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Monoklonale Antikörper pp 259–283Cite as

Reinigung von monoklonalen Antikörpern und Herstellung von Antikörper-Fragmenten

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Zusammenfassung

Der Verwendungszweck eines monoklonalen Antikörpers (MAK) bestimmt wesentlich den erforderlichen Reinheitsgrad einer Antikörperpräparation. Wird beispielsweise in der indirekten Immunfluoreszenz oder -zytochemie ein ausreichend spezifisches anti-MAK Nachweiskonjugat eingesetzt, erübrigt sich in den meisten Fällen eine weitere Aufreinigung des konzentrierten Kulturüberstandes oder von Aszites. Sollen MAK hingegen direkt markiert werden (s. Kap. 9 ff.), muß eine Kontamination mit Fremdproteinen (wie Insulin oder Transferrin) aus dem Mausaszites oder dem Kulturmedium ausgeschlossen werden, da diese über eine Bindung an ihre spezifischen membranständigen Rezeptoren eine Bindung des MAK an diese Zellen vortäuschen könnten. Aus ähnlichen Gründen verbietet sich der Einsatz nur ungenügend gereinigten Materials in biologischen Testsystemen, in denen Kontaminanten Effekte bewirken können, die fälschlicherweise den MAK zugeschrieben würden. Ein Antikörper der gleichen Immunglobulinsubklasse, der nicht mit dem fraglichen Antigen reagiert, aber aus derselben Fusion stammt und unter gleichen Bedingungen hergestellt wurde, kann in derartigen Fällen als Kontrollantikörper dienen. Wiederum besonderen Kriterien müssen MAK-Präparationen genügen, die zu therapeutischen Zwecken in-vivo eingesetzt werden sollen (s. Kap. 1.3).

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Authors
  1. M. Oppermann
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  2. R. Würzner
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  3. M. Schulze
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  4. H. Baumgarten
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  5. R. Zierz
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Editor information

Editors and Affiliations

  1. Abteilung für Immunologie, Zentrum für Hygiene und Humangenetik, Georg-August-Universität, Kreuzbergring 57, D-3400, Göttingen, Deutschland

    Johann Hinrich Peters

  2. Diagnostika Forschung, Boehringer Mannheim GmbH, Bahnhofstraße 9-15, D-8132, Tutzing, Deutschland

    Horst Baumgarten

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© 1990 Springer-Verlag Berlin Heidelberg

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Oppermann, M., Würzner, R., Schulze, M., Baumgarten, H., Zierz, R. (1990). Reinigung von monoklonalen Antikörpern und Herstellung von Antikörper-Fragmenten. In: Peters, J.H., Baumgarten, H. (eds) Monoklonale Antikörper. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08842-5_8

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  • DOI: https://doi.org/10.1007/978-3-662-08842-5_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-50844-1

  • Online ISBN: 978-3-662-08842-5

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