Status of Isoenzyme Research

  • Marian L. Thorpe
  • Louise H. Duke
Part of the Monographs on Theoretical and Applied Genetics book series (GENETICS, volume 19)


Studies of isoenzymes of Brassica species were initiated in 1967 (Vaughan and Waite 1967 a, b). Initially, the research was concerned mainly with the taxonomy of Brassica species (Vaughan and Waite 1967a, b, 1968; Vaughan and Denford 1968); subsequently, however, the use of isoenzymes as genetic markers for cultivar identification, purity analysis (Chen and Tong 1985; Thorpe et al. 1987; Duke 1988) and for identification of hybrids received greater attention (Wills et al. 1979; Kato and Tokumasu 1979; Thorpe and Beversdorf 1988; Toriyama et al. 1987). Isoenzymes have also been used to study the effectiveness of plant transformation (Mathews et al. 1985) and tissue culture techniques (Cardy 1986; Charne et al. 1988). The association between isoenzymes and agronomic traits has been reported by a few researchers (Thukral et al. 1985). A limited amount of research has been done on the genetics of specific isoenzymes (Arus and Orton 1983; Quiros et al. 1987); results from most isoenzyme research are reported as zymograms. Each of the above-mentioned applications of isoenzyme research to the breeding of oilseed Brassica species will be covered in detail in this chapter.


Cytoplasmic Male Sterile Brassica Napus Somatic Hybrid Brassica Species Microspore Culture 
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© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Marian L. Thorpe
  • Louise H. Duke

There are no affiliations available

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