Regulation of Trehalose Metabolism and Its Relevance to cell Growth and Function

  • J. M. Thevelein
Part of the The Mycota book series (MYCOTA, volume 3)

Abstract

Trehalose is a disaccharide (α-d-glucopyranosyl α-d-glucopyranoside) commonly found in fungi and present at particularly high concentrations in resting cells and survival forms, such as spores and sclerotia. Two specific lines of research with respect to trehalose have received much attention. The first is in control of trehalose mobilization during the initiation of growth in resting cells and, more recently, the possible role of trehalose as a stress protectant. With respect to trehalose mobilization in fungi, two mechanisms have been proposed to trigger its onset, depending on the type of trehalase present in a particular species.

Keywords

Sugar Fermentation Carbohydrate Sponge Fructose 

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References

  1. Alabran DM, Ball DH, Reese ET (1983) Comparison of the trehalase of Trichoderma reesei with those from other sources. Carbohydr Res 123: 179–181PubMedCrossRefGoogle Scholar
  2. Anchordoguy TJ, Crowe JH, Griffin FJ, Clark WH (1988) Cryopreservation of sperm from the marine shrimp Sicyona engentis. Cryobiology 25: 238–243PubMedCrossRefGoogle Scholar
  3. App H, Holzer H (1989) Purification and characterization of neutral trehalase from the yeast ABYS1 mutant. J Biol Chem 264: 17583–17588PubMedGoogle Scholar
  4. Arguelles JC, Gacto M (1985) Evidence for regulatory trehalase activity in Candida utilis. Can J Microbiol 31: 529–537CrossRefGoogle Scholar
  5. Arguelles JC, Gaeta M (1986) Comparative study of two trehalases from Candida utilis. Microbiologia 2: 105–114PubMedGoogle Scholar
  6. Arguelles JC, Gacto M (1988) Differential location of regulatory and non-regulatory trehalases in Candida utilis cells. Antonie Leeuwenhoek 54: 555–565PubMedCrossRefGoogle Scholar
  7. Arguelles JC, Vicente-Soler J, Gacto M (1986) Protein phosphorylation and trehalase activation in Candida utilis. FEMS Microbiol Lett 34: 361–365CrossRefGoogle Scholar
  8. Attfield PV (1987) Trehalose accumulates in Saccharomyces cerevisiae during exposure to agents that induce heat shock response. FEBS Lett 225: 259–263PubMedCrossRefGoogle Scholar
  9. Attfield PV, Raman A, Northcott CJ (1992) Construction of Saccharomyces cerevisiae strains that accumulate relatively low concentrations of trehalose, and their application in testing the contribution of the disaccharide to stress tolerance. FEMS Microbiol Lett 94: 271–276CrossRefGoogle Scholar
  10. Barton JK, den Hollander JA, Hopfield JJ, Shulman RG (1982) 13C nuclear magnetic resonance study of trehalose mobilization in yeast spores. J Bacteriol 151: 177–185Google Scholar
  11. Becher dos Passos J, Vanhalewyn M, Brandao RL, Castro IM, Nicoli JR, Thevelein JM (1992) Glucose-induced activation of plasma membrane HF-ATPase in mutants of the yeast Saccharomyces cerevisiae affected in cAMP metabolism, cAMP-dependent protein phosphorylation and the initiation of glycolysis. Biochim Biophys Acta 1136: 57–67CrossRefGoogle Scholar
  12. Becker JU, Shehata MI, Mizani SM (1982) Influence of nitrogen sources on glycogen metabolism in Saccharomyces carlsbergensis. J Gen Microbiol 128: 455–461Google Scholar
  13. Belazzi T, Wagner A, Wieser R, Schanz M, Adam G, Hartig A, Ruis H (1991) Negative regulation of transcription of the Saccharomyces cerevisiae catalase T (CTTI) gene by cAMP is mediated by a positive control element. EMBO J 10: 585–592PubMedGoogle Scholar
  14. Bell W, Klaassen P, Ohnacker M, Boller T, Herweijer M, Schoppink P, van der Zee P, Wiemken A (1992) Characterization of the 56kDa subunit of trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF], a regulator of carbon catabolite inactivation. Eur J Biochem 209: 951–959PubMedCrossRefGoogle Scholar
  15. Bhandal IS, Hauptmann RM, Widholm JM (1985) Trehalose as cryoprotectant for the freeze preservation of carrot and tobacco cells. Plant Physiol 78: 430–432PubMedCrossRefGoogle Scholar
  16. Bissinger PH, Wieser R, Hamilton B, Ruis H (1989) Control of Saccharomyces cerevisiae catalase T gene (CTT1) expression by nutrient supply via the RAS-cyclic AMP pathway. Mol Cell Biol 9: 1309–1315PubMedGoogle Scholar
  17. Blakeley D, Tolliday B, Colaço C, Roser B (1990) Dry instant blood typing plate for bedside use. Lancet 336: 854–855PubMedCrossRefGoogle Scholar
  18. Blazquez MA, Stucka R, Feldmann H, Gancedo C (1992) Isolation in Schizosaccharomyces pombe of a homolog of the Saccharomyces cerevisiae CIF] gene. In: Worksh on control of gene expression in yeast. The Centre for International Meetings on Biology, Instituto Juan March de Estudios e Investigaciones 9: 60Google Scholar
  19. Boiteux A (1992) Metabolic studies on synchronously dividing yeast cells. Energy metabolism during cellular division. Proc 10th Small Meet on Yeast — Transport and Energetics. Marburg, Germany, p 28Google Scholar
  20. Boorstein WR, Craig EA (1990) Regulation of a yeast HSP70 gene by a cAMP responsive transcriptional control element. EMBO J 9: 2543–2553PubMedGoogle Scholar
  21. Boos W, Ehmann U, Bremer E, Middendorf A, Postma P (1987) Trehalase of Escherichia coll. Mapping and cloning of its structural gene and identification of the enzyme as a periplasmic protein induced under high osmolarity growth conditions. J Biol Chem 262: 13212–13218Google Scholar
  22. Bourret JA (1986) Evidence that a glucose-mediated rise in cyclic AMP triggers germination of Piloholus longipes spores. Exp Mycol 10: 60–66CrossRefGoogle Scholar
  23. Brana AF, Mendez C, Diaz LA, Manzanal MB, Hardisson C (1986) Glycogen and trehalose accumulation during colony development in Streptomyces antibioticus. J Gen Microbiol 132: 1319–1326PubMedGoogle Scholar
  24. Breedveld MW, Zevenhuizen LPTM, Zehnder AJB (1991) Osmotically regulated trehalose accumulation and cyclic beta-(1,2)-glucan excretion by Rhizobium leguminosarum biovar trifolii TA-1. Arch Microbiol 156: 501506Google Scholar
  25. Broach JR, Deschenes RJ (1990) The function of RAS genes in Saccharomyces cerevisiae. Adv Cancer Res 54: 79–139PubMedCrossRefGoogle Scholar
  26. Brownlee C, Jennings DH (1981) The content of soluble carbohydrates and their translocation in mycelium of Serpula lacrimans. Trans Br Mycol Soc 77: 615–619CrossRefGoogle Scholar
  27. Burke MJ (1985) The glassy state and survival of anhydrous biological systems. In: Leopold AC (ed) Membranes, metabolism and dry organisms. Cornell Univ Press, Ithaca, NY, pp 358–363Google Scholar
  28. Cabib E, Leloir LF (1958) The biosynthesis of trehalose phosphate. J Biol Chem 231: 259–275PubMedGoogle Scholar
  29. Callaerts G, Iserentant D, Verachtert H (1993) Relation between trehalose and sterol accumulation during oxygenation of cropped yeast. J Am Soc Brew Chem 51: 7577Google Scholar
  30. Cameron S, Levin L, Zoller M, Wigler M (1988) cAMPindependent control of sporulation, glycogen metabolism, and heat shock resistance in S. cerevisiae. Cell 53: 555–566Google Scholar
  31. Carrillo D, Vicente-Soler J, Gacto M (1992) Activation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schizosaccharomyces pombe. FEMS Microbial Lett 98: 61–66CrossRefGoogle Scholar
  32. Charlab R, Oliveira DE, Panek AD (1985) Investigation of the relationship between sstl and fdp mutations in yeast and their effect on trehalose synthesis. Braz J Med Biol Res 18: 447–454PubMedGoogle Scholar
  33. Cherry JR, Johnson TR, Dollard C, Shuster JR, Denis CL (1989) Cyclic AMP-dependent protein kinase phosphorylates and inactivates the yeast transcriptional activator ADR1. Cell 56: 409–419PubMedCrossRefGoogle Scholar
  34. Clegg JS (1985) The physical properties and metalic status of Artemia cysts at low water contents: the “water replacement hypothesis”. In: Leopold AC (ed) Membranes, metabolism and dry organisms. Cornell Univ Press, Ithaca, NY, pp 169–187Google Scholar
  35. Cochrane VW (1958) The physiology of fungi. Wiley, New YorkGoogle Scholar
  36. Colaço C, Sen S, Thangavelu M, Pinder S, Roser B (1992) Extraordinary stability of enzymes dried in trehalose: simplified molecular biology. Biotechnology 10: 10071011Google Scholar
  37. Coote PJ, Jones MV, Edgar K, Cole MB (1992) TPK gene products mediate cAMP-independent thermotolerance in Saccharomyces cerevisiae. J Gen Microbial 138: 25512557Google Scholar
  38. Costa-Carvalho VLA, Panek AD, Rocha-Ledo MHM (1983) Glycogen accumulation by Saccharomyces cerevisiae: influence of specific growth rate. IRCS Med Sci 11: 120–121Google Scholar
  39. Cotter DA (1975) Spores of the cellular slime mold Dictyostelium discoideum. In: Gerhardt P, Costilow RN, Sadoff HL (eds) Spores VI. Am Soc Microbiol, Washington, DC, pp 61–72Google Scholar
  40. Coutinho C, Bernardes E, Felix D, Panek A (1988) Trehalose as cryoprotectant for preservation of yeast strains. J Biotechnol 7: 23–32CrossRefGoogle Scholar
  41. Coutinho CC, Silva JT, Panek AD (1992) Trehalase activity and its regulation during growth of Saccharomyces cerevisiae. Biochem Int 26: 521–530PubMedGoogle Scholar
  42. Crowe JH, Crowe LM, Chapman D (1984) Preservation of membranes in anhydrobiotic organisms. Science 223: 701–703PubMedCrossRefGoogle Scholar
  43. Crowe JH, Carpenter JF, Crowe LM, Anchordoguy TJ (1990) Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiology 27: 219–231CrossRefGoogle Scholar
  44. Crowe JH, Panek AD, Crowe LM, Panek AC, Dearaujo PD (1991) Trehalose transport in yeast cells. Biochem Int 24: 721–730PubMedGoogle Scholar
  45. Crowe JH, Hoekstra FA, Crowe LM (1992) Anhydrobiosis. Annu Rev Physiol 54: 579–599CrossRefGoogle Scholar
  46. De Antoni GL, Perez P, Abraham A, Anon MC (1989) Trehalose, a cryoprotectant for Lactobacillus bulgaricus. Cryobiology 26: 149–153CrossRefGoogle Scholar
  47. De Araujo PS, Panek AC, Crowe JH, Crowe LM, Panek AD (1991) Trehalose-transporting membrane vesicles from yeasts. Biochem Int 24: 731–737PubMedGoogle Scholar
  48. De Koning W, Groenveld K, Oehlen LJWM, Berden JA, Van Dam K (1991) Changes in the activities of key enzymes of glycolysis during the cell cycle in yeast: a rectification. J Gen Microbiol 137: 971–976PubMedCrossRefGoogle Scholar
  49. Dellamora-Ortiz GM, Ortiz CHD, Maia JCC, Panek AD (1986) Partial purification and characterization of the interconvertible forms of trehalase from Saccharomyces cerevisiae. Arch Biochem Biophys 251: 205–214PubMedCrossRefGoogle Scholar
  50. De Virgilio C, Simmen U, Hottiger T, Boller T, Wiemken A (1990) Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schizosaccharomyces pombe, even in the presence of cycloheximide. FEBS Lett 273: 107–110PubMedCrossRefGoogle Scholar
  51. De Virgilio C, Bürckert N, Boller T, Wiemken A (199la) A method to study the rapid phosphorylation-related modulation of neutral trehalase activity by temperature shifts in yeast. FEBS Lett 291: 355–358Google Scholar
  52. De Virgilio C, Muller J, Boller T, Wiemken A (1991b) A constitutive, heat shock-activated neutral trehalase occurs in Schizosaccharomyces pombe in addition to the sporulation-specific acid trehalase. FEMS Microbiol Lett 84: 85–90Google Scholar
  53. De Virgilio C, Piper P, Boller T, Wiemken A (1991e) Acquisition of thermotolerance in Saccharomyces cerevisiae without heat shock protein hsp-104 and in the absence of protein synthesis. FEBS Lett 288: 86–90PubMedCrossRefGoogle Scholar
  54. De Virgilio C, Bürckert N, Bell W, Jenti B, Boller T, Wiemken A (1993) Disruption of TPS2, the gene encoding the 100 kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity. Eur J Biochem 212: 315–323PubMedCrossRefGoogle Scholar
  55. Dewerchin MA, Van Laere AJ (1984) Trehalase activity and cyclic AMP content during early development of Mucor rounii spores. J Bacteriol 158: 575–579PubMedGoogle Scholar
  56. Donnini C, Puglisi PP, Vecli A, Marmiroli N (1988) Germination of Saccharomyces cerevisiae ascospores without trehalose mobilization as revealed by in vivo 13C nuclear magnetic resonance spectroscopy. J Bacteriol 170: 37893791Google Scholar
  57. Dumont JE, Jauniaux JC, Roger PP (1989) The cyclic AMP-mediated stimulation of cell proliferation. Trends Biochem Sci 14: 67–71PubMedCrossRefGoogle Scholar
  58. Elbein AD (1974) The metabolism of a-a-trehalase. Adv Carbohydr Chem Biochem 30: 227–256PubMedCrossRefGoogle Scholar
  59. Elliott B, Futcher B (1993) Stress resistance of yeast cells is largely independent of cell cycle phase. Yeast 9: 33–42PubMedCrossRefGoogle Scholar
  60. Emyanitoff RG, Wright BE (1979) Effect of intracellular carbohydrates on heat resistance of Dictyostelium descoideum spores. J Bacteriol 140: 1008–1012PubMedGoogle Scholar
  61. Engelberg D, Perlman R, Levitzki A (1989) Transmembrane signalling in Saccharomyces cerevisiae. Cell Signalling 1: 1–7PubMedCrossRefGoogle Scholar
  62. Engelberg D, Poradosu E, Simchen E. Levitzki A (1990) Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides. FEBS Lett 261: 413–418PubMedGoogle Scholar
  63. Farkas I, Hardy TA, Depaoliroach AA, Roach PJ (1990) Isolation of the GSYI gene encoding yeast glycogen synthase and evidence for the existence of a second gene. J Biol Chem 265: 20879–20886PubMedGoogle Scholar
  64. Farkas I, Hardy TA, Goebl MG. Roach PJ (1991) Two glycogen synthase isoforms in Saccharomyces cerevisiae are coded by distinct genes that are differentially controlled. J Biol Chem 266:15602–15607Google Scholar
  65. François J, Van Schaftingen E, Hers H-G (1984) The mechanism by which glucose increases frutose-2,6hisphosphate concentration in Saccharomyces cerevisiae. A cyclic-AMP-dependent activation of phosphofructokinase 2. Eur J Biochem 145: 187–193PubMedCrossRefGoogle Scholar
  66. François J, Eraso P, Gancedo C (1987) Changes in the concentration of cAMP, frutose-2,6-bisphosphate and related metabolites and enzymes in Saccharomyces cerevisiae during growth on glucose. Eur J Biochem 164: 369–373PubMedCrossRefGoogle Scholar
  67. François J, Neves M-J, Hers H-G (1991) The control of trehalose biosynthesis in Saccharomyces cerevisiae: evidence for a catabolite inactivation and repression of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase. Yeast 7: 575–587PubMedCrossRefGoogle Scholar
  68. Franks F. Hatley RHM, Mathias SF (1991) Materials science and the production of shelf-stable biologicals. Biopharm 4: 38–42Google Scholar
  69. Fukui Y, Kozasa T, Kaziro Y, Takeda T, Yamamoto M (1986) Role of a ras homolog in the life cycle of Schizosaccharomyces pombe. Cell 44: 329–336PubMedCrossRefGoogle Scholar
  70. Gadd GM, Chalmers K. Reed RH (1987) The role of trehalose in dehydration resistance of Saccharomyces cerevisiae. FEMS Microbial Lett 48: 249–254CrossRefGoogle Scholar
  71. Gélinas P, Fiset G, LeDuy A, Goulet J (1989) Effect of growth conditions and trehalose content on cryotolerance of bakers’ yeast in frozen doughs. Appl Environ Microbiol 55: 2453–2459PubMedGoogle Scholar
  72. Giver HM, Styrvold OB, Kaasen J. Strom AR (1988) Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli. J Bacteriol 170: 2841–2849Google Scholar
  73. Gibbs JB, Marshall MS (1989) The ras oncogene — an important regulatory element in lower eucaryotic organisms. Microbial Rev 53: 171–185Google Scholar
  74. Gonzalez MI. Stucka R, Blazquez MA, Feldmann H, Gancedo C (1992) Molecular cloning of CIFI,a yeast gene necessary for growth on glucose. Yeast 8:183192Google Scholar
  75. Gottlieb D (1978) The germination of fungus spores. Merrow, Newcastle-upon-Tyne, Meadowfie Id Press, ShildonGoogle Scholar
  76. Grba S, Oura E, Suomalainen H (1975) On the formation of glycogen and trehalose in baker’s yeast. Eur J Appl Microbiol 2: 29–37CrossRefGoogle Scholar
  77. Grba S, Oura E, Suomalainen H (1979) Formation of trehalose and glycogen in growing baker’s yeast. Finn Chem Lett 1979: 61–64Google Scholar
  78. Green JL, Angell CA (1989) Phase relations and vitrification in saccharide-water solutions and the trehalose anomaly. J Phys Chem 93: 2880–2882CrossRefGoogle Scholar
  79. Gupta J, Harris SD, Cotter DA (1987) Evidence for nonregulatory trehalase activity in Dictyostelium discoideum. Curr Microbiol 16: 101–104CrossRefGoogle Scholar
  80. Gutierrez C, Ardourel M, Bremer E, Middendorf A, Boos W, Ehmann U (1989) Analysis and DNA sequence of the osmoregulated treA gene encoding the periplasmic trehalase of Escherichia coil K12. Mol Gen Genet 217: 347–354PubMedCrossRefGoogle Scholar
  81. Hall BG (1983) Yeast thermotolerance does not require protein synthesis. J Bacteriol 156: 1363–1365PubMedGoogle Scholar
  82. Hammond JBW, Nichols R (1976) Carbohydrate metabolism in Agaricus bisporus (Lange) Sing.: changes in soluble carbohydrate during growth of mycelium and sporphore. J Gen Microbiol 95: 309–320Google Scholar
  83. Harris DS, Cotter DA (1987) Vacuolar (lysosomal) trehalase of Saccharomyces cerevisiae. Curr Microbiol 15: 247–249CrossRefGoogle Scholar
  84. Hengge-Aronis R, Klein W, Lange R, Rimmele M, Boos W (1991) Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coll. J Bacteriol 173: 7918–7924PubMedGoogle Scholar
  85. Hino A, Mihara K, Nakashima K, Takano H (1990) Trehalose levels and survival ratio of freeze-tolerant versus freeze-sensitive yeasts. Appl Environ Microbiol 56: 1386–1391PubMedGoogle Scholar
  86. Hirimburegama K, Durnez P, Keleman J, Oris E, Vergauwen R, Mergelsberg H, Thevelein JM (1992) Nutrient-induced activation of trehalase in nutrient-starved cells of the yeast Saccharomyces cerevisiae: cAMP is not involved as second messenger. J Gen Microbiol 138: 2035–2043PubMedCrossRefGoogle Scholar
  87. Hohmann S, Huse K, Valentin E, Mbonyi K, Thevelein JM, Zimmermann FK (1992) Glucose-induced regulatory defects in the Saccharomyces cerevisiae growth initiation mutant bypl and identification of MIGI as a partial suppressor. J Bacteriol 174: 4183–4188PubMedGoogle Scholar
  88. Hohmann S, Neves MJ, de Koning W, Alijo R, Ramos J, Thevelein JM (1993) The growth and signalling defects of the ggsl (fdpllbypl) deletion mutant on glucose are suppressed by a deletion of the gene encoding hexokinase PII. Curr Genet 23: 281–289PubMedCrossRefGoogle Scholar
  89. Honadel TE, Killian GJ (1988) Cryopreservation of murine embryos with trehalose and glycerol. Cryobiology 25: 331–337PubMedCrossRefGoogle Scholar
  90. Hottiger T, Boller T, Wiemken A (1987a) Rapid changes of heat and desiccation tolerance correlated with changes of trehalose content in Saccharomyces cerevisiae cells subjected to temperature shifts. FEBS Lett 220: 113–115PubMedCrossRefGoogle Scholar
  91. Hottiger T, Schmutz P, Wiemken A (1987b) Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae. J Bacteriol 169: 5518–5522PubMedGoogle Scholar
  92. Hottiger T, Boller T, Wiemken A (1989) Correlation of trehalose content and heat resistance in yeast mutants altered in the RAS/adenylate cyclase pathway: is trehalose a thermoprotectant? FEBS Lett 255: 431434Google Scholar
  93. Hottiger T, De Virgilio C, Bell W, Boller T, Wiemken A (1992) Canavanine treatment of yeast induces thermotolerance. Yeast 8 (Spec Iss): S91Google Scholar
  94. Iida H (1988) Multistress resistance of Saccharomyces cerevisiae is generated by insertion of retrotransposon Ty into the 5’ coding region of the adenylate cyclase gene. Mol Cell Biol 8: 5555–5560PubMedGoogle Scholar
  95. Iida H, Yahara I (1984) Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the Go of higher eukaryotes. J Cell Biol 98: 1185–1193PubMedCrossRefGoogle Scholar
  96. Inoue H, Shimoda C (1981a) Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe. Arch Microhiol 129: 19–22CrossRefGoogle Scholar
  97. Inoue H, Shimoda C (1981 b) Induction of trehalase activity on a nitrogen-free medium: a sporulation-specific event in the fission yeast, Schizosaccharomyces pombe. Mol Gen Genet 183: 32–36Google Scholar
  98. Jacquet M, Camonis 1 (1985) Contrôle du cycle de divisiion cellulaire et de la sporulation chez Saccharomyces cerevisiae par le système de I’AMP cyclique. Biochimie 67: 35–43Google Scholar
  99. Kaasen I, Falkenberg P, Styrvold OB, Strom AR (1992) Moecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coll. Evidence that transcription is activated by katF (appR). J Bacteriol 174: 889–898Google Scholar
  100. Keller F, Schellenberg M, Wiemken A (1982) Localization of trehalase in vacuoles and trehalose in the cytosol of yeast (Saccharomyces cerevisiae). Arch Microhiol 131: 298–301CrossRefGoogle Scholar
  101. Killick KA, Wright BE (1972a) Trehalose synthesis during differentiation in Dictyostelium discoideum. HI. In vitro unmaking of trehalose 6-phosphate synthetase. J Biol Chem 247: 2967–2969Google Scholar
  102. Killick KA, Wright BE (1972b) Trehalose synthesis during differentiation in Dictyostelium discoideum. IV. Secretion of trehalase and in vitro expression of trehalose 6-phosphate synthetase activity. Biochem Biophys Res Commun 48: 1476–1481PubMedCrossRefGoogle Scholar
  103. Kline L, Sugihara TF (1968) Factors affecting the stability of frozen bread doughs. I. Prepared by the straight dough method. Bakers Dig 42: 44–50Google Scholar
  104. Kobayashi N, McEntee K (1993) Identification of cis and trans components of a novel heat shock stress regulatory pathway in Saccharomyces cerevisiae. Mol Cell Biol 13: 248–256PubMedGoogle Scholar
  105. Kopp M, Müller H, Holzer H (1993) Molecular analysis of the neutral trehalase gene from Saccharomyces cerevisiae. J Biol Chem 268: 4766–4774PubMedGoogle Scholar
  106. Kotyk A, Michaljanicova D (1979) Uptake of trehalose by Saccharomyces cerevisiae. J Gen Microbiol 110: 323–332PubMedCrossRefGoogle Scholar
  107. Küenzi MT, Fiechter A (1969) Changes in carbohydrate composition and trehalase activity during the budding cycle of Saccharomyces cerevisiae. Arch Mikrobiol 64: 396–407PubMedCrossRefGoogle Scholar
  108. Küenzi MT, Fiechter A (1972) Regulation of carbohydrate composition of Saccharomyces cerevisiae under growth limitation. Arch Mikrobiol 84: 254–265PubMedCrossRefGoogle Scholar
  109. Levine H, Slade L (1992) Another view of trehalose for drying and stabilizing biological materials. Biopharm 5: 36–40Google Scholar
  110. Lewis JG, Learmonth RP, Watson K (1993) Role of growth phase and ethanol in freeze-thaw stress resistance of Saccharomyces cerevisiae. Appl Environ Microhiol 59: 1065–1071Google Scholar
  111. Lillie SH, Pringle JR (1980) Reserve carbohydrate metabolism in Saccharomyces cerevisiae. Response to nutrient limitation. J Bacteriol 143: 1384–1394Google Scholar
  112. Londesborough J, Varimo K (1984) Characterization of two trehalases in baker’s yeast. Biochem J 219:511–518 Londesborough J, Vuorio 0 (1991) Trehalose-6-phosphate synthase/phosphatase complex from backers’ yeast: purification of a proteolytically activated form. J Gen Microbiol 137: 323–330Google Scholar
  113. Ma H, Botstein D (1986) Effects of null mutations in the hexokinase genes of Saccharomyces cerevisiae on catabolite repression. Mol Cell Biol 6: 4046–4052PubMedGoogle Scholar
  114. Mackay MA, Norton RS, Borowitzka LJ (1984) Organic osmoregulatory solutes in cyanobacteria. J Gen Microbiol 130: 2177–2191Google Scholar
  115. Mackenzie KF, Singh KK, Brown AD (1988) Water stress plating hypersensitivity of yeasts: protective role of trehalose in Saccharomyces cerevisiae. J Gen Microbiol 134: 1661–1666PubMedGoogle Scholar
  116. Mager WH, Moradas Ferreira P (1993) Stress response of yeast. Biochem J 290: 1–13PubMedGoogle Scholar
  117. Malone RE (1990) Dual regulation of meiosis in yeast. Cell 61: 375–378PubMedCrossRefGoogle Scholar
  118. Marchler G, Schuller C, Wieser R, Adam G, Ruis H (1992) A heat shock factor-independent stress control element of the Saccharomyces cerevisiae CTTI promotor regulated by protein kinase A, nitrogen starvation and heat shock. Yeast 8 (Spec Iss): 5154Google Scholar
  119. Marino C, Curto M, Bruno R, Rinaudo MT (1989) Trehalose synthase and trehalose behaviour in yeast cells in anhydrobiosis and hydrobiosis. Int J Biochem 21: 1369–1375CrossRefGoogle Scholar
  120. Martegani E, Baroni M, Vanoni M (1986) Interaction of cAMP with the CDC25-mediated step in the cell cycle of budding yeast. Exp Cell Res 162: 544–548PubMedCrossRefGoogle Scholar
  121. Martin MC, Diaz LA, Manzanal MB, Hardisson C (1986) Role of trehalose in the spores of Streptomyces. FEMS Microbiol Lett 35: 49–54CrossRefGoogle Scholar
  122. Matsumoto K, Uno I, Ishikawa T (1985) Genetic analysis of the role of cAMP in yeast. Yeast 1: 15–24PubMedCrossRefGoogle Scholar
  123. Mbonyi K, Van Aelst L, Argüelles JC, Jans AWH, Thevelein JM (1990) Glucose-induced hyperaccumulation of cAMP and absence of glucose repression in yeast strains with reduced activity of cAMP-dependent protein kinase. Mol Cell Biol 10: 4518–4523PubMedGoogle Scholar
  124. McBride MJ, Ensign JC (1987a) Effects of intracellular trehalose content on Streptomyces griseus spores. J Bacteriol 169: 4995–5001PubMedGoogle Scholar
  125. McBridge MJ, Ensign JC (1987b) Metabolism of endogenous trehalose by Streptomyces griseus spores and by spores or cells of other Actinomycetes. J Bacteriol 169: 5002–5007Google Scholar
  126. McDougall J, Kaasen I, Strom AR (1993) A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coil otsA mutant. FEMS Microbiol Lett 107: 25–30PubMedCrossRefGoogle Scholar
  127. Merritt PP (1960) The effect of preparation on the stability and performance of frozen, unbaked, yeast-leavened doughs. Bakers Dig 34: 57Google Scholar
  128. Mittenbühler K, Holzer H (1988) Purification and characterization of acid trehalase from the yeast suc2 mutant. J Biol Chem 263: 8537–8543PubMedGoogle Scholar
  129. Neves MJ, François J (1992) On the mechanism by which a heat shock induces trehalose accumulation in Saccharomyces cerevisiae. Biochem J 288: 859–864PubMedGoogle Scholar
  130. Neves MJ, Jorge JA, François JM, Terenzi HF (1991) Effects of heat shock on the level of trehalose and glycogen. and on the induction of thermotolerance in Neurospora crassa. FEBS Lett 283: 19–22PubMedCrossRefGoogle Scholar
  131. Nikawa J, Cameron S, Toda T, Ferguson KW, Wigler M (1987) Rigorous feedback control of cAMP levels in Saccharomyces cerevisiae. Genes Dev 1: 931–937PubMedCrossRefGoogle Scholar
  132. Oda Y, Uno K, Ohta S (1986) Selection of yeasts for breadmaking by the frozen dough method. Appt Environ Microbiol 52: 941–943Google Scholar
  133. Operti MS, Oliveira DE, Freitas-Valle AB, Oestreicher EG, Mattoon JR, Panek AD (1982) Relationships between trehalose metabolism and maltose utilization in Saccharomyces cerevisiae. Ill. Evidence for alternative pathways of trehalose synthesis. Curr Genet 5:69–76 Ortiz CH, Maia JCC, Tenan MN, Braz-Padrao GR, Mattoon JR, Panek AD (1983) Regulation of yeast trehalase by a monocyclic, cyclic AMP-dependent phosphorylation-dephosphorylation cascade system. J Bacteriol 153: 644–651Google Scholar
  134. Otting G, Liepinsh E, Wuthrick K (1991) Protein hydration in aqueous solution. Science 254: 974–980PubMedCrossRefGoogle Scholar
  135. Padrao GRB, Malamud DR, Panek AD, Mattoon JR (1982) Regulation of energy metabolism in yeast. Inheritance of a pleiotropic mutation causing defects in metabolism of energy reserves, ethanol utilization and formation of cytochrome a.a3. Mol Gen Genet 185: 255261Google Scholar
  136. Panek AD (1963) Function of trehalose in baker’s yeast (Saccharomyces cerevisiae). Arch Biochem Biophys 100: 422–425CrossRefGoogle Scholar
  137. Panek AD, Bernardes EJ (1983) Trehalose: its role in germination of Saccharomyces cerevisiae. Curr Genet 7: 393–397CrossRefGoogle Scholar
  138. Panek AC, de Araujo PS, Neto VM, Panek AD (1987) Regulation of the trehalose-6-phosphate synthase complex in Saccharomyces. Curr Genet 11: 459–465PubMedCrossRefGoogle Scholar
  139. Panek AD, Sampaio AL, Braz GC, Baker SJ, Mattoon JR (1980) Genetic and metabolic control of trehalose and glycogen synthesis. New relationships between energy reserves, catabolite repression and maltose utilization. Cell Mol Biol 25: 345–354Google Scholar
  140. Panek AD, Ferreira R. Panek AC (1989) Comparative studies between the glucose-induced phosphorylation signal and the heat shock response in mutants of Saccharomyces cerevisiae. Biochimie 71: 313–318Google Scholar
  141. Panek AC, Araujo PS, Poppe SC, Panek AD (1990) On the determination of trehalose-6-phosphate synthase in Saccharomyces. Biochem Int 21.695–704Google Scholar
  142. Pardo LA, Sanchez SM, Lazo PS, Ramos S (1991) In vitro activation of the Saccharomyces cerevisiae Ras/ adenylate cyclase system by glucose and some of its analogues. FEBS Lett 290: 43–48PubMedCrossRefGoogle Scholar
  143. Parry JM, Davies PJ, Evians WE (1976) The effects of “cell age” upon the lethal effects of physical and chemical mutagens in the yeast Saccharomyces cerevisiae. Mol Gen Genet 146: 27–35PubMedCrossRefGoogle Scholar
  144. Paschoalin VMF, Costa-Carvalho VLA, Panek AD (1986) Further evidence for the alternative pathway of trehalose synthesis linked to maltose utilization in.Saccharomyces. Curr Genet 10: 725–731PubMedCrossRefGoogle Scholar
  145. Paschoalin VMF, Silva JT, Panek AD (1989) Identification of an ADPG-dependent trehalose synthase in Saccharomyces. Curr Genet 16: 81–87PubMedCrossRefGoogle Scholar
  146. Payen R (1949) Variation des teneurs en glycogène et en tréhalose pendant le séchage de la levure. Can J Res 27B: 749–756CrossRefGoogle Scholar
  147. Pillar TM, Bradshaw RE (1991) Heat shock and stationary phase induce transcription of the Saccharomyces cerevisiae iso-2-cytochrome c gene. Curr Genet 20: 185188Google Scholar
  148. Piper PW, Lockheart A (1988) A temperature-sensitive mutant of Saccharomyces cerevisiae defective in the specific phosphatase of trehalose biosynthesis. FEMS Microbiol Lett 49: 245–250CrossRefGoogle Scholar
  149. Plesset J, Ludwig JR, Cox BS, McLaughlin CS (1987) Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae. J Bacteriol 169: 779–784PubMedGoogle Scholar
  150. Pollock GE, Holmstrom CD (1951) The trehalose content and the quality of active dry yeast. Cereal Chem 28: 498505Google Scholar
  151. Praekelt UM, Meacock PA (1990) HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function. Mol Gen Genet 223: 97–106PubMedCrossRefGoogle Scholar
  152. Reed RH, Borowitzka LJ, Mackay MA, Chudek JA, Forster R, Warr RSC, Moore DJ, Stewart WDP (1986) Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiol Rev 39: 51–56CrossRefGoogle Scholar
  153. Reed SI (1992) The role of p34 kinases in the G1 to S-phase transition. Annu Rev Cell Biol 8: 529–561PubMedCrossRefGoogle Scholar
  154. Rose M, Albig W, Entian KD (1991) Glucose repression in Saccharomyces cerevisiae is directly associated with hexose phosphorylation by hexokinase-PI and hexokinase-PII. Eur J Biochem 199: 511–518PubMedCrossRefGoogle Scholar
  155. Roser BJ (1991a) Trehalose drying: a novel replacement for freeze-drying. Biopharm 5: 44–53Google Scholar
  156. Roser BJ (1991b) Trehalose, a new approach to premium dried foods. Trends Food Sci Technol 2: 166–169CrossRefGoogle Scholar
  157. Roth R (1970) Carbohydrate accumulation during the sporulation of yeast. J Bacteriol 101: 53–57PubMedGoogle Scholar
  158. Roth R, Sussman M (1968) Trehalose 6-phosphate synthetase (uridine diphosphate glucose: D-glucose 6-phosphate 1-glucosyltransferase) and its regulation during slime mold development. J Biol Chem 243: 5081–5087PubMedGoogle Scholar
  159. Rothman-Denes LB, Cabib E (1970) Two forms of yeast glycogen synthetase and their role in glycogen accumulation. Proc Natl Acad Sci USA 66: 967–974PubMedCrossRefGoogle Scholar
  160. Ruf J, Wacker H, James P, Maffia M, Seiler P, Galand G, von Kieckebusch A, Semenza G, Mantei N (1990) Rabbit small intestinal trehalase. Purification, cDNA cloning, expression, and verification of glycosylphosphatidylinositol anchoring. J Biol Chem 265: 15034–15039Google Scholar
  161. Sacktor B (1968) Trehalase and the transport of glucose in the mammalian kidney and intestine. Proc Natl Acad Sci USA 60: 1007–1014PubMedCrossRefGoogle Scholar
  162. Sacktor B, Berger SJ (1969) Formation of trehalose from glucose in the renal cortex. Biochem Biophys Res Commun 35: 796–800PubMedCrossRefGoogle Scholar
  163. Saenger W (1989) Structure and dynamics of water surrounding biomolecules. Annu Rev Biophys Chem 16: 93114Google Scholar
  164. Sanchez Y, Lindquist SL (1990) HSP104 required for induced thermotolerance. Science 248: 1112–1115PubMedCrossRefGoogle Scholar
  165. Sanchez Y, Taulien J, Borkovich KA, Lindquist S (1992) Hsp104 is required for tolerance to many forms of stress. EMBO J 11: 2357–2364PubMedGoogle Scholar
  166. Schenberg-Frascino A, Moustacchi E (1972) Lethal and mutagenic effects of elevated temperature on haploid yeast. Mol Gen Genet 115: 243–257PubMedCrossRefGoogle Scholar
  167. Semenza G (1981) Intestinal oligo-and disaccharidases. In: Randle PJ, Steiner DF, Whelan WJ (eds) Carbohydrate metabolism and its disorders, vol 3. Academic Press, London, pp 425–479Google Scholar
  168. Shin D-Y, Matsumoto K, Iida H, Uno 1, Ishikawa T (1987) Heat Shock Response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation. Mol Cell Biol 7: 244–250PubMedGoogle Scholar
  169. Slade L, Levine H (1988) Non-equilibrium behavior of small carbohydrate-water systems. Pure Appl Chem 60: 1841–1864CrossRefGoogle Scholar
  170. Smith SE (1967) Carbohydrate translocation in orchid mycorrhizas. New Phytol 66: 371–378CrossRefGoogle Scholar
  171. Stewart LC, Richtmeyer NK, Hudson CS (1950) The preparation of trehalose from yeast. J Am Chem Soc 72: 2059–2061CrossRefGoogle Scholar
  172. Strom AR, Falkenberg P, Landfald B (1986) Genetics of osmoregulation in Escherichia coli: uptake and biosynthesis of organic osmolytes. FEMS Microbiol Rev 39: 7986Google Scholar
  173. Sugihara TF, Kline L (1986) Factors affecting the stability of frozen bread doughs. II. Prepared by the sponge and dough method. Bakers Dig 42: 51–54, 69Google Scholar
  174. Sumida M, Ogura S, Miyata S, Arai M, Murao S (1989) Purification and some properties of trehalase from Chaetomium aureum MS — 27. J Ferment Bioeng 67: 8386CrossRefGoogle Scholar
  175. Suomalainen H, Pfäffii S (1961) Changes in the carbohydrate reserves of baker’s yeast during growth and on standing. J Inst Brew 67: 249–254Google Scholar
  176. Sussman AS (1954) Changes in the permeabilty of ascospores of Neurospora tetrasperma during germination. J Gen Physiol 38: 59–77PubMedCrossRefGoogle Scholar
  177. Sussman AS, Halvorson HO (1966) Spores. Their dor- mancy and germination. Harper & Row, New YorkGoogle Scholar
  178. Tanaka K, Matsumoto K, Toh-e A (1988) Dual regulation of the expression of the polyubiquitin gene by cyclic AMP and heat shock in yeast. EMBO J 7: 495–502PubMedGoogle Scholar
  179. Tereshina VM, Polotebnova MV, Feofilova EP (1988) Trehalase activity of spores of the wild-type strain of Cunninghamella japonica and mutants with a reduced rate of trehalase synthesis. Microbiology 56: 587–592Google Scholar
  180. Thevelein JM (1984a) Cyclic-AMP content and trehalase activation in vegetative cells and ascospores of yeast. Arch Microbiol 138: 64–67PubMedCrossRefGoogle Scholar
  181. Thevelein JM (1984b) Activation of trehalase by heat shock in yeast ascospores. Correlation with total cellular cyclic-AMP content. Curr Microbiol 10: 159–164CrossRefGoogle Scholar
  182. Thevelein JM (1984e) Regulation of trehalose mobilization in fungi. Microbiol Rev 48: 42–59PubMedGoogle Scholar
  183. Thevelein JM (1988) Regulation of trehalase activity by phosphorylation-dephosphorylation during developmental transitions in fungi. Exp Mycol 12: 1–12CrossRefGoogle Scholar
  184. Thevelein JM (1991) Fermentable sugars and intracellular acidification as specific activators of the RAS adenylate cyclase signalling pathway in yeast — the relationship to nutrient induced cell cycle control. Mol Microbiol 5: 1301–1307PubMedCrossRefGoogle Scholar
  185. Thevelein JM (1992) The RAS-adenylate cyclase pathway and cell cycle control in Saccharomyces cerevisiae. In: Grivell L (ed) Molecular biology of yeasts. Antonie Leeuwenhoek, J Microbiology 62: 109–130, Kluwer, DordrechtGoogle Scholar
  186. Thevelein JM, Beullens M (1985) Cyclic AMP and the stimulation of trehalase activity in the yeast Saccharomyces cerevisiae by carbon sources, nitrogen sources and inhibitors of protein synthesis. J Gen Microbiol 131: 3199–3209PubMedGoogle Scholar
  187. Thevelein JM, den Hollander JA, Shulman RG (1982) Changes in the activity and properties of trehalase during early germination of yeast ascospores: correlation with trehalose breakdown as studied by in vivo “C NMR. Proc Natl Acad Sci USA 79: 3503–3507PubMedCrossRefGoogle Scholar
  188. Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, Cameron S, Broach J, Matsumoto K, Wigler M (1985) In yeast, Ras proteins are controlling elements of adenylate cyclase. Cell 40: 27–36Google Scholar
  189. Tripp ML, Paznokas JL (1982) Glucose-initiated germination of Mucor racemosus sporangiospores. J Gen Microbiol 128: 477–483PubMedGoogle Scholar
  190. Trivedi NB, Jacobson G (1986) Recent advances in baker’s yeast. Prog Ind Microbiol 23: 45–71Google Scholar
  191. Uno I, Matsumoto K, Adachi K, Ishikawa T (1983) Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast. J Biol Chem 258: 10867–10872PubMedGoogle Scholar
  192. Van Aelst L, Boy-Marcotte E, Camonis JH. Thevelein JM, Jacquet M (1990) the C-terminal part of the CDC25 gene product plays a key role in signal transduction in the glucose-induced modulation of cAMP level in Saccharomyces cerevisiae. Eur J Biochem 193: 675–680Google Scholar
  193. Van Aelst L, Hohmann S, Zimmermann FK, Jans AWH, Thevelein JM (1991) A yeast homologue of the bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling. EMBO J 10: 2095–2104PubMedGoogle Scholar
  194. Van Aelst L, Hohmann S, Bulaya B, De Koning W, Sierkstra L, Neves MJ, Luyten K, Alijo R, Ramos J, Coccetti P, Martegani E, de Magalhäes-Rocha NM, Brandäo RL, Van Dijck P, Vanhalewyn M, Durnez P, Jans AWH, Thevelein JM (1993) Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae. Mol Microbial 8: 927–943CrossRefGoogle Scholar
  195. van de Poll KW, Schamhart DHJ (1977) Characterization of a regulatory mutant of fructose-l,6-diphosphatase in Saccharomyces carlsbergensis. Mol Gen Genet 154: 6166CrossRefGoogle Scholar
  196. Vandercammen A, François J, Hers H-G (1989) Characterization of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Saccharomyces cerevisiae. Eur J Biochem 182: 613–620PubMedCrossRefGoogle Scholar
  197. Van der Plaat JB (1974) Cyclic 3’,5’-adenosine monophosphate stimulates trehalose degradation in backers’ yeast. Biochem Biophys Res Commun 56: 580–587PubMedCrossRefGoogle Scholar
  198. Van Dijck P, Colarizzon D, Smet P. Theelein JM (1995) Differential importance of trehalose in stress resistance in fermenting and nonfermenting Saccharomyces cerevisiae cells. Appt Environm Microbiol 61: 109–115Google Scholar
  199. Van Doorn J, Scholte ME, Postma PW, van Driel R, van Dam K (1988a) Regulation of trehalase activity during the cell cycle of Saccharomyces cerevisiae. J Gen Microbiol 134: 785–790PubMedGoogle Scholar
  200. Van Doom J, Valkenburg JAC, Scholte ME, Oehlen LJ, van Driel R, Postma PW, Nanninga N, van Dam K (1988b) Changes in activities of several enzymes involved in carbohydrate metabolism during the cell cycle of Saccharomyces cerevisiae. J Bacterial 170: 4808–4815Google Scholar
  201. Vanhalewyn M, Thevelein JM (1992) Lcrl a mutation in the yeast adenylate cyclase gene. Yeast 8(Spec Iss):S391Google Scholar
  202. Van Laere AJ (1986a) Biochemistry of spore germination in Phycomyces. FEMS Microbiol Rev 32: 189–198Google Scholar
  203. Van Laere AJ (1986b) Resistance of germinating Phycomyces spores to desiccation, freezing, and acids. FEMS Microbiol Ecol 38: 251–256CrossRefGoogle Scholar
  204. Van Laere AJ (1989) Trehalose, reserve or stress metabolite. FEMS Microbiol Rev 63: 201–210Google Scholar
  205. Van Laere A, Slegers LK (1987) Trehalose breakdown in germinating spores of Mucor rouxii. FEMS Microbiol Lett 41: 247–252CrossRefGoogle Scholar
  206. Van Laere A, François A, Overloop K, Verbeke M, Van Gerven L (1987) Relation between germination, trehalose and the status of water in Phycomyces blakeslceanus spores as measured by proton-NMR. J Gen Microbiol 133: 239–245Google Scholar
  207. Van Mulders RM, Van Laere AJ (1984) Cyclic AMP, trehalase and germination of Phycomyces hlakesleeanus spores. J Gen Microbial 130: 541–547Google Scholar
  208. Vicente-Soler J, Arguelles JC, Gacto M (1989) Presence of two trehalose-6-phosphate synthase enzymes in Candida utilis. FEMS Microbial Lett 61: 273–278CrossRefGoogle Scholar
  209. Vicente-Soler J. Arguelles JC, Gacto M (1991) Proteolytic activation of a,a-trehalose 6-phosphate synthase in Candida utilis. FEMS Microbial Lett 82: 157–161Google Scholar
  210. Von Meyenburg HK (1969) Energetics of the budding cycle of Saccharomyces cerevisiae during glucose limited aerobic growth. Arch Mikrobiol 66: 289–303CrossRefGoogle Scholar
  211. Vuorio O, Londesborough J. Kalkkinen N (1992) Trehalose synthase: purification of the intact enzyme and cloning of the structural genes. Yeast 8 (Spec Iss): S626Google Scholar
  212. Walton EF, Carter BLA, Pringle JR (1979) An enrichment method for temperature-sensitive and auxotrophic mutants of yeast. Mol Gen Genet 171: 111–114CrossRefGoogle Scholar
  213. Werner-Washburne M, Becker J, Kosic-Smithers J, Craig EA (1989) Yeast Hsp70 RNA levels vary in response to the physiological status of the cell. J Bacterial 171: 26802688Google Scholar
  214. Wiemken A (1990) Trehalose in yeast, stress protectant rather than reserve carbohydrate. Anionic Leeuwenhock 58: 209–217CrossRefGoogle Scholar
  215. Wieser R, Adam G, Wagner A, Schuller C, Marchler G, Ruis H, Krawiec Z, Bilinski T (1991) Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase-T of Saccharomyces cerevisiae. J Biol Chem 266: 12406–12411PubMedGoogle Scholar
  216. Winkler K, Kienle I, Burgert M. Wagner JC, Holzer H (1991) Metabolic regulation of the trehalose content of vegetative yeast. FEBM Lett 291: 269–272CrossRefGoogle Scholar
  217. Yost HJ, Lindquist S (1991) Heat shock proteins affect RNA processing during the heat shock response of Saccharomyces cerevisiae. Mol Cell Biol 11: 1062–1068PubMedGoogle Scholar
  218. Zevenhuizen LPTM (1992) Levels of trehalose and glycogen in Athrohacterglohiformis under conditions of nutrient starvation and osmotic stress. Anfonie Leeuwenhoek 61: 61–68CrossRefGoogle Scholar
  219. Zikmanis PB, Laivenieks MG, Auzinya LP, Kulaev IS, Beker ME (1985) Relationship between the content of high-molecular-weight polyphosphates and trehalose and viability of populations following dehydratation of the yeast Saccharomyces cerevisiae. Microbiology 54: 326–330Google Scholar
  220. Zikmanis PB, Kruche RV, Auzinya LP, Margevicha MV, Becker E (1988) Distribution of trehalose between dehydrated Saccharomyces cerevisiae cells and the rehydratation medium. Microbiology 57: 414–416Google Scholar
  221. Zimmermann ALS, Terenzi HF, Jorge JA (1990) Purification and properties of an extracellular conidial trehalase from Humicola grisea Var Thermoidea. Biochim Biophys Acta 1036: 41–46PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • J. M. Thevelein
    • 1
  1. 1.Laboratorium voor Moleculaire CelbiologieKatholieke Universiteit LeuvenLeuven-Heverlee, FlandersBelgium

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