Skip to main content

Nicotiana glauca: In Vitro Production of Pyridine Alkaloids and Other Secondary Metabolites

  • Chapter

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 33))

Abstract

Nicotiana glauca (family Solanaceae) is one of 21 species of Nicotiana, of which N. rustica and N. tabacum are the best known as sources of tobacco. N. glauca attains the greatest concentration and diversity in central Argentina where it is considered native. It is found less frequently in Bolivia where it also may be native. Indeed, N. glauca is represented to a greater or lesser degree in many warm temperate climates (Goodspeed 1954).

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aird ELH, Hamill JD, Robins RJ, Rhodes MJC (1988a) Chromosome stability in transformed root cultures and the properties of variant line of Nicotiana rustica hairy roots. In: Robins RJ, Rhodes MJC (eds) Manipulating secondary metabolism in culture. Cambridge Univ Press, Cambridge, pp 137–144

    Google Scholar 

  • Aird ELH, Hamill JD, Rhodes MJC (1988b) Cytogenetic analysis of hairy root cultures from a number of plant species transformed by Agrobacterium rhizogenes. Plant Cell Tissue Organ Cult 15: 47–57

    Article  Google Scholar 

  • Berlin J, Mollenschott C, Greidziak N, Erdogan S, Kuzovkina I (1990) Affecting secondary product formation in suspension and hairy root cultures — a comparison. In: Nijkamp HJJ, Van Der Plas LHW, Van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 763–768

    Chapter  Google Scholar 

  • Brodelius PE (1990) Transport and accumulation of secondary metabolites. In: Nijkamp HJJ, Van Der Plas LHW, Van Aartijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 567–576

    Chapter  Google Scholar 

  • Buitelaar RM, Langenhoff AAM, Heidstra R, Tramper J (1991) Growth and thiophene production by hairy root cultures of Tagetes patula in various two-liquid-phase bioreactors. Enzyme Microb Technol 13: 487–494

    Article  CAS  Google Scholar 

  • Buitelaar RM, Leenen EJTM, Geurtsen G, Groot E de, Tramper J (1993) Effects of the addition of XAD-7 and the elicitor treatment on growth, thiophene production, and excretion by hairy roots of Tagetes patula. Enzyme Microb Technol 15: 670–676

    Article  CAS  Google Scholar 

  • Corry JP, Reed WL, Curtis WR (1993) Enhanced recovery of Solvetivone from Agrobacterium transformed root cultures of Hyoscyamus muticus using integrated product extraction. Biotechnol Bioeng 42: 503–508

    Article  PubMed  CAS  Google Scholar 

  • DiCosmso F (1990) Strategies to improve yields of secondary metabolites to industrially interesting levels. In: Nijkamp HJJ, Van Der Plas LHW, Van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 717–725

    Google Scholar 

  • Dilorio AA, Cheetham RD, Weathers PJ (1992a) Growth of transformed roots in a nutrient mist bioreactor: reactor performance and evaluation. Appl Microbiol Biotechnol 37: 457–462

    Article  Google Scholar 

  • Dilorio AA, Cheetham RD, Weathers PJ (1992b) Carbon dioxide improves the growth of hairy roots cultured on solid medium and in nutrient mists. Appl Microbiol Biotechnol 37: 463–467

    Article  Google Scholar 

  • Duke JA (1985) Handbook of medicinal herbs. CRC Press, Boca Raton, pp 328–329

    Google Scholar 

  • Flores HE, Filner P (1985) Metabolic relationship of putrescine, GABA and alkaloids in cell and root cultures of Solanaceae. In: Neumann KH, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin; Heidelberg New York, pp 174–185

    Chapter  Google Scholar 

  • Flores HE, Hoy MW, Pickard JJ (1987) Secondary metabolites from root cultures. Trends Biotechnol 5: 64–69

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements for suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Goodspeed T H (1954) The genus Nicotiana. Chronica Botanica Company, Waltham, MA. pp 335–338

    Google Scholar 

  • Green KD (1991) Diagnostic evaluations for improved plant cell and root tissue culture. PhD Thesis, Univ Birmingham, UK

    Google Scholar 

  • Green KD, Thomas NH, Callow JA (1992) Product enhancement and recovery from transformed root cultures of Nicotiana glauca. Biotechnol Bioeng 39(2): 195–202

    Article  PubMed  CAS  Google Scholar 

  • Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Rep 5: 111–114

    Article  CAS  Google Scholar 

  • Hamill JD, Parr AJ, Rhodes MJC, Robins RJ, Walton NJ (1987) New routes to plant secondary products. Biotechnology 5: 800–804

    Article  CAS  Google Scholar 

  • Hamill JD, Robins RJ, Parr AJ, Evans DM, Furze JM, Rhodes MJC (1990) Over expressing a yeast ornithine decarboxylase gene in transgenic roots of Nicotiana rustica can lead to enhanced nicotine accumulation. Plant Mol Biol 15: 27–38

    Article  PubMed  CAS  Google Scholar 

  • Hawley GG (1977) The condensed chemical dictionary. Van Nostrand Reinhold, London, pp 59, 610

    Google Scholar 

  • Hilton MG, Rhodes MJC (1990) Growth and hyoscyamine production of hairy root cultures of Datura stramonium in modified stirred tank reactor. Appl Microbiol Technol 33: 132–138

    Article  CAS  Google Scholar 

  • Hilton MG, Wilson PDG, Robins RJ, Rhodes MJC (1988) Transformed root cultures — fermentation aspects. In: Robins RJ, Rhodes MJC (eds) Manipulating secondary metabolism in culture. Cambridge Univ Press, Cambridge, pp 239–245

    Google Scholar 

  • Institute of Food Research (IFR) (1991) Transformed roots: food ingredients, fine chemicals, pharmaceuticals. Bulletin, Agriculture and Food Research Council, UK

    Google Scholar 

  • Li SL, Green KD, Thomas NH, Ford-Lloyd BV (1994) Ongoing optimization of a root-tube bioreactor for transformed root cultures. 2nd UK Cong of Biotechnology, Society Chemical Industry, Brighton UK.

    Google Scholar 

  • Lyons I, Thomas NH, Callow JA (1988) The effect of N-methyl putrescine on growth and alkaloid production by transformed root culture of Nicotiana glauca. Poster display; PSE/SEB Int Symp, King’s College, Univ London

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Ogino T, Hiraoka N, Tabata M (1978) Selection of high nicotine producing cell lines of tobacco callus by single cell cloning. Phytochemistry 17: 1907

    Article  CAS  Google Scholar 

  • Ohta S, Yatazawa M (1989) Nicotiana tabacum L. (tobacco): in vitro production of nicotine. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 7. Medicinal and aromatic plants II. Springer, Berlin Heidelberg New York, pp 367–378

    Google Scholar 

  • Parr AJ, Hamill JD (1987) Relationship between Agrobacterium rhizogenes transformed hairy roots and intact, uninfected Nicotiana plants. Phytochemistry 26(12): 3241–3245

    Article  CAS  Google Scholar 

  • Parr AJ, Peerless ACJ, Hamill JD, Walton NJ (1988) Alkaloid production by transformed roots of Catharanthus roseus. Plant Cell Rep 7: 309–312

    Article  CAS  Google Scholar 

  • Payne GF, Shuler ML (1988) Selective adsorption of plant products. Biotechnol Bioeng 31: 922–928

    Article  PubMed  CAS  Google Scholar 

  • Payne GF, Payne NN, Shuler ML, Asada M (1988) In situ adsorption for enhanced alkaloid production by Catharanthus roseus. Biotechnol Lett 10 3: 187–192

    Article  CAS  Google Scholar 

  • Rhodes MJC, Hilton M, Parr AJ, Hamill JD (1986) Nicotine production by hairy root cultures of Nicotiana rustica: fermentation and product recovery. Biotechnol Lett 8: 415–420

    Article  CAS  Google Scholar 

  • Robins RJ, Rhodes MJC (1986) The stimulation of anthraquinone production by Cinchona ledgeriana cultures with polymeric adsorbents. Appl Microbiol Biotechnol 24: 35–41

    Article  CAS  Google Scholar 

  • Robins RJ, Hamill JD, Parr AJ, Smith K, Walton NJ, Rhodes MJC (1987) Potential use of nicotinic acid as a selective agent for isolation of high nicotine-producing lines of Nicotiana rustica hairy root cultures. Plant Cell Rep 6: 122–126

    CAS  Google Scholar 

  • Scragg AH, Cresswell RC, Ashton S, York A, Bond PA, Fowler MW (1989) Growth and alkaloid production in bioreactors by a selected Catharanthus roseus cell line. Enzyme Microb Technol 11: 329

    Article  CAS  Google Scholar 

  • Shimomura K, Sudo H, Saga H, Kamada H (1991) Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon. Plant Cell Rep 10: 282–285

    Article  CAS  Google Scholar 

  • Sim SJ, Chang HN (1993) Increased shikonin production by hairy roots of Lithospermum erythrorhizon in two phase bubble column reactor. Biotechnol Lett 15(2): 145–150

    Article  CAS  Google Scholar 

  • Snape JB, Thomas NH, Callow JA (1989) How suspension cultures of Catharanthus roseus respond to oxygen limitation: small-scale tests with applications to large-scale cultures. Biotechnol Bioeng 34: 1058–1062

    Article  PubMed  CAS  Google Scholar 

  • Taya M, Yoyama A, Kondo O, Kobayashi T (1989a) Growth characteristics of plant hairy roots and their cultures in bioreactors. Chem Eng Jpn 22(1): 84–89

    Article  Google Scholar 

  • Taya M, Yoyama A, Nomura R, Kondo O, Matsui C, Kobayashi T (1989b) Production of peroxidase with horseradish hairy root cells in a two step culture system. J Ferment Bioeng 67 1: 31–34

    Article  CAS  Google Scholar 

  • Toivonen L (1993) Utilization of hairy root cultures for production of secondary metabolites. Biotechnol Prog 9: 12–20

    Article  CAS  Google Scholar 

  • Toivonen L, Ojala M, Kauppinen V (1990) Indole alkaloid production by hairy root cultures of Catharanthus roseus: growth kinetics and fermentation. Biotechnol Lett 12: 519–524

    Article  CAS  Google Scholar 

  • Walton NJ, Belshaw NJ (1988) The effect of cadaverine on the formation of anabasine from lysine in hairy root cultures of Nicotiana hesperis. Plant Cell Rep 7: 115

    Article  CAS  Google Scholar 

  • Walton NJ, Robins RJ, Belshaw NJ, Parr AJ, Hamill JD, Rhodes MJC (1988) Perturbation of alkaloid biosynthesis in transformed root cultures of Solanaceous plants by metabolic precursors and their analogues. In: Robins RJ, Rhodes MJC (eds) Manipulating secondary metabolism in culture. Cambridge Univ Press, Cambridge, pp 73–78

    Google Scholar 

  • Wang HY (1983) Integrating biochemical separation and purification steps in fermentation processes. Ann NY Acad Sci 413: 313–321

    Article  PubMed  CAS  Google Scholar 

  • Wang HY, Kominek LA, Jost JL (1981) On line fermentation processes. In: Moo Young M, Robinson CW, Vezina C (eds) Advances in biotechnology I. Scientific and engineering principles. Pergamon, Oxford, pp 601–607

    Google Scholar 

  • Weathers PJ, Dilorio A, Cheetham R, O’Leary M (1990) Recovery of secondary metabolites with minimal loss of cell viability. In: Nijkamp HJJ, Van Der Plas LHW, Van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 582–586

    Chapter  Google Scholar 

  • White PR (1943) A handbook of plant tissue culture. Lancaster Press, Lancaster

    Book  Google Scholar 

  • Whitney PJ (1992) Novel bioreactors for the growth of roots transformed by Agrobacterium rhizogenes. Enzyme Microb Technol 14: 13–17

    Article  CAS  Google Scholar 

  • Wilson PDG, Hilton MG, Robins RJ, Rhodes MJC (1987) Fermentation studies of transformed root cultures. In: Moody GW, Baker PB (eds) Bioreactors and biotransformations. Elsevier, New York pp 38–51

    Google Scholar 

  • Wilson PDG, Hilton MG, Steer DC, Waspe CR, Robins RJ, Rhodes MJC (1988) Fermenter, International Patent Application WO 89/10958

    Google Scholar 

  • Wilson PDG, Hilton MG, Meehan PTH, Waspe CR, Rhodes MJC (1990) The cultivation of transformed roots from laboratory to pilot plant. In: Nijkamp HJJ, Van Der Plas LHW, Van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 700–705

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Green, K.D., Thomas, N.H. (1995). Nicotiana glauca: In Vitro Production of Pyridine Alkaloids and Other Secondary Metabolites. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants VIII. Biotechnology in Agriculture and Forestry, vol 33. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08612-4_18

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-08612-4_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08201-6

  • Online ISBN: 978-3-662-08612-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics