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Micropropagation of Spinacia oleracea L. (Spinach)

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High-Tech and Micropropagation V

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 39))

Abstract

Spinach (Spinacia oleracea L.; Fig. 1) is a hardy cool-season annual crop classified as a potherb vegetable. It produces a rosette of fleshy edible leaves during the vegetative stage of its life cycle. The reproductive stage, triggered by long-day photoperiods, is marked by stem elongation and branching to form flower stalks. Spinach is mostly a dioecious plant but monoecious plants with varying proportions of male (staminate) and female (pistillate) flowers may also exist (Ryder 1979). Spinach is a diploid with chromosome number 2n = 2x = 12. Its sex determination is hypothesized to be controlled by a monogenic system in which a single gene with three alleles (Y, Xm, and X) appear to explain the existence of monoecious plants in this dioecious species (Janick and Stevenson 1954, 1955).

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References

  • Al-Khayri JM (1991) In vitro regeneration, sex alteration, genetic transformation, and DNA isolation in spinach (Spinacia oleracea L.). PhD Dissertation, University of Arkansas, Fayetteville, pp 131–147

    Google Scholar 

  • Al-Khayri JM (1995) Genetic transformation in Spinacia oleracea L. (spinach). In: Bajaj YPS (cd) Biotechnology in agriculture and forestry, vol 34. Plant protoplasts and genetic engineering VI. Springer, Berlin Heidelberg New York pp 279–288

    Google Scholar 

  • Al-Khayri JM, Huang EH, Morelock TE (1989) Regeneration of spinach. In Vitro 25:26 Al-Khayri JM, Huang FH, Morelock TE (1990) Effect of agar, Gelrite, and IBA on rooting of in vitro spinach shoots. In Vitro 26: 67A

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE (1991a) Regeneration of spinach (Spinacia oleracea L. cv. Fall Green) from leaf callus. HortScience 26: 913 914

    Google Scholar 

  • Al-Khayri JM, Huang F14, Morelock TE (1991b) Micropropagation of spinach. Arkansas Farm Res 40 (1): 7

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE, Busharar TA (1991e) Spinach sex modification and seed production in vitro. Arkansas Farm Res 40 (6): 3–4

    Google Scholar 

  • Al-Khayri JM, Huang EH, Morelock TE, Busharar TA, Gbur EE (199ld) Genotype-dependent response of spinach cultivars to in vitro callus induction and plant regeneration. Plant Sei 78: 121–127

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE, Busharar TA, Lane FE (1991e) In vitro flowering in regenerated shoots of spinach (Spinacia oleracea L.). Hort Science 26: 1422

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE, Busharar TA (1992a) In vitro seed production from sex- modified male spinach plants regenerated from callus cultures. Sci Hortic 52: 277 282

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE, Busharar TA (1992b) Stimulation of shoot regeneration in spinach callus by gibberellic acid. HortScience 27: 1046

    CAS  Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE, Busharar TA (1992e) Spinach tissue culture improved with coconut water. HortScience 27: 357–358

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE, Busharar TA (1992d) In vitro plant regeneration of spinach (Spinacia oleracea L.) from mature seed-derived callus. In Vitro 28P: 64–66

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE, Zhang HT (1992e) Transient gene expression in spinach callus transformed with Agrohacterium humc/aciens. HortScicnce 27: 621

    Google Scholar 

  • Al-Khayri JM, Huang FH, Morelock TE (1993a) Spinach regeneration from cell suspension culture. In Vitro 29A: 71

    Google Scholar 

  • Al-Khayri JM, Miles RW, Huang EH, Morelock TE, Stewart JMcD (1993b) Expression of the GUS gene in Agrobacterium tumcfaciens-transformed spinach plants. HortScience 28: 498

    Google Scholar 

  • Al-Khayri JM, Miles RW, Huang FH, Morelock TE, Stewart JMcD (1994) Leaf disk transformation and regeneration of transgenic spinach. Ark Farm Res 43: 10 11

    Google Scholar 

  • Asami S, Hara-Nishimura I, Nishimura M, Akazawa T (1985) Translocation of photosynthales into vacuoles in spinach leaf protoplasts. Plant Physiol 77: 963 968

    Google Scholar 

  • Asari J, Okuse 1 (1993) Studies on the callus formation and growth of young seedling explants in spinach (Spinacia oleracea L.). Bull Fac Agric Hirosaki Univ 56: 23–32

    CAS  Google Scholar 

  • Benbadis A, Davy-de-Virville J (1982) Effects of polyethylene glycol treatment used for protoplast fusion and organelle transplantation on the functional and structural integrity of mitochondria isolated from spinach leaves (Spinacia oleracea). Plant Sci Lett 26: 257 264

    Google Scholar 

  • Brown JA, Fry SC (1993) Novel O-D-gaIacturonoy1 esters in the pectic polysaccharides of suspension cultured plant cells. Plant Physiol 103: 993 999

    Google Scholar 

  • Chailakhyan MKh, Khrianin VN (1987a) Effect of growth regulators and role of roots in sex expression in spinach plants. Planta 142: 207–210

    Article  Google Scholar 

  • Chailakhyan MKh, Khrianin VN (1987h) Sexuality in plants and ils hormonal regulation. Thimann KV (cd), Loroch V (translator). Springer, Berlin Heidelberg New York, 159 pp

    Chapter  Google Scholar 

  • Correll JC, Morelock TE, Black MC, Koike ST, Brandenberger LP, Dainello F,I (1994) Economically important diseases of spinach. Plant Dis 78: 653–660

    Article  Google Scholar 

  • Culafié (1973) Induction of flowering of isolated Spinacia oleracea L. buds in sterile culture. Bull Inst Jard Bot Univ Beograd 8: 53 56

    Google Scholar 

  • Culafié L, Neskovié M (1980) Effect of growth substances on flowering and sex expression in isolated apical buds of Spinacia oleracea. Physiol Plant 48: 588–591

    Article  Google Scholar 

  • Culafié L, Grubisie D, Neskovié M (1982) Endogenous gibberellin-like substances and inhibitors in callus tissue of Spinacia oleracea L. Bull Inst Jard Bot Univ Beograd 15: 29–35

    Google Scholar 

  • Culafié L, Konjevié R, Neskovié M (1983) Flowering of in vitro grown spinach shoots in the presence of the herbicide Sandoz 9789. Biol Plant 25: 155–157

    Article  Google Scholar 

  • Dalton CC, Street HE (1976) The role of the gas phase in the greening and growth of illuminated cell suspension cultures of spinach (Spinacia oleracea L.). In Vitro 12: 485–494

    Google Scholar 

  • Dalton CC, Street HE (1977) The influence of applied carbohydrates on the growth and greening of cultured spinach (Spinacia oleracea L.). Plant Sci Lett 10: 157–164

    Article  CAS  Google Scholar 

  • Foyer CH (1990) The effect of sucrose and mannose on cytoplasmic protein phosphorylation, sucrose phosphate synthetase activity and photosynthesis in leaf protoplasts from spinach. Plant Physiol Biochem 28: 151–160

    CAS  Google Scholar 

  • Heimann K, Kreimer G, Melkonian M, Latzko E (1987) Light-induced Cat+ influx into spinach protoplasts. Naturforsch Sec C Biosci 42: 283–287

    CAS  Google Scholar 

  • Hodgson RAJ, Rose RJ (1984) Fusion of spinach mesophyll protoplasts with carrot root parenchyma protoplasts and the effect of spinach chloroplasts. J Plant Physiol 115: 69–78

    Article  PubMed  CAS  Google Scholar 

  • Janick J, Stevenson EC (1954) A genetic study of the heterogametic nature of the staminate plants in spinach (Spinacia oleracea L.). Proc Am Soc Hortic Sci 63: 444–446

    Google Scholar 

  • Janick J, Stevenson EC (1955) Genetics of the monoecious character in spinach. Genetics 40: 429437

    Google Scholar 

  • Komai F, Okuse I (1993) The varietal differences in the plantlet regeneration from root explants of young seedlings in spinach (Spinacia oleracea L.). J Jpn Soc Hortic Sci 62 (Suppl 2): 260–261

    Google Scholar 

  • Kondo K, Nadamitsu S, Tanaka R, Taniguchi K (1991) Micropropagation of Spinacia oleracea L. through culture of shoot primordia. Plant Tissue Cult Lett 8: 1–4

    Article  Google Scholar 

  • Mii M, Okuda K, Iizuka M (1987) Plant regeneration from hypocotyl segments of Spinacia oleracea. Jpn J Breed 37 (Suppl 2): 24–25

    Google Scholar 

  • Mii M, Nakano M, Okuda K, Iizuka M (1992) Shoot regeneration from spinach hypocotyl segments by short term treatment with 5,6-dichloro-indole-3-acetic acid. Plant Cell Rep 11: 5861

    Article  Google Scholar 

  • Molvig L, Rose RJ (1994) A regeneration protocol for Spinacia oleracea L. using gibberellic acid. Aust J Bot 42: 763–769

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nakagawa H, Tanaka H, Oba T, Ogwa N, Iizuka M (1985) Callus formation from protoplasts of cultured Spinacia oleracea cells. Plant Cell Rep 4: 148–150

    Article  Google Scholar 

  • Neskovié M, Culafié L (1988) Spinach (Spinacia oleracea L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 6. Crops I1. Springer, Berlin Heidelberg New York, pp 370–385

    Google Scholar 

  • Neskovié M, Radojevié L (1973) The growth of and morphogenesis in tissue cultures of Spinacia oleracea L. Bull Inst Jard Bot Univ Belgrade 8: 35–37

    Google Scholar 

  • Neskovié M, Petrovié J, Radojevié L, Yujiéie R (1977) Stimulation of growth and nucleic acid biosynthesis at low concentration of abscisic acid in tissue culture of Spinacia oleracea. Physiol Plant 39: 148–154

    Article  Google Scholar 

  • Nishimura M, Akazawa T (1975) Photosynthetic activities of spinach leaf protoplasts. Plant Physiol 55: 712–716

    Article  PubMed  CAS  Google Scholar 

  • Nishimura M, Graham D, Akazawa T (1976) Isolation of intact chloroplasts and other cell organelles from spinach leaf protoplasts. Plant Physiol 58: 309–314

    Article  PubMed  CAS  Google Scholar 

  • Nishimura M, Douce R, Akazawa T (1985) A simple method for estimating intactness of spinach leaf protoplasts by glycolate oxidase assay. Plant Physiol 78: 343–346

    Article  PubMed  CAS  Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorophology 19: 389–404

    Google Scholar 

  • Ohlrogge JB, Kuhn DH, Stumpf PK (1979) Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea. Proc Natl Acad Sci USA 76: 1194–1198

    Article  PubMed  CAS  Google Scholar 

  • Okuse I (1994) Influence of the sexuality in flower stalk explants on the callus formation and growth in spinach (Spinacia oleracea L.). Bull Fac Agric Hirosaki 57: 55–62

    CAS  Google Scholar 

  • J.M. Al-Khayri: Micropropagation of Spinacia oleracea L. (Spinach) Rathnam CKM, Zilinskas BA (1977) Reversal of 3-(3,4-dichlorophenyl)-1, 1-dimethylurca inhibition of carbon dioxide fixation in spinach chloroplasts and protoplasts by dicarboxylic acids. Plant Physiol 60: 51 53

    Google Scholar 

  • Robinson SP, Walker DA (1979) Rapid separation of the chloroplast and cytoplasmic fractions from intact leaf protoplasts. Arch Biochem Biophys 196: 319 323

    Google Scholar 

  • Rose RJ (1980) Factors that influence the yield, stability in culture and cell wall regeneration of spinach mesophyll protoplasts. Aust J Plant Physiol 7: 713 725

    Google Scholar 

  • Ryder EJ (1979) Leafy salad vegetables. AVI Publishing Company, Westport, pp 195 227 Santakumari M, Berkowitz GA (1989) Protoplast volume: water potential relationship and bound water fraction in spinach leaves. Plant Physiol 91: 13 18

    Google Scholar 

  • Sasaki H (1989) Callus and organ formation tissue cultures of spinach (Spinacia oleracea L.). J Jpn Soc Hortic Sci 58: 149–153

    Article  Google Scholar 

  • Sasaki H, Nakai S, Kubouchi H (1986) Organ formation of spinach hypocotyl tissues cultured in vitro. J Hokkaido Univ Educ (II B) 37: 49–56

    Google Scholar 

  • Sasaki H, Saito Y, Yada H (1987) Adventitious bud formation of spinach hypocotyl tissue cultured in vitro. J Hokkaido Univ Educ (11 B) 38: 1 9

    Google Scholar 

  • Sasaki H, Ohta M, Ono M (1989) Effect of gibberellin concentration and cultivar on adventitious bud formation of spinach hypocotyl tissue cultured in vitro. J Hokkaido Univ Educ (II 13) 40: 73 80

    Google Scholar 

  • Sasaki H, Arato T, Takahashi K, Kobori T (1994) Regeneration of spinach plant from hypocotyl tissue cultured in vitro. J Hokkaido Univ Educ (II B) 45: 31 35

    Google Scholar 

  • Satoh S, Fujü T (1985) Presence of serine enzymes in endoplasmic reticulum of spinach callus. Plant Cell Physiol 26: 1037 1044

    Google Scholar 

  • Satoh T, Abe T, Sasahara T (1992) Plant regeneration from hypocotyl-derived calli of spinach (Spinacea oleracea L.) and anatomical characteristics of regenerating calli. Plant Tissue Cult Lett 9: 176–183

    Article  CAS  Google Scholar 

  • Sticher L, Pend C, Greppin H (1981) Calcium requirement for the secretion of peroxidases by plant cell suspensions. J Cell Sci 48: 345–353

    PubMed  CAS  Google Scholar 

  • Swiader JM, Ware GE, McCollum JP (1992) Producing vegetable crops. Interstate Publishers, Danville, pp 459–476

    Google Scholar 

  • Thompson AE (1955) Methods of producing first generation hybrid seeds in spinach. Mem Cornell Univ Agric Exp Stn 336: 1–48

    Google Scholar 

  • White PR (1943) A handbook of plant tissue culture. J Cattell, Lancaster, pp 39, 43, 433

    Google Scholar 

  • Wirtz W, Stitt M, Heldt HW (1980) Enzymic determination of metabolites in the subcellular compartments of spinach protoplasts. Plant Physiol 66: 187–193

    Article  PubMed  CAS  Google Scholar 

  • Xiao X-G, Branchard M (1993) Embryogenesis and plant regeneration of spinach (Spinacia (deracea L.) from hypocotyl segments. Plant Cell Rep 13: 69 71

    Google Scholar 

  • Zdravkovié-Korae S, Neskovie M (1993) Somatic embryogenesis in spinach (Spinacia oleracea L.). Arch Biol Sci Belgrade 45:57P 58 P

    Google Scholar 

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Author notes

  1. J. M. Al-Khayri

    Present address: Date Palm Research Center, King Faisal University, P.O. Box 400, Al-Hassa, 31982, Saudi Arabia

Authors and Affiliations

  1. Department of Horticulture, University of Arkansas, Fayetteville, Arkansas, 72701, USA

    J. M. Al-Khayri

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  1. J. M. Al-Khayri
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Editors and Affiliations

  1. New Friends Colony, A-137, 110065, New Delhi, India

    Y. P. S. Bajaj

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© 1997 Springer-Verlag Berlin Heidelberg

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Al-Khayri, J.M. (1997). Micropropagation of Spinacia oleracea L. (Spinach). In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation V. Biotechnology in Agriculture and Forestry, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-07774-0_12

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  • DOI: https://doi.org/10.1007/978-3-662-07774-0_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08269-6

  • Online ISBN: 978-3-662-07774-0

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