Biochemistry and Molecular Biology of Entomopathogenic Fungi

  • G. G. Khachatourians
Part of the The Mycota book series (MYCOTA, volume 6)


Fungal disease of insects represents an important facet of the interaction between entomopathogenic fungi (EPF) and their hosts. Altogether some 90 genera and 700 species are involved with entomopathogenicity, but only a few members of the entomophthorales and hyphomycetes have been well studied (Khachatourians 1991). The long-range potential of the EPF in research is their application in insect pest management or biocontrol (Leathers et al. 1993). Commercialization of EPF for pest control requires understanding of physiological aspects of growth, metabolic activity, genetic basis of virulence and host specificity-challenges that were forecast to be met with the new biotechnology (Khachatourians 1986; Boucias 1988). With such knowledge, physiological manipulations, isolation of mutants with enhanced virulence, and construction of environmentally safe strains with limited persistence should be possible within the forseeable future.


Entomopathogenic Fungus Beauveria Bassiana Cuticular Protein Insect Cuticle Hyphal Body 
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  1. Allee LL, Goettel MS, Gol’berg A, Whitney HS, Roberts DW (1990) Infection by Beauveria bassiana of Leptino-tarsa decemlineata larvae as a consequence of fecal contamination of the integument following per os inoculation. Mycopathologia 111:17–24Google Scholar
  2. Andersen SO, Hojrup P, Roepstorff P (1986) Characterization of cuticular proteins from the migratory locust, Locusta migratoria. Insect Biochem 16:441–447Google Scholar
  3. Andreadis TG, Weseloh RM (1990) Discovery of Entomophaga maimaiga in North American gypsy moth Lymantria dispar. Proc Nat Acad Sci USA 87:2461–2465PubMedGoogle Scholar
  4. Aregger E (1992) Conidia production of the fungus Beauveria brongniartii on barley and quality evaluation during storage at 2°C. J Invertbr Pathol 59:2–10Google Scholar
  5. Bartlet MC, Jaronski (1988) Mass production of entomogenous fungi for biological control of insects. Chap 4. In: Burgess RN (ed) Fungi in biological control systems. Manchester University Press, Manchester, pp 61–85Google Scholar
  6. Berbee ML, Kerwin JL (1993) Ultrastructural and light microscopic localization of carbohydrates and peroxi-dase/catalase in Lagenidium giganteum zoospores. Mycologia 85:734–743Google Scholar
  7. Bidochka MJ, Khachatourians GG (1987) Hemocytic defense response to the entomopathogenic fungus Beauveria bassiana in the migratory grasshopper Melanoplus sanguinipes Entomol Exp Appl 45:151–156Google Scholar
  8. Bidochka MJ, Khachatourians GG (1988a) Regulation of extracellular protease in the entomopathogenic fungus Beauveria bassiana. Exp Mycol 12:161–168Google Scholar
  9. Bidochka MJ, Khachatourians GG (1988b) N-acetyl-d-glucosamine-mediated regulation of extracellular protease in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 54:2699–2704PubMedGoogle Scholar
  10. Bidochka MJ, Khachatourians GG (1990) Identification of Beauveria bassiana extracellular protease as a virulence factor in pathogenicity toward the migratory grasshopper, Melanoplus sanguinipes. J Invertebr Pathol 56: 362–370Google Scholar
  11. Bidochka MJ, Khachatourians GG (1991) The implication of metabolic acids produced by Beauveria bassiana in pathogenesis of the migratory grasshopper, Melanoplus sanguinipes. J Insect Pathol 58:106–117Google Scholar
  12. Bidochka MJ, Khachatourians GG (1992) Growth of the entomopathogenic fungus Beauveria bassiana on cuticular components from the migratory grasshopper Melanoplus sanguinipes. J Invertbr Pathol 59:165–173Google Scholar
  13. Bidochka MJ, Khachatourians GG (1993a) Regulation of extracellular N-acetyl-d-glucosaminidase in the entomopathogenic fungus Beauveria bassiana. Can J Microbiol 39:6–12PubMedGoogle Scholar
  14. Biodochka MJ, Khachatourians GG (1993b) Oxalic acid overproduction in Beauveria bassiana mutants is related to a utilizable carbon source but not virulence. J Invertebr Pathol 62:53–57Google Scholar
  15. Bidochka MJ, Khachatourians GG (1994a) Protein hydrolysis in grasshoppers by entomopathogenic fungal proteases. J Invertbr Pathol 63:7–13Google Scholar
  16. Bidochka MJ, Khachatourians GG (1994b) Effect of cuticular modification on their degradation by entomopathogenic fungal extracellular proteases. J Invertbr Pathol 64:26–32Google Scholar
  17. Bidochka MJ, Low NH, Khachatourians GG (1990) Storage carbohydrates of the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 50: 3186–3190Google Scholar
  18. Bidochka MJ, Tong KI, Khachatourians GG (1993) Purification, properties of two extracellular N-acetyl-d-glucosaminidases produced by the entomopathogenic fungus Beauveria bassiana. Can J Microbiol 39:40–45PubMedGoogle Scholar
  19. Bidochka MJ, McDonald MA, St Leger RJ, Roberts DW (1994) Differentiation of species and strains of entomopathogenic fungi by random amplification of polymorphic DNA (RAPD). Curr Genet 25:107–113PubMedGoogle Scholar
  20. Billich A, Zocher R (1988) Constitutive expression of enniatin synthetase during fermentative growth of Fusarium scirpi. Appl Environ Microbiol 54:2504–2509PubMedGoogle Scholar
  21. Bing LA, Lewis LC (1992) Temporal relationship between Zea mays, Ostrinia nubilalis (Lep : Pyralidae) and endophytic, Beauveria bassiana. Entomophaga 37:525–536Google Scholar
  22. Boucias DG (1988) Genetic improvement of entomopathogenic fungi. In: Herzog GA, Ramaswamy S, Lentz G, Goodenough JL, Hamm JJ (eds) Theory and tactics of Heliothis population management, III Emerging control tactics and techniques. South Coop Ser Bull 337:66–68Google Scholar
  23. Boucias DG, Pendland JC (1982) Ultrastructural studies on the fungus, Nomuraea rileyi, infecting the velvetbean caterpillar, Anticarsia gemmatalis. J Invertebr Pathol 39:338–345Google Scholar
  24. Boucias DG, Pendland JC (1987) Detection of protease inhibitors in the hemolymph of resistant Anticarsia gemmatalis which are inhibitory to the entomopathogenic fungus, Nomuraea rileyi. Experientia 43:336–339Google Scholar
  25. Boucias DG, Pendland JC (1991a) The fungal cell wall and its involvement in the pathogenic process in insect hosts. In: Latge JP, Boucias DG (eds) Fungal cell wall and immune response. Springer Berlin Heidelberg New York, pp 121–137Google Scholar
  26. Boucias DG, Pendland JC (1991b) Attachment of mycopathogens to cuticle: The initial event of mycosis in arthropod hosts. In: Cole GT, Hoch HC (eds) The fungal spore and disease initiation in plants and animals. Plenum Press, New York, pp 101–128Google Scholar
  27. Boucias DG, Pendland JC (1993a) The galactose binding lectin from the beet armyworm, Sprodoptera exigua description and site of synthesis. Insect Biochem Mol Biol 23:233–242PubMedGoogle Scholar
  28. Boucias DG, Pendland JC (1993b) Variability in the ability of galactose and mannose specific lectins to bind to cell wall surfaces during growth of the insect pathogenic fungus Paecilomyces farinosus. Eur J Cell Biol 60: 322–330PubMedGoogle Scholar
  29. Boucias DG, Bradford DL, Barfield CS (1984) Susceptibility of the velvetbean caterpillar and soybean looper (Lepidoptera: Noctuidae) to Nomuraea rileyi: effects of pathotype, dosage, temperature, and host age. J Econ Entomol 77:247–253Google Scholar
  30. Boucias DG, Pendland JC, Latge JP (1988) Nonspecific factors involved in attachment of entomopathogenic Deuteromycetes to host insect cuticle. Appl Environ Microbiol 54:1795–1805PubMedGoogle Scholar
  31. Breen JP (1993) Enhanced resistance to fall armyworm Lepidoptera nucutidae in acremonium endophyte-infected turfgrasses. J Econ Entomol 86:621–629Google Scholar
  32. Bridge PD, Williams MAJ, Prior C, Paterson RRM (1993) Morphological, biochemical and molecular characteristics of Metarhizium anisopliae and M. flavoviride. J Gen Microbiol 139:1163–1169Google Scholar
  33. Brobyn PJ, Wilding N (1983) Invasive and developmental process of Entomophthora muscae infecting house flies Musca domestica. Trans Br Mycol Soc 80:1–8Google Scholar
  34. Brody H, Griffith J, Cuticchia AJ, Arnold J, Timberlake WE (1991) Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans. Nucleic Acids Res 19:3105–3109PubMedGoogle Scholar
  35. Calhoun LA, Findlay JA, Miller JD, Whitney NJ (1993) Metabolites toxic to spruce budworm from balsam fir needle endophytes. Mycol Res 96:281–286Google Scholar
  36. Carruthers RI, Soper RS (1987) Fungal diseases. In: Fuxa JR, Tanada Y (eds) Epizootiology of insect diseases. Wiley-Interscience, New York, pp 357–416Google Scholar
  37. Carruthers RI, Larkin TS, Firstencel H (1992) Influence of thermal ecology on the mycosis of rangeland grasshoppers. Ecology 73:190–240Google Scholar
  38. Cerenius L, Thornqvist P-O, Vey A, Johansson MW, Soderhall KC (1990) The effect of the fungal toxin destruxin E on isolated crayfish hemocytes. J Insect Physiol 36:785–789Google Scholar
  39. Charnley AK, St Leger RJ (1991) The role of cuticle degrading enzymes in fungal pathogenesis in insects. In: Cole GT, Hoch HC (eds) The fungal spore and disease initiation in plants and animals. Plenum Press New York, pp 267–286Google Scholar
  40. Cherton J-C, Loutelier C, Langer L, Cassier P (1993) Direct injection onto the HPLC column for monitoring the behavior of xenobiotics in biological media II. Behavior of cymoxanil in fungus and locust and of E destruxin in locust. Sci Total Environ 132:381–397PubMedGoogle Scholar
  41. Cheung PYK, Grula EA (1982) In vivo events associated with entomopathology of Beauveria bassiana for the corn earworm (Heliothis zea). J Invertbr Pathol 39: 303–313Google Scholar
  42. Clay K (1988) Fungal endophytes of grasses: a defensive mutualism between plants and fungi. Ecology 69:10–16Google Scholar
  43. Cole SCJ, Charnley AK, Cooper RM (1993) Purification and partial characterization of a novel trypsin like cysteine protease from Metarhizium anisopliae. FEMS Microbiol Lett 113:189–195Google Scholar
  44. Costa SD, Gaugier R (1990) Sensitivity of Beauveria bassiana to solanine and tomatine: plant defensive chemicals inhibit an insect pathogen. J Chem Ecol 15:697–706Google Scholar
  45. Dean DD, Domnas AJ (1983) The extracellular enzymes of the mosquito-parasitizing fungus Lagenidium giganteum. Exp Mycol 7:31–39Google Scholar
  46. Desgranges C, Vergoignan C, Lereec A, Riba G, Durand A (1993) Use of solid state fermentation to produce Beauveria bassiana for the biological control of European corn borer. Biotechnol Adv 11:577–587PubMedGoogle Scholar
  47. Dillon RJ, Charnley AK (1991) The fate of fungal spores in the insect gut. In: Cole GT, Hoch HC (eds) The fungal spore and disease initiation in plants and animals. Plenum Press, New York, pp 129–156Google Scholar
  48. Domnas AJ, Warner SA (1991) Biochemical activities of entomogenous fungi. Crit Rev Microbiol 18:1–13PubMedGoogle Scholar
  49. Dorschner KW, Feng M-G, Baird CR (1991) Virulence of an aphid-derived isolate of Beauveria bassiana (Fungi : Hyphomycetes) to hop aphid, Phorodon humuli (Homoptera: Aphididiae). Environ Entomol 20:690–693Google Scholar
  50. Dowd P (1988) Synergism of aflatoxin Bl toxicity with the co-occurring fungal metabolite kojic acid to two caterpillars. Entomol Exp Appl 47:69–71Google Scholar
  51. Dowd P (1992) Detoxification of mycotoxins by insects. In: Mullin CA, Scott JG (eds) Molecular mechanisms of insecticide resistance: diversity among insects. American Chem Soc Press Washington DC, pp 264–275Google Scholar
  52. Drif L, Brehlin M (1989) Agglutinin-mediated immune recognition in Locusta migratoria (Insecta). J Insect Physiol 35:729–736Google Scholar
  53. Drummond J, Heale JB (1988) Genetic studies on the inheritance of pathogenicity in Verticillium lecanii against Trialeurodes vaporariorum. J Invertebr Pathol 52:57–65Google Scholar
  54. Egger KN, Danielson RM, Fortin JA (1991) Taxonomy and population structure of E-strain mycorrhizial fungi inferred from ribosomal and mitochondrial DNA polymorphisms. Mycol Res 95:866–872Google Scholar
  55. El-Sayed GN, Ignoffo CM, Leathers TD, Gupta SC (1992) Use of a colorimetric system to detect enzymes expressed by germinating conidia of entomopathogenic fungi. Mycopathologia 118:29–36PubMedGoogle Scholar
  56. El-Sayed GN, Ignoffo CM, Leathers TD, Gupta SC (1993a) Cuticular and non-cuticular substrate influence on expression of cuticle degrading enzymes from conidia of an entomopathogenic fungus Nomurea rileyi. Mycopathologia 122:79–87Google Scholar
  57. El-Sayed GN, Ignoffo CM, Leathers TD, Gupta SC (1993b) Insect cuticle and yeast extract effects on germination growth and production of hydrolytic enzymes of Normurea rileyi. Mycopathologia 122:143–147Google Scholar
  58. El-Sayed GN, Ignoffo CM, Leathers TD, Gupta SC (1993c) Effects of cuticle and concentration on expression of hydrolytic enzymes by an entomopathogenic fungus Nomurea rileyi. Mycopathologia 122:149–152Google Scholar
  59. Fargues J, Remaudiere G (1977) Considerations on the specificity of entomopathogenic fungi. Mycopathologia 62:31–37Google Scholar
  60. Fargues J, Delmas JC, Lebrun RA (1994) Leaf consumption by larvae of the Colorado potato beetle (Coleoptera : Chrysomelidae) infected with the entomo-pathogen, Beauveria bassiana. J Econ Entomol 87: 67–71Google Scholar
  61. Fegan M, Manners JM, Maclean DJ, Irwin JAG, Samuels KDZ, Holdom DE, Li DP (1993) Random amplified polymorphic DNA markers reveal a high degree of genetic diversity in the entomopathogenic fungus Metar-hizium anisopliae var. anisopliae. J Gen Microbiol 139: 2075–2081PubMedGoogle Scholar
  62. Feng M-G, Johnson JB (1990) Relative virulence of six isolates of Beauveria bassiana on Diuraphis noxia (Homoptera : Aphididae). Environ Entomol 19:785–790Google Scholar
  63. Feng M-G, Poprawski TJ, Khachatourians GG (1994) Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control. Biocontrol Sci Technol 4:3–34Google Scholar
  64. Ferron P, Fargues J, Riba G (1991) Fungi as microbial insecticides against pests. In: Arora DK, Mukerji KG, Drouhet E (eds) Handbook of applied mycology: humans, animals and insects. Marcel Dekker, New York, pp 665–706Google Scholar
  65. Funk CJ, Ramoska WA, Bechtel DB (1993) Histopathology of Entomophaga grylii pathotype II infections in Melanoplus differentialis. J Invertebr Pathol 61:196–202Google Scholar
  66. Fuxa JR (1991) Release and transport of entomopathogenic microorganisms. In: Morris LA, Strauss HS (eds) risk assessment in genetic engineering. McGraw Hill, New York, pp 83–113Google Scholar
  67. Gallardo F, Borethel DJ, Fuxa JR, Richter A (1990) Susceptability of Heliothis zea (Boddie) larvae to Nomurea rileyi (Farlow) Samson: effects of alpha tomatine at the third trophic level. J Chem Ecol 16: 1751–1759Google Scholar
  68. Gillespie JP, Khachatourians GG (1992) Characterization of the Melanoplus sanguinipes, hemolymph after infection with Beauveria bassiana or wounding. Comp Biochem Physiol 103B:455–463Google Scholar
  69. Gillespie JP, Bidochka MJ, Khachatourians GG (1991) Separation and characterization of hemolymph phenoloxidase by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comp Biochem Physiol 98C:351–358Google Scholar
  70. Gilliam M., Lorenz BJ, Prest DB, Camazine S (1993) Ascosphaera apis from Apis cerana from South Korea. J Invertbr Pathol 61:111–112Google Scholar
  71. Glare TR, Jackson TA (eds) (1992) Use of pathogens in scarab pest management. Intercept Andover England, 298 ppGoogle Scholar
  72. Glare TR, Milner RJ (1991) Ecology of entomopathogenic fungi. In: Arora DK, Mukerji KG, Drouhet E (eds) Handbook of applied mycology: humans, animals and insects. Marcel Dekker, New York, pp 547–612Google Scholar
  73. Gobb BD, Clarkson JM (1993) Detection of molecular variation in the insect pathogenic Metarhizium anisopliae using RAPD-PCR. FEMS Microbiol Lett 112:319–324Google Scholar
  74. Godoy G, Steadman JR, Dickman, MB, Dam R (1990) Use of mutants to demonstrate the role of oxalic acid in pathogenicity of Sclerotinia sclerotiorum on Phaseolus vulgaris. Physiol Mol Plant Pathol 37:179–191Google Scholar
  75. Goerzen DW, Erlandson MA, Moore KC (1990) Effect of two insect viruses and two entomopathogenic fungi on larval and pupal development in the alfalfa leafcutting bee, Megachile rotundata (Fab.) (Hymenoptera : Megachilidae). Can Entomol 122:1039–1040Google Scholar
  76. Golkar L, LeBrun RA, Ohayon H, Gounon P, Papierok B, Brey TB (1993) Variation of larval susceptibility to Lagenidium giganteum in three mosquito species. J Invertebr Pathol 62:1–8PubMedGoogle Scholar
  77. Gunnarson S (1987) Cellular immune reactions in the desert locust Schistocerca gregaria infected by the fungus Metarhizium anisopliae. PhD Thesis, Uppsala University, UppsalaGoogle Scholar
  78. Gupta SC, Leathers TD, El-Sayed GN, Ignoffo CM (1991) Production of degradative enzymes by Metarhizium anisopliae during growth on defined media and insect cuticle. Exp Mycol 15:310–315Google Scholar
  79. Gupta SC, Leathers TD, El-Sayed GN, Ignoffo CM (1992) Insect degrading enzymes from entomogenous fungus Beauveria bassiana. Exp Mycol 16:132–137Google Scholar
  80. Guy LP, Rath CA (1990) Enzyme linked immunoabsor-bent assays (ELISA) to detect spore surface antigens of Metarhizium anisopliae. J Invertebr Pathol 55:435–436Google Scholar
  81. Hajek AE, Soper RS (1992) Temporal dynamics of Entomophaga maimaiga after death of Gypsy moth (Lepidoptera : Lymantriidae) larval hosts. Environ Entomol 21:129–135Google Scholar
  82. Hajek AE, St Leger RJ (1994) Interactions between fungal pathogens and insect hosts. Annu Rev Entomol 39: 293–322Google Scholar
  83. Hajek AE, Humber RA, Elkinton JS, May B, Walsh SRA, Silver JC (1990) Allozyme and RFLP analyses confirm Entomophaga maimaiga responsible for 1989 epizootics in North American Gypsy moth populations. Proc Natl Acad Sci USA 87:6979–6982PubMedGoogle Scholar
  84. Hajek AE, Humber RA, Walsh SRA, Silver JC (1991) Sympatric occurrence of two Entomophaga aulicae (Zygomycetes : Entomophthorales) complex species attacking forest Lepidoptera. J Inverbr Pathol 58:373–380Google Scholar
  85. Hallsworth JE, Magan N (1994a) Effects of KCl concentration on accumulation of acyclic sugar alcohols and trehalose in conidia of three entomopathogenic fungi. Lett Appl Microbiol 18:8–11Google Scholar
  86. Hallsworth JE, Magan N (1994b) Effects of carbohydrate type and concentration on polyhydroxy alcohol and trehalose content of conidia of three entomopathogenic fungi. Microbiol UK 140:2705–2713Google Scholar
  87. Hallsworth JE, Magan N (1995) Manipulation of intracellular glycerol and erythritol enhances germination of conidia at low water availability. Microbiol UK 145: 1109–1115Google Scholar
  88. Hassan AEM, Charnley AK (1989) Ultrastructural study of the penetration by Metarhizium anisopliae through the Dimilin-affected cuticle of Manduca sexta. J Invertbr Pathol 54:117–124Google Scholar
  89. Hassan AEM, Dillon RJ, Charnley AK (1989) Influence of accelerated germination of conidia on the pathogenicity of Metarhizium anisopliae for Manduca sexta. J Invertebr Pathol 54:277–279Google Scholar
  90. Havukkala I, Mitamuta C, Hara S, Hirayae K, Nishizawa Y, Hibi T (1993) Induction and purification of Beauveria bassiana chitinolytic enzymes. J Invertebr Pathol 61: 97–102Google Scholar
  91. Hayden T, Bidochka MJ, Khachatourians GG (1992) Virulence of several entomopathogenic fungi and host-passage strains of Paecilomyces farinosus toward the Blackberry-Cereal Aphid Sitobion fragariae. J Econ Entomol 85:58–64Google Scholar
  92. Hegedus DD, Khachatourians GG (1993a) Construction of cloned DNA probes for the specific detection of the entomopathogenic fungus Beauveria bassiana in grasshoppers. J Invertebr Pathol 62:233–240Google Scholar
  93. Hegedus DD, Khachatourians GG (1993b) Identification of molecular variants in mitochondrial DNAs of members of the genera Beauveria, Verticillium, Paecilomyces, Tolypocladium and Metarhizium. Appl Environ Microbiol 59:4283–4288PubMedGoogle Scholar
  94. Hegedus DD, Khachatourians GG (1994) Production and characterization of conditional lethal mutants of Beauveria bassiana. Can J Microbiol 40:766–776Google Scholar
  95. Hegedus DD, Khachatourians GG (1995a) Polymerase chain reaction and single-strand conformation polymorphism analysis of the entomopathogenic fungus Beauveria bassiana. J Invertbr Pathol (submitted)Google Scholar
  96. Hegedus DD, Khachatourians GG (1995b) The effects of temperature on the pathogenicity of heat-sensitive mutants of the entomopathogenic fungus, Beauveria bassiana, toward the migratory grasshopper, Melanoplus sanguinipes. J Invertbr Pathol (submitted)Google Scholar
  97. Hegedus DD, Khachatourians GG (1995c) Analysis of cellular defense reactions of the migratory grasshopper, Melanoplus sanguinipes, infected with heat-sensitive mutants of Beauveria bassiana. J Invertbr Pathol (submitted)Google Scholar
  98. Hegedus DD, Bidochka MJ, Khachatourians GG (1990) Beauveria bassiana submerged conidia production in a defined medium containing chitin, two hexosamines or glucose. Appl Microbiol Biotechnol 33:641–647Google Scholar
  99. Hegedus DD, MacPherson JM, Pfeifer TA, Khachatourians GG (1991) DNA sequence of tRNAVal-tRNAIle tandem genes from the mitochondria of the entomopathogenic fungus Beauveria bassiana GK2016. Gene 109:149–154PubMedGoogle Scholar
  100. Hegedus DD, Bidochka MJ, Miranpuri GS, Khachatourians GG (1992) A comparison of the virulence, stability and cell-wall surface characteristics of three spore types produced by the entomopathogenic fungus Beauveria bassiana. Appl Microbiol Biotechnol 36:785–789Google Scholar
  101. Hokkanen HMT (1993) Overwintering survival and spring emergence in Meligethes aeneus: effects of body weight, crowding and soil treatment with Beauveria bassiana. Entomol Exp Appl 67:241–246Google Scholar
  102. Hsiao WF, Bidochka MJ, Khachatourians GG (1992a) Effects of diphenols on the growth of three entomopathogenic fungi. Can J Microbiol 38:1000–1003Google Scholar
  103. Hsiao WF, Bidochka MJ, Khachatourians GG (1992b) Effect of temperature, relative humidity on the virulence of the entomopathogenic fungus Verticillium lecanii toward the oat-bird berry aphid Rhopalosiphum padi. (Homoptera: Aphidiae). J Appl Entomol 114:484–490Google Scholar
  104. Hung S-Y, Boucias DG (1992) Influence of Beauveria bassiana on the cellular defense response of the beet armyworm, Spodoptera exigua. J Invertbr Pathol 60: 152–158Google Scholar
  105. Hung S-Y, Boucias DG, Vey AJ (1993) Effect of Beauveria bassiana and Candida albicans on the cellular defense of Spodoptera exigua. J Invertebr Pathol 61:179–187PubMedGoogle Scholar
  106. Huxham IM, Samuels KDZ, Heale JB, Lackey AM, McCorkindale NJ (1988) In vivo and in vitro assays for pathogenicity of wild-type and mutant strains of Metarhizium anisopliae for three insect species. J Invertebr Pathol 53:143–151Google Scholar
  107. Huxham IM, Lackey AM, McCorkindale NJ (1989) Inhibitory effects of cyclodepsipeptides, destruxins, from the fungus Metarhizium anisopliae on cellular immunity in insects. J Insect Physiol 35:97–105Google Scholar
  108. Iijima R, Kurata S, Natori S (1993) Purification, characterization and cDNA cloning of an antifungal protein from the hemolymph of Sarcophaga peregrina (flesh fly) larvae. J Biol Chem 268:12055–12061PubMedGoogle Scholar
  109. James PJ, Kershaw MJ, Reynolds SE, Charnley AK (1993) Inhibition of desert locust (Schistocerca gregaria) Malpighian tubule fluid secretion by destruxins, cyclic peptide toxins from the insect pathogenic fungus Metarhizium anisopliae. J Insect Physiol 39:797–804Google Scholar
  110. Jansson HB (1993) Adhesion to nematodes of conidia from the nematophagous fungus Drechmeria consiospora. J Gen Microbiol 139:1899–1906Google Scholar
  111. Jegorov A, Sedmera P, Matha V (1993a) Biosynthesis of destruxins. Phytochemistry 33:1403–1405PubMedGoogle Scholar
  112. Jegorov A, Matha V, Husak M, Kratochvil B, Stuchlik J, Sedmera P, Havlicek V (1993b) Iron uptake system of some members of the genus Tolypocladium: crystal structure of the ligand and its iron (III) complex. J Chem Soc Dalton Trans 1:1287–1293Google Scholar
  113. Jeng RS, Duchesne LC, Sabourin M, Hubbes M (1991) Mitochondrial DNA restriction fragment length polymorphisms of aggressive and non-aggressive isolates of Ophiostoma ulmi. Mycol Res 95:537–542Google Scholar
  114. Jenkins NE, Prior C (1994) Growth and formation of true conidia by Metarhizium flavoviride in a simple liquid medium. Mycol Res 97:1489–1494Google Scholar
  115. Joshi L, Charnley AK, Arnold G, Brain P, Bateman R (1992) Synergism between entomopathogenic fungi Metarhizium spp. and the benzoylphenyl urea insecticide teflubenzuron against the desert locust Schistocerca gregaria. In: Pests and Diseases British Crop Protection Council, Farnham England, pp 369–374Google Scholar
  116. Joshi L, St Leger RJ, Bidochka MJ (1995) Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana. FEMS Microbiol Lett 125: 211–218PubMedGoogle Scholar
  117. Keller S (1991) Arthropod-pathogenic Entomopthorales of Switzerland. II. Erynia, Eryniopsis, Neozygites, Zoopteora and Tarichium. Sydowia 43:39–122Google Scholar
  118. Kerwin JL (1984) Fatty acid regulation of the germination of Erynia variabilis conidia on adults and puparia of the lesser housefly, Fannia canicularis. Can J Microbiol 38:158–161Google Scholar
  119. Kerwin JL, Washino RK (1986) Oosporogenesis by Lagenidium giganteum: induction and maturation are regulated by calcium and calmodulin. Can J Microbiol 32:663–672PubMedGoogle Scholar
  120. Kerwin JL, Simmons CA, Washion RK (1986) Oosporogenesis by Lagenidium giganteum in liquid culture. J Invertebr Pathol 47:258–270Google Scholar
  121. Khachatourians GG (1986) Production and use of biological pest control agents. Trends Biotechnol 4:120–124Google Scholar
  122. Khachatourians GG (1991) Physiology and genetics of entomopathogenic fungi. In: Arora DK, Ajello L, Mukerji KG (eds) Handbook of applied mycology, vol 2: Humans, animals, and insects. Marcel Dekker, New York, pp 613–661Google Scholar
  123. Khachatourians GG (1992) Virulence of five Beauveria strains, Paecilomyces farinosus and Verticillium lecanii against the migratory grasshopper Melanoplus sanguinipes. J Invertebr Pathol 59:212–214Google Scholar
  124. Koshinsky HA, Khachatourians GG (1995) Cloning, RFLP and karyotyping. In: Hui YH, Khachatourians GG (eds) Food biotechnology: microorganisms, VCH Publishers, New York, pp 85–132Google Scholar
  125. Kosir JM, MacPherson JM, Khachatourians GG (1991) Genomic analysis of virulent and less virulent strain of the entomopathogenic fungus Beauveria bassiana using RFLP. Can J Microbiol 37:534–541PubMedGoogle Scholar
  126. Kotlyarevskii DI, Levites EV (1992) Use of isoenzymes to detect parasexual process in the fungus Verticillium lecanii (Zim) Viegas. Doklady: Biol Sci 319:427–428Google Scholar
  127. Krasnoff SB, Gupta S (1991) Identification and biosynthesis of efrapeptins in fungus Tolycladium geodes Gams (Deuteromycotina:Hyphmycetes). J Chem Ecol 17: 1953–1960Google Scholar
  128. Krasnoff SB, Gupta SB, St Leger RJ, Renwick JAA, Roberts DW (1991) Antifungal and insecticidal properties of efraptins: metabolites of the fungus Tolypocladium niveium. J Invertbr Pathol 58:180–188Google Scholar
  129. Kucera M (1980) Proteases from the fungus Metarhizium anisopliae toxic for Galleria mellonella larvae. J Invertebr Pathol 35:37–48Google Scholar
  130. Kucera M (1981) The production of toxic protease by the entomopathogenous fungus Metarhizium anisopliae in submerged culture. J Invertebr Pathol 38:33–38Google Scholar
  131. Lane BS, Trinci AP, Gillespie AT (1991a) Endogenous reserves and survival of blastospores of Beauveria bassiana harvested from carbon and nitrogen limited batch cultures. Mycol Res 95:821–828Google Scholar
  132. Lane BS, Trinci AP, Gillespie AT (1991b) Influence of cultural conditions on the virulence of conidia and of blastospores of Beauveria bassiana to the green leafhopper Nephotettix virescens. Mycol Res 95:829–833Google Scholar
  133. Latge J, Monsigny M, Prevost M (1988) Visualization of exocellular lectins in the entomopathogenic fungus Conidiobolus obscurus. J Histochem Cytochem 36: 1419–1424PubMedGoogle Scholar
  134. Leathers TD, Gupta SC, Alexander NJ (1993) Mycopes-ticides: status, challenges and potential. J Ind Microbiol 12:69–75Google Scholar
  135. Lecuona R, Riba G, Cassier P, Clement JL (1991) Alterations of insect epicuticular hydrocarbons during infection with Beauveria bassiana or Beauveria brongniartii. J Invertebr Pathol 58:10–18Google Scholar
  136. Lewis GC, White JF Jr, Bonnefont J (1993) Evaluation of grasses infected with fungal endophytes against locusts. Ann Appl Biol 122(Suppl): 142–143Google Scholar
  137. Lopez-Lastra CC, Boucias DG (1994) Studies on the cellular reactions of Spodoptera exigua larvae infected with the fungus Nomuraea rileyi. J Invertbr Pathol 63:101–102Google Scholar
  138. Lucarotti CJ (1992) Invasion of Aedes aegypti ovaries by Coelomomyces strogomyiae. J Invertebr Pathol 60: 176–184Google Scholar
  139. MacPherson JM, Khachatourians GG (1991a) Partial purification, characterization of β-galactosidase produced by Beauveria bassiana. Biotechnol Appl Biochem 13:217–230Google Scholar
  140. MacPherson JM, Khachatourians GG (1991b) Production of β-galactosidase in liquid cultures of Beauveria bassiana. Food Biotechnol 5:33–44Google Scholar
  141. Majchrowicz I, Poprawski TJ, Robert P-H, Maniania NK (1990) Effects of entomopathogenic and opportunistic fungi on Delia antiqua (Diptera : Anthomyiidae) at low relative humidity. Environ Entomol 19:1163–1167Google Scholar
  142. Masel AM, Irwin JAG, Manners JM (1993) Mini-chromosomes of Colletotrichum spp. infecting several host species in various countries. Mycol Res 97:852–856Google Scholar
  143. Mazet I, Huang S-Y, Boucias DG (1994) Detection of toxic metabolites in the hemolymph of Beauveria bassiana infected Spodoptera exigua larvae. Experientia 50: 142–147Google Scholar
  144. McDonald DM, Nolan RA (1995) Effects of relative humidity and temperature on Entomophaga aulicae conidium discharge from infected eastern Hemlock looper larvae and subsequent conidium development. J Invertebr Pathol 65:83–90Google Scholar
  145. McDowell JM, Funderburk JE, Boucias DG, Gilreath ME, Lynch RE (1990) Biological activity of Beauveria bassiana against Elsmopalpus lignosellus (Lepidoptera : Pyralidae) on leaf substrates and soil. Environ Entomol 19:137–141Google Scholar
  146. Mead LJ, Khachatourians GG, Jones GA (1988) Microbial ecology of the gut in laboratory stocks of the migratory grasshopper Melanoplus sanguinipes (Fab) (Ortho-ptera: Acrididae). Appl Environ Microbiol 54:1174–1181PubMedGoogle Scholar
  147. Miao VP, Covert SF, VanEtten H (1991) A fungal gene for antibiotic resistance on a dispensable (“B”) chromosome. Science 254:1773–1776PubMedGoogle Scholar
  148. Miller JD (1986) Toxic metabolites of epiphytic and endophytic fungi of conifer needles. In: Fokkema NJ, Var Huevel J (eds) Proc 4th Int Microbiol Phyllosphere. Cambridge University Press, Cambridge, pp 223–231Google Scholar
  149. Miller JD, Strongman D, Whitney NJ (1985) Observations on fungi associated with spruce budworm infested balsam fir needles. Can J For Res 15:896–901Google Scholar
  150. Milne R, Wright T, Welton M, Budau C, Gringorten L, Tyrell D (1992) Identification and partial purification of a cell lytic factor from Entomophaga aulicae. 25th Annu Meet Soc Invertbr Pathol, Heidelberg, Germany, Aug 16–21 (Abstr), pp 230Google Scholar
  151. Miranpuri GS, Khachatourians GG (1990) Larvicidal activity of blastospores and conidiospores of Beauveria bassiana (strain GK 2016) against age groups of Aedes aegypti. Vet Parasitol 37:155–162PubMedGoogle Scholar
  152. Miranpuri GS, Khachatourians GG (1991) Infection foci of entomopathogenic fungus Beauveria bassiana (strain GK 2016) in Aedes aegypti larvae. Acta Entomol Appl 59:19–27Google Scholar
  153. Miranpuri GS, Khachatourians GG (1993) Hemocyte surface changes in the migratory grasshopper, Melanoplus sanguinipes in response to wounding and infection with Beauveria bassiana. Entomol Exp Appl 68:157–164Google Scholar
  154. Miranpuri GS, Khachatourians GG (1994) Bacterial and fungal control of hematophagous vectors: A review. J Insect Sci 6:1–14Google Scholar
  155. Miranpuri GS, Bidochka MJ, Khachatourians GG (1991) Morphology and cytochemistry of hemocytes and analysis of hemolymph from Melanoplus sanguinipes (Ortho-ptera: Acrididae). J Econ Entomol 84:371–378Google Scholar
  156. Mollier P, Lagnel J, Fournet B, Aïoun A, Riba G (1994a) A glycoprotein highly toxic for Galleria mellonella larvae secreted by the entomopathogenic fungus Beauveria sulferescens. J Invertebr Pathol 64:200–207Google Scholar
  157. Mollier P, Lagnel J, Quiot JM, Aïoun A, Riba G (1994b) Cytotoxic activity in culture filtrates from the entomopathogenic fungus Beauveria sulferescens. J Invertebr Pathol 64:208–213Google Scholar
  158. Moore D, Prior C (1993) The potential of mycoinsecticides. Biocontrol News Info 14:31N–40NGoogle Scholar
  159. Moore D, Reed M, Le Patourel G, Abraham YJ, Prior C (1992) Reduction of feeding by the desert locust, Schistocerca gregaria after infection with Metarhizium flavoviride. J Invertebr Pathol 60:304–307Google Scholar
  160. Moorehouse ER, Gillespie AT, Charnley AK (1993) Laboratory selection of Metarhizium spp. isolated for control of vine weevil larvae (Otiorhynchus sulcatus). J Invertebr Pathol 62:15–21Google Scholar
  161. Morgan LW, Boddy L, Clark SJ, Wilding N (1995) Influence of temperature on germination of primary and secondary conidia of Ergnia neoaphidis (Zygomycetes : Entomophtorales). J Invertbr Pathol 65:132–138Google Scholar
  162. Mugnai L, Bridge PD, Evans HC (1989) A chemotaxonomic evaluation of the genus Beauveria. Mycol Res 92:199–209Google Scholar
  163. Mule G, D’Ambrosio A, Logrieco A, Bottalico A (1993) Toxicity of mycotoxins of Fusarium sambucinum for feeding in Galleria mellonella. Entomol Exp Appl 62: 17–22Google Scholar
  164. Murrin F, Holtby J, Nolan RA, Davidson WS (1986) The genome of Entomophaga aulicae (Entomophthorales, Zygomycetes): base composition and size. Exp Mycol 10:67–75Google Scholar
  165. Neuvéglise C, Brygoo Y (1994) Identification of group-I introns in the 28S rDNA of the entomopathogenic fungus Beauveria brongniartii. Curr Genet 27:38–45PubMedGoogle Scholar
  166. Nolan RA (1993) An inexpensive medium for mass fermentation production of Entomophaga aulicae hyphal bodies competent to form conidia. Can J Microbiol 39:588–593Google Scholar
  167. Ohbayashi T, Iwabuchi K (1991) Abnormal behavior of the common armyworm Pseudaletta separata Walker (Lepidopters: Nocutuidae) larvae infected with an en-tomogenous fungus Entomophaga aulicae and a nuclear polyhedrosis virus. Appl Entomol Zool 26:579–586Google Scholar
  168. Panaccione DG, Scott-Craig JS, Pocard J-A, Walton JD (1992) Acyclic peptide synthetase gene required for pathogenicity of the fungus Cochiliobolus carbonum on maize. Proc Natl Acad Sci USA 89:6590–6594PubMedGoogle Scholar
  169. Paris S, Ferron P (1979) Study of the virulence of some mutants of Beauveria brongniartii (= Beauveria tenella). J Invertebr Pathol 34:71–77Google Scholar
  170. Paris S, Ferron P, Fargues J, Robert P (1985) Physiological characteristics and virulence of auxotrophic and morphological mutants of Beauveria brongniartii (Sacc.) Petch (Beauveria tenella). Mycopathologia 91:109–116Google Scholar
  171. Pell JK, Wilding N (1992) The survival of Zoophthora radicans (Zygomycetes: Entomophthorales) isolates as hyphal bodies in mummified larvae of Plutella xylostella (Lep. Yponomeutidae). Entomophaga 37:649–654Google Scholar
  172. Pendland JC, Boucias DG (1986) Lectin binding characteristics of several entomogenous hyphomycetes : Possible relationship to insect hemagglutinins. Mycologia 78: 818–824Google Scholar
  173. Pendland JC, Boucias DG (1992) Ultrastructural localization of carbohydrate in cell walls of the entomogenous hyphomycete Nomuraea rileyi. Can J Microbiol 38: 377–386Google Scholar
  174. Pendland JC, Heath MA, Boucias DG (1988) Function of a galactose-binding lectin from Spodoptera exigua larval haemolymph: opsonization of blastospores from entomogenous hyphomycetes. J Insect Physiol 34:533–540Google Scholar
  175. Pendland JC, Hung S-Y, Boucias DG (1993) Evasion of host defense by in vivo produced protoplast-like cells of the insect mycopathogen Beauveria bassiana. J Bacteriol 175:5962–5969PubMedGoogle Scholar
  176. Pereira RM, Stimac JL (1992) Transmission of Beauveria bassiana with nests of Solenopsis invicta (Hymenoptera : Formicidae) in the laboratory. Environ Entomol 21:1427–1432Google Scholar
  177. Pfeifer TA, Khachatourians GG (1989) Isolation and characterization of DNA from the entomopathogen Beauveria bassiana. Exp Mycol 13:392–402Google Scholar
  178. Pfeifer TA, Khachatourians GG (1992) Beauveria bassiana protoplast regeneration and transformation using electroporation. Appl Microbiol Biotechnol 38:376–381Google Scholar
  179. Pfeifer TA, Khachatourians GG (1993a) Electrophoretic karyotype of the entomopathogenic deuteromycete, Beauveria bassiana. J Invertebr Pathol 62:231–235Google Scholar
  180. Pfeifer TA, Khachatourians GG (1993b) Isolation of DNA from entomopathogenic fungi grown in liquid cultures. J Invertebr Pathol 61:113–116Google Scholar
  181. Pfeifer TA, Khachatourians GG (1993c) Electrophoretic karyotype of the entomopathogenic deuteromycete, Beauveria bassiana. J Invertebr Pathol 61:231–235Google Scholar
  182. Pfeifer TA, Hegedus DD, Khachatourians GG (1993) The mitochondrial genome of the entomopathogenic fungus Beauveria bassiana: analysis of the ribosomal RNA region. Can J Microbiol 39:25–31PubMedGoogle Scholar
  183. Pfeifer TA, Hegedus DD, Khachatourians GG (1995) Assessment of morphological, enzymatic and molecular characteristics for the differentiation of the genera Beauveria and Tolypocladium (Submitted)Google Scholar
  184. Punja ZK, Huang J-S, Jenkins SF (1985) Relationship of mycelial growth and production of oxalic acid and cell wall degrading enzymes to virulence in Sclerotium rolfsii. Can J Plant Pathol 7:109–117Google Scholar
  185. Quiot JM, Vey A, Vago C (1985) Effects of mycotoxins on invertebrate cells in vitro. Adv Cell Cult 4:199–212Google Scholar
  186. Rakotonirainy M, Dutertre M, Brygoo Y, Riba G (1991) rRNA sequence comparison of Beauveria bassiana, Tolypocladium cylindrospora and Tolypocladium ex-tinguens. J Invertebr Pathol 57:17–22PubMedGoogle Scholar
  187. Rakotonirainy M, Cariou ML, Brygoo Y, Riba G (1994) Phylogenetic relationships within the genus Metarhizium based on 28S rRNA sequences and isozyme comparison. Mycol Res 98:225–230Google Scholar
  188. Rath AC, Carr CJ, Graham BR (1995a) Characterization of Metarhizium anisopliae strains by carbohydrate utilization (API50CH). J Invertebr Pathol 65:152–161Google Scholar
  189. Rath AC, Anderson CG, Worledge D, Koen TB (1995b) The effect of low temperatures on the virulence of Metarhizium anisopliae (DAT F 001) to subterranean scarab Adoryphorus couloni. J Invertebr Pathol 65: 186–192Google Scholar
  190. Riba G, DeAzevedo JL, Messias C, Dias DaSilveira W, Tuveson R (1985) Studies of the inheritance of virulence in the entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 46:20–25Google Scholar
  191. Richards EH, Ratcliff NA (1990) Direct binding and lectin mediated binding of erythrocytes to haemocytes of the insect Extatosoma tiaratum. Dev Comp Immunol 14: 269–281PubMedGoogle Scholar
  192. Roberts DW, Hajek AE (1992) Entomopathogenic fungi as bioinsecticides. In: Latham GF (ed) Frontiers in industrial mycology. Chapman & Hall, New York, pp 144–159Google Scholar
  193. Samson RA, Evans HC, Latge J-P (1988) Atlas of ento-mopathogenic fungi. Springer Berlin Heidelberg New YorkGoogle Scholar
  194. Samuels RI, Charnley AK, Reynolds SE (1988a) The role of destruxins in the pathogenicity of 3 strains of Metarhizium anisopliae for the tobacco hornworm Manduca sexta. Mycopathologia 104:54–58Google Scholar
  195. Samuels RI, Charnley AK, Reynolds SE (1988b) Application of reversed-phase HPLC in separation and detection of the cyclodepsipeptide toxins produced by the entomopathogenic fungus Metarhizium anisopliae. J Chromatog Sci 26:15–19Google Scholar
  196. Samuels RI, Reynolds SE, Charnley AK (1988c) Calcium channel activation of insect muscle by destruxins, insecticidal compounds produced by the entopathogenic fungus Metarhizium anisopliae. Comp Biochem Physiol 90C:403–412Google Scholar
  197. Samuels KDZ, Heale JB, Llewellyn M (1988) Characteristics relating to the pathogenicity of Metarhizium anisopliae towards Nilaparvata lugens. J Invert Pathol 53:25–31Google Scholar
  198. Sancehez-Pena SR, Buschinger A, Humber RS (1993) Myrmicinosporidium durum, an enigmatic fungal parasite of ants. J Invertebr Pathol 61:90–96Google Scholar
  199. Schmitz V, Dedryver C-A, Pierre J-S (1993) Influence of an Erynia neoaphidis infection on the relative rate of increase of the cereal aphid Sitobion avenae. J Invertebr Pathol 61:62–68Google Scholar
  200. Scholtens-Toma IMJ, Joosten MHA, DeWit PJGM (1991) Appearance of pathogen-related proteins in plant hosts — relationships between compatible and incompatible interactions. In: Latge JP, Boucias DG (eds) Fungal cell wall and immune response. Springer, Berlin Heidelberg New York, pp 247–265Google Scholar
  201. Shimizu S, Aizawa K (1992) Antigenic structures of blastospores of three entomopathogenic Paecilomyces species and Nomurea rileyi. J Invertebr Pathol 59: 106–108PubMedGoogle Scholar
  202. Shimizu S, Nishida Y, Yoshioka H, Matsumoto T (1991) Separation of chromosomal DNA molecules from Paecilomyces fumosoroseus by pulse field electrophoresis. J Invertebr Pathol 58:461–463Google Scholar
  203. Shimizu S, Higashiyama R, Matsumoto T (1993a) Chromosome length polymorphism in Beauveria bassiana. J Seric Sci Jpn 62:45–49Google Scholar
  204. Shimizu S, Tsuchitani Y, Matsumoto T (1993b) Production of an extracellular protease by Beauveria bassiana in the haemolymph of the silkworm, Bombyx mori. Lett Appl Microbiol 16:291–294Google Scholar
  205. Shimizu S, Tsuchitani Y, Matsumoto T (1993c) Serology and substrate specificity of extracellular proteases from four species of entomopathogenic hyphomycetes. J Invertebr Pathol 61:192–195Google Scholar
  206. Shimizu S, Yoshioka H, Matsumoto T (1993d) Electro-phoretic karyotyping of the entomogenous fungus Paecilomyces fumosoroseus. Lett Appl Microbiol 16: 183–186Google Scholar
  207. Siebeneicher SR, Vinson SB, Kenerley CM (1992) Infection of the red imported fire ants by Beauveria bassiana through various routes of exposure. J Invertbr Pathol 59:280–285Google Scholar
  208. Sloman IS, Reynolds SE (1993) Inhibition of ecdysteroid secretion from Manduca prothoracic glands in vitro by destruxins — cyclic depsipeptide toxins from the insect pathogenic fungus Metarhizium anisopliae. Insect Biochem Mol Biol 23:43–46Google Scholar
  209. Steinkraus DC, Geden CJ, Rutz DA (1991) Susceptibility of lesser mealworm (Coleoptera : Tenebrionid) to Beauveria bassiana (Moniliales:Moniliaceae): effects on host stage, substrate, formulation, and host passage. J Med Entomol 28:314–321Google Scholar
  210. St Leger RJ, Durrands PK, Cooper RM, Charnley AK (1988) Regulation of production of proteolytic enzymes by the entomopathogenic fungus Metarhizium anisopliae. Arch Microbiol 150:413–416Google Scholar
  211. St Leger RJ, Staples RC, Roberts DW (1993) Entomopathogenic isolates of Metarhizium anisopliae, Beauveria bassiana and Aspergillus flavus produce multiple extracellular chitinase isozymes. J Invertebr Pathol 61:81–84Google Scholar
  212. Storck R, Alexopoulos CJ (1970) Deoxyribonucleic acid of fungi. Bacteriol Rev 34:126–154PubMedGoogle Scholar
  213. Strongman DB, MacKay RM (1993) Discrimination between Hirsutella longicolla var. longicolla and Hirsutella longicolla var. cornuta using random amplified polymorphic DNA fingerprinting. Mycologia 85:65–70Google Scholar
  214. Strongman DB, Strunz GM, Giguere P, Yu C-M, Calhoun L (1987) Enniatins from Fusarium avenaceum isolated from balsam fir foliage and their toxicity to spruce budworm larvae, Choristoneura fumiferana Clem. J Chem Ecol 14:753–764Google Scholar
  215. Talbot NJ, Salch YP, Ma M, Hamer JE (1993) Karyotypic variation within clonal linages of the rice blast fungus Magnaporthegrisea. Appl Environ Microbiol 59:585–593PubMedGoogle Scholar
  216. Tan Y-C, Ekramoddoullah KM (1991) Immunochemical characterization of the entomopathogenic fungus Beauveria bassiana. J Invertbr Pathol 57:269–276Google Scholar
  217. Thomas KC, Khachatourians GG, Ingledew WM (1987) Production and properties of Beauveria bassiana conidia cultivated in submerged culture. Can J Microbiol 33: 12–20Google Scholar
  218. Tigano-Milani MS, Gomes ACMM, Sobral BWS (1995) Genetic variability among Brazilian isolates of the entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 65:206–210Google Scholar
  219. Typas MA, Griffin AM, Bainbridge BW, Heale JB (1992) Restriction fragment length polymorphism in mitochondrial DNA and ribosomal RNA gene complexes as an aid to the characterization of species and sub-species populations in the genus Verticillium. FEMS Microbiol Lett 95:157–162Google Scholar
  220. Urbanczyk MJ, Zabza A, Balazy S, Peczynska-Czoch W (1992) Laboratory culture media and enzyme activity of some entomopathogenic fungi of Zoophthora (Zygomcetes:Enthomopthoraceae). J Invertebr Pathol 59: 250–257Google Scholar
  221. Vandenberg JD (1990) Safety of four entomopathogens for caged adult honeybees (Hymenoptera : Aphidae) J Econ Entomol 83:755–759Google Scholar
  222. Van den Boogert PHJF, Dijksterhuis J, Velvis H, Veenhuis M (1992) Adhesive knob formation by conidida of the nematophagous fungus Drechmeria coniospora. Antonie Leeuwenhoek Int J Gen Mol Microbiol 61: 221–229Google Scholar
  223. Vey AJ, Fargues J (1977) Histological and ultrastructural studies of Beauveria bassiana infection in Leptinotarsa decemlineta larvae during ecdysis. J Invertebr Pathol 30:207–215Google Scholar
  224. Vey AJ, Quiot J-M, Pais M (1986) Toxémie d’origine fongique chez les invertèbres et ses consequences cytotoxiques: étude sur l’infection a Metarhizium anisopliae (Hyphomycete, Moniliales) chez les lepidoptères et les coleoptères. CR Soc Biol 180:105–112Google Scholar
  225. Vey A, Quiot J-M, Vago C (1987) Mode d’action insecticide d’une mycotoxine, la Destruxine E, sur les diptères vecteurs et disseminateurs de germes. CR Acad Sci (Paris) Ser 3 304:229–234Google Scholar
  226. Vey A, Quiot JM, Mazet I, McCoy CW (1993) Toxicity and pathology of crude broth filterate produced by Hirsutella thompsoniivar thompsonii in shake culture. J Invertebr Pathol 61:131–137PubMedGoogle Scholar
  227. Villani MG (1992) Behavioral response of scarabs to inun-dative release of entomopathogens in the turfgrass microcosms: implication for consistent results in the field. 25th Annu Meet Soc Invertbr Pathol, Heidelberg, Germany, Aug 16–21, 178 ppGoogle Scholar
  228. Vincent MJ, Miranpuri GS, Khachatourians GG (1993) Acid phosphatase activity of the migratory grasshopper, Melanoplus sanguinipes during Beauveria bassiana infection. Entomol Exp Appl 67:161–166Google Scholar
  229. von Arx JA (1986) Tolypocladium, a synonym of Beauveria. Mycotaxon 25:153–158Google Scholar
  230. Wahlman M, Davidson BS (1993) New destruxins from the entomopathogenic fungus Metarhizium anisopliae. J Nat Prod 56:643–647Google Scholar
  231. Walsh SRA, Tyrell D, Humber RA, Silver JC (1990) DNA restriction fragment length polymorphisms in the rDNA repeat unit of Entomophaga. Exp Mycol 14: 381–392Google Scholar
  232. Warburg A (1990) Entomopathogens of phlebotomine sand flies: laboratory experiments and natural infections. J Invertbr Pathol 58:189–202Google Scholar
  233. Watson DW, Mellens BA, Petersen JJ (1993) Behavioral response of Musca domestica (Diptera : Muscidae) to infection by Entomophthora muscae (Zygomymetes : Entomophthorales). J Invertebr Pathol 61:10–16Google Scholar
  234. Weiser J, Matha V (1988) Tolypin, a new insecticidal metabolite of fungi of the genus Tolypocladium. J Invertebr Pathol 51:94–96PubMedGoogle Scholar
  235. Wheeler MB, Stuart GS, Hapner KD (1993) Agglutinin mediated opsonization of fungal blastospores in Melanoplus differentialis (Insecta). J Insect Physiol 39: 477–483Google Scholar
  236. Wright JE, Chandler LD (1991) Laboratory evaluation of the entomopathogenic fungus Beauveria bassiana against the boll weevil (Cutculinidae:Coleoptera). J Invertbr Pathol 58:448–449Google Scholar
  237. Yoshida S, Yamashita M, Yonihara S, Eguchi M (1990) Properties of fungal protease inhibitors from the integument and hemolymph of the silkworm and effect of an inhibitor of fungal growth. Comp Biochem Physiol 95:559–564Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. G. Khachatourians
    • 1
  1. 1.Bioinsecticide Research Laboratory, Department of Applied Microbiology and Food ScienceUniversity of SaskatchewanSaskatoonCanada

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