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TIRF Array Biosensor for Environmental Monitoring

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Optical Sensors

Part of the book series: Springer Series on Chemical Sensors and Biosensors ((SSSENSORS,volume 1))

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Abstract

The field of biosensors has been the subject of an increasing number of books and literature reviews [1–5]. Biosensors consist of two major components: the molecular recognition element and a signal transduction mechanism. The molecular recognition element in biosensor technology takes the form of either a biological molecule (antibodies, enzymes or nucleic acids), a biological system (membranes, tissues or whole cells) or a biomimetic (a species which consists of an active site comparable to that of a naturally produced biomolecule). The increase in sensitivity and specificity, in comparison to chemical sensors, is a direct result of utilizing a biomolecule as the recognition element. The choice of transduction method largely depends on the intrinsic properties of the biomolecule, such as the co-factors found within the structure, and the recognition event measured. Typical transduction techniques used in array biosensor formats include optical and electrochemical; however, mass-mechanical, thermal and acoustic are also possibilities.

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Sapsford, K.E., Ligler, F.S. (2004). TIRF Array Biosensor for Environmental Monitoring. In: Optical Sensors. Springer Series on Chemical Sensors and Biosensors, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09111-1_14

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  • DOI: https://doi.org/10.1007/978-3-662-09111-1_14

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