Agave amaniensis Trel & Nowell: In Vitro Culture and the Production of Phytosteroids

  • G. Indrayanto
  • W. Utami
  • A. Syahrani
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 37)


Agave amaniensis Trel & Nowell (family Agavaceae) was first found (under the name Agave lespinassei Trel) at the East African Agriculture Research Station, Amani, Tanganyika, in 1929. Its origin was unknown (Nowell 1933; Dahlgren et al. 1985). It is a perennial herb; habit erect, acaulescent, finally suckering rather freely. Leaves ensiform, rigid, leathery, (Fig. lA) ascending except for the short basal ones, which turn down; markedly glaucous on both sides, ground color deep, dull yellow-green, length 1.4–2 m, width at base 16 cm, at waist 10 cm, and at blade 15 cm. Flower and fruit are unknown. Propagation so far has depended on the transplanting of suckers, which are produced after 2 years of growth (Nowell 1933; Jacobsen 1978).


Callus Culture Growth Index Plant Cell Tissue Organ Cult Sapogenin Steroid Glutamate Dehydrogenase Activity 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. Indrayanto
  • W. Utami
  • A. Syahrani
    • 1
  1. 1.Laboratory of Pharmaceutical Biotechnology, Faculty of PharmacyAirlangga UniversitySurabayaIndonesia

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