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The gp43 from Paracoccidioides brasiliensis: A Major Diagnostic Antigen and Vaccine Candidate

  • Luiz R. Travassos
  • Carlos P. Taborda
  • Leo K. Iwai
  • Ed Cio Cunha-Neto
  • Rosana Puccia
Part of the The Mycota book series (MYCOTA, volume 12)

Abstract

The gp43, a 43,000 dalton glycoprotein from Paracoccidioides brasiliensis, is one of those rare examples of a major diagnostic antigen in serological assays (e.g., Mendes-Giannini et al. 1990) that also contains epitopes eliciting an immunoprotective cellular immune response. Its role in fungal virulence is not clearly defined which raises the ques-tion as to the selective pressure that keeps its expression constant in both yeast and mycelium phase of this dimorphic pathogenic species.

Keywords

Gp43 Gene Capture Enzyme Immunoassay Gp43 Isoforms Peripheral Blood Mononudear Cell Major Diagnostic Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Almeida IC, Neville DC, Mehlert A, Treumann A, Ferguson MA, Previato JO, Travassos LR (1996) Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis. Glycobiology 6:507–515PubMedCrossRefGoogle Scholar
  2. Almeida SR, Moraes JZ, Camargo ZP, Gesztesi JL, Mariano M, Lopes JD (1998a) Pattern of immune response to gp43 from Paracoccidioides brasiliensis in susceptible and resistant mice is influenced by antigen-presenting cells. Cell Immunol 190:68–76PubMedCrossRefGoogle Scholar
  3. Almeida SR, Unterkircher CS, Camargo ZP (1998b) Involvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophages. Med Mycol 36:405–411PubMedCrossRefGoogle Scholar
  4. Bava AJ, Mistchenko AS, Palacios MF, Estevez ME, Tiraboschi NI, Sen L, Negroni R, Diez RA (1991) Lymphocyte subpopulations and cytokine production in paracoccidioidomycosis patients. Microbiol Immunol 35:167–174PubMedGoogle Scholar
  5. Benard G, Romano CC, Cacere CR, Juvenale M, Mendes-Giannini MJ, Duarte AJ (2001) Imbalance of IL-2, IFN-gamma and IL-10 secretion in the immunosuppression associated with human paracoccidioidomycosis. Cytokine 13:248–252PubMedCrossRefGoogle Scholar
  6. Benkirane N, Friede M, Guichard G, Briand JP, Van Regenmortel MH, Muller S (1993) Antigenicity and immunogenicity of modified synthetic peptides containing D-amino acid residues. Antibodies to a D-enantiomer do recognize the parent L-hexapeptide and reciprocally. J Biol Chem 268:26279–26285PubMedGoogle Scholar
  7. Bercovici N, Heurtier A, Vizler C, Pardigon N, Cambouris C, Desreumaux P, Liblau R (2000) Systemic administration of agonist peptide blocks the progression of spontaneous CD8-mediated autoimmune diabetes in transgenic mice without bystander damage. J Immunol 165:202–210PubMedGoogle Scholar
  8. Biselli PJ, Juvenale M, Mendes-Giannini MJ, Duarte AJ, Benard G (2001) IgE antibody response to the main antigenic component of Paracoccidioides brasiliensis in patients with paracoccidioidomycosis. Med Mycol 39:475–478PubMedGoogle Scholar
  9. Blotta MH, Camargo ZP (1993) Immunological response to cell-free antigens of Paracoccidioides brasiliensis: relationship with clinical forms of paracoccidioidomycosis. J Clin Microbiol 31:671–676PubMedGoogle Scholar
  10. Brummer E, Hanson LH, Restrepo A, Stevens DA (1988) In vivo and in vitro activation of pulmonary macrophages by IFN-gamma for enhanced killing of Paracoccidioides brasiliensis or Blastomyces dermatitidis. J Immunol 140:2786–2789PubMedGoogle Scholar
  11. Calich VL, Singer-Vermes LM, Russo M, Vaz CAC, Burger E (1994) Immunogenetics in paracoccidioidomycosis. In: Franco M, Lacaz CS, Restrepo-Moreno A, Del Negro G (eds) Paracoccidioidomycosis. CRC Press, Boca Raton, pp 151–173Google Scholar
  12. Camargo ZP, Franco MF (2000) Current knowledge on pathogenesis and immunodiagnosis of paracoccidioidomycosis. Rev Iberoam Micol 17:41–48.PubMedGoogle Scholar
  13. Camargo Z, Unterkircher C, Campoy SP, Travassos LR (1988) Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol 26:2147–2151PubMedGoogle Scholar
  14. Camargo ZP, Unterkircher C, Travassos LR (1989) Identification of antigenic polypeptides of Paracoccidioides brasiliensis by immunoblotting. J Med Vet Mycol 27: 407–412PubMedCrossRefGoogle Scholar
  15. Camargo ZP, Gesztesi JL, Saraiva EC, Taborda CP, Vicentini AP, Lopes JD (1994) Monoclonal antibody capture enzyme immunoassay for detection of Paracoccidioides brasiliensis antibodies in paracoccidioidomycosis. J Clin Microbiol 32:2377–2381PubMedGoogle Scholar
  16. Cano LE, Kashino SS, Arruda C, Andre D, Xidieh CF, Singer-Vermes LM, Vaz CA, Burger E, Calich VL (1998) Protective role of gamma interferon in experimental pulmonary paracoccidioidomycosis. Infect Immun 66:800–806PubMedGoogle Scholar
  17. Cano MI, Cisalpino PS, Galindo I, Ramirez JL, Mortara RA, da Silveira JF (1998) Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis. J Clin Microbiol 36:742–747PubMedGoogle Scholar
  18. Carmona AK, Puccia R, Oliveira MC, Rodrigues EG, Juliano L, Travassos LR (1995) Characterization of an exocellular serine-thiol proteinase activity in Paracoccidioides brasiliensis. Biochem J 309:209–214PubMedGoogle Scholar
  19. Cisalpino PS, Puccia R, Yamauchi LM, Cano MI, da Silveira JF, Travassos LR (1996) Cloning, characterization, and epitope expression of the major diagnostic antigen of Paracoccidioides brasiliensis. J Biol Chem 271: 4553–4560PubMedCrossRefGoogle Scholar
  20. Cunha-Neto E (1999) MHC-restricted antigen presentation and recognition: constraints on gene, recombinant and peptide vaccines in humans. Braz J Med Biol Res 32:199–205PubMedCrossRefGoogle Scholar
  21. Deepe GS, Romani L, Calich VL, Huffnagle G, Arruda C, Molinari-Madlum EE, Perfect JR (2000) Knockout mice as experimental models of virulence. Med Mycol 38:87–98PubMedGoogle Scholar
  22. Diniz SN, Carvalho KC, Cisalpino PS, Silveira JF, Travassos LR, Puccia R (2002) Expression in bacteria of the gene encoding the gp43 antigen of Paracoccidioides brasiliensis: Immunological reactivity of the recombinant fusion proteins. Clin Diag Lab Immunol 9:1200–1204Google Scholar
  23. Engler OB, Dai WJ, Sette A, Hunziker IP, Reichen J, Pichler WJ, Cerny A (2001) Peptide vaccines against hepatitis B virus: from animal model to human studies. Mol Immunol 38:457–465PubMedCrossRefGoogle Scholar
  24. Feitosa LS (2000) Polimorfismos cromossômicos do Paracoccidioides brasiliensis e mapeamento dos genes com sondas homólogas. MS Thesis, Universidade Federal de São Paulo, SP, BrazilGoogle Scholar
  25. Gesztesi JL, Puccia R, Travassos LR, Vicentini AP, de Moraes JZ, Franco MF, Lopes JD (1996) Monoclonal antibodies against the 43,000 Da glycoprotein from Paracoccidioides brasiliensis modulate laminin-mediated fungal adhesion to epithelial cells and pathogenesis. Hybridoma 15:415–422PubMedCrossRefGoogle Scholar
  26. Gonzalez A, de Gregori W, Velez D, Restrepo A, Cano LE (2000) Nitric oxide participation in the fungicidal mechanism of gamma interferon-activated murine macrophages against Paracoccidioides brasiliensis conidia. Infect Immun 68:2546–2552PubMedCrossRefGoogle Scholar
  27. Gurunathan S, Klinman DM, Seder RA (2000) DNA vaccines: immunology, application, and optimization. Annu Rev Immunol 18:927–74PubMedCrossRefGoogle Scholar
  28. Hammer J, Sturniolo T, Sinigaglia F (1997) HLA class II peptide binding specificity and autoimmunity. Adv Immunol 66:67–100PubMedCrossRefGoogle Scholar
  29. Hanna SA, Monteiro da Silva JL, Giannini MJ (2000) Adherence and intracellular parasitism of Paracoccidioides brasiliensis in Vero cells. Microbes Infect 2:877–884PubMedCrossRefGoogle Scholar
  30. Iwai LK, Yoshida M, Marin ML, Juliano MA, Hammer J, Shikanai-Yasuda MA, Juliano L, Goldberg AC, Kalil J, Travassos LR, Cunha Neto E (2001) Selection of potential vaccine T cell epitopes from gp43 of Paracoccidioides brasiliensis based on prediction of peptide binding to multiple HLA molecules. 11th International Congress of Immunology, Scand J Immunol 54 (Suppl 1), Abstract A5.Mon.5.1/1270Google Scholar
  31. Jiang C, Magee DM, Quitugua TN, Cox RA (1999a) Genetic vaccination against Coccidioides immitis: comparison of vaccine efficacy of recombinant antigen 2 and antigen 2 cDNA. Infect Immun 67:630–635PubMedGoogle Scholar
  32. Jiang C, Magee DM, Cox RA (1999b) Coadministration of interleukin 12 expression vector with Antigen 2 cDNA enhances induction of protective immunity against Coccidioides immitis. Infect Immun 67:5848–5853PubMedGoogle Scholar
  33. Karhawi AS, Colombo AL, Salomao R (2000) Production of IFN-gamma is impaired in patients with paracoccidioidomycosis during active disease and is restored after clinical remission. Med Mycol 38:225–229PubMedGoogle Scholar
  34. Kurita N, Oarada M, Miyaji M, Ito E (2000) Effect of cytokines on antifungal activity of human polymorphonuclear leucocytes against yeast cells of Paracoccidioides brasiliensis. Med Mycol 38:177–182PubMedGoogle Scholar
  35. McEwen JG, Restrepo BI, Salazar ME, Restrepo A (1987) Nuclear staining of Paracoccidioides brasiliensis conidia. J Med Vet Mycol 25:343–345PubMedCrossRefGoogle Scholar
  36. McGowan KL, Buckley HR (1985) Preparation and use of cytoplasmic antigens for the serodiagnosis of paracoccidioidomycosis. J Clin Microbiol 22:39–43PubMedGoogle Scholar
  37. Mendes-Giannini MJ, Bueno JP, Shikanai-Yasuda MA, Ferreira AW, Masuda A (1989) Detection of the 43,000molecular-weight glycoprotein in sera of patients with paracoccidioidomycosis. J Clin Microbiol 27:2842–2845PubMedGoogle Scholar
  38. Mendes-Giannini MJS, Bueno JP, Shikanai-Yasuda MA, Stolf AMS, Masuda A, Amato-Neto V, Ferreira AW (1990) Antibody response to the 43 kDa glycoprotein of Paracoccidioides brasiliensis as a marker for the evaluation of patients under treatment. Am J Trop Med Hyg 43:200–206Google Scholar
  39. Molinari-Madlum EE, Felipe MS, Soares CM (1999) Virulence of Paracoccidioides brasiliensis isolates can be correlated to groups defined by random amplified polymorphic DNA analysis. Med Mycol 37:269–276PubMedCrossRefGoogle Scholar
  40. Morais FV, Barros TF, Fukada MK, Cisalpino PS, Puccia R (2000) Polymorphism in the gene coding for the immunodominant antigen gp43 from the pathogenic fungus Paracoccidioides brasiliensis. J Clin Microbiol 38:3960–3966PubMedGoogle Scholar
  41. Moscardi-Bacchi M, Brummer E, Stevens DA (1994) Support of Paracoccidioides brasiliensis multiplication by human monocytes or macrophages: inhibition by activated phagocytes. J Med Microbiol 40:159–164PubMedCrossRefGoogle Scholar
  42. Moura-Campos C, Gesztesi JL, Vincentini AP, Lopes JD, Camargo ZP (1995) Expression and isoforms of gp43 in different strains of Paracoccidioides brasiliensis. J Med Vet Mycol 33:223–227CrossRefGoogle Scholar
  43. Muller GM, Shapira M, Arnon R (1982) Anti-influenza response achieved by immunization with a synthetic conjugate. Proc Natl Acad Sci USA 79:569–573PubMedCrossRefGoogle Scholar
  44. Nascimento FR, Calich VL, Rodriguez D, Russo M (2002) Dual role for nitric oxide in paracoccidioidomycosis: essential for resistance, but overproduction associated with susceptibility. J Immunol 168:4593–4600PubMedGoogle Scholar
  45. Patarroyo ME, Amador R, Clavijo P, Moreno A, Guzman F, Romero P, Tascon R, Franco A, Murillo LA, Ponton G (1988) A synthetic vaccine protects humans against challenge with asexual blood stages of Plasmodium falciparum malaria. Nature 332:158–161PubMedCrossRefGoogle Scholar
  46. Pinto AR, Puccia R, Diniz SN, Franco MF, Travassos LR (2000) DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from Paracoccidioides brasiliensis. Vaccine 18:3050–3058PubMedCrossRefGoogle Scholar
  47. Posnett DN, McGrath H, Tam JP (1988) A novel method for producing anti-peptide antibodies. Production of site-specific antibodies to the T cell antigen receptor beta-chain. J Biol Chem 263:1719–1725PubMedGoogle Scholar
  48. Puccia R, Travassos LR (1991a) The 43-kDa glycoprotein from the human pathogen Paracoccidioides brasiliensis and its deglycosylated form: excretion and susceptibility to proteolysis. Arch Biochem Biophys 289: 298–302PubMedCrossRefGoogle Scholar
  49. Puccia R, Travassos LR (1991b) 43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo’s disease. J Clin Microbiol 29:1610–1615PubMedGoogle Scholar
  50. Puccia R, Schenkman S, Gorin PA, Travassos LR (1986) Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen. Infect Immun 53:199–206PubMedGoogle Scholar
  51. Puccia R, Takaoka DT, Travassos LR (1991) Purification of the 43 kDa glycoprotein from exocellular components excreted by Paracoccidioides brasiliensis in liquid culture (TOM medium). J Med Vet Mycol 29:57–60PubMedCrossRefGoogle Scholar
  52. Puccia R, Travassos LR, Rodrigues EG, Carmona AK, Oliveira MC, Juliano L (1994) Purification of the specific exocellular antigen gp43 from Paracoccidioides brasiliensis. Immunological and proteolytic activities. In: Maresca B, Kobayashi GS (eds) Molecular biology of fungi. Telos Press, New York, pp 507–515Google Scholar
  53. Puccia R, Carmona AK, Gesztesi JL, Juliano L, Travassos LR (1998) Exocellular proteolytic activity of Paracoccidioides brasiliensis: cleavage of components associated with the basement membrane. Med Mycol 36:345–348PubMedGoogle Scholar
  54. Puccia R, Juliano MA, Juliano L, Travassos LR, Carmona AK (1999) Detection of the basement membranedegrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp. Braz J Med Biol Res 32:645–649PubMedCrossRefGoogle Scholar
  55. Restrepo A, Moncada LH (1974) Characterization of the precipitin bands detected in the immunodiffusion test for paracoccidioidomycosis. Appl Microbiol 28: 138–144PubMedGoogle Scholar
  56. Rodrigues EG, Travassos LR (1994) Nature of the reactive epitopes in Paracoccidioides brasiliensis polysaccharide antigen. J Med Vet Mycol 32:77–81PubMedCrossRefGoogle Scholar
  57. Sano A, Defaveri J, Tanaka R, Yokoyama K, Kurita N, Franco M, Coelho KI, Bagagli E, Montenegro MR, Miyaji M, Nishimura K (1998) Pathogenicities and GP43 kDa gene of three Paracoccidioides brasiliensis isolates originated from a nine-banded armadillo (Dasypus novemcinctus). Mycopathologia 144:61–65PubMedCrossRefGoogle Scholar
  58. Saraiva EC, Altemani A, Franco MF, Unterkircher CS, Camargo ZP (1996) Paracoccidioides brasiliensis-gp43 used as paracoccidioidin. J Med Vet Mycol 34:155–161PubMedCrossRefGoogle Scholar
  59. Sercarz EE, Lehmann PV, Ametani A, Benichou G, Miller A, Moudgil K (1993) Dominance and crypticity of T cell antigenic determinants. Annu Rev Immunol 11: 729–766PubMedCrossRefGoogle Scholar
  60. Southwood S, Sidney J, Kondo A, del Guercio MF, Appella E, Hoffman S, Kubo RT, Chesnut RW, Grey HM, Sette A (1998) Several common HLA-DR types share largely overlapping peptide binding repertoires. J Immunol 160:3363–3373PubMedGoogle Scholar
  61. Souto JT, Figueiredo F, Furlanetto A, Pfeffer K, Rossi MA, Silva JS (2000) Interferon-gamma and tumor necrosis factor-alpha determine resistance to Paracoccidioides brasiliensis infection in mice. Am J Pathol 156: 1811–1820PubMedCrossRefGoogle Scholar
  62. Souza AR, Gesztesi JL, Moraes JZ, Cruz CR, Sato J, Mariano M, Lopes JD (1998) Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans. Clin Exp Immunol 114:40–48PubMedCrossRefGoogle Scholar
  63. Souza AR, Gesztesi JL, Del Negro GMB, Benard G, Sato J, Santos MVB, Abrahão TB, Lopes JD (2000) Antiidiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis. Clin Diag Lab Immunol 7:175–181Google Scholar
  64. Souza MC, Gesztesi JL, Souza AR, Moraes JZ, Lopes JD, Camargo ZP (1997) Differences in reactivity of paracoccidioidomycosis sera with gp43 isoforms. J Med Vet Mycol 35:13–18PubMedCrossRefGoogle Scholar
  65. Stambuk BU, Puccia R, de Almeida ML, Travassos LR, Schenkman S (1988) Secretion of the 43 kDa glycoprotein antigen by Paracoccidioides brasiliensis. J Med Vet Mycol 26:367–373PubMedCrossRefGoogle Scholar
  66. Straus AH, Freymuller E, Travassos LR, Takahashi HK (1996) Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies. J Med Vet Mycol 34:181–186PubMedCrossRefGoogle Scholar
  67. Sturniolo T, Bono E, Ding J, Raddrizzani L, Tuereci O, Sahin U, Braxenthaler M, Gallazzi F, Protti MP, Sinigaglia F, Hammer J (1999) Generation of tissue-specific and promiscuous HLA ligand databases using DNA microarrays and virtual HLA class II matrices. Nat Biotechnol 17:555–561PubMedCrossRefGoogle Scholar
  68. Taba MR, Silveira JF, Travassos LR, Schenkman S (1989) Expression in Escherichia coli of a gene for epitopes of a diagnostic antigen of Paracoccidioides brasiliensis: relationship with clinical forms of paracoccidioidomycosis. Exp Micol 13:223–230CrossRefGoogle Scholar
  69. Taborda CP, Camargo ZP (1993) Diagnosis of paracoccidioidomycosis by passive haemagglutination assay of antibody using a purified and specific antigen-gp43. J Med Vet Mycol 31:155–160PubMedCrossRefGoogle Scholar
  70. Taborda CP, Juliano MA, Puccia R, Franco M, Travassos LR (1998) Mapping of the T-cell epitope in the major 43kilodalton glycoprotein of Paracoccidioides brasiliensis which induces a Th-1 response protective against fungal infection in BALB/c mice. Infect Immun 66: 786–793PubMedGoogle Scholar
  71. Taborda CP, Franco MF, Reis LFL, Travassos LR (1999) Pathology of the intra-tracheal infection of Paracoccidioides brasiliensis in IFN-y, IFN-α-, ß3-, y-R and IRF-1 deficient mice. VII International Meeting on Paracoccidioidomycosis, April 29-May 2, E-26, p. 162; Campos do Jordâo, São Paulo, Brazil.Google Scholar
  72. Tapia FJ, Goihman-Yahr M, Caceres-Dittmar G, Altieri E, Gross A, Isturiz G, Rosquete R, Viloria N, Avila-Millan E, Carrasquero M (1991) Leukocyte immunophenotypes in bronchoalveolar lavage fluid and peripheral blood of paracoccidioidomycosis, sarcoidosis and silicosis. Histol Histopathol 6:395–402PubMedGoogle Scholar
  73. Travassos LR (1994) Immunochemistry of Paracoccidioides brasiliensis. In: Franco M, Lacaz CS, Restrepo-Moreno A, Del Negro G (eds) Paracoccidioidomycosis. CRC Press, Boca Raton, pp 67–86Google Scholar
  74. Travassos LR, Puccia R, Cisalpino P, Taborda C, Rodrigues EG, Rodrigues M, Silveira JF, Almeida IC (1995) Biochemistry and molecular biology of the main diagnostic antigen of Paracoccidioides brasiliensis. Arch Med Res 26:297–304PubMedGoogle Scholar
  75. Travassos LR, Nakaie CR, Cilli EM, Taborda CP (2000) Synthesis and immunological activity of a branched peptide construction carrying the T cell epitope of Paracoccidioides brasiliensis major exocellular antigen, the gp43. Anais Acad Bras Ciências 72: 5110–5114Google Scholar
  76. Tsuji M, Zavala F (2001) Peptide-based subunit vaccines against pre-erythrocytic stages of malaria parasites. Mol Immunol 38:433–442PubMedCrossRefGoogle Scholar
  77. Ulmer JB, Donnelly JJ, Parker SE, Rhodes GH, Felgner PL, Dwarki VJ, Gromkowski SH, Deck RR, DeWitt CM, Friedman A (1993) Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259:1745–1749.PubMedCrossRefGoogle Scholar
  78. Vicentini AP, Gesztesi JL, Franco MF, de Souza W, de Moraes JZ, Travassos LR, Lopes JD (1994) Binding of Paracoccidioides brasiliensis to laminin through surface glycoprotein gp43 leads to enhancement of fungal pathogenesis. Infect Immun 62:1465–1469PubMedGoogle Scholar
  79. Vicentini AP, Moraes JZ, Gesztesi JL, Franco MF, de Souza W, Lopes JD (1997) Laminin-binding epitope on gp43 from Paracoccidioides brasiliensis is recognized by a monoclonal antibody raised against Staphylococcus aureus laminin receptor. J Med Vet Mycol 35:37–43PubMedCrossRefGoogle Scholar
  80. Yarzabal L (1982) Composición antigénica de Paracoccidioides brasiliensis. In: Del Negro G, Lacaz CS, Fiorillo AM (eds) Paracoccidioidomicose. Sarvier-Edusp, São Paulo, pp 59–67Google Scholar
  81. Yarzabal LA, Andrieu S, Bout D, Naquira F (1976) Isolation of a specific antigen with alkaline phosphatase activity from soluble extracts of Paracoccidioides brasiliensis. Sabouraudia 14:275–280PubMedCrossRefGoogle Scholar
  82. Yarzabal LA, Bout D, Naquira F, Fruit J, Andrieu S (1977) Identification and purification of the specific antigen of Paracoccidioides brasiliensis responsible for immunoelectrophoretic band E. Sabouraudia 15:79–85PubMedCrossRefGoogle Scholar
  83. Yarzabal LA, De Albornoz MB, De Cabral NA, Santiago AR (1978) Specific double diffusion microtechnique for the diagnosis of aspergillosis and paracoccidioidomycosis using monospecific antisera. Sabouraudia 16:55–62PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Luiz R. Travassos
    • 1
  • Carlos P. Taborda
    • 2
  • Leo K. Iwai
    • 3
  • Ed Cio Cunha-Neto
    • 1
  • Rosana Puccia
    • 1
  1. 1.Departamento de Microbiologia, Imunologia e ParasitologiaUniversidade Federal de São Paulo (UNIFESP)Brazil
  2. 2.Departamento de MicrobiologiaUniversidade de São Paulo (USP)Brazil
  3. 3.Laboratório de ImunologiaINCOR, USPSão PauloBrazil

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