In Vitro Culture and Micropropagation of Ryegrass (Lolium spp.)

  • J. Creemers-Molenaar
  • L. J. Beerepoot
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 19)

Abstract

The ryegrasses (Lolium spp.) can be classified into eight different species. However, since these species are partly or fully interfertile, this classification is not always unequivocal (Breese and Tyler 1986). Two of these species, perennial ryegrass (Lolium perenne L.) and Italian ryegrass (Lolium multiflorum Lam.) are agriculturally very important. Strictly annual forms of Italian ryegrass are often referred to as Westerwolds ryegrass (Lolium multiflorum Lam. ssp. westerwoldicum). Hybrid ryegrass (Lolium × hybridum Housskn.) is derived from artificial or naturally occurring crosses between Italian and perennial ryegrass. Perennial and Italian ryegrass are diploid (2n = 14), outbreeding and self-incompatible species. No naturally occurring tetraploids have ever been found. However, by means of colchicine treatment, tetraploid varieties (4n = 28) have been made artificially (Wit 1959).

Keywords

Sucrose Maize Chlorine Sulfuric Acid Manure 

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References

  1. Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technol 4: 1087–1090CrossRefGoogle Scholar
  2. Ahloowalia BS (1975) Regeneration of ryegrass plants in tissue culture. Crop Sci 15: 449–452 Ahloowalia BS (1983) Spectrum of variation in somaclones of triploid ryegrass. Crop Sci 23: 1141–1147Google Scholar
  3. Ahuja PS, Pental D, Cocking EC (1982) Plant regeneration from leaf base callus and cell suspensions of Triticum aestivum. Z Pflanzenzucht 89: 13 144Google Scholar
  4. Atkin RK, Barton GE (1973) The establishment of tissue cultures of temperate grasses. J Exp Bot 24: 689–699CrossRefGoogle Scholar
  5. Bajaj YPS (1989) Genetic engineering and in vitro manipulation of plant cells–technical advances. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 9. Plant protoplasts and genetic engineering II. Springer, Berlin Heidelberg New York, pp 1–25Google Scholar
  6. Bante I, Sonke T, Tandler RF, van den Bruel AM, Meijer EM (1990) Anther culture of Lolium perenne L. and Lolium multiflorum L. Acta Bot Neerl 39 (1): 103Google Scholar
  7. Bante I, Sonke T, Tandler RF, van den Bruel AM, Meijer EM (1991) Anther culture of Lolium perenne and Lolium multiflorum In: Sangwan BS, Sangwan RS (eds) The impact of biotechnology in agriculture. Int Conf, Amiens, France 10–12 July 1989 (in press)Google Scholar
  8. Binh DQ, Heszky LE (1990) Restoration of the regeneration potential of long-term culture in rice (Oryza satira L.) by salt pretreatment. J Plant Physiol 136: 336–340CrossRefGoogle Scholar
  9. Boppenmeier J, Zuchner S, Foroughi-Wehr B (1989) Haploid production from barley yellow dwarf virus resistant clones of Lolium. Plant Breeding 103: 216–220CrossRefGoogle Scholar
  10. Breese EL, Tyler BF (1986) Patterns of variation and the underlying genetic and cytological architecture in grasses with particular reference to Lolium. In: Styles BT (ed) Intraspecific classification of wild and cultivated plants. Clarendon, Oxford, pp 53–69Google Scholar
  11. Chandler SF, Vasil IK (1984) Optimization of plant regeneration from long term embryogenic callus cultures of Pennisetum purpureum grass. J Plant Physiol 117: 147–156PubMedCrossRefGoogle Scholar
  12. Conger BV, Carabia JV, Lowe KW (1978) Comparison of 2,4-D and 2,4,5-T on callus induction and growth in three Gramineae species. Environ Exp Bot 18: 163–168CrossRefGoogle Scholar
  13. Conger BV, Hilenski LL, Lowe KW, Carabia JV (1982) Influence of different auxins at varying concentrations on callus induction and growth from embryo and leaf-tip explants in Gramineae. Environ Exp Bot 22: 39–48CrossRefGoogle Scholar
  14. Creemers-Molenaar J (1991) Regeneration from protoplasts of perennial ryegrass; progress and applications. In: den Nijs APM, Elgersma A (eds) Fodder crops breeding: achievements, novel strategies and biotechnology. Proc 16th Meet Fodder Crops Section of Eucarpia, Wageningen, The Netherlands, 16–22 Nov 1990, pp 123–128Google Scholar
  15. Creemers-Molenaar J, Loeffen JPM, Zaal MACM (1988a) Protoplasts from Lolium perenne L.: procedures for plant regeneration and elettro-fusion. Proc 2nd Int Congr Plant molecular biology, Jerusalem, 13–18 Nov, p 430Google Scholar
  16. Creemers-Molenaar J, Loeffen JPM, Van der Valk P (1988b) The effect of 2,4-dichlorophenoxyacetic acid and donor plant environment on plant regeneration from immature inflorescence-derived callus of Lolium perenne L. and Lolium multiflorum L. Plant Sci 57: 165–172CrossRefGoogle Scholar
  17. Creemers-Molenaar J, Van der Valk P, Loeffen JPM, Zaal MACM (1989) Plant regeneration from suspension cultures and protoplasts of Lolium perenne L. Plant Sci 63: 167–176CrossRefGoogle Scholar
  18. Creemers-Molenaar J, Van Oort Y (1990) Antioxidants influence plating efficiency and microcallus growth of protoplasts in Lolium perenne L. In: Nijkamp HJJ, van der Plas LHW, van Aartrijk J (eds) Progress in plant cellular and molecular biology. Proc 7th Int Congr on Plant tissue and cell culture. Kluwer, Amsterdam, pp 44–49CrossRefGoogle Scholar
  19. Creemers-Molenaar J, van Eeuwyk FA, Krens FA (1992A) Culture optimization for perennial ryegrass protoplasts. J Plant Physiol 139: 303–308Google Scholar
  20. Creemers-Molenaar J, Hall RD, Krens FA (1992B) Asymmetric protoplast fusion aimed at intraspecific transfer of cytoplasmic male sterility (CMS) in Lolium perenne L. Theor. Appl.Google Scholar
  21. Genet. (In Press) Dale PJ (1975) Meristem tip culture in Lolium multiflorum. J Exp Bot 26: 731–736CrossRefGoogle Scholar
  22. Dale PJ (1977a) Meristem tip culture in Lolium, Festuca, Phleum and Dactylis. Plant Sci Lett 9: 333–338 Dale PJ (1977b) The elimination of ryegrass mosaic virus from Lolium multiflorum by meristem tip culture. Ann Appl Biol 85: 93–96CrossRefGoogle Scholar
  23. Dale PJ (1980a) A method for in vitro storage of Lolium multiflorum Lam. Ann Bot 45: 497–502Google Scholar
  24. Dale PJ (19806) Embryoids from cultured immature embryos of Lolium multifiorum Z Pflanzenphysiol 100: 73–77Google Scholar
  25. Dale PJ, Dalton SJ (1983) Immature inflorescence culture in Lolium, Festuca, Phleum and Dactylis. Z Pflanzenphysiol I11: 39–45Google Scholar
  26. Dale PJ, Thomas E, Brettell RIS, Wernicke W (1981) Embryogenesis from cultured immature inflorescences and nodes of Lolium multiflorum. Plant Cell Tissue Organ Cult 1: 47–55CrossRefGoogle Scholar
  27. Dalton SJ (1988a) Plant regeneration from cell suspension protoplasts of Festuca arundinacea Schreb. (tall fescue) and Lolium perenne L. (perennial ryegrass). J Plant Physiol 132: 170–175CrossRefGoogle Scholar
  28. Dalton SJ (1988b) Plant regeneration from cell suspension protoplasts of Festuca arundinacea Schreb., Lolium perenne L. and L. multiflorum Lam. Plant Cell Tissue Organ Cult 12: 137–140CrossRefGoogle Scholar
  29. Datta SK, Peterhans A, Datta K, Potrykus I (1990) Genetically engineered indica-rice recovered from protoplasts. Bio/Technology 8: 736–740CrossRefGoogle Scholar
  30. Day A, Ellis THN (1984) Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplasts. Cell 39: 359–368PubMedCrossRefGoogle Scholar
  31. Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Genet 9: 671–678CrossRefGoogle Scholar
  32. Dudits D, Nemet G, Haydu Z (1975) Study of callus growth and organ formation in wheat (Triticum aestivum) tissue cultures. Can J Bot 53: 957–963CrossRefGoogle Scholar
  33. Fitch MMM, Moore PH (1990) Comparison of 2,4-D and picloram for selection of long-term totipotent green callus cultures of sugarcane. Plant Cell Tissue Organ Cult 20: 157–163Google Scholar
  34. Frakes RV (1973) The ryegrasses. In: Heath ME, Metcalfe DS, Barnes RF (eds) Forages, 3rd edn. Iowa State Univ Press, Ames, Iowa, pp 307–313Google Scholar
  35. Frandsen HN, Frandsen KJ (1948) Polycross-metoden. Nord Jordbruksforsk 7–8: 239–261Google Scholar
  36. Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33: 130–137PubMedCrossRefGoogle Scholar
  37. Glimelius K, Fahlesson J, Landgren M, Sjodin C, Sundberg E (1991) Gene transfer via somatic hybridization in plants. TibTech 9: 24–30CrossRefGoogle Scholar
  38. Gray DJ, Conger BV, Hanning GE (1984) Somatic embryogenesis in suspension and suspension-derived callus cultures_of Dactylis glomerata. Protoplasma 122: 196–202CrossRefGoogle Scholar
  39. Halberg N, Olesen A, Tuvesson IKD, Andersen SB (1990) Genotypes of perennial ryegrass (Lolium perenne L.) with high anther-culture response through hybridization. Plant Breeding 105: 89–94CrossRefGoogle Scholar
  40. Hanauer AR, Miranda JB (1981) Selection: theory. In: Hanauer AR, Miranda JB (eds) Quantitative genetics in maize breeding. Iowa State Univ Press, Ames, Iowa, pp 159–204Google Scholar
  41. Harris R, Wright M, Byrne M, Varnum J, Brightwell B, Schubert K (1988) Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivum L.). Plant Cell Rep 7: 337–340CrossRefGoogle Scholar
  42. Hayward MD, Abdullah IB (1985) Selection and stability of synthetic varieties of Lolium perenne L. The selected character and its expression over generations of multiplication. Theor Appl Genet 70: 48–51Google Scholar
  43. Hayward MD, Olesen A, Due IK, Jenkins R, Morris P (1990) Segregation of isozyme marker loci amongst androgenetic plants of Lolium perenne L. Plant Breeding 104: 68–71CrossRefGoogle Scholar
  44. Ho W, Vasil IK (1983) Somatic embryogenesis in sugarcane (Saccharum officinarum L.): growth and plant regeneration from embryogenic cell suspension cultures. Ann Bot 51: 719–726Google Scholar
  45. Jackson JA, Dale PJ (1988) Callus induction, plant regeneration and an assessment of cytological variation in regenerated plants of Lolium multifiorum L. J Plant Physiol 132: 351–355CrossRefGoogle Scholar
  46. Jackson JA, Dalton SJ, Dale PJ (1986) Plant regeneration from root callus in the forage grass Lolium multifiorum. In: Withers LA, Alderson PJ (eds) Plant tissue culture and its agricultural applications. Butterworths, London, pp 85–89Google Scholar
  47. Joarder 01, Joarder NH, Dale PJ (1986) In vitro response of leaf tissues from Lolium multifiorum - a comparison with leaf segment position, leaf age and in vivo mitotic activity. Theor Appl Genet 73: 286–291CrossRefGoogle Scholar
  48. Jones MGK, Dale PJ (1982) Reproducible regeneration of callus from suspension culture protoplasts of the grass Lolium multifiorum. Z Pflanzenphysiol 105: 267–274Google Scholar
  49. Jonsson HA, Nilsson C (1986) Plant breeding for resistance to snow moulds in grasses. Nord Jordbruksforsk 68: 596Google Scholar
  50. Kamo KK, Hodges TK (1986) Establishment and characterization of long-term embryogenic maize callus and cell suspension cultures. Plant Sci 45: 111–117CrossRefGoogle Scholar
  51. Kasperbauer MJ, Buckner RC, Bush LP (1979) Tissue culture of annual ryegrass x tall fescue F, hybrids: callus establishment and plant regeneration. Crop Sci 19: 457–460CrossRefGoogle Scholar
  52. Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206: 408–413CrossRefGoogle Scholar
  53. Kyozuka J, Otoo E, Shimamoto K (1988) Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet 76: 887–890CrossRefGoogle Scholar
  54. Lee L, Schroll RE, Grimes HD, Hodges TK (1989) Plant regeneration from indica rice (Oryza sativa L.) protoplasts. Planta 178: 325–333CrossRefGoogle Scholar
  55. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127CrossRefGoogle Scholar
  56. Lo PF, Chen CH, Ross JG (1980) Vegetative propagation of temperate forage grasses through callus culture. Crop Sci 20: 363–367CrossRefGoogle Scholar
  57. Lu C, Vasil V, Vasil IK (1981) Isolation and culture of protoplasts of Panicum maximum Jacq. (guinea grass): somatic embryogenesis and plantlet formation. Z Pflanzenphysiol 104: 311–318Google Scholar
  58. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  59. Niizeki M (1977) Haploid, polyploid and aneuploid plants from cultured anthers and calluses in species of Nicotiana and forage crops. J Fac Agric 58: 343–466Google Scholar
  60. Nitzsche W, Wenzel G (1977) Haploids in plant breeding. In: Fortschritte der Pflanzenzüchtung 8. Advances in plant breeding. Parey, Berlin, pp 46–48Google Scholar
  61. Olesen A, Andersen SB, Due IK (1988) Anther culture in perennial ryegrass (Lolium perenne L.). Plant Breeding 101: 60–65CrossRefGoogle Scholar
  62. Orshinsky BR, Mcgregor LJ, Johnson GIE, Hucl P, Kartha K (1990) Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose. Plant Cell Rep 9: 365–369CrossRefGoogle Scholar
  63. Pagniez M, Demarly Y (1979) Obtention d’individus androgenetiques par culture in vitro d’antheres de Ray-grass d’Italie (Lolium multiflorum Lam.). Ann Amelior Plantes 29: 631–637Google Scholar
  64. Plumb RT (1988) The effects of pests and diseases on grasses. In: Jones MB, Lazenby A (eds) The grass crop. Chapman and Hall, London, pp 277–309CrossRefGoogle Scholar
  65. Potrykus 1, Saul MW, Petruska W, Paszkowski J, Shillito RD (1985) Direct gene transfer to cells of a gramineous monocot. Molec Gen Genet 199: 183–188CrossRefGoogle Scholar
  66. Potrykus I (1990) Gene transfer to cereals: an assessment. Bio/Technology 9: 535–542CrossRefGoogle Scholar
  67. Prioli LM, Sondahl MR (1989) Plant regeneration and recovery of fertile plants from protoplasts of maize (Zea mays L.). Bio/Technology 7: 589–594CrossRefGoogle Scholar
  68. Rajoelina SR, Alibert G, Planchon C (1990) Continuous plant regeneration from established embryo-genic cell suspension cultures of Italian ryegrass and tall fescue. Plant Breeding 104: 265–271CrossRefGoogle Scholar
  69. Rhodes CA, Lowe KS, Ruby KL (1988) Plant regeneration from protoplasts isolated from embryo-genic maize cell cultures. Bio/Technology 6: 56–60CrossRefGoogle Scholar
  70. Rose JB, Dunwell JM, Sunderland N (1987) Anther culture of Lolium temulentum, Festuca pratensis and Lolium x Festuca hybrids. I. Influence of pretreatment, culture medium and culture incubation conditions on callus production and differentiation. Ann Bot 60: 191–201Google Scholar
  71. Schaepman H (1952) Application of the polycross test to grass breeding. Euphytica 1: 105–111 Schmidt F (1991) Entwicklung eines Gewebekultursystems bei Lolium perenne (L.) als Voraussetzung zur in vitro- Resistenzselektion. PhD Thesis, Universität HohenheimGoogle Scholar
  72. Schmidt F, Posselt UK (1990) Reproducible regeneration of callus cultures derived from mature embryos in Lolium perenne. In: den Nijs APM, Elgersma A (eds) Fodder crops breeding: achievements, novel strategies and biotechnology. Proc 16th Meet Fodder Crops Section of Eucarpia, Wageningen, The Netherlands, 16–22 Nov 1990, pp 205–206Google Scholar
  73. Shillito RD, Carswell GK, Johnson CM, DiMaio JJ, Harms CT (1989) Regeneration of fertile plants from protoplasts of elite inbred maize. Bio/Technology 7: 581–587CrossRefGoogle Scholar
  74. Skene KGM, Barlass M (1983) Regeneration of plants from callus cultures of Lolium riyidum. Z Pflanzenzücht 90: 130–135Google Scholar
  75. Stanis VA, Butenko RG (1984) Developing viable haploid plants in anther culture of ryegrass. Dokly Biol Sci 275: 249–251Google Scholar
  76. Strickland SG, Nichol JW, McCall CM, Stuart DA (1987) Effect of carbohydrate source on alfalfa somatic embryogenesis. Plant Science 48: 113–121CrossRefGoogle Scholar
  77. Takamizo T, Suginobo K, Ohsugi R (1990) Plant regeneration from suspension culture derived protoplasts of tall fescue (Festuca arundinacea Schreb.) of a single genotype. Plant Science 72: 125–131CrossRefGoogle Scholar
  78. Torello WA, Symington AG (1984) Regeneration from perennial ryegrass callus tissue. Hortic Sci 19: 56–57Google Scholar
  79. Torello WA, Mancino L, Troll J (1983) Initiation, growth, and maintenance of callus tissue derived from mature caryopses of perennial ryegrass (Lolium perenne L.). Rasen Turf Gazon 1: 4–7Google Scholar
  80. Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73: 16–19CrossRefGoogle Scholar
  81. Tuvesson IKD, Pedersen S, Andersen SB (1989) Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture. Theor Appl Genet 78: 879–883CrossRefGoogle Scholar
  82. Tyler BF (1987) Description and distribution of natural variation in forage grasses. In: INRA (eds) Proc Eucarpia fodder crops section meeting, Lusignan, pp 13–22Google Scholar
  83. Utz HF, Oettler G (1978) Performance of inbred lines and their top crosses in perennial ryegrass (Lolium perenne L). Z Pflanzenzücht 80: 223–229Google Scholar
  84. Van der Valk P, Zaal MACM, Creemers-Molenaar J (1988) Regeneration of albino plantlets from suspension culture derived protoplasts of Poa pratensis L. ( Kentucky bluegrass ). Euphytica Suppl: 169–176Google Scholar
  85. Van der Valk P, Zaal MACM, Creemers-Molenaar J (1989) Callus induction and plant regeneration from immature inflorescences and mature seeds of Poa pratensis L. ( Kentucky bluegrass ). Plant Cell Rep 7: 644–647Google Scholar
  86. Vasil IK (1987) Developing cell and tissue culture systems for the improvement of cereal and grass crops. J Plant Physiol 128: 193–218CrossRefGoogle Scholar
  87. Vasil IK (1988) Progress in the regeneration and the genetic manipulation of cereal crops. Bio/Technology 6: 397–402CrossRefGoogle Scholar
  88. Vasil IK (1990) The realities and challenges of plant biotechnology. Bio/Technology 8: 296–301CrossRefGoogle Scholar
  89. Vasil V, Vasil 1K (1986) Plant regeneration from friable embryogenic callus and cell suspension cultures of Zea mays L. J Plant Physiol 124: 399–408Google Scholar
  90. Vasil V, Redway F, Vasil IK (1990) Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio/Technology 8: 429–434CrossRefGoogle Scholar
  91. Wang C, Sun C, Chu C, Wu S (1978) Studies on the albino pollen plantlets in rice. In: Proc Symp on Plant tissue culture, Pittman, Peking, pp 149–160Google Scholar
  92. Wang D, Miller PD, Sondahl MR (1989) Plant regeneration from protoplasts of indica type rice and CMS rice. Plant Cell Rep 8: 329–332CrossRefGoogle Scholar
  93. Wang WC, Nguyen HT (1990) A novel approach for efficient plant regeneration from long-term suspension culture of wheat. Plant Cell Rep 8: 639–642CrossRefGoogle Scholar
  94. Wang X, Hu Han (1984) The effect of potato II medium for Triticale anther culture. Plant Sci Lett 36: 237–239CrossRefGoogle Scholar
  95. Wit F (1959) Chromosome doubling and the improvement of grasses. Agraria 11: 97–115Google Scholar
  96. Yamada Y, Zhi-Qi Y, Ding-Tai T (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 5: 85–88CrossRefGoogle Scholar
  97. Yasuda T, Miyano S, Yamamoto Y, Uchida N, Yamaguchi T (1990) Analogues of phenoxyacetic acid and the regeneration of calluses from seeds of indica rice. Plant Cell Physiol 31 (6): 763–766Google Scholar
  98. Zaghmout OMF, Torello WA (1989) Somatic embryogenesis and plant regeneration from suspension cultures of red fescue. Crop Sci 29: 815–817CrossRefGoogle Scholar
  99. Zaghmout OMF, Torello WA (1990b) Somatic embryogenesis and plant regeneration from embryo-genic suspension cultures of perennial ryegrass. In Vitro Cell Dev Biol 26: 419–424Google Scholar
  100. Zimny J, Lorz H (1986) Plant regeneration and initiation of cell suspensions from root-tip derived callus of Oryza satina L. Plant Cell Rep 5: 89–92CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J. Creemers-Molenaar
    • 1
  • L. J. Beerepoot
    • 1
  1. 1.Barenbrug Holland BVOosterhoutThe Netherlands

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