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Trigonella Species: In Vitro Culture and Production of Secondary Metabolites

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Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 51))

Abstract

The Leguminosae (syn. Fabaceae) family is one of the three largest families of flowering plants. There is still no general agreement regarding the number of genera and species. Estimates vary between 590–690 genera and 12,000–17,000 species (Heywood 1971). The family is divided into three subfamilies: Caesalpinioïdeae, Mimosoïdeae and Papilionoïdeae (Brummitt 1992).

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References

  • Allen ON, Allen EK (1981) The Leguminosae. a source book of characteristics, uses and nodulations. Macmillan, Madison, pp 666–667

    Google Scholar 

  • Antony A, Gopinathan KP, Vaidyanathan CS (1975) Biosynthesis of trigonelline in root cultures of fenugreek (Trigonella foenum-graecum L.). Indian J Exp Biol 13:39–41

    CAS  Google Scholar 

  • Bräutigam M, Franz G (1985) Versuche zur Gewebekultur von schleimbildenden pflanzlichen Geweben. Sci Pharm 53:237–246

    Google Scholar 

  • Brain KR, Lockwood GB (1976) Hormonal control of steroid levels in tissue cultures from Trigonella foenumgraecum. Phytochemistry 15:1651–1654

    Article  CAS  Google Scholar 

  • Brain KR, Williams MH (1983) Evidence for an alternative route from sterol to sapogenin in suspension cultures from Trigonella foenumgraecum. Plant Cell Rep 2:7–10

    CAS  Google Scholar 

  • Brummitt RK (1992) Vascular plant families and genera. Royal Botanic Gardens, Kew, pp 595–602 Cerdon C, Rahier A, Taton M, Sauvaire Y (1995) Effect of diniconazole on sterol composition of roots and cell suspension cultures of fenugreek. Phytochemistrv 39:883–893

    Google Scholar 

  • Culafic L, Savikin-Fodulovic K, Grubisic D, Neskovic M (1999) Dioscorea balcanica Kosanin and D. caucasica Lipsky: in vitro culture and production of diosgenin. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 43. Medicinal and aromatic plants XI. Springer, Berlin Heidelberg New York, pp 85–104

    Google Scholar 

  • Dewick PM (1997) Medicinal natural products. A biosynthetic approach. Wiley, Chichester, pp 152–269

    Google Scholar 

  • Funk C, Brodelius P (1990) Influence of growth regulators and an elicitor on phenylpropanoid metabolism in suspension cultures of Vanilla planifolia. Phytochemistrv 29:845–848

    Article  CAS  Google Scholar 

  • Gamborg OL (1970) The effects of amino-acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol 45:372–375

    Article  PubMed  CAS  Google Scholar 

  • Girardon P, Sauvaire Y, Baccou JC, Bessiere JM (1986) Identification of 3-hydroxy-4,5-dimethyl2(5H)-furanone in aroma of fenugreek seeds (Trigonella foenum-graecum). Lebensm Wiss Technol 19:44–46

    CAS  Google Scholar 

  • Gupta KC (1974) Influence of auxins on growth and cytohistology of fenugreek (Trigonella foenum-graecum L.) calli. Cytobios 9:103–107

    PubMed  CAS  Google Scholar 

  • Heywood VH (1971) The Leguminosae-A systematic purview. In: Harborne JB, Boulter D, Turner BL (eds) Chemotaxonomy of the Leguminosae. Academic Press, , London pp 1–29

    Google Scholar 

  • Higgins JW (1976) A high-performance liquid chromatographic analysis of the benzoate esters of sapogenins isolated from Agave. J Chromatogr 121:329–334

    Article  CAS  Google Scholar 

  • Hooykaas PJJ, Klapwijk PM, Nuti MP, Schilperoort RA, Rörsch A (1977) Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent Agrobacteria and to Rhizobium ex planta. J Gen Microbiol 98:477–484

    Article  Google Scholar 

  • Ikenaga T, Oyama T, Muranaka T (1995) Growth and steroidal saponin production in hairy root cultures of Solanum aculeatissimum. Plant Cell Rep 14:413–417

    Article  CAS  Google Scholar 

  • Indrayanto G, Utami W, Syahrani A (1999) Costus speciosus (Koenig) J.E. Smith: in vitro cultures, micropropagation, and the production of diosgenin and other phytosteroids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 43. Medicinal and aromatic plants XI. Springer, Berlin Heidelberg New York, pp 57–77

    Google Scholar 

  • Iribbarren AM, Pomilio AB (1983) Components of Bauhinia candicans. J Nat Prod 46:752–753

    Article  Google Scholar 

  • Jain SC, Nag TN, Mohan S, Khanna P (1975) Effect of ascorbic acid on and its estimation in plant tissue cultures. Sci Cult 41:292–293

    CAS  Google Scholar 

  • Jain SC, Rosenberg H, Stohs SJ (1977) Steroidal constituents of Trigonella occulta tissue cultures. Planta Med 31:109–111

    Article  PubMed  CAS  Google Scholar 

  • Joshi JG, Handler P (1960) Biosynthesis of trigonelline. J Biol Chem 235:2981–2983

    PubMed  CAS  Google Scholar 

  • Kamal R, Yadav R (1992) Diosgenin and tigogenin from Trigonella polycerata plant parts and callus cultures. Indian Drugs 29:360–361

    CAS  Google Scholar 

  • Khanna P, Jain SC (1972) Effect of nicotinic acid on growth and production of trigonelline by Trigonella foenum-graecum L. tissue cultures. Indian J Exp Biol 10:248–249

    CAS  Google Scholar 

  • Khanna P, Jain SC (1973) Diosgenin, gitogenin and tigogenin from Trigonella f oenum-graecum tissue cultures. Lloydia 36:96–98

    CAS  Google Scholar 

  • Khanna P, Nag TN (1973) Production of amino acids in vitro tissue culture. Indian J Exp Bibl 11:310–311

    CAS  Google Scholar 

  • Khanna P, Jain SC, Bansal R (1975a) Effect of cholesterol on growth and production of diosgenin, gitogenin, tigogenin and sterols in suspension cultures. Indian J Exp Biol 13:211–213

    CAS  Google Scholar 

  • Khanna P, Bansal R, Jain SC (1975b) Effect of various hormones on production of sapogenins and sterols in Trigonella foenum-graecum L. suspension cultures. Indian J Exp Biol 13:582–583

    CAS  Google Scholar 

  • Kuhn A, Gerhard H (1943) The trigonellin and nicotinic acid contents of semen foenugraeci. Arch Pharm (Weinheim, Germany) 281:378–379

    Article  CAS  Google Scholar 

  • Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Int Plant Prop Soc Proc 30:421–427

    Google Scholar 

  • Lockwood GB, Brain KR (1976) Influence of hormonal supplementation on steroid levels during callus induction from seeds of Trigonella foenumgraecum. Phytochemistry 15:1655–1660

    Article  CAS  Google Scholar 

  • Mehra P. Yadav R, Kamal R (1996) Influence of nicotinic acid on production of trigonelline from Trigonella polycerata tissue culture. Indian J Exp Biol 34:1147–1149

    CAS  Google Scholar 

  • Merkli A (1996) Production de diosgénine par les racines transformées (hairy roots) de Trigonella foenum-graecum L. (Fabaceae). PhD Thesis, Univ Geneva, Switzerland

    Google Scholar 

  • Merkli A, Christen P, Kapetanidis I (1997) Production of diosgenin by hairy root cultures ot Trigonella foenum-graecum L. Plant Cell Rep 16:632–636

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Phvsiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Oncina R, Botia JM, Del Rio JA, Ortuño A (2000) Bioproduction of diosgenin in callus cultures of Trigonella foenum-graecum L. Food Chem 70:489–492

    Article  CAS  Google Scholar 

  • Ortuño A, Oncina R, Botia JM, Del Rio JA (1998) Distribution and changes of diosgenin during development of Trigonella foenum-graecum plants. Modulation by benzylaminopurine. Food Chem 63:51–54

    Article  Google Scholar 

  • Petit A, David C, Dahl DA, Ellis JG, Guyon P, Casse-Delbart F, Tempé J (1983) Further extension of the opine concept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190:204–214

    Article  CAS  Google Scholar 

  • Radwan SS, Kokate CK (1980) Production of higher levels of trigonelline by cell cultures of Trigonella foenum-graecum than by the differentiated plant. Planta 147:340–344

    Article  CAS  Google Scholar 

  • Rodriguez-Mendiola MA, Stafford A, Cresswell R, Arias-Castro C (1991) Bioreactors for growth of plant roots. Enzyme Microb Technol 13:696–702

    Article  Google Scholar 

  • Sauvaire Y, Baccou JC (1978) L’obtention de la diosgénine, (25R)-spirost-5-ène-3β-ol; problèmes de l’hydrolyse acide des saponines. Lloydia 41:247–256

    CAS  Google Scholar 

  • Sen B, Gupta S(1979) Differentiation in callus cultures of leaf of two species of Trigonella. Physiol Plant 45:425–428

    Article  Google Scholar 

  • Sharma AUGL, Khanna P (1977) Flavonoids from in vitro seedling callus culture of Trigonella foenum-graecum Linn. Indian J Pharm 39:142–143

    Google Scholar 

  • Shekhawat NS, Galston AW (1983) Mesophyll protoplasts of fenugreek (Trigonella foenumgraecum): isolation, culture and shoot regeneration. Plant Cell Rep. 2:119–121

    Article  CAS  Google Scholar 

  • Singh NN, Kokate CK, Tipnis HP (1981) A note on development of callus cultures of Trigonella foenum-graecum for diosgenin bioproduction. Indian Drugs 19:25–26, 33

    Google Scholar 

  • Trevelyan WE, Procter DP, Harrison JS (1950) Detection of sugars on paper chromatograms. Nature 166:444–445

    Article  PubMed  CAS  Google Scholar 

  • Trisonthi P, Baccou J-C, Sauvaire Y (1980) Essai d’amélioration de la production de sapogénines stéroïdiques par les tissus de fenugrec (Trigonella foenum-graecum) cultivés in vitro. CR Acad Sci Paris Ser D 291:357–360

    CAS  Google Scholar 

  • Tutin TG, Heywood VH, Burger NA, Moore DM, Valentine DH, Walters SM, Webb DA (eds) (1968) Flora Europaea, vol 2. Cambridge University Press, Cambridge, 152 pp

    Google Scholar 

  • Willis JC (1973) A dictionary of the flowering plants and ferns, 8th edn. Cambridge University Press, Cambridge, pp 1174–1175

    Google Scholar 

  • White PR (1943) A handbook of plant tissue culture. Jaques Cattell Press, Lancaster, Pennsylvania

    Book  Google Scholar 

  • Xu Z-H, Davey MR, Cocking EC (1982) Organogenesis form root protoplasts of the forage legumes Medicago sativa and Trigonella foenum-graecum. Z Pflanzenphysiol 107:231–235

    Google Scholar 

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© 2002 Springer-Verlag Berlin Heidelberg

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Christen, P. (2002). Trigonella Species: In Vitro Culture and Production of Secondary Metabolites. In: Nagata, T., Ebizuka, Y. (eds) Medicinal and Aromatic Plants XII. Biotechnology in Agriculture and Forestry, vol 51. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08616-2_17

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  • DOI: https://doi.org/10.1007/978-3-662-08616-2_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-07503-2

  • Online ISBN: 978-3-662-08616-2

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