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Immunhistologische Techniken

  • H. Kutzner
  • G. Palmedo

Zusammenfassung

Im formalinfixierten, in Paraffin eingebetteten Gewebe hängt der Erfolg immunhistochemischer Färbungen von verschiedenen Bedingungen ab:
  • Die nachzuweisenden antigenen Determinanten (Epitope) müssen die Formalinfixierung und den nachfolgenden Einbettungsvorgang unbeschadet überstehen oder sie sollten nach fixierungsbedingter Alteration ihrer tertiären Proteinstruktur rekonstituierbar sein (Antigendemaskierung).

  • Erforderlich sind Antikörper, die sich auch im fixierten Gewebe spezifisch an Epitope binden können.

  • Zum Nachweis der spezifischen Antikörperbindung sind sensitive Detektionssysteme notwendig, die lichtmikroskopisch erkennbare, permanente Farbsignale geben.

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© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • H. Kutzner
  • G. Palmedo

There are no affiliations available

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