Regeneration of Plants from Protoplasts of Helianthus annuus L. (Sunflower)

  • C. Fischer
  • H. Laparra
  • F. Charrière
  • J.-L. Jung
  • G. Hahne
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 38)

Abstract

Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.) has met with considerable difficulties in spite of numerous efforts over several years. The isolation of protoplasts and their culture up to the callus stage does not appear to present major difficulties (Bohorova et al. 1986; Lenée and Chupeau 1986; Moyne et al. 1988; Chanabé et al. 1989; Guilley and Hahne 1989; Schmitz and Schnabl 1989; Dupuis et al. 1990). Most currently used donor materials, including hypocotyl, cotyledons, petioles, leaves, and suspension cultures, allow the isolation of protoplasts at comparatively high yields, and media have been developed for the regeneration of callus with good plating efficiencies from all of these types.

Keywords

Migration Sucrose Agarose Glutamine Cellulase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barth S, Voeste D, Schnabl H (1993) Somatic hybrids of sunflower (Helianthus annuus L.) identified at the callus stage by isoenzyme analysis. Bot Acta 106: 100–102Google Scholar
  2. Binding H, Nehls R, Kock R, Finger J, Mordhorst G (1981) Comparative study on protoplast regeneration in herbaceous species of the Dicotyledoneae class. Z Pflanzenphysiol 101: 119–130Google Scholar
  3. Bohorova NE, Cocking EC, Power JB (1986) Isolation, culture and callus regeneration of proto-plasts of wild and cultivated Helianthus species. Plant Cell Rep 5: 256–258CrossRefGoogle Scholar
  4. Burrus M, Chanabé C, Alibert G, Bidney D (1991) Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.). Plant Cell Rep 10: 161–166CrossRefGoogle Scholar
  5. Carter JF (1978) Sunflower science and technology. Am Soc Agron, Crop Sci Soc Am, Soil Sci Soc Am MadisonGoogle Scholar
  6. Chanabé C, Burrus M, Alibert G (1989) Factors affecting the improvement of colony formation from sunflower protoplasts. Plant Sci 64: 125–132CrossRefGoogle Scholar
  7. Chanabé C, Burrus M, Bidney D, Alibert G (1991) Studies on plant regeneration from protoplasts in the genus Helianthus. Plant Cell Rep 9: 635–638CrossRefGoogle Scholar
  8. Dupuis JM, Péan M, Chagvardieff P (1990) Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L. Plant Cell Tissue Organ Cult 22: 183–189CrossRefGoogle Scholar
  9. Fischer C, Hahne G (1992) Structural analysis of colonies derived from sunflower (Helianthus annuus L.) protoplasts cultured in liquid and semisolid media. Protoplasma 169: 130–138CrossRefGoogle Scholar
  10. Fischer C, Klethi P, Hahne G (1992) Protoplasts from cotyledon and hypocotyl of sunflower (Helianthus annuus L.): shoot regeneration and seed production. Plant Cell Rep 11: 632–636CrossRefGoogle Scholar
  11. Fischer C, Laparra H, Hahne G (1993) Towards somatic hybrids of sunflower. Biotechnol Biotechnol Equip 7: 129–131Google Scholar
  12. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158PubMedCrossRefGoogle Scholar
  13. Gavin AL, Conger BV, Trigano RN (1989) Sexual transmission of somatic embryogenesis in Dactylis glomerata. Plant Breed 103: 251–254CrossRefGoogle Scholar
  14. Georgieva-Todorova J (1976) Interspecific relationships within the genus Helianthus. Bulg Acad Sci, SofiaGoogle Scholar
  15. Guilley E, Hahne G (1989) Callus formation from isolated sunflower (Helianthus annuus) mesophyll protoplasts. Plant Cell Rep 8: 226–229CrossRefGoogle Scholar
  16. Kahl G, Kaemmer D, Weising K, Kost S, Weigand F, Saxena MC (1994) The potential of gene technology and genome analysis for cool season food legume crops: theory and practice. Euphytica 73: 177–189CrossRefGoogle Scholar
  17. Kirches E, Frey N, Schnabl H (1991) Transient gene expression in sunflower mesophyll protoplasts. Bot Acta 104: 212–216Google Scholar
  18. Krasnyanski S, Menczel L (1993) Somatic embryogenesis and plant regeneration from hypocotyl protoplasts of sunflower (Helianthus annuus L.). Plant Cell Rep 12: 260–263CrossRefGoogle Scholar
  19. Krasnyanski S, Menczel L (1995) Production of fertile somatic hybrid plants of sunflower and Helianthus giganteus L. by protoplasts fusion. Plant Cell Rep 14: 232–235CrossRefGoogle Scholar
  20. Krasnyanski S, Polgar Z, Németh G, Menczel L (1992) Plant regeneration from callus and protoplast cultures of Helianthus giganteus L. Plant Cell Rep 11: 7–10CrossRefGoogle Scholar
  21. Laparra H, Burrus M, Hunold R, Damm B, Bravo-Angel AM, Bronner R, Hahne G (1995) Expression of foreign genes in sunflower (Helianthus annuus L.) — evaluation of three transfer methods. Euphytica 85: 63–74CrossRefGoogle Scholar
  22. Lenée P, Chupeau Y (1986) Isolation and culture of sunflower protoplasts (Helianthus annuus L.): factors influencing the viability of cell colonies derived from protoplasts. Plant Sci 43: 69–75CrossRefGoogle Scholar
  23. Moyne AL, Thor V, Pelissier B, Bergounioux C, Freyssinet G, Gadal P (1988) Callus and embryoid formation from protoplasts of Helianthus annuus. Plant Cell Rep 7: 437–440Google Scholar
  24. Moyne AL, Tagu D, Thor V, Bergounioux C, Freyssinet G, Gadal P (1989) Transformed calli obtained by direct gene transfer into sunflower protoplasts. Plant Cell Rep 8: 97–100CrossRefGoogle Scholar
  25. Murashige T, Skoog F (1962) A revised medium for growth and rapid bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  26. Polgar Z, Krasnyanski S (1992) Plant regeneration from cell suspension and mesophyll protoplasts of Helianthus maximiliani ( Schrad. ). Plant Sci 87: 191–197CrossRefGoogle Scholar
  27. Ray JM, Bingham ET (1989) Breeding alfalfa for regeneration from tissue culture. Crop Sci 29: 1545–1548CrossRefGoogle Scholar
  28. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199–204CrossRefGoogle Scholar
  29. Schilling EE, Heiser CB (1981) Intrageneric classification of Helianthus Compositae. Taxon 30: 393–401CrossRefGoogle Scholar
  30. Schmitz P, Schnabl H (1989) Regeneration and evacuolation of protoplasts from mesophyll, hypocotyl, and petioles from Helianthus annuus L. J Plant Physiol 135: 223–227CrossRefGoogle Scholar
  31. Sundberg E, Glimelius K (1986) A method for production of interspecific hybrids within Brassicaceae via somatic hybridization using resynthesis of Brassica napus as a model. Plant Sci 43: 155–162CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • C. Fischer
    • 2
  • H. Laparra
    • 1
  • F. Charrière
    • 1
  • J.-L. Jung
    • 3
  • G. Hahne
    • 1
  1. 1.Institut de Biologie Moléculaire des PlantesCNRS et Université Louis PasteurStrasbourg CedexFrance
  2. 2.Institut für Biologie II/IIIAlbert-Ludwigs-Universität FreiburgFreiburgGermany
  3. 3.Technopole Brest IroiseISAMORPlouzané, BrestFrance

Personalised recommendations