The effect of exogenous L-carnitine on biochemical parameters in serum and in heart of the hyperlipidaemic rat
In previous experiments we have demonstrated that L-carnitine administration is capable of reducing olive oil-induced lipidaemia in the rat. In the present study we determined the effect of L-carnitine on the levels of (acyl)carnitines in heart and serum in addition to its effect on serum levels of lipids and ketone bodies after olive oil gavage feeding. L-carnitine was found to reduce the level of myocardial long-chain acylcarnitine which was increased by the olive oil treatment. It also increased the levels of carnitine and acid soluble acylarnitines in both heart and serum. L-carnitine administration caused a clearcut decrease of olive oil-induced lipidaemia and ketonaemia. These effects of added L-carnitine strongly suggest that the stimulation of the β-oxidation in the mitochondria (at the expense of extra mitochondrial triglycerceride synthesis) is suboptimal after fat loading.
Key wordshyperlipidaemia triglycerides carnitines heart serum (in the rat).
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- 11.Konig B, McKaigney E, Contel S. Ross B (1978) Effect of a lipid load on blood and urinary carnitine in man. Clin Chim Acta 88: 121–125Google Scholar
- 13.Maccari F, Ramacci MT, Angelucci L (1980) Serum lipoprotein pattern in rats following fat load: modifications by L-carnitine. In: Noseda G, Lewin B, Paoletti R (eds) Diet and Drugs in Atherosclerosis, Raven Press, New York pp 15–21Google Scholar
- 15.Mellanby J, Williamson DH (1974) Acetoacetate and ß-hydroxybutyrate determination. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, Weinheim 4: pp 1836–1843Google Scholar
- 17.Pola P, Sav i L, Grilli M, Flore R, Serricchio M (1980) Carnitine in the therapy of dyslipidemic patients. Curr Ther Res 27 (2): 208–216Google Scholar
- 20.Trinder P (1969) serum cholesterol enzymatic analysis. Am Clin Biochem 6: 24–31Google Scholar
- 21.Wahlefeld AW (1974) Triglycerides determination after enzymatic hydrolysis. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, Weinheim 4: 1831–1835Google Scholar