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Zusammenfassung

Endotoxine sind integraler Bestandteil der äußeren Membran gramnegativer Bakterien wie Enterobakteriaceae, Neisserien und Chlamydien. Sie sind bei zahlreichen physiologischen Membranfunktionen beteiligt und sind essentiell für das Wachstum von Bakterien und deren Überleben. Endotoxine repräsentieren die hitzestabilen O-Antigene von Bakterien und definieren somit die Vielfalt der unterschiedlichen Serotypen. Schließlich zeigen Endotoxine ein großes Spektrum biologischer Aktivitäten nach der Injektion in Tieren und in In-vitro-Modellen [28].

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Literatur

  1. 1.
    Alpert G, Baldwin G, Thompson C et al. (1992) Limulus antipolysaccharide factor protects rabbits from meningococcal endotoxin shock. Infect Dis 165: 494–500CrossRefGoogle Scholar
  2. 2.
    Bazil V, Strominger, JL (1991) Shedding as a mechanism of down-modulation of CD 14 on stimulated human monocytes. J Immunol 147: 1567–1574PubMedGoogle Scholar
  3. 3.
    Desch CE, O’Hara P, Harlan J (1989) Anti-lipopolysaccharide factor from T. tridentatus inhibits lipopolysaccharide activation of cultured human endothelial cells. Infect Immun 57: 1612–1614PubMedGoogle Scholar
  4. 4.
    DiPadova FE, Brade H, Barclay R et al. (1993) A broadly cross-protective monoclonal antibody binding to E. coli and Salmonella lipopolysaccharide. Infect Immun 61: 3863–3872Google Scholar
  5. 5.
    Fletcher M, McKenna TM, Quance JL, Wainwright NR, Williams TJ (1993) Lipopolysaccharide detoxification by endotoxin neutralizing protein. J Surg Res 55: 147–154PubMedCrossRefGoogle Scholar
  6. 6.
    Fischer JC, Marra MN, Palardy JE, Marchbanks CR, Scott R, Opal SM (1994) Human neutrophil bactericidal/permeability-increasing protein reduces mortality rate from endotoxin challenge: A placebo-controlled study. Crit Care Med 22: 553–558CrossRefGoogle Scholar
  7. 7.
    Garcia C, Thompson C, Hammer B et al. (1994) Efficacy of a recombinant endotoxin neutralizing protein in rabbits with Escherichia coli sepsis. Circ Shock 42: 104–110PubMedGoogle Scholar
  8. 8.
    Garcia C, Saladino R, Thompson C et al. (1994) Effect of recombinant endotoxin-neutralizing protein on endotoxin shock in rabbits. Crit Care Med 22: 1211–1218PubMedCrossRefGoogle Scholar
  9. 9.
    Gazzano-Santoro H, Parent JB, Grinna L et al. (1992) High affinity binding of the bactericidal/permeability increasing protein and a recombinant amino terminal fragment to the lipid A region of lipopolysaccharide. Infect Immun 60: 4754–4761PubMedGoogle Scholar
  10. 10.
    Gazzano-Santoro H, Meszaros K, Birr C et al. (1994) Competition between rBPI23, a recombinant fragment of bactericidal/permeability-increasing protein, and lipopolysaccharide (LPS)-binding protein for binding to LPS and Gramnegative bacteria. Infect Immun 62: 1184–1191Google Scholar
  11. 11.
    Grunwald U, Krüger C, Schutt C (1993) Endotoxin-neutralizing capacity of soluble CD 14 is a highly conserved specific function. Circulatory Shock 39: 220–225PubMedGoogle Scholar
  12. 12.
    Hoess A, Watson S, Siber GR, Liddington R (1993) Crystal structure of an endotoxin-neutralizing protein from horseshoe crab, Limulus anti-LPS factor, at 1.5 A resolution. EMBO J 12: 3351–3356PubMedGoogle Scholar
  13. 13.
    Johnson KJ, Ward P, Goralnick BS, Osborn MJ (1977) Isolation from human serum of an inactivator of bacterial lipopolysaccharide. Am J Pathol 88: 559–574PubMedGoogle Scholar
  14. 14.
    Krüger C, Schutt C, Obertacke U et al. (1991) Serum CD 14 levels in polytraumatized and severely burned patients. Clin Exp Immunol 85: 297–301PubMedCrossRefGoogle Scholar
  15. 15.
    Kuppermann N, Nelson D, Saladino R et al. (1994) A comparison of a recombinant endotoxin neutralizing protein with a human monoclonal antibody to endotoxin for the treatment of E. coli sepsis in rats. J Infect Dis 170: 630–635CrossRefGoogle Scholar
  16. 16.
    Loppnow H, Libby P (1989) Adult human vascular endothelial cells express the IL-6 gene differentially in response to LPS or IL-1. Cell Immunol 122: 493–503PubMedCrossRefGoogle Scholar
  17. 17.
    Loppnow H, Libby P (1992) Functional significance of human vascular smooth muscle cell-derived interleukin 1 in paracrine and autocrine regulation pathways. Exp Cell Res 198: 283–290PubMedCrossRefGoogle Scholar
  18. 18.
    Loppnow H, Rietschel ET, Brade H et al. (1993) Lipid A precursor la (compound 406) and Rhodobacter capsulatus lipopolysaccharide: potent endotoxin antagonists in the human system in vitro. In: Levin J, Alving CR, Munford RS, Stutz PL (eds) Bacterial endotoxin: Recognition and effector machanisms. Elsevier, New York, pp 337–348Google Scholar
  19. 19.
    Marra MN, Wilde CG, Snable JL et al. (1990) Bactericidal/permeability-increasing protein has endotoxin neutralizing activity. J Immunol 144: 662–666PubMedGoogle Scholar
  20. 20.
    Marra MN, Wilde CG, Collins MS, Snable JL, Thornton MB, Scott RW (1992) The role of bactericidal/permeability-increasing protein as a natural inhibitor of bacterial endotoxins. J Immunol 148: 532–537PubMedGoogle Scholar
  21. 21.
    Marra MN, Thornton MB, Snable JL, Wilde CG, Scott RW (1994) Endotoxin-binding and-neutralizing properties of recombinant bactericidal/permeability increasing protein and monoclonal antibodies HA-1A and E5. Crit Care Med 22: 559–565PubMedCrossRefGoogle Scholar
  22. 22.
    Morita TS, Ohtsubo T, Nakamura T et al. (1985) Isolation and biological activities of Limulus anticoagulant (anti-lipopolysaccharide factor) which interacts with lipo-polysaccharide (LPS). J Biochem 97: 1611–1620PubMedGoogle Scholar
  23. 23.
    Muta T, Miyata T, Tokunaga F, Nakamura T, Iwanaga S (1987) Primary structure of anti-lipopolysaccharide factor from American horseshoe crab, Limulus polyphemus. J Biochem 101: 1321–1330PubMedGoogle Scholar
  24. 24.
    Nathan CF (1987) Secretory products of macrophages. J Clin Invest 79: 319–324PubMedCrossRefGoogle Scholar
  25. 25.
    Ooi CE, Weiss J, Doerfler ME, Eisbach P (1991) Endotoxin-neutralizing properties of the 25 kD N-terminal fragment and a newly isolated 30 kD C-terminal fragment of the 55–60 kD bactericidal/permeability-increasing protein of human neutrophils. J Exp Med 174: 649–655PubMedCrossRefGoogle Scholar
  26. 26.
    Pugin J, Schürer-Maly CC, Leturcq D, Moriarty A, Ulevitch RJ, Tobias PS (1993) Lipopolysaccharide activation of human endothelial and epithelial cells is mediated by lipopolysaccharide-binding protein and soluble CD 14. Proc Natl Acad Sei USA 90:2744–2748CrossRefGoogle Scholar
  27. 27.
    Rietschel ET, Brade H (1992) Bacterial endotoxins. Sei Am 267: 54–61Google Scholar
  28. 28.
    Rietschel ET, Kirikae T, Feist W et al. (1991) Molecular aspects of the chemistry and biology of endotoxin. In: Sies H, Flohe L, Zimmer G (eds) 1991. Molecular aspects of inflammation. Springer, Berlin Heidelberg New York Tokyo, pp 207–231CrossRefGoogle Scholar
  29. 29.
    Saladino R, Garcia CT, Thompson C et al. (1991) Assessment of intravenous endotoxin neutralizing protein in a rabbit model. Pediatr Res 29: 33AGoogle Scholar
  30. 30.
    Schletter J, Brade H, Krüger C, Loppnow H, Rietschel ET, Flad HD, Ulmer AJ (1994) Binding of lipopolysaccharide to an 80 KDa membrane protein of human cells is mediated by serum factors. Immunobiol 191: 273Google Scholar
  31. 31.
    Schumann RR, Leong SR, Flaggs GW, Gray PW, Wright SD, Mathison JC, Tobias PS, Ulevitch RJ (1990) Structur and function of lipopolysaccharide binding protein. Science 249: 1429–1431PubMedCrossRefGoogle Scholar
  32. 32.
    Schumann RR, Lamping N, Kirschning C, Knopf HP, Hoess A, Herrmann F (1994) Lipopolysaccharide binding protien: Its role and therapeutic potential in inflammation and sepsis. Biochem Soc Transact 22: 80–83Google Scholar
  33. 33.
    Schumann RR, Rietschel ET, Loppnow H (in press) The role of CD14 and lipopolysaccharide binding protein [LBP] in the activation of different cell types by endotoxin. Med Mikrobiol ImmunolGoogle Scholar
  34. 34.
    Schutt C, Schilling T, Krüger C (1991) sCD14 prevents endotoxin induced oxidative burst response of human monocytes. Allerg Immunol 37: 159–164Google Scholar
  35. 35.
    Schutt C, Schilling T, Grunwald U, Schönfeld W, Krüger C (1992) Endotoxinneutraliz-ing capacity of soluble CD14. Res Immunol 143: 71–78PubMedCrossRefGoogle Scholar
  36. 36.
    Seydel U, Brandenburg K, Rietschel ET (1994) A case for an endotoxic conformation. In: Levin J et al. (eds) Bacterial endotoxins: Basic science to anti-sepsis strategies. Wiley/Liss, New York, pp 17–30Google Scholar
  37. 37.
    Tanaka S, Nakamura T, Morita T, Iwanaga S (1982) Limulus anti-lipopolysaccharide factor: An anticoagulant which inhibits the endotoxinmediated activation of limulus coagulation system. Biochem Biophys Res Commun 105: 717–723PubMedCrossRefGoogle Scholar
  38. 38.
    Theofan G, Horwitz AH, Williams ER, et al. (1994) An amino-terminal fragment of human lipopolysaccharide-binding protein retains lipid A binding but not CD 14-stimulatory activity. J Immunol 152: 3623–3629PubMedGoogle Scholar
  39. 39.
    Tobias PS, Mathison JC, Ulevitch RJ (1988) A family of lipopolysaccharide binding protiens involved in responses to Gram-negative sepsis. J Biol Chem 263: 13479–13481PubMedGoogle Scholar
  40. 40.
    Wainwright NR, Müller RJ, Paus E et al. (1990) Endotoxin binding and neutralizing activity by a protein from Limulus polyphemus. In: Novotny A (ed) Cellular and molecular aspects of endotoxin reaction. Elsevier, New York, pp 315–325Google Scholar
  41. 41.
    Warren HS, Glennon ML, Wainwright N et al. (1992) Binding and neutralization of endotoxin by limulus antilipopolysaccharide factor. Infect Immun 60: 2506–2513PubMedGoogle Scholar
  42. 42.
    Weinstein SL, Gold MR, DeFranco AL (1991) Bacterial lipopolysaccharide stimulation protein tyrosine phosporylation in macrophages. Proc Natl Acad Sei USA 88: 4148–4152CrossRefGoogle Scholar
  43. 43.
    Weiss J, Eisbach P, Olsson I et al. (1978) Purification and characterization of a potent bactericidal and membrane active protein from the granules of human polymorphnu-clear leukocytes. J Biol Chem 253: 2664–2672PubMedGoogle Scholar
  44. 44.
    Würfel M, Kunitake ST, Lichenstein H, Kane JP, Wright SD (1994) Lipopolysaccharide (LPS)-binding protein is carried on lipoproteins and acts as a cofactor in the neutralization of LPS. J Exp Med 180: 1025–1035PubMedCrossRefGoogle Scholar
  45. 45.
    Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison LC (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding proteins. Science 249: 1431–1433PubMedCrossRefGoogle Scholar
  46. 46.
    Zähringer U, Lindner B, Rietschel ET (1994) Molecular structure of Lipid A, the endotoxic center of bacterial lipopolysaccharides. Adv Carbohyd Chem Biochem 50:211–276CrossRefGoogle Scholar
  47. 47.
    Ziegler-Heitbrock HWL, Ulevitch RJ (1993) CD14: Cell surface receptor and differentiation marker. Immunol Today 14: 121–125PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • P. Zabel

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