Phyllanthus Species: In Vitro Culture and the Production of Secondary Metabolites

  • D. W. Unander
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 37)


Phyllanthus is a large and complex genus in the Euphorbiaceae, currently thought to contain between 550 and 750 species in 11 subgenera (Webster 1970; Holm-Nielson 1979). While most commonly found in the tropics, species occur from tropical to mildly temperate zones on all continents except Europe and Antarctica. Some weedy species have become dispersed throughout most of the tropics, but many others are fairly restricted in known range (e.g., Webster 1956, 1957, 1958). Two fruit-bearing trees, P. acidus L. and P. emblica L., have been minor items of commerce for centuries. Some species have widely dispersed records of medicinal use, but generally the plants so used have not been cultivated (Unander and Blumberg 1991).


Root Culture Callus Culture Ellagic Acid Callus Growth Arnold Arbor 
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  1. Al-Kindi (ca. 850) The medical formulary or Aqrabadhin of Al-Kindi. Translated with a study of its Materia Medica by Martin Levey, 1966. University of Wisconsin Press, Madison, 235 ppGoogle Scholar
  2. Berk L, De Man RA, Schalm SW, Labadie RP, Heijtink RA (1991) Beneficial effects of Phyllanthus amarus for chronic hepatitis B, not confirmed. J Hepatol 12: 405–406PubMedCrossRefGoogle Scholar
  3. Dhir H, Roy AK, Sharma A (1993) Relative efficiency of Phyllanthus emblica fruit extract and ascorbic acid in modifying lead and aluminum-induced sister chromatid exchanges in mouse bone marrow. Environ Mol Mutagen 21: 229–236PubMedCrossRefGoogle Scholar
  4. Doshi JC, Vaidya AB, Antarkar DS, Deolalikar R, Antani DH (1994) A two-stage clinical trial of Phyllanthus amarus in hepatitis B carriers; failure to eradicate the surface antigen. Indian J Gastroenterol 13: 7–8PubMedGoogle Scholar
  5. Everett TH (ed) (1981) New York botanical garden illustrated encyclopedia of horticulture, vol 8. New York Botanical Garden, New York, pp 2616–2617Google Scholar
  6. Foo LY (1993a) Amarulone, a novel cyclic hydrolysable tannin from Phyllanthus amarus. Nat Prod Lett 3: 45–52CrossRefGoogle Scholar
  7. Foo LY (1993b) Amariin, a di-dehydrohexahydroxydiphenoyl hydrolysable tannin from Phyllanthus amarus. Phytochemistry 33: 487–491CrossRefGoogle Scholar
  8. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root callus. Exp Cell Res 50: 151–158PubMedCrossRefGoogle Scholar
  9. Gorski F, Correa CR, Filho VC, Yunes RA, Calixto JB (1993) Potent antinociceptive activity of a hydroalcoholic extract of Phyllanthus corcovadensis. J Pharm Pharmacol 45: 1046–1049PubMedCrossRefGoogle Scholar
  10. Haïcour R (1974) Comparaison chez Phyllanthus urinaria L. de l’activité antibactérienne des décoctions de diverses portions de la plante et de cultures de tissus qui en proviennent. CR Séances Acad Sci Paris Sér D 278: 3323–3325Google Scholar
  11. Ha:icour R (1975) Premiers éléments d’identification biochimique des principes antibactériens d’extraits du Phyllanthus urinario L. CR Séances Acad Sci Paris Sér D 280: 1789–1792Google Scholar
  12. Holm-Nielsen LB (1979) Comments on the distribution and evolution of the genus Phyllanthus (Euphorbiaceae). In: Larsen K, Holm-Nielsen LB (eds) Tropical botany. Academic Press, New York, pp 277–290Google Scholar
  13. Huer Y-H, Zeng W, Stoner GD, Nemeth GA, Hilton B (1992) Synthesis of ellagic acid o-alkyl derivatives and isolation of ellagic acid as a tetrahexanoyl derivative from Fragaria ananassa. J Nat Prod 55: 1402–1407CrossRefGoogle Scholar
  14. Ishimaru K, Yoshimatsu K, Yamakawa T, Kamada H, Shimomura K (1992) Phenolic constituents in tissue cultures of Phyllanthus niruri. Phytochemistry 31: 2015–2018CrossRefGoogle Scholar
  15. Kangsu Medical Institute (1975) Encyclopedia of Chinese medicine, 3 vols. Shanghai Publisher of Science and Technology. Citation No 3104 ( Chinese). Translated by Marilyn Wong, Fox Chase Cancer Center, PhiladelphiaGoogle Scholar
  16. Khanna P, Nag TN (1973) Isolation, identification and screening of phyllemblin from Emblica officinalis Gaertn. tissue culture. Indian J Pharm 35: 23–25Google Scholar
  17. Khanna P, Staba EJ (1968) Antimicrobials from plant tissue cultures. Lloydia 31: 180–189Google Scholar
  18. Leelarasamee A, Trakulsomboon S, Maunwongyathi P, Somanabandhu A, Pidetcha P, Matrakool B, Lebnak T, Ridthimat W, Chandanayingyong D (1990) Failure of Phyllanthus amarus to eradicate hepatitis B surface antigen from symptomless carriers. Lancet 1: 1600–1601CrossRefGoogle Scholar
  19. Linneaus C (1738) Hortus Cliffortianus. Amstel, p 438Google Scholar
  20. Mehrotra R, Rawat S, Kulshreshtha DK, Goyal P, Patnaik GK, Dhawan BN (1991) In vitro effect of Phyllanthus amarus on hepatitis B virus. Indian J Med Res (A) 93: 71–73Google Scholar
  21. Morton JF (1960) The emblic (Phyllanthus emblica L.). Econ Bot 14: 119–128CrossRefGoogle Scholar
  22. Munshi A, Mehrotra R, Panda SK (1993a) Evaluation of Phyllanthus amarus and Phyllanthus maderaspatensis as agents for postexposure prophylaxis in neonatal duck hepatitis B virus infection. J Med Virol 40: 53–58PubMedCrossRefGoogle Scholar
  23. Munshi A, Mehrotra R, Ramesh R, Panda SK (1993b) Evaluation of antihepadnavirus activity of Phyllanthus amarus and Phyllanthus maderaspatensis in duck hepatitis B virus carrier Pekin ducks. J Med Virol 41: 275–281PubMedCrossRefGoogle Scholar
  24. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  25. Niu J, Wang Y, Qiao M, Gowans E, Edwards P, Thyagarajan SP, Gust I, Locarnini S (190) Effect of Phyllanthus amarus on duck hepatitis B virus replication in vivo. J Med Virol 32: 212–218Google Scholar
  26. Ogata T, Higuchi H, Mochida S, Matsumoto H, Kato A, Endo T, Kaji A, Kaji H (1962) HIV-1 reverse transcriptase inhibitor from Phyllanthus niruri. AIDS Res Human Retrovirus 8: 1937–1944CrossRefGoogle Scholar
  27. Paskind MP, Weinberg RA, Baltimore D (1975) Dependence of Moloney murine leukemia virus production on cell growth. Virology 67: 242–248PubMedCrossRefGoogle Scholar
  28. Pettit GR, Schaufelberger DE (1988) Isolation and structure of the cytostatic lignan glycoside phyllanthostatin A. J Nat Prod 51: 1104–1112PubMedCrossRefGoogle Scholar
  29. Pettit GR, Cragg GM, Niven ML, Nassimbeni LR (1983) Structure of the principal antineoplastic glycosides of Phyllanthus acuminatus Vahl. Can J Chem 61: 2630–2632CrossRefGoogle Scholar
  30. Pettit GR, Cragg GM, Suffness MI, Gust D, Boettner FE, Williams M, Saenz-Renauld HA, Brown P, Schmidt JM, Ellis PD (1984) Antineoplastic agents. 104. Isolation and structure of the Phyllanthus acuminatus Vahl ( Euphorbiaceae) glycosides. J Org Chem 49: 4258–4266Google Scholar
  31. Pettit GR, Schaufelberger DE, Nieman RA, Dufresne C, Saenz-Renauld JA (1990) Antineoplastic agents 177. Isolation and structure of phyllanthostatin 6. J Nat Prod 53: 1406–1413PubMedCrossRefGoogle Scholar
  32. Rizk AFM (1987) The chemical constituents and economic plants of the Euphorbiaceae. Bot J Linn Soc 94: 293–326CrossRefGoogle Scholar
  33. Rossi M, Erlebacker J, Zacharias DE, Carrell HL, Tannucci B (1991) The crystal and molecular structure of ellagic acid dihydrate: a dietary anti-cancer agent. Carcinogenesis 12: 2227–2232PubMedCrossRefGoogle Scholar
  34. Santos ARS, Filho VC, Niero R, Viana AM, Moreno FN, Campos MM, Yunes RA, Calixto JB (1994) Analgesic effects of callus culture extracts from selected species of Phyllanthus in mice. J Pharm Pharmacol 46: 755–759PubMedCrossRefGoogle Scholar
  35. Santos ARS, Filho VC, Yunes RA, Calixto JB (1995) Further studies on the antinociceptive action of the hydroalcoholic extracts from plants of the genus Phyllanthus. J Pharma Pharmacol 47: 66–71CrossRefGoogle Scholar
  36. Sengupta P, Mukhopadhyay J (1966) Terpenoids and related compounds—VII: Triterpenoids of Phyllanthus acidus Skeels. Phytochemistry 5: 531–534CrossRefGoogle Scholar
  37. Shimizu M, Horie S, Terashima S, Ueno H, Hayashi T, Arisawa M, Suzuki S, Yoshizaki M, Morita N (1989) Studies on aldose reductase inhibitors from natural products. II. Active components of a Paraguayan crude drug, “Parai-parai mi”, Phyllanthus niruri. Chem Pharm Bull 37: 2531–2532PubMedCrossRefGoogle Scholar
  38. SomanabandhuA, Nitayangkura S, Mahidol C, Ruchirawat S, Likhitwitayawuid K, Shieh H-L, Chai H, Pezzuto JM, Cordell GA (1993) ’ H- and ’ 3C-NMR assignments of phyllanthin and hypophyllanthin: lignans that enhance cytotoxic responses with cultured multidrug-resistant cells. J Nat Prod 56: 233–239Google Scholar
  39. Tempesta MS, Corley DG, Beutler JA, Metral CJ, Yunes RA, Giacomozzi CA, Calixto JB (1988) Phyllanthimide, a new alkaloid from Phyllanthus sellowianus. J Nat Prod 51: 617–618PubMedCrossRefGoogle Scholar
  40. Thyagarajan SP, Subramanian S, Thirunalasundari T, Venkateswaran PS, Blumberg BS (1988) Preliminary study: the effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet 1988-II: 764–766Google Scholar
  41. Ueno H, Horie S, Nishi Y, Shogawa H, Kawasaki M, Suzuki S, Hayashi T, Arisawa M, Shimizu M, Yoshizaki M, Morita N, Berganza LH, Ferro E, Basualdo I (1988) Chemical and pharmaceutical studies on medicinal plants in Paraguay. Geraniin, an angiotensin-converting enzyme inhibitor from “paraparai mi”, Phyllanthus niruri. J Nat Prod 51: 357–359PubMedCrossRefGoogle Scholar
  42. Unander DW (1991) Callus induction in Phyllanthus spp. and inhibition of viral enzymes by callus extracts. Plant Cell Rep 10: 461–466CrossRefGoogle Scholar
  43. Unander DW, Blumberg BS (1991) In vitro activity of Phyllanthus (Euphorbiaceae) species against the DNA polymerase of hepatitis viruses: effects of growing environment and inter-and intra-specific differences. Econ Bot 45: 225–242CrossRefGoogle Scholar
  44. Unander DW, Webster GL, Blumberg BS (1990) Records of usage or assays in Phyllanthus (Euphorbiaceae). I. Subgenera Isocladus, Kirganelia, Cicca and Emblica. J Ethnopharmacol 30: 233–264PubMedCrossRefGoogle Scholar
  45. Unander DW, Webster GL, Blumberg BS (1991) Uses and bioassays in Phyllanthus (Euphorbiaceae): a compilation. II. The subgenus Phyllanthus. J Ethnopharmacol 34: 97–133PubMedCrossRefGoogle Scholar
  46. Unander DW, Webster GL, Blumberg BS (1992) Usage and bioassays in Phyllanthus (Euphorbiaceae): a compilation. III. The subgenera Conami, Gomphidium, Phyllanthodendron, Eriococcus, Xylophylla and Botryanthus and a complete list of the species cited in the three-part series. J Ethnopharmacol 36: 103–112PubMedCrossRefGoogle Scholar
  47. Unander DW, Bryan HH, Lance CJ, McMillan RT Jr (1993) Cultivation of Phyllanthus amarus and evaluation of variables potentially affecting yield and activity against viral DNA polymerase. Econ Bot 47: 79–88CrossRefGoogle Scholar
  48. Unander DW, Webster GL, Blumberg BS (1995a) Uses and bioassays in Phyllanthus ( Euphorbiaceae ): IV. Clustering of uses and effects within subgenera. J Ethnopharmacol 45: 1–18Google Scholar
  49. Unander DW, Bryan HH, McMillan RT Jr, Lance CJ (19956) Evaluation of variables affecting seed germination and plant stand of Phyllanthus amarus. Econ Bot 49: 49–55Google Scholar
  50. Venkateswaran PS, Millman I, Blumberg BS (1987) Effects of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: in vivo and in vitro studies. Proc Natl Acad Sci USA 84: 274–278PubMedCrossRefGoogle Scholar
  51. Webster GL (1956)A monographic study of the West Indian species of Phyllanthus. J Arnold Arbor Hary Univ 37: 91–122; 217–268; 340–359Google Scholar
  52. Webster GL (1957) A monographic study of the West Indian species of Phyllanthus (continued). J Arnold Arbor Hary Univ 38: 51–80; 170–198; 295–373Google Scholar
  53. Webster GL (1958) A monographic study of the West Indian species of Phyllanthus (continued). J Arnold Arbor Hary Univ 39: 49–100; 111–212Google Scholar
  54. Webster GL (1970) A revision of Phyllanthus (Euphorbiaceae) in the continental United States. Brittonia 22: 44–76CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • D. W. Unander
    • 1
  1. 1.Department of BiologyEastern CollegeSt. DavidsUSA

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