Abstract
The genus Carica is a native to tropical and subtropical America and comprises about 40 species, of which Carica papaya L. (papaya) is the only one being widely cultivated. Papaya is believed to have arisen from C. peltata Hook. & Arn. by natural hybridization. It is an important crop, producing edible fruit and papain-containing latex. The flavorful, melon-like fruit contains abundant vitamin A and some vitamin C. It is a popular dessert fruit used for making jams, preserves, and ice cream flavoring; it is also served as a vegetable. The laticifers occur in all parts of the plant. The dried latex from unripe fruit is the source of papain, a proteolytic enzyme which is widely used as a meat tenderizer and in the tanning, cosmetics, and pharmaceutical industries (Litz 1984, 1986).
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References
Chen MH, Chen CC (1992) Plant regeneration from Carica protoplasts. Plant Cell Rep 11: 404–407
Chen MH, Chen CC, Wang DN, Chen FC (1991) Somatic embryogenesis and plant regeneration from immature embryos of Carica papaya x Carica cauliflora cultured in vitro. Can J Bot 69: 1913–1918
Conover RA (1964) Distortion ringspot, a severe virus disease of papaya in Florida. Proc Fla State Hortic Soc 77: 440–444
Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC (1990) Stable transformation of papaya via microprojectile bombardment. Plant Cell Rep 9: 189–194
Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC (1992) Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus. Bio/Technology 10: 1466–1472
Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL (1993) Transgenic papaya plants from Agrobacterium-mediated transformation of somatic embryos. Plant Cell Rep 12: 245–249
Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33: 130–137
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50: 151–158
Jordan M, Ciudad G, Rojas ML, Valverde F (1986) Isolation, culture and fusion of Carica candamarcensis and C. papaya protoplasts. Gartenbauwissenschaft 51: 175–178
Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110
Kao KN, Constabel F, Michayluk MR, Gamborg OL (1974) Plant protoplast fusion and growth of intergeneric hybrid cells. Planta 120: 215–227
Khuspe SS, Hendre SS, Mascarenhas AF, Jagannathan V (1980) Utilization of tissue culture to isolate interspecific hybrids in Carica L. In: Rao PS, Heble MR, Chadha MS (eds) Plant tissue culture, genetic manipulation and somatic hybridization of plant cells. Bhabha Atomic Res Center, Bombay, India, pp 189–205
Litz RE (1984) Papaya. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 2. Macmillan, New York, pp 349–368
Litz RE (1986) Papaya (Carica papaya L.) In: Bajaj YPS (eds) Biotechnology in agriculture and forestry, vol 1. Trees I. Springer Berlin Heidelberg, pp 220–232
Litz RE, Conover RA (1979) Development of systems for obtaining parasexual Carica hybrids. Proc Fla State Hortic Soc 92: 281–283
Litz RE, Conover RA (1980) Somatic embryogenesis in cell cultures of Carica stipulata. HortScience 15: 733–735
Liu CS, Chow HT, Chen CY (1984) Isolation and culture of papaya protoplasts - studies on cultural environments and media supplements. J Sci Eng (Chung Hsing University, Taichung, Taiwan ) 21: 89–98
Manshardt RM. Wenslaff TF (1989) Interspecific hybridization of papaya with other Carica species. J Am Soc Hortic Sci 114: 689–694
Mekako HU, Nakasone HY (1975) Interspecific hybridization among 6 Carica species. J Am Soc Hortic Sci 100: 237–242
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87
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© 1994 Springer-Verlag Berlin Heidelberg
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Chen, M.H. (1994). Regeneration of Plants from Protoplasts of Carica Species (Papaya). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering V. Biotechnology in Agriculture and Forestry, vol 29. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09366-5_4
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DOI: https://doi.org/10.1007/978-3-662-09366-5_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08182-8
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