The Fission Yeast Actomyosin Cytoskeleton
Everything interesting that happens to a fission yeast happens at the ends of the cell or in the middle. Cell growth and conjugation are confined to the cell poles whilst cell division, both mitosis and cytokinesis, occurs at the equator. More than 15 years ago we showed that the distribution of actin through the fission yeast cell cycle precisely paralleled the pattern of cell growth and cytokinesis (Marks et al. 1986). Here, we review the progress that has been made in our understanding of the role of the actin cytoskeleton since these initial observations and, in particular, the role of the myosin gene family.
KeywordsFission Yeast Myosin Light Chain Kinase Schizosaccharomyces Pombe Actin Cable Contractile Ring
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- Botstein D, Amberg D, Mulholland J et al. (1997). The yeast cytoskeleton. In: Pringle JR, Broach JR, Jones EW (eds) The molecular and cellular biology of the yeast Saccharomyces: cell cycle and cell biology. CSH Laboratory Press, Cold Spring Harbor, pp 1–90Google Scholar
- Hallett MA, Lo HS, Bender A (2002) Probing the importance and potential roles of the binding of the PH-domain protein Boil to acidic phospholipids. BMC Cell Biol 3:16 Hodge T, Cope MJTV. (2000) A myosin family tree. J Cell Sci 113: 3353–3354Google Scholar
- May KM, Wheatley SP, Amin V, Hyams JS (1998 a) The myosin ATPase inhibitor 2,3-butanedione-2-monoxime (BDM) inhibits tip growth and cytokinesis in the fission yeast, Schizosaccharomyces pombe. Cell Motil Cytoskel 41: 117–125Google Scholar
- McCollum D, Balasubramanian M, Pelcher LE., Hemmingsen et al. (1995) Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis. J Cell Biol 130: 651–660Google Scholar
- McCollum D, Balasubramanian M, Gould K (1999 a) Identification of cold-sensitive mutations in the Schizosaccharomyces pombe actin locus. FEBS Lett 451: 321–326Google Scholar
- Mulvihill DP, Hyams JS (2003) Role of the two type II myosins, Myo2 and Myp2 in cytokinetic actomyosin ring formation and function in fission yeast. Cell Motil Cytoskel (in press)Google Scholar
- Nakano K, Satoh K, Morimatsu A et al. (2001) Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton. Mol Biol Cell 11: 3515–3526Google Scholar
- Verde F, Wiley DJ, Nurse P (1998) Fission yeast orb6, a ser/thr protein kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle. Proc Natl Acad Sci USA 95: 7526–7531PubMedCrossRefGoogle Scholar