Mechanism and Control of Pre-mRNA Splicing
Most protein-encoding genes in eukaryotes are interrupted by intervening sequences that must be precisely removed to assure correct gene expression. The process by which these intervening sequences, the introns, are excised from a pre-mRNA and the flanking sequences, the exons, are joined to generate a functional mRNA is called pre-mRNA splicing. Pre-mRNA splicing is executed by a large ribonucleoprotein (RNP) machinery, the spliceosome, which consists of five small nuclear RNAs (snRNAs), U1, U2, U4, U5, and U6, and more than 80 proteins (Burge et al. 1999). The spliceosome is a highly dynamic complex, and extensive RNA-RNA and RNA-protein interactions are involved in the recognition of an intron and its removal from the pre-mRNA.
KeywordsCodon Catalysis Adenosine Serine Assure
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