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Brassica Protoplast Culture and Somatic Hybridization

  • Chapter
Brassica

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 54))

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Abstract

Since the regeneration of the first plants from Brassica protoplasts and the subsequent production of the first B. napus somatic hybrid (Schenck and Röbbelen 1982), somatic hybridization has become an important tool in Bras-sica breeding for the transfer of agronomically important traits. Conventional plant breeding enables the introgression of traits only from close relatives as gene exchange between species is usually restricted by incompatibility barriers. However, the use of genetic engineering (GE) or protoplast fusion can overcome these barriers. GE enables foreign DNA to be transferred directly into the host plant’s genome; therefore, genetic incompatibility is not an issue providing a transformation system is available. However, only a limited number of cloned genes are available for transfer, and often patents protect commercial use of these genes. As well, adverse public perceptions hamper the use of this technology. The advantage of protoplast fusion is that DNA can be introgressed from sexually incompatible species. In addition, several unidentified genes may be transferred at once.

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  1. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand

    M. C. Christey

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  1. M. C. Christey
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  1. Plant Genetic Engineering Laboratory, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore, Republic of Singapore

    Eng-Chong Pua

  2. Department of Botany, University of British Columbia, V6T 1 Z4, Vancouver, British Columbia, Canada

    Carl J. Douglas

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© 2004 Springer-Verlag Berlin Heidelberg

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Christey, M.C. (2004). Brassica Protoplast Culture and Somatic Hybridization. In: Pua, EC., Douglas, C.J. (eds) Brassica. Biotechnology in Agriculture and Forestry, vol 54. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06164-0_8

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  • DOI: https://doi.org/10.1007/978-3-662-06164-0_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05783-0

  • Online ISBN: 978-3-662-06164-0

  • eBook Packages: Springer Book Archive

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