerase chain reaction and its evaluation in bronchoalveolar lavage fluid. Am Rev Respir Dis 148:1313–1317

    PubMed  CAS  Google Scholar 

  • Taschdjian CL, Kozinn PJ, Toni EF (1970) Opportunistic yeast infections, with special reference to candidiasis. Ann NY Acad Sci 174:606–622

    PubMed  CAS  Google Scholar 

  • Tokunaga S, Ohkawa M, Takashima M, Hisazumi H (1992) Clinical significance of measurement of serum Darabinitol levels in candiduria patients. Urol Int 48:195–199

    PubMed  CAS  Google Scholar 

  • Tokunaga S, Ohkawa M, Takashima M, Seto C, Nakamura S (1995) D-arabinitol versus mannan antigen and Candidal protein antigen as a serum marker for Candida pyelonephritis. Eur J Clin Microbiol Infect Dis 14: 118–121

    PubMed  CAS  Google Scholar 

  • Touster O, Shaw DRD (1962) Biochemistry of the acyclic polyols. Physiol Rev 42:181–225

    PubMed  CAS  Google Scholar 

  • Tregar TR, Visscher DW, Bartlett MS, Smith JW (1985) Diagnosis of pulmonary infection caused by Aspergillus: usefulness of respiratory cultures. J Infect Dis 152:572–576

    Google Scholar 

  • Ulusakarya A, Chachaty E, Vantelon JM, Youssef A, Tancrede C, Pico JL, Bourhis JH, Fenaux P, Munck JN (2000) Surveillance of Aspergillus galactomannan antigenemia for invasive aspergillosis by enzymelinked immunosorbent assay in neutropenic patients treated for hematological malignancies. Hematol J 1:111–116

    PubMed  CAS  Google Scholar 

  • Urata T, Kobayashi M, Imamura J, Tanaka Y, Muneishi H, Iwahara Y, Uemura Y, Taguchi H, Miyoshi I (1997) Polymerase chain reaction amplification of Asp f I and alkaline protease genes from fungus balls: clinical application in pulmonary aspergillosis. Intern Med 36:19–27

    PubMed  CAS  Google Scholar 

  • Van Deventer AJ, Goessens WHF, van Belkum A, van Vliet HJA, van Etten EWM, Verbrugh HA (1995) Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis. J Clin Microbiol 33:625–628

    PubMed  CAS  Google Scholar 

  • Vazquez JS (1994) Use of the polymerase chain reaction for the diagnosis of invasive Candida infection. Serodiagn Immunother Infect Dis 6:173–178

    Google Scholar 

  • Verweij PE, Rijs AJ, de Pauw BE, Horrevorts AM, Hoogkamp-Korstanje JA, Meis JF (1995a) Clinical evaluation and reproducibility of the Pastorex Aspergillus antigen latex agglutination test for diagnosing invasive aspergillosis. J Clin Pathol 48:474–476

    PubMed  CAS  Google Scholar 

  • Verweij PE, Stynen D, Rijs AJ, de Pauw BE, HoogkampKorstanje JA, Meis JF (1995b) Sandwich enzymelinked immunosorbent assay compared with Pastorex latex agglutination test for diagnosing invasive aspergillosis in immunocompromised patients. J Clin Microbiol 33:1912–1914

    PubMed  CAS  Google Scholar 

  • Verweij PE, Donnelly JP, De Pauw BE, Meis JF (1996) Prospects for the early diagnosis of invasive aspergillosis in the immunocompromised patients. Rev Med Microbiol 7:105–113

    Google Scholar 

  • Verweij PE, Brinkman K, Kremer HP, Kullberg BJ, Meis JF (1999) Aspergillus meningitis: diagnosis by nonculture-based microbiological methods and management. J Clin. Microbiol 37:1186–1189

    PubMed  CAS  Google Scholar 

  • Wahyuningsih R, Freisleben HJ, Sonntag HG, Schnitzler P (2000) Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis. J Clin Microbiol 38:3016–3021

    PubMed  CAS  Google Scholar 

  • Walsh TJ, Chanock SJ (1998) Diagnosis of invasive fungal infections: advances in nonculture systems. Curr Clin Top Infect Dis 18:101–153

    PubMed  CAS  Google Scholar 

  • Walsh TJ, Hathorn JW, Sobel JD, Merz WG, Sanchez V, Maret SM, Buckley HR, Pfaller MA, Schaufele R, Sliva C (1991) Detection of circulating Candida enolase by immunoassay in patients with cancer and invasive candidiasis. N Engl J Med 324:1026–103

    PubMed  CAS  Google Scholar 

  • Walsh TJ, Merz WG, Lee JW, Schaufele R, Sein T, Whitcomb P, Ruddel M, Burns W, Wingard JR, Switchenko AC (1995) Diagnosis and therapeutic monitoring of invasive candidiasis by rapid enzymatic detection of serum D-arabinitol. Am J Med 99:164–172

    PubMed  CAS  Google Scholar 

  • Weiner MH, Coats-Stephen M (1979) Immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections. J Infect Dis 140:989–993

    PubMed  CAS  Google Scholar 

  • Williamson EC, Leeming JP, Palmer HM, Steward CG, Warnock D, Marks DI, Millar MR (2000) Diagnosis of invasive aspergillosis in bone marrow transplant recipients by polymerase chain reaction. Br J Haematol 108:132–139

    PubMed  CAS  Google Scholar 

  • Wilson EV, Hearn VM, MacKenzie DR (1987) Evaluation of a test to detect circulating Aspergillus fumigatus antigen in a survey of immunocompromised patients with proven or suspected invasive disease. J Med Vet Mycol 25:365–374

    PubMed  CAS  Google Scholar 

  • Wojnarowski W, Jaquet H, Glauser MP (1979) Arabinitol, a metabolite of Candida albicans (abstract). Experientia 35:945

    Google Scholar 

  • Wong B, Castellanos M (1989) Enantioselective measurement of the Candida metabolite D-arabinitol in human serum using multidimensional gas chromatography and a new chiral phase. J Chromatogr 495:21–30

    PubMed  CAS  Google Scholar 

  • Wong B, Bernard EM, Gold JW, Fong D, Armstrong D (1982a) The arabinitol appearance rate in laboratory animals and humans: estimation from the arabinitol/creatinine ratio and relevance to the diagnosis of candidiasis. J Infect Dis 146:353–359

    PubMed  CAS  Google Scholar 

  • Wong B, Bernard EM, Gold JWM, Fong D, Silber A, Armstrong D (1982b) Increased arabinitol levels in experimental candidiasis in rats: arabinitol appearance rates, arabinitol/creatinine ratios, and severity of infection. J Infect Dis 146: 346–352

    PubMed  CAS  Google Scholar 

  • Wong B, Brauer KL, Tsai RR, Jayasimhulu K (1989) Increased amounts of the Aspergillus metabolite Dmannitol in tissue and serum of rats with experimental aspergillosis. J Infect Dis 160:95–103

    PubMed  CAS  Google Scholar 

  • Wong B, Brauer KL, Clemens JR, Beggs S (1990) Effects of gastrointestinal candidiasis, antibiotics, dietary arabinitol, and cortisone acetate on levels of the Candida metabolite D-arabinitol in rat serum and urine. Infect Immun 58:283–288

    PubMed  CAS  Google Scholar 

  • Yamakami Y, Hashimoto A, Tokimatsu I, Nasu M (1996) PCR detection of DNA specific for Aspergillus species in serum of patients with invasive aspergillosis. J Clin Microbiol 34:2464–2468

    PubMed  CAS  Google Scholar 

  • Yamakami Y, Hashimoto A, Yamagata E, Kamberi P, Karashima R, Nagai H, Nasu M. (1998) Evaluation of PCR for detection of DNA specific for Aspergillus species in sera of patients with various forms of pulmonary aspergillosis. J Clin Microbiol 36:3619–3623

    PubMed  CAS  Google Scholar 

  • Yeo SF, Zhang Y, Shafer D, Campbell S, Wong B (2000) A rapid, automated enzymatic fluorometric assay for determination of D-arabinitol in serum. J Clin Microbiol 38:1439–1443

    PubMed  CAS  Google Scholar 

  • Yera H, Sendid B, Francois N, Camus D, Poulain D (2001) Contribution of serological tests and blood culture to the early diagnosis of systemic candidiasis. Eur J Clin Microbiol Infect Dis 20:864–870

    PubMed  CAS  Google Scholar 

  • Yoshida M, Obayashi T, Iwama A, Ito M, Tsunoda S, Suzuki T, Muroi K, Ohta M, Sakamoto S, Miura Y (1997) Detection of plasma (1,3)-beta-D-glucan in pateints with Fusarium, Trichosporon, Saccharomyces and Acremonium fungaemias. J Med Vet Mycol 35:371–374

    PubMed  CAS  Google Scholar 

  • Young RC, Bennett JE (1971) Invasive aspergillosis: absence of detectable antibody response. Am Rev Respir Dis 104:710–716

    PubMed  CAS  Google Scholar 

  • Yu VL, Muder RR, Poorsattar A (1986) Significance of isolation of Aspergillus from the respiratory tract in diagnosis of invasive pulmonary aspergillosis. Am J Med 81:249–254

    PubMed  CAS  Google Scholar 

  • Yuasa K, Goto H, Iguchi M, Okamura T, Ieki R (1996) Evaluation of the diagnostic value of the measurement of 1,3-beta-D-glucan in patients with pulmonary aspergillosis. Respiration 63:78–83

    PubMed  CAS  Google Scholar 

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  1. Infectious Diseases Section, Department of Medicine, Yale University School of Medicine, New Haven, Connecticut, 06510, USA

    Samuel A. Lee & Brian Wong

  2. Infectious Diseases Section, VA Connecticut Healthcare System, 950 Campbell Ave., (111-I), West Haven, Connecticut, 06516, USA

    Samuel A. Lee & Brian Wong

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  1. Samuel A. Lee
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  1. Cratis D. Williams Graduate School, Appalachian State University, Boone, North Carolina, 28607, USA

    Judith E. Domer (Dean of Graduate Studies and Research) (Dean of Graduate Studies and Research)

  2. Department of Medicine, Washington University School of Medicine, Box 8051, 660 South Euclid Avenue, St. Louis, Missouri, 63110, USA

    George S. Kobayashi

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Lee, S.A., Wong, B. (2004). Advances in Diagnostic Methods for Invasive Candida and Aspergillus Infections. In: Domer, J.E., Kobayashi, G.S. (eds) Human Fungal Pathogens. The Mycota, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10380-7_3

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