Fungal Protoplasts

  • J. F. Peberdy
Part of the The Mycota book series (MYCOTA, volume 2)

Abstract

In the 40 years since the first reported isolation of protoplasts from filamentous or mycelial fungi (Peberdy 1971), these structures have become central to research in several key areas of fungal biology. The switch in focus in genetics from the classical to a molecular approach is the major factor. Two genetic techniques, protoplast fusion and transformation, together with electrophoretic techniques to determine the molecular karyotype of fungi are all dependent on the effective use of protoplasts. As a consequence, other aspects of research that employ protoplasts have benefited more generally. At the beginning, protoplasts held high curiosity value but their scientific potential was not fully realized. However, as the desire to extend the protoplast isolation technique to more and more fungi has occurred, correspondingly more attention has been given to improving this essential process. One outcome has been an overall improvement in protoplast yields for many fungi, which in turn has reawakened the interest of researchers addressing cell biological, physiological, and biochemical questions and led to the adoption of protoplasts as experimental tools wherever relevant.

Keywords

Fermentation Polysaccharide Immobilization Carotenoid Alkaloid 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • J. F. Peberdy
    • 1
  1. 1.Department of BiologyChinese University of Hong KongShatin, New TerritoriesHong Kong

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