Abstract
The production of an extracellular polysaccharide capsule is a common feature of many bacteria (Whitfield and Valvano 1993). The capsule, which often constitutes the outermost layer of the cell, mediates the interaction between the bacterium and its immediate environment and plays a crucial role in the survival of bacteria in hostile environments. One such environment is the human host, where interactions between the capsule and the host’s immune system may be vital in deciding the outcome of an infection (Moxon and Kroll 1990). In the absence of specific antibody, a capsule offers protection against the nonspecific arm of the host’s immune system by conferring increased resistance to complement-mediated killing and complement-mediated opsonophagocytosis (Michalek et al. 1988; Moxon and Kroll 1990). A small set of capsular polysaccharides which resemble polysaccharide moieties present in host tissue are poorly immunogenic (Finne 1982; Lindahl et al. 1994). The Escherichia coli K1 and Neisseria meningitidis serogroup B capsules, both of which contain N-acetyl-neuraminic acid and the heparin-like E. coli K5 capsule, all elicit a poor antibody response in infected individuals (Jennings 1990) and confer some measure of resistance to the host’s adaptive humoral response. Aside from direct interactions with the host’s immune system, capsules may promote the formation of biofilms and the colonisation of a variety of ecological niches, including indwelling catheters, prostheses and the formation of alginate-rich biofilms in the lungs of cystic fibrosis patients (Roberts 1995). In such instances the polysaccharide may present a permeability barrier to antibiotics and hinder the effective eradication of the bacteria (Costerton et al. 1999).
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References
Armor PA, Whitfield C (1997) Molecular and functional analysis of genes required for the expression of group IB K antigens in Escherichia coli: characterisation of the his-region containing gene clusters for multiple cell surface polysaccharides. Mol Microbiol 26: 145–161
Bernheimer HP, Wermundsen IE (1969) Unstable binary capsulated transformants in pneumococcus. J Bacteriol 98: 1073–1079
Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997) The complete genome sequence of Escherichia coli K-12. Science 277: 1453–1462
Boyd EF, Hartl DL (1998) Chromosomal regions specific to pathogenic isolates of Escherichia coli have a phylogenetically clustered distribution. J Bacteriol 180: 1159–1165
Caimano MJ, Hardy GG, Yother J (1997) Capsule genetics in Streptococcus pneumoniae and a possible role for transposition in the generation of the type 3 locus. Microb Drug Resist 4: 11–23
Chaffin DO, Beres SB, Yim HY, Rubens CE (2000) The serotype of type la and III group B streptococci is determined by the polymerase gene within the polycistronic operon. J Bacteriol 182: 4466–4477
Cheetham BF, Katz ME (1995) A role for bacteriophages in the evolution and transfer of bacterial virulence determinants. Mol Microbiol 18: 201–208
Clarke BR, Pearce R, Roberts IS (1999) Genetic organisation of the Escherichia coli K10 capsule gene cluster: identification and characterisation of two conserved regions in group III capsule gene clusters encoding polysaccharide transport functions. J Bacteriol 181: 2279–2285
Claus H, Vogel U, Muhlenhoff M, Gerardy-Schahn R, Frosch M (1997) Molecular divergence of the sia locus in different serogroups of Neisseria meningitidis expressing polysialic acid capsules. Mol Gen Genet 257: 28–34
Claverys J-P, Prudhomme M, Mortier-Barriere I, Martin B (2000) Adaptation to the environment: Streptococcus pneumoniae, a paradigm for recombination-mediated genetic plasticity. Mol Microbiol 35: 251–259
Costerton JW, Stewart, PS Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284: 1318–1322
Dodgson C, Amor P, Whitfield, C (1996) Distribution of the rol gene encoding the regulator of lipopolysaccharide 0-chain length in Escherichia coli and its influence on the expression of group I capsular antigens. J Bacteriol 178: 1895–1902
Drummelsmith J, Whitfield C (1999) Gene products required for cell surface expression of the capsular form of the group 1 K antigen in Escherichia coli (O9a:K30). Mol Microbiol 31: 1321–1332
Drummelsmith J, Amor PA, Whitfield C (1997) Polymorphism, duplication and IS-1-mediated rearrangement in the his-rfb-gnd region of Escherichia coli strains with group IA capsular-K-antigens. J Bacteriol 179: 3232–3238
Edwards U, Muller A, Hammerschmidt S, Gerady-Schahn R, Frosch M (1994) Molecular analysis of the biosynthesis pathway of the alpha-2,8 polysialic acid capsule by Neisseria meningitidis serogroup B. Mol Microbiol 14: 141–149
Finne J (1982) Occurrence of unique polysialosyl carbohydrate units in glycoproteins of developing brain. J Biol Chem 257: 11966–11970
Frosch M, Müller A (1993) Phospholipid substitution of capsular polysaccharides and mechanisms of capsule formation in Neisseria meningitidis. Mol Microbiol 8: 483–493
Frosch M, Edwards U, Bousset K, Kauße B, Weisgerber C (1991) Evidence for a common molecular origin of the capsule gene loci in gram-negative bacteria expressing group II capsular polysaccharides. Mol Microbiol 5: 1251–1263
Garcia E, Arrecubieta C, Munoz R, Mollerach M, Lopez R (1997) A functional analysis of the Streptococcus pneumoniae genes involved in the synthesis of the type 1 and type 3 capsular polysaccharides. Microb Drug Resist 3: 73–88
Garcia E, Llull D, Munoz R, Mollerach M, Lopez R (2000) Current trends in capsular polysaccharide biosynthesis of Streptococcus pneumoniae. Res Microbiol 151: 429–435
Griffith F (1928) The significance of pneumococcal types. J Hyg 27: 113–159
Hammerschmidt S, Birkholz C, Zahringer U, Robertson BD, van Putten J, Ebeling O, Frosch M (1994) Contribution of genes from the capsule gene cluster complex (cps) to lipo-oligosaccharide biosynthesis and serum resistance in Neisseria meningitidis. Mol Microbiol 11: 885–896
Hammerschmidt S, Hilse R, van Putten JP, Gerardy-Schahn R, Unkmeir A, Frosch M (1996a) Modulation of cell surface sialic acid expression in Neisseria meningitidis via a transposable genetic element. EMBO J 15: 192–198
Hammerschmidt S, Muller A, Sillmann H, Muhlenoff M, Borrow R, Fox A, van Putten JP, Zollinger WD, Gerardy-Schahn R, Frosch M (1996b) Capsule phase variation in Neisseria meningitidis serogroup B by slipped strand mispairing in the polysialyl transferase gene (siaD): correlation with bacterial invasion and the outbreak of meningococcal disease. Mol Microbiol 20: 121l - 1220
Hardy GG, Caimano MJ, Yother J (2000) Capsule biosynthesis and basic metabolism in Streptococcus pneumoniae are linked through the cellular phosphoglucomutase. J Bacteriol 182: 1854–1863
Jann B, Jann K (1997) Capsules of Escherichia coli. In: Sussman M (ed) Escherichia coli mechanisms of virulence. Cambridge University Press, Cambridge, pp 113–143
Jann K, Jann B (1992) Capsules of Escherichia coli, expression and biological significance. Can J Microbiol 38: 705–710
Jennings HJ (1990) Capsular polysaccharides as vaccine candidates. Curr Top Microbiol Immunol 150: 97–127
Jones DM (1995) Epidemiology of meningococcal disease in Europe and the USA. In: Cartwright K (ed) Meningococcal disease. John Wiley and Sons, Chichester, pp 147–157
Kelly RF, MacLean LL, Perry MB, Whitfield C (1995) Structure of the 0-antigens of Klebsiella serotypes O2(2a, 2e), O2(2a, 2e, 2h) O2(2a, 2f, 2g), members of a family of related D-galactan 0-antigens in Klebsiella spp. J Endotoxin Res 2: 131–140
Klein NJ, Ison CA, Peakman M, Hammerschmidt S, Frosch M, Heyderman RS (1996) The influence of capsulation and lipo-oligosaccharide structure on neutrophil adhesion molecule expression and endothelial injury by Neisseria meningitidis. J Infect Dis 173: 172–179
Lavitola A, Bucci C, Salvatore P, Maresca G, Bruni CB, Alifano P (1999) lntracistronic transcription termination in polysialyltransferase gene (siaD) affects phase variation in Neisseria meningitidis. Mol Microbiol 33: 119–127
Lee CY (1995) Association of the staphylococcal type-1 capsule-encoding genes with a discrete genetic element. Gene 29: 115–119
Lindahl U, Lidholt K, Spillmann D, Kjellen L (1994) More to heparin than anticoagulation. Thrombin Res 75: 1–32
Michalek MT, Bremer EG, Mold C (1988) Effect of gangliosides on activation of the alternative pathway of human complement. J Immunol 140: 1581–1587
Morona JK, Morona R, Paton JC (1999) Analysis of the 5’ portion of the type 19A capsule locus identifies two classes of cpsC, cpsD and cpsE genes in Streptococcus pneumoniae. J Bacteriol 181: 3599–3605
Morona JK, Paton JC, Miller DC, Morona R. (2000) Tyrosine phosphorylation of CpsD negatively regulates capsular polysaccharide biosynthesis in Streptococcus pneumoniae. Mol Microbiol 35: 1431–1442
Moxon ER, Kroll JS (1990). The role of bacterial polysaccharide capsules as virulence factors. Curr Top Microbiol Immunol 150: 65–86
Munoz R, Garcia E, Lopez R (1998) Evidence for horizontal transfer from Streptococcus to Escherichia coli of the kfiD gene encoding the K5-specific UDP-glucose dehydrogenase. J Mol Evol 46: 324–326
Nelson K, Selander RK (1994) Intergeneric transfer and recombination of the 6-phosphogluconate dehydrogenase gene (gnd) in enteric bacteria. Proc Natl Acad Sci USA 91: 10227–10231
Ochman H, Bergthorsson U (1998) Rates and patterns of chromosome evolution in enteric bacteria. Curr Opin Microbiol 1: 580–583
Orskov I, Nyman K (1974) Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli. J Bacteriol 120: 43–51
Pearce R, Roberts IS (1995) Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of serA-linked capsule gene clusters. J Bacteriol 177: 3992–3997
Perna NT, Mayhew GF, Pósfal G, Elliott S, Donnenberg MS, Kaper JB, Blattner FR (1998) Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect Immun 66: 3810–3817
Petering H, Hammerschmidt S, Frosch M, van Putten J, Ison CA, Robertson BD (1996) Genes associated with meningococcal capsule complex are also found in Neisseria gonorrhoeae. J Bacteriol 178: 3342–3345
Picard B, Garcia JS, Gouriou S, Duriez P, Brahimi N, Bingen E, Elion J, Denamur E (1999) The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infect Immun 67: 546–553
Rahn A, Drummelsmith J, Whitfield C (1999) Conserved organization in the gene clusters for expression of Escherichia coli group 1 K antigens: relationship to the colanic acid biosynthesis locus and the genes from Klebsiella pneumoniae. J Bacteriol 181: 2307–2313
Roberts IS (1995) Bacterial polysaccharides in sickness and in health. Microbiol 141:2023–2031 Roberts IS (1996) The biochemistry and genetics of capsular polysaccharide production in bacteria. Ann Rev Microbiol 50: 285–315
Roberts IS, Mountford R, High N, Bitter-Suerman D, Jann K, Timmis KN, Boulnois GJ (1986) Molecular cloning and analysis of the genes for production of the K5, K7, K12, and K92 capsular polysaccharides of Escherichia coli. J Bacteriol 168: 1228–1233
Roberts IS, Mountford R, Hodge R, Jann KB, Boulnois GJ (1988) Common organization of gene clusters for the production of different capsular polysaccharides (K antigens) in Escherichia coli. J Bacteriol 170: 1305–1310
Russo TA, Wenderoth S, Carlino UB, Merrick JM, Lessie AJ (1998) Isolation, genomic organization, and analysis of the group III capsular polysaccharide genes kpsD, kpsM, kpsT, and kpsE from an extraintestinal isolate of Escherichia coli (CP9, O4/K54/H5). J Bacteriol 180: 338–349
Sau S, Bhasin N, Wann ER, Lee JC, Foster TJ, Lee CY (1997) The Staphylococcus aureus allelic genetic loci for serotype 5 and 8 capsule expression contain type-specific genes flanked by common genes. Microbiol 143: 2395–2405
Steenbergen S, Vimr E (1990) Mechanism of polysialic acid chain elongation in Escherichia coli K1. Mol Microbiol 4: 603–611
Svenson D, Bukanov NO, Berg DE, Welch RA (1996) Two pathogenicity islands in uropathogenic Escherichia coli J 96:cosmid cloning and sample sequencing. Infect Immun 64: 3736–3743
Swartley JS, Martin AA, Edupuganti S, Liu L-J, Cieslak P, Perkins B, Wenger JD, Stephens DS (1997) Capsule switching of Neisseria meningitidis. Proc Natl Acad Sci USA 94: 271–276
Swartley JS, Liu L-J, Miller YK, Martin LE, Edupuganti S, Stephens DS (1998) Characterisation of the gene cassette required for biosynthesis of the (71–6)-linked N-acetyl-D-Mannosamine-l-phosphate capsule of serogroup A Neisseria meningitides. J Bacteriol 180: 1533–1539
Timmerman KP, Tu CPD (1985) Complete sequence of IS3. Nucleic Acids Res 13: 2127–2139
Tinsley CR, Nassif X (1996) Analysis of the genetic differences between Neisseria meningitidis and Neisseria gonorrhoeae: two closely related bacteria expressing two different pathogenicities. Proc Natl Acad Sci USA 93: 11109–11114
Wacharotayankun R, Arakawa Y, Ohta M, Tanaka K, Akashi T, Mori M, Kato N (1993) Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ. Infect Immun 61: 3164–3174
Whitfield C, Roberts IS (1999) Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol Microbiol 31: 1307–1319
Whitfield C, Valvano MA (1993) Biosynthesis and expression of cell surface polysaccharides in gram-negative bacteria. Adv Micro Physiol 35: 135–246
Ziebuhr W, Krimmer V, Rachid S, Lossner I, Gotz F, Hacker J (1999) A novel mechanism of phase variation of virulence of Staphylococcus epidermidis:evidence for control of the polysaccharide intercellular adhesin synthesis by alternating insertion and excision of the insertion sequence element IS256. Mol Microbiol 32: 345–356
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Barrett, B., Ebah, L., Roberts, I.S. (2002). Genomic Structure of Capsular Determinants. In: Hacker, J., Kaper, J.B. (eds) Pathogenicity Islands and the Evolution of Pathogenic Microbes. Current Topics in Microbiology and Immunology, vol 264/1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09217-0_8
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DOI: https://doi.org/10.1007/978-3-662-09217-0_8
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