Growth of Nerve Fibers to Merkel Cells Observed in Co-Culture of Sensory Ganglia and Sinus Hair Follicles

  • Jun Fukuda
  • Hisako Ishimine
  • Kazuko Keino-Masu
  • Yoshiaki Masaki
Conference paper

Summary

Merkel cells have long been assumed to exhibit a function of guiding sensory nerve fibers to the skin and hairs. However, there has been little experimental evidence that supports this hypothesis. In order to test this possibility, we performed co-culture of sinus hair follicles and sensory ganglia in a collagen gel, intending to examine how sensory nerve fibers grow to the Merkel cell-enriched regions of sinus hair follicles. During co-culture with a serum-free medium supplemented with NGF and NT3, nerve fibers from sensory ganglia grew in a manner making convergence to the superior enlargement of hair follicles at which Merkel cells were enriched. Live Merkel cells stained with FM1-43 dye were demonstrated to be present in co-culture preparations. The presence of Merkel cells in this region of hair follicles was also demonstrated by immunostaining against cytokeratin 20. Immunostaining of a co-culture preparation against synaptophysin 1 showed that the hair follicle was surrounded tightly with synaptophysin 1-enriched newly grown sensory nerve fibers. We therefore considered that Merkel cells are likely to have a capability of attracting sensory nerve fibers.

Keywords

Progesterone Paraformaldehyde Putrescine 

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References

  1. Andres KH (1966) Über die Feinstruktur der Rezeptoren an Sinushaaren. Z Zellforsch 75: 339–365PubMedCrossRefGoogle Scholar
  2. Fukuda J (1996) A pure, monolayer culture of Merkel cells from sinus hair follicles of the rat. Neurosci Lett 216: 73–76PubMedCrossRefGoogle Scholar
  3. Lumsden AG, Davies AM (1986) Chemotropic effect of specific target epithelium in the developing mammalian nervous system. Nature 323: 538–539PubMedCrossRefGoogle Scholar
  4. Ryan TA (2001) Presynaptic imaging techniques. Curr Opin Neurobiol 11:544–549 Sudhof TC (2000) The synaptic vesicle cycle revisited. Neuron 28: 317–320Google Scholar
  5. Tachibana T, Fujiwara N, Nawa T (2000) Postnatal differentiation of Merkel cells in the rat palatine mucosa, with special reference to the timing of peripheral nerve development and the potency of cell mitosis. Anat Embryol 202: 359–367PubMedCrossRefGoogle Scholar
  6. Vos P, Stark F, Pittman RN (1991) Merkel cells in vitro: production of nerve growth factor and selective interactions with sensory neurons. Dev Biol 144: 281–300PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Jun Fukuda
    • 1
  • Hisako Ishimine
    • 1
  • Kazuko Keino-Masu
    • 1
  • Yoshiaki Masaki
    • 1
  1. 1.Laboratory of Molecular and Cellular Physiology, Department of PhysiologyNational Defense Medical College of JapanTokorozawa, SaitamaJapan

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