Genetic Engineering of Bacterial and Eukaryotic Ribosomal Proteins for Investigation on Elongation Arrest of Nascent Polypeptides and Cell Differentiation

  • Fotini Leontiadou
  • Christina Matragkou
  • Filippos Kottakis
  • Dimitrios L. Kalpaxis
  • Ioannis S. Vizirianakis
  • Sofia Kouidou
  • Asterios S. Tsiftsoglou
  • Theodora Choli-Papadopoulou
Part of the Principles and Practice book series (PRINCIPLES)

Abstract

This paper describes our efforts to investigate the role of specific bacterial and eukaryotic ribosomal proteins in crucial cell functions such as elongation arrest of the nascent polypeptides and cell differentiation. These objectives have been approached by: (1) engineering the L4 bacterial ribosomal protein, which has been shown by crystallographic data to be a candidate molecule for controlling the nascent polypeptides before their emerge from the ribosome, to elucidate specific amino-acids functions in highly conserved regions; and (2) isolating specific eukaryotic genes, like S5 and L35a, that encode ribosomal proteins to study their possible involvement in hematopoietic cell differentiation and apoptosis.

Keywords

Attenuation Leukemia Catalysis Electrophoresis Oncol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Fotini Leontiadou
  • Christina Matragkou
  • Filippos Kottakis
  • Dimitrios L. Kalpaxis
  • Ioannis S. Vizirianakis
  • Sofia Kouidou
  • Asterios S. Tsiftsoglou
  • Theodora Choli-Papadopoulou

There are no affiliations available

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