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Genetic Transformation in Larix Species (Larches)

  • Chapter
Plant Protoplasts and Genetic Engineering V

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 29))

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Abstract

Larch has advantages as a model system in the application of genetic engineering to conifers. First, it is relatively easy to regenerate plants from various sources in vitro in larches. Since the first report on organogenesis from callus cultures of Larix by Momot (1976), organogenesis from juvenile tissues (Bonga and McInnis 1983; Bonga 1984; Karnosky and Diner 1984) and mature tissues (Bonga 1984), and embryogenesis from gametophyte tissue (Nagmani and Bonga 1985), immature embryos (Von Aderkas et al. 1990), and protoplast cultures (Klimaszewska 1989) have been reported. Because reliable regeneration systems from cells or tissue cultures are an essential prerequisite for genetic transformation in both direct and indirect gene transfer methods, established regeneration systems (Karnosky 1992) in larch make this species a promising candidate for genetic transformation in conifers. Second, while the host range of Agrobacterium has been limited with regard to conifers, larch is susceptible to Agrobacterium (Diner and Karnosky 1987; Huang et al. 1991). Thus, the prerequisites for successful transformation have been established in larch.

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References

  • Adang MJ, Stayer MJ, Rochelean TA, Leighton J, Barker RF, Thompson DV (1985) Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thrungiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta. Gene 36: 289–300

    Article  PubMed  CAS  Google Scholar 

  • Aono M, Kubo A, Saji H, Natori T, Tanaka K, Kondo N (1991) Resistance to active oxygen toxicity of transgenic Nicotiana tabacum that expresses the gene for glutathione reductase from Escherichia coli. Plant Cell Physiol 32: 691–697

    CAS  Google Scholar 

  • Baker SS, Rush CL, Kamalay JC (1990) RNA and DNA isolation from recalcitrant plant tissues. Biotechniques 9 (3): 268–272

    PubMed  CAS  Google Scholar 

  • Bauer EU, Michler CH (1989) Microspray applicator and enclosure for administering chemicals to plants. HortScience 24 (4): 704

    Google Scholar 

  • Bercetche J, Chriqui D, Adams S, David C (1987) Morphogenic and cellular reorientation induced by Agrobacterium rhizogenes (strain 1855, 2659, and 8196) on carrot, pea, and tobacco. Plant Sci 52: 195–210

    Google Scholar 

  • Bonga JM (1984) Adventitious shoot and root formation in tissue cultures of mature Larix decidua. In: Hanover J, Karnosky DF, Keathley D (eds) Proc Int Symp Recent advances in forest biotechnology, Traverse City, Michigan., Michigan Biotechnology Institute, East Lansing, pp 64–68

    Google Scholar 

  • Bonga JM, Mclnnis AH (1983) Origin and early development of roots in plantlets derived from embryo sections of Larix laricina in vitro. Can For Sery Res Notes 3 (2): 12–14

    Google Scholar 

  • Bugos RC, Chiang VC, Campbell WH (1991) cDNA cloning, sequence analysis and seasonal expression of lignin-bispecific caffeic acid/5-hydroxyferulic acid 0-methyltransferase of aspen. Plant Mol Biol 17: 1203–1215

    Google Scholar 

  • Campbell AJ (1983) Nursery practice and early establishment of larch. In: Proc Larch Symp Potential for the future. Univ Toronto, pp 11–26

    Google Scholar 

  • Carozzi NB, Warren GW, Desai N, Jayne SM, Lotstein R, Rice DA, Evola S, Koziel MG (1992) Expression of a chimeric CaMV 35S Bacillus thrungiensis insecticidal protein gene in transgenic tobacco. Plant Mol Biol 20: 539–548

    Article  PubMed  CAS  Google Scholar 

  • Charest PJ, Devantier Y, Ward C, Jones C, Schaffer U, Klimaszewska KK (1991) Transient expression of foreign chimeric genes in the gymnosperm hybrid larch following electroporation. Can J Bot 69: 1731–1736

    Article  Google Scholar 

  • Charest PJ, Calero N, Lachance D, Datla RSS, Duchesne LC, Tsang EWT (1993) MicroprojectileDNA delivery in conifer species: factors affecting assessment of transient gene expression using the ß-glucuronidase reported gene. Plant Cell Rep 12: 189–193

    Article  CAS  Google Scholar 

  • Cheng J, Bolyard MG, Saxena RC, Sticklen MB (1992) Production of insect resistant potato by genetic transformation with b-endotoxin gene from Bacillus thrungiensis var. kurstaki. Plant Sci 81: 83–91

    Article  CAS  Google Scholar 

  • Chiang VL, Mroz GD, Shaler SM, Reed DD (1988) Pulp production of a I6-year-old larch stand. TAPPI J 71: 179–181

    CAS  Google Scholar 

  • Chilton MD, Currier TC, Farrand SK, Bendich AJ, Gordon MP (1974) Agrobacterium tume/àciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci USA 71: 3672–3676

    Google Scholar 

  • De Cleene M, De Ley J (1976) The host range of crown gall. Bot Rev 42: 389–446

    Article  Google Scholar 

  • Diner AM, Karnosky DF (1986) Agrobacterium: potential for genetic engineering. In: proc TAPPI Res Dev Conf, TAPPI, Technology Park, Atlanta, GA, pp 93–94

    Google Scholar 

  • Diner AM, Karnosky DF (1987) Differential responses of two conifers to in vitro inoculation with Agrobacterium rhizogenes. Eur J For Pathol 17: 211–216

    Article  Google Scholar 

  • Duchesne LC, Charest PJ (1992) Effect of promoter sequence on transient expression of the ß-glucuronidase gene in embryogenic calli of Larix x eurolepis and Picea mariana following microprojection. Can J Bot 70: 175–180

    Article  CAS  Google Scholar 

  • Einspahr DW, Wyckoff GW, Fiscus MH (1984) Larch — a fast growing fiber source for the Lake States and Northeast. J For 82: 104–106

    Google Scholar 

  • Fillatti JJ, Kisser J, Rose R, Cornai L (1987) Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Bio/Technology 5: 726–730

    Article  CAS  Google Scholar 

  • Fischhoff DA, Bowdish KS, Perlak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean DA, Kusano-Kretzner K, Mayer EJ, Rochester DE, Rogers SG, Fraley RT (1987) Insect tolerant transgenic tomato plants. Bio/Technology 5: 807–813

    Article  CAS  Google Scholar 

  • Fridovich 1 (1986) Biological effects of superoxide radical. Arch Biochem Biophys 247: 1–11

    Article  PubMed  CAS  Google Scholar 

  • Ge AT, Shivarova NI, Dean DH (1986) Location of the Bombyx mori specialty domain on a Bacillus thrungiensis-endotoxin protein. Proc Natl Acad Sci USA 86: 4037–4041

    Article  Google Scholar 

  • Gref R, Eriksson A (1985) Wound-induced changes of resin-acid concentrations in living bark of Scots pine seedlings. Can J For Res 15: 92–96

    Article  CAS  Google Scholar 

  • Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107: 161–170

    Article  Google Scholar 

  • Guerche P, Jouanin L, Tepfer D, Pelletier G (1987) Genetic transformation of oilseed rape (Brassica napus) by the Ri T-DNA of Agrobacterium rhizogenes and analysis of inheritance of the transformed phenotype. Mol Gen Genet 206: 382–386

    Article  CAS  Google Scholar 

  • Gupta PK, Durzan DJ (1985) Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep 4: 177–179

    Article  CAS  Google Scholar 

  • Hall JP, Brown JR (1977) Embryo development and seed yield of Larix in northeast Scotland. Silv Genet 25: 132–137

    Google Scholar 

  • Hausladen A, Madamanchi NR, Fellows S, Alscher RG, Amdson RG (1990) Seasonal changes in antioxidants in red spruce as affected by ozone. New Phytol 115: 447–458

    Article  CAS  Google Scholar 

  • Hobbs SLA, Kpodar P, DelongCMO (1990) The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol 15: 851–864

    CAS  Google Scholar 

  • Huang Y, Diner AM, Karnosky DF (1991) Agrobacterium rhizogenes-mediated genetic transformation and regeneration of a conifer: Larix decidua. In Vitro Cell Dev Biol 27:201–207

    Google Scholar 

  • Jones JDJ, Dunsmuir P, Bedbrook J (1985) High level expression of introduced chimeric genes in regenerated transformed plants. EMBO J 4: 2411–2418

    PubMed  CAS  Google Scholar 

  • Karnosky DF (1992) Micropropagation of larches (Larix spp.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 18. High-tech and micropropagation II. Springer, Berlin Heidelberg New York, pp 123–135

    Google Scholar 

  • Karnosky DF, Diner AM (1984) A cotyledon culture system for cloning Larix decidua and Pinus banksiana. In: Proc TAPPI Res Dev Conf. TAPPI, Technology Park, Atlanta, GA, pp 13–15

    Google Scholar 

  • Karnosky DF, Diner AM, Barnes WM (1988) A model system for gene transfer in conifers: European larch and Agrobacterium. In: Ahuja MR (ed) Somatic cell genetics of woody plants. Kluwer, Dordrecht, pp 55–63

    Chapter  Google Scholar 

  • Klimaszewska KK (1989) Recovery of somatic embryos and plantlets from protoplast cultures of Larix x eurolepis. Plant Cell Rep 8: 440–444

    Article  Google Scholar 

  • Litvay JD, Johnson MA, Verma D, Einspahr D, Weyrauch K (1981) Conifer suspension culture medium development using analytical data from developing seeds. Inst Pap Chem Tech Pap Ser, Appleton, WI, No 115: 1–17

    Google Scholar 

  • Momot TS (1976) Organogenesis of leaf tissues of Larix dahurica Turez, cultured in vitro. Lesnop Zh 5: 27–29

    Google Scholar 

  • Nagmani R, Bonga JM (1985) Embryogenesis in subcultured callus of Larix decidua. Can J For Res 15: 1088–1091

    Article  Google Scholar 

  • Netzer DA (1984) Herbicide trials on European larch in northern Wisconsin. USDA For Sery Res Note NC-318, 2 pp

    Google Scholar 

  • Noda T, Tanaka N, Mano Y (1987) Regeneration of horseradish hairy roots incited by Agrobacterium rhizogenes infection. Plant Cell Rep 6: 283–286

    Article  Google Scholar 

  • Palmer CL (1991) Short-rotation culture of Populus and Larix: a literature review. Canada-Ontario For Resource Dev Agreement Rep 3306, 65 pp

    Google Scholar 

  • Perlak FJ, Deaton RW, Armstrong TA, Fuchs RL, Sims SR, Greenplate JT, Fischhoff DA (1990) Insect resistant cotton plants. Bio/Technology 8: 939–943

    Article  PubMed  CAS  Google Scholar 

  • Riemenschneider DE, Haissig BE, Sellmer J, Fillatti JJ (1988) Expression of an herbicide tolerance gene in young plants of a transgenic hybrid poplar clone. In: Ahuja MR (ed) Somatic cell genetics of woody plants. Kluwer, Dordrecht, pp 73–80

    Chapter  Google Scholar 

  • Robbins K (1985) Risks associated with growing non-native larches in eastern North America. Northern J Appl For 2: 101–104

    Google Scholar 

  • Ryan RB, Tunnock S, Ebel FW (1987) The larch casebearer in North America. J For 85: 33–39

    Google Scholar 

  • Schnepf H, Whiteley HR (1985) Deleneation of a toxin-encoding segment of a Bacillus thrungiensis crystal protein gene. J Biol Chem 260 (10): 6273–6280

    PubMed  CAS  Google Scholar 

  • Scioli JR, Zilinskas BA (1988) Cloning and characterization of a cDNA encoding the chloroplastic copper/zinc-superoxide dismutase from pea. Proc Natl Acad Sci USA 85: 7661–7665

    Article  PubMed  CAS  Google Scholar 

  • Shah DM, Horsch RB, Klee HJ, Kishore GM, Winter JA, Turner NE, Horonaka CM, Sanders PR, Gasser CS, Aykent S, Siegel NR, Rogers SG, Fraley RT (1986) Engineering herbicide tolerance in transgenic plants. Science 233: 478–481

    Article  PubMed  CAS  Google Scholar 

  • Shibano Y, Yamagata A, Nakamura N, Iizuka T, Sugisaki H, Takanami M (1985) Nucleotide sequence coding for the insecticidal fragment of the Bacillus thrungiensis crystal protein. Gene 34: 243–251

    Article  PubMed  CAS  Google Scholar 

  • Shin D, Karnosky DF (1993) Factors affecting seed yields of Larix in Michigan’s Upper Peninsula. Proc Symp Ecology and management of Larix forests: a look ahead, Whitefish, MT. Intermountain Research Station, Forest Service, US Department of Agriculture (in press)

    Google Scholar 

  • Shin D, Huang Y. Podila GK, Karnosky DF (1994) Transgenic larch expressing genes for herbicide and insect resistance. Can J For Res (in press)

    Google Scholar 

  • Stalker DM, Hiatt WR, Cornai L (1985) A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. J Biol Chem 206 (8): 4724–4728

    Google Scholar 

  • Tepperman J, Katayama C, Dunsmuir P (1988) Cloning and nucleotide sequence of a petunia gene encoding a chloroplast localized superoxide dismutase. Plant Mol Biol 11: 871–872

    Article  CAS  Google Scholar 

  • Vaeck M, Reynaerts A, Hofte H, Jansens S, De Beuckeleer M, Dean C, Zabeau M, Van Montagu M, Leemans J (1987) Transgenic plants protected from insect attack. Nature 328: 33–37

    Article  CAS  Google Scholar 

  • Von Aderkas P, Klimaszewska KK, Bonga JM (1990) Diploid and haploid embryogenesis in Larix leptolepis, L. decidua and their reciprocal hybrids. Can J For Res 20: 9–14

    Article  Google Scholar 

  • Wessing K, Schell J, Kahl G (1988) Foreign genes in plants: transfer, structure, expression, and applications. Annu Rev Genet 22: 421–477

    Article  Google Scholar 

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Authors and Affiliations

  1. School of Forestry and Wood Products, Michigan Technological University, Houghton, Michigan, 49931, USA

    D. Shin & D. F. Karnosky

  2. Department of Biological Sciences, Michigan Technological University, Houghton, Michigan, 49931-1295, USA

    G. K. Podila

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  1. D. Shin
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  2. G. K. Podila
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  3. D. F. Karnosky
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Editors and Affiliations

  1. A-137, New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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© 1994 Springer-Verlag Berlin Heidelberg

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Shin, D., Podila, G.K., Karnosky, D.F. (1994). Genetic Transformation in Larix Species (Larches). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering V. Biotechnology in Agriculture and Forestry, vol 29. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09366-5_22

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  • DOI: https://doi.org/10.1007/978-3-662-09366-5_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08182-8

  • Online ISBN: 978-3-662-09366-5

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