Abstract
In the present study gas chromatography-mass spectrometry (GC-MS) methods were applied for investigation of worm compost (via Eisenia fetida culture). Sewage sludge (SS) and cattle manure (CM) were used as raw matter for compost preparation. Total percentage of carbon increased from 13% (for SS vermicompost) up to 50% (CM vermicompost), while the ratio of C:N from 22 – 42 up to 22 for SS and CM, respectively, has been shown. The contents of total nitrogen (N = 1−2%), potassium (K20 = 1.1−1.4%) and phosphorus (P205 = 0.6−1.5%) have been measured. The concentrations of heavy metals such as Cd, Cu, Cr, Ni, Pb, Zn in organic matter after digestion by earthworms decreased by two to six times. The greatest reduction was observed for Cr and Zn concentrations and the smallest for Ni. Lipid biomarker analysis revealed altogether 21 genera of microorganisms in vermicomposts community representing a number of trophical groups. The abundance of microorganisms from genera Acetobacter, Sphingobacterium, Aeromonas, Vibrio and Streptomyces increased remarkably after SS vermicomposting procedure. Quantitative comparison of microbial biodiversity showed, that three genera dominated in CM vermicompost: Bdellovibrio (and/or Spirillum) usually inhabitanting in wastewater (17%); Bacteroides (15%) and Clostridium (17%), which are common for rumen. The four following genera dominated in cenoses from SS composts: Acetobacter (20%), Pseudomonas (11%), Wolinella (12%) and Bacteroides (10%), making totally 61%. Supposedly, prevalence of genera Acetobacter and Wolinella may improve nitrogen metabolism in compost matter. The SS community had two dominating genera: Pseudomonas (27%) and Bacteroides (37%). Acquired data demonstrates prevalence of microorganisms with high hydrolyzing activity. They are spore forming (Bacillus), mycelial (Streptomyces, Nocardia, representatives of the Maduromycetes group) and cellulolytic (Cytophaga) microorganisms. Their products serve as substrates for fermenting organisms (Bacteroides, Clostridium, Vibrio, Wolinella). Later, members of Acetobacter, Pseudomonas, Sphingobacterium, Sphingomonas use simple organic material formed after hydrolysis and fermentation in aerobic conditions.
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References
Brondz J, Olsen J (1986) Microbial chemotaxonomy, chromatography, electrophoresis and relevant profiling techniques. J Chromatogr Biomed Appl 379: 367–411
Brown GG (1995) How do earthworms affect microbfloral and faunal community diversity? Plant Soil 170: 209–231
Carpenter-Boggs L, Kennedy AC, Reganold JP (1998) Use of phospholipid acids carbon source utilization patterns to track microbial community succession in developing compost. Appl Environ Microbiol 64: 4062–4064
Dobrovol’skaya TG, Chernov IYu, Zvyagintsev DG (1997) Characterizing the structure of bacterial communities Microbiologia 66: 408–414
Garland JL, Mills AL (1994) A community-level physiological approach for studying microbial communities. In: Ritz K, Dighton J and Giller KE (eds) Beyond the biomass: compositional and functional analysis of soil microbial communities. Wileys, New York, pp 77–83
Goodfellow M, Minnikin DE (eds) (1985) Chemical methods in bacterial systematics. Academic Press, London
Guo Yongcan (1995) Soil heavy metal pollution and earthworm isozyme. Chinese J Appl Ecol 6: 316–322
Guo Yongcan, Wang Zhenzhong, Zhang Youmei, Mo Xiaoyang (1998) Bioconcentration effects of heavy metal pollution in soil on the mucosa, epithelia cell ultrastructure injuring of the eartworm’s gastrointtestinal tract. Bull Env Contam Toxicol 60: 280–284
Insam H, Amor K, Renner M, Crepaz C (1996) Changes in functional abilities of the microbial community during composting of manure. Microb Ecol 31: 77–87
Madsen EL, Alexander M (1982) Transport of Rhizobium and Pseuclomonas though soil. Soil Sci Soc Am J 46: 557–560
McNabb A, Shuttleworth R, Behme R (1997) Fatty acid characterization of rapidly growing pathogenic aerobic actinomycctes as a means of identification. J Clin Microbiol 35: 1361–1368
Marialigeti K (1979) On the community-structure of the gut-microbiota of Eisenia lucens (Annelida, Oligochaeta). Pedobiologia 19: 231–220
Nikitin DI, Kunc F. (1988) Structure of microbial soil associations and some mechanisms of their autoregulation. In Vancura V, Kunc F (eds) Soil microbial associations: control of structures and functions. Academia, Praga
Osipov GA, Turova ES (1997) Studying species composition of microbial communities with the use of gas chromatography-mass spectrometry. Microbial community of kaolin. FEMS Microb Rev 20: 437–446.
Paul EA, Clark FE (1989) Soil microbiology and biochemistry.Academic Press, San Diego
Pedersen JC, Hendriksen NB (1993) Effect of passage though the intestinal tract of detritivore earthworms ( Lumbricus spp.) on the number of selected Gram-negative and total bacteria. Biol Fertil Soils 16: 227–232
Pennanen T, Frostegard A, Fritze H, Baath E (1996) Phospholipid fatty-acid composition and heavy metal tolerance of soil microbial communities along 2 heavy metal-polluted gradients in coniferous forests. Appl Environ Microbiol 62: 420–428
Steward CC, Nold SC, Ringelberg DB, White DC, Lovell CR (1996) Microbial biomass and community structures in the burroms of bromophenol producing and nonproducing marine worms and surrounding sediments 133: 149–165
Toyota K, Kimura M (2000) Microbial community indigenous to the earthworm Eisenia foetida. Biol Fertil Soil 31: 187–190
Wang Zhehzhong (1994) Effect of heavy metals in soil on earthworms (Opisthora). Acta Sci Cirum 2: 237–243
Weyant RS, Moss CW, Weaver RE, Hollis DG, Jordan JG, Cook EC, Daneshvar MJ (1996) Identification of unusual pathogenic Gram-negative aerobic and facultatively anaerobic bacteria. Second edition. Williams & Wilkins, Baltimore-Philadelphia
White DC, Davis WM, Nickels JS, King JD, Robbie RJ (1979) Determination of the sedimentary microbial biomass by extractable lipid phosphate. Ecology 40: 51–62
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© 2002 Springer-Verlag Berlin Heidelberg
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Verkhovtseva, N.V. et al. (2002). Comparative Investigation of Vermicompost Microbial Communities. In: Insam, H., Riddech, N., Klammer, S. (eds) Microbiology of Composting. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08724-4_8
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DOI: https://doi.org/10.1007/978-3-662-08724-4_8
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