Micropropagation of Aegle marmelos Corr. (Bael)

  • M. Hossain
  • R. Islam
  • O. I. Joarder
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 39)


Aegle marmelos, Corr., commonly known as bael, is medium-sized slow-growing deciduous spiny woody fruit tree of the tropics (Fig. 1). It belongs to the family Rutaceae, in which polyembryony is a common phenomenon (Melchior 1964; Rangaswamy 1981). Its flowers are sweet-scented and bloom during the spring. It takes 10–12 months from flowering to fruit ripening. Fruits are large, globose, ovoid or pyriform, green when unripe and turn yellow-brown when ripe. Seeds are embedded in aromatic, sweet, thick pulp. The testa is woody and the seeds are oblong, compressed; the embryo has a thick fleshy cotyledon.


Leaf Explants Adventitious Shoot Shoot Induction Embryo Axis Plant Cell Tissue Organ Cult 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Arya HC (1986) Clonal multiplication of tree species in the Thar desert through tissue culture. For Ecol Manage 16: 201–208CrossRefGoogle Scholar
  2. Arya HC, Ramawat KC, Suthar KC (1981) Culture and differentiation of plants of economic importance II. Aegle marmelos L. J Indian Bot Soc 60: 134–137Google Scholar
  3. Bajaj YPS (1992) Biotechnology in agriculture and forestry, vol 18. High-tech and micro-propagation IL Springer, Berlin Heidelberg New YorkGoogle Scholar
  4. Baker BS, Bhatia SK (1993) Factors affecting adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga). Plant Cell Tissue Organ Cult 35: 273 278Google Scholar
  5. Biswas K, Ghosh A (ed) (1973) Bharatio bonoushadhi, vol I. Calcutta University Press, CalcuttaGoogle Scholar
  6. Bonga JM (1987) Clonal propagation of mature tree: problems and possible solutions. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol I. Martinus Nijholft, Dordrecht, pp 249–271Google Scholar
  7. Button J, Kochba J (1977) Tissue culture in the Citrus industry. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 70–92Google Scholar
  8. Chalupa V (1990) Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L.) and linden (Tiiia cordata Mill.). Plant Cell Rep 9: 398 401Google Scholar
  9. Clair STJB, Kleinschmidt J, Svolba J (1985) Juvenility and serial vegetative propagation of Norway spruce clones (Picea abies Karst.). Silvae Genet 34: 42–48Google Scholar
  10. Fasalo F, Zimmerman RH, Fordham I (1989) Adventitious shoot formation on excised leaves of in vitro-grown shoots of apple cultivars. Plant Cell Tissue Organ Cult 16: 75 87Google Scholar
  11. Heywood VA, Chant AR (eds) (1982) Popular encyclopedia of plants. Cambridge University Press, CambridgeGoogle Scholar
  12. Hooker JD (ed) (1875) The flora of British India, vol 1. Reeve, KentGoogle Scholar
  13. Hossain M, Karim MR, Islam R, Joarder OI (1993) Plant regeneration from nucellar tissues of Aegle marmelos through organogenesis. Plant Cell Tissue Organ Cult 34: 199–203CrossRefGoogle Scholar
  14. Hossain M, Islam R, Karim MR, Joarder OI, Biswas BK (1994a) Regeneration of plantlets from in vitro-cultured cotyledon of Aegle maremelos Corr. (Rutaceae). Sci Hortic 57: 315 321Google Scholar
  15. Hossain M, Karim MR, Islam R, Joarder OI (1994b) Production of plantlets from Aegele marmelos nucellus callus. Plant Cell Rep 13: 570–573CrossRefGoogle Scholar
  16. Hu CY, Sussex IM (1971) In vitro development of embryoids on cotyledons of Ilex aquifolium. Phytomorphology 21: 103–107Google Scholar
  17. Hutchinson JF (1981) Tissue culture and propagation of forest trees. In: Rao AN (ed) Proc Symp Tissue culture of economically important plants. COSTED, Singapore, pp 113–120Google Scholar
  18. Islam R, Karim MR, Hossain M, Joarder CM (1992) Plant regeneration from leaf-derived callus in Aegle marmelos Corr. Plant Tissue Cult 2: 55–59Google Scholar
  19. Islam R, Hossain M, Joarder OI, Karim MR (1993a) Adventitious shoot formation on excised leaf explants of in vitro-grown seedlings of Aegle marmelos Corr. J Hortic Sci 68: 495–498Google Scholar
  20. Islam R, Karim MR, Hague A, Hossain M, Joarder OI (1993b) High efficiency plant regeneration from cotyledon cultures of Aegle marmelos Corr. Plant Tissue Cult 3: 107–110Google Scholar
  21. Isalm R, Karim MR Rahman SM, Hossain M, Joarder OI (1994) Plant regeneration from excised cotyledon of Aegle marmelos Corr. Pak J Bot 26: 393–396Google Scholar
  22. Jha T, Sen SK (1992) Micropropagation of an elite Darjeeling tea clone. Plant Cell Rep 11: 101 104Google Scholar
  23. Kouidcr M, Korban SS, Skirivin RM, Jung H (1985) The relationship of apple embryos and their cotyledon to maturity, dormancy and the potential to form shoots in vitro. J Am Soc Hortic Sci 110: 95–96Google Scholar
  24. Melchior H (ed) (1964) Engler’s Syllabus der Pflanzenfamilien, vol 2. Angiospermen. Kolassee, BerlinGoogle Scholar
  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  26. Ozean S, Barghchi M, Firek S, Draper J (1992) High frequency shoot regeneration from immature cotyledons of pea (Pisum sativum L.). Plant Cell Rep 11: 44–47Google Scholar
  27. Purseglove JW (ed) (1968) Tropical crops — dicotyledons I. Longman London, HarlowGoogle Scholar
  28. Rangaswamy NS (1981) Nucellus as an experimental system in basic and applied tissue culture research. In: Rao AN (ed) Proc Symp Tissue culture of economically important plants. COSTED, Singapore, pp 269–286Google Scholar
  29. Samad AG (ed) (1966) Udbhid Shamiksha. Bangla Academy, Dhaka, BangladeshGoogle Scholar
  30. Sharma D (ed) (1980) Shyamolee Nisharga. Bangla Academy, Dhaka, BangladeshGoogle Scholar
  31. Singh UP (1963) Raising nucellar seedlings of some Rutaceae in vitro. In: Maheshwari P, Ran-gaswamy NS (eds) Symp Plant tissue and organ culture. Int Soc Plant Morphologists. University of Delhi, Delhi, pp 275 277Google Scholar
  32. Singh UR, Panday IC, Prased RS (1976) Propagation of bad (Aegle marmelos Corr.). Punjab Hortic J 16: 57–59Google Scholar
  33. Zaman ME (ed) (1988) Bangladesher Phaller Chash. Bangla Academy, Dhaka, BangladeshGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • M. Hossain
    • 1
  • R. Islam
    • 1
  • O. I. Joarder
    • 1
  1. 1.Department of BotanyUniversity of RajshahiRajshahiBangladesh

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