Regeneration of Plants from Protoplasts of Triticum aestivum L. (Wheat)

  • Y-F. Chang
  • J. R. Wong
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 29)

Abstract

Wheat is one of the most important cereal crops in the world. It is grown in a wide range of environments over an area of 220 million ha with a production of about 564 million metric tons (FAO 1993). The leading wheat producing regions are the former USSR, China, USA, Canada, India, and Europe. Most of the wheat production is consumed as flour, with bread wheat (Triticum aestivum) accounting for approximately 80% of total consumption and durum wheat (Triticum durum) accounting for the remainder. For developing high-yield, high-nutrition, and disease-resistant varieties biotechnological techniques are needed to complement the traditional breeding methods in enlarging wheat genetic variability. An excellent review of how biotechnology may contribute in facilita­ting wheat breeding is given in Biotechnology in Agriculture and Forestry, Volume 13 (see Bajaj 1990). Literature on wheat protoplasts is summarized in Tables 1 and 2, and discussed below.

Keywords

Sucrose Photosynthesis Glutamine Lime Mannitol 

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References

  1. Ahmed KZ, Sagi F (1993) Culture of and fertile plant regeneration from regenerable embryogenic suspension cell-derived protoplasts of wheat (Triticum aestivum L.). Plant Cell Rep 12: 175–179CrossRefGoogle Scholar
  2. Bajaj YPS (ed) (1990) Biotechnology in agriculture and forestry, vol 13. Wheat, Springer, Berlin Heidelberg New YorkGoogle Scholar
  3. Bajaj YPS, Davey MR (1974) The isolation and ultrastructure of pollen protoplasts. In: Linskens HF (ed) Fertilization in higher plants. Elsevier, North Holland, Amsterdam, pp 73–80Google Scholar
  4. Bajaj YPS (1977) In vitro induction of haploids in wheat (Triticum aestivum L.). Crop Improv 4: 54–64Google Scholar
  5. Bajaj YPS (1983) Survival of somatic hybrid protoplasts of wheat x pea and rice x pea subjected to — 196 °C. Indian J Exp Biol 21: 120–122Google Scholar
  6. Brown C, Gilmour DM, Mathias RJ, Flavell RB (1988) Transient expression of foreign genes in wheat protoplasts. Plant Cell Tissue Organ Cult 12: 371–372Google Scholar
  7. Chang YF, Wang WC, Warfield CY, Nguyen TN, Wong J (1991a) Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.). Plant Cell Rep 9: 611–614CrossRefGoogle Scholar
  8. Chang YF, Wach M, Ulrich T, Nguyen T, Warfield C, Wang W, Wong J (1991b) Protoplast regeneration and transformation of wheat (Triticum aestivum L. cv. Mustang). Physiol Plan 82(1): A31Google Scholar
  9. Dudits D, Nemét G (1976) Methods of somatic plant genetics in cereal research. In: Semaine d’étude cereal culture. Centre de Recherche agronomique, Gembloux, Belgium, pp 127–139Google Scholar
  10. Edwards GE, Robinson SP, Tyler NJC, Walker DA (1978) Photosynthesis by isolated protoplasts, protoplast extracts, and chloroplasts of wheat —influence of orthophosphate, pyrophosphate, and adenylates. Plant Physiol 62 (2): 313–319PubMedCrossRefGoogle Scholar
  11. Evans PK, Keates AG, Cocking EC (1972) Isolation of protoplasts from cereal leaves. Planta 104: 178–181CrossRefGoogle Scholar
  12. FAO (1993) Quarterly bulletin of statistics 6(2):31. FAO, United Nations, RomeGoogle Scholar
  13. Frearson EM, Power JB Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33: 130–137Google Scholar
  14. Harms CT, Potrykus I (1978) Fractionation of plant protoplast type by iso-osmotic density gradient centrifugation. Theor Appl Genet 53: 57–63Google Scholar
  15. Harris H, Wright M, Byrne M, Varnum J, Brightwell B, Schubert K (1988) Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivum L.). Plant Cell Rep 7: 337–340CrossRefGoogle Scholar
  16. Hauptmann RM, Ozias-Akins P, Vasil V, Tabaeizadeh Z, Rogers SG, Horsch RB, Vasil IK, Fraley RT (1987) Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species. Plant Cell Rep 6: 265–270CrossRefGoogle Scholar
  17. Hayashi Y, Shimamoto K (1988) Wheat protoplast culture: embryogenic colony formation from protoplasts. Plant Cell Rep 7: 414–417CrossRefGoogle Scholar
  18. He DG, Yang YM, Scott KJ (1992) Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog). Plant Cell Rep 11: 16–19Google Scholar
  19. Jutori H, Misawa N, Ohyama K, Komano T (1983) Protoplast isolation from soybean and wheat cell suspension cultures and soybean-wheat intergeneric fusion. Mem Coll Agric Kyoto Univ Kyoto 121: 35–41Google Scholar
  20. Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid medium. Planta 126: 105–110CrossRefGoogle Scholar
  21. Kyozuka J, Yasuyushi H, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206: 408–413CrossRefGoogle Scholar
  22. Lee B, Murdoch K, Topping J, de Both MTJ, Wu QS, Karp A, Steele S, Symonds C, Kreis M, Jones MGK (1988) Isolation, culture and morphogenesis from wheat protoplasts, and study of expression of DNA constructs by direct gene transfer. Plant Cell Tissue Organ Cult 12: 223–226CrossRefGoogle Scholar
  23. Lee B, Murdoch K, Topping J, Kreis M, Jones MGK (1989) Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat. Plant Mol Biol 13: 21–29PubMedCrossRefGoogle Scholar
  24. Li ZY, Xia GM, Chen HM (1992a) Somatic embryogenesis and plant regeneration from protoplasts isolated from embryogenic cell suspensions of wheat (Triticum aestivum L.). Plant Cell Tissue Organ Cult 28: 79–85CrossRefGoogle Scholar
  25. Li ZY, Xia GM, Chen HM, Guo GQ (1992b) Plant regeneration from protoplasts derived from embryogenic suspension cultures of wheat (Triticum aestivum L.). J. Plant Physiol 139: 714–718CrossRefGoogle Scholar
  26. Lörz H, Baker B, Schell J (1985) Gene transfer to cereal cells mediated by protoplast transformation. Mol Gen Genet 199: 178–182CrossRefGoogle Scholar
  27. Maddock SE (1987) Suspension and protoplast culture of hexaploid wheat (Triticum aestivum L.). Plant Cell Rep 6: 23–26CrossRefGoogle Scholar
  28. Moran N, Ehrenstein G, Iwasa K, Bare C, Mischke C (1984) Ion channels in plasmalemma of wheat protoplasts. Science 226: 835–838PubMedCrossRefGoogle Scholar
  29. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  30. Potrykus I, Petruska J (1983) Approaches to cereal protoplast culture: morphogenic cultures in wheat (Triticum). In: Potrykus I et al. (ed) Proc 6th Int Protoplast Symp. Birkhäuser, Basel, pp 12–13Google Scholar
  31. Potrykus I, Harms CT, Lörz H (1976) Problems in culturing cereal protoplasts. In: Dudits D, Farkas GL, Maliga P (eds) Cell genetics in higher plants. Akademiai Kiado, Budapest, pp 129–140Google Scholar
  32. Qiao YM, Cattaneo M, Cocatelli F, Lupotto E (1992) Plant regeneration from long term suspension culture-derived protoplasts of hexaploid wheat (Triticum aestivum L.). Plant Cell Rep 11: 262–265Google Scholar
  33. Santakumari M, Berkowitz GA (1990) Correlation between the maintenance of photosynthesis and in situ protoplast volume at low water potentials in droughted wheat. Plant Physiol 92(3): 733–739Google Scholar
  34. Sethi M, Maeda E (1983) Studies on wheat protoplasts — a rapid and large-scale isolation method and cell wall regeneration in culture. Jpn J Crop Sci 52: 158–167CrossRefGoogle Scholar
  35. Shillito RD, Saul MW, Paszkowski J, Muller M, Potrykus I (1985) High efficiency direct gene transfer to plants. Bio/Technology 3: 1099–1103CrossRefGoogle Scholar
  36. Sun BL, Sun YG, Zhu Z, Li XH (1990) High frequency plant regeneration from protoplasts of wheat. Chin J Biotechnol 6(2): 125–129Google Scholar
  37. Vasil IK, Vasil V (1991) Advances in cereal protoplast research. Physiol Plant 82(1): AlGoogle Scholar
  38. Vasil IK, Vasil V, Redway F (1990) Plant regeneration from embryogenic calli, cell suspension cultures and protoplasts of Triticum aestivum L. (wheat). In: Nijkamp HJJ, van der Plas LHW, Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 33–37CrossRefGoogle Scholar
  39. Vasil V, Redway F, Vasil IK (1990) Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio/Technology 8: 429–434CrossRefGoogle Scholar
  40. Wang HB, Li XH, Sun BL, Fang R, Wang P, Chen J, Zhu Z, Zhang LM, Zhang W, Wei JK, Lan JS, Sun YG (1988) Plantlet regeneration from protoplast of wheat. Genet Manipulation Crops News 4(2): 11–16Google Scholar
  41. Wang HC, Li JS (1987) The difference in freezing tolerance between protoplast and vacuole of wheat leaves. Plant Biol 5: 221–228Google Scholar
  42. Wang WC, Nguyen TH (1990) A novel approach for efficient plant regeneration from long-term suspension culture of wheat. Plant Cell Rep 8: 639–642CrossRefGoogle Scholar
  43. Werr W, Lörz H (1986) Transient gene expression in a Gramineae cell line. Mol Gen Genet 202: 471–475CrossRefGoogle Scholar
  44. Yang YM, He DD, Scott KJ (1993) Plant regeneration from protoplasts of durum wheat (Triticum durum Desf. cv. D6962). Plant Cell Rep 12: 320–323CrossRefGoogle Scholar
  45. Zaghmout OMF, Trolinder NL (1993) Factors affecting transient gene expression in protoplasts isolated from very slowly growing embryogenic callus cultures of wheat. Theor Appl Genet 86: 721–730CrossRefGoogle Scholar
  46. Zhou H, Stiff CM, Konzak CF (1993) Stably transformed callus of wheat by electroporationinduced direct gene transfer. Plant Cell Rep 12: 612–616CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Y-F. Chang
    • 1
  • J. R. Wong
    • 2
  1. 1.CIBA-GEIGY Corporation, Agricultural Biotechnology UnitResearch Triangle ParkUSA
  2. 2.Genetic System DivisionBio-Rad LaboratoriesHerculesUSA

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