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Design, Quality Control and Normalization of Biosensor Chips

  • Claudia Preininger
  • Ursula Sauer
Chapter
Part of the Springer Series on Chemical Sensors and Biosensors book series (SSSENSORS, volume 1)

Abstract

With the completion of the human genome project biochip technologies have boosted and revolutionized automated genomic and proteomic analysis (www.microarrays.org; www.gene-chips.com) [1–7]. Based on conventional biomolecular techniques such as Southern and Northern blotting, sample preparation and assay was miniaturized by micromachining and microbiochemistry implemented efficiently by automated processes. To use biochip technologies for high throughput applications the system was adapted for high levels of parallelization. The potential of biochips lies in the parallel analysis of a huge number of probes, measured at once instead of one probe after the other. Such a technique speeds up biomolecular analysis tremendously. DNA chips have been widely used for gene expression, functional analysis, gene mapping and genotyping. Measuring RNA levels, however, might not give a complete or accurate description of a biological system. Because proteins mediate nearly all cellular activities, biochips have also been applied at the protein level (“proteomics”) [8, 9].

Keywords

Fluorescence Resonance Energy Transfer Oligonucleotide Array Chip Surface Chip Analysis Glass Chip 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Claudia Preininger
  • Ursula Sauer

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