Skip to main content
SpringerLink
Log in
Book cover

Monoklonale Antikörper pp 75–86Cite as

Kontrolle von Zellkulturen

  • Chapter

  • 70 Accesses

Part of the book series: Hochschultext ((HST))

Zusammenfassung

Zwei Fluoreszenzfarbstoffe werden kombiniert eingesetzt, um lebendige und tote Zellen unterschiedlich anzufärben. Fluoreszein-Diazetat (FDA) wird als nicht fluoreszierende Vorstufe nur von lebendigen Zellen aufgenommen, hydrolytisch gespalten und dadurch in eine fluoreszierende Verbindung (Fluoreszein) umgewandelt. Ethidiumbromid (EtBr) bleibt von lebendigen Zellen ausgeschlossen und dringt nur in tote Zellen ein, wo es den Kern rot darstellt.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   54.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Weiterführende Literatur

  • Göhde W, Dittrich W (1978) Impulsfluorometrie — ein neuartiges Durchflußverfahren zur ultraschnellen Mengenbestimmung von Zeilinhaltsstoffen. Acta histochem Suppl X: 429–437.

    Google Scholar 

  • NN Cell viability testing using Erythrosin B staining. Protocol Nr. G 19 der Firma Biophysika Systems.

    Google Scholar 

Literatur

  • Greene LM, Reade JL, Ware CF. (1984) Rapid colorimetric assay for cell viability: application to the Quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Meth 70: 257–268.

    Article  Google Scholar 

  • Herrmann EC, Gablikis J, Engle C, Perlman PL. (1960) Agar diffusion method for detection and bioassay of antiviral antibiotics. Proc Soc Exp Biol Med 103: 625–628.

    PubMed  CAS  Google Scholar 

  • Mosmann T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J Immunol Meth 65: 5563.

    Article  Google Scholar 

  • Baumgarten H (1985) A simple microplate assay for the determination of cellular protein. J Immunol Meth, im Druck.

    Google Scholar 

  • Sedmak JJ, Grossberg SE. (1977) A rapid, sensitive and versatile assay for protein using Coomassie brilliant Blue G-250. Anal Biochem 79: 544–552.

    Article  PubMed  CAS  Google Scholar 

  • Bradford M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. J. H. Peters
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Baumgarten
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Peters, J.H., Baumgarten, H. (1985). Kontrolle von Zellkulturen. In: Monoklonale Antikörper. Hochschultext. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08843-2_6

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-08843-2_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-15808-0

  • Online ISBN: 978-3-662-08843-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation