Mechanism and Control of Pre-mRNA Splicing
Most protein-encoding genes in eukaryotes are interrupted by intervening sequences that must be precisely removed to assure correct gene expression. The process by which these intervening sequences, the introns, are excised from a pre-mRNA and the flanking sequences, the exons, are joined to generate a functional mRNA is called pre-mRNA splicing. Pre-mRNA splicing is executed by a large ribonucleoprotein (RNP) machinery, the spliceosome, which consists of five small nuclear RNAs (snRNAs), U1, U2, U4, U5, and U6, and more than 80 proteins (Burge et al. 1999). The spliceosome is a highly dynamic complex, and extensive RNA-RNA and RNA-protein interactions are involved in the recognition of an intron and its removal from the pre-mRNA.
KeywordsSplice Site Spinal Muscular Atrophy Fission Yeast Splice Factor Survival Motor Neuron
Unable to display preview. Download preview PDF.
- Burge CB, Tuschl TH, Sharp PA (1999) Splicing of precursors to mRNAs by the spliceosomes. In: Gesteland RF, Cech TR, Atkins JF (eds) RNA World II. CSH Laboratory Press, Cold Spring Harbor, pp 525–560Google Scholar
- Collins CA, Guthrie C (2000) The question remains: is the spliceosome a ribozyme? Nat Struct Biol 10: 850–854Google Scholar
- Rosenberg GH, Alahari SK, Käufer NF (1991) prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns. Mol Gen Genet 226: 305–309Google Scholar
- Schmidt H, Richert K, Drakas RA, Käufer NF (1999) spp42, identified as a classical suppressor of prp4–73, which encodes a kinase involved in pre-mRNA splicing in fission yeast, is a homologue of the splicing factor Prp8p. Genetics 153: 1183–1191Google Scholar
- Takeuchi M, Yanagida M (1993) A mitotic role for a novel fission yeast protein kinase dskl with cell cycle stage dependent phosphorylation and localization. Mol Biol Cell 4: 247260Google Scholar