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Regeneration of Plants via Somatic Embryogenesis

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Brassica

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 54))

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Abstract

Somatic embryogenesis, first reported in tissue cultures of carrot (Steward et al.1958), has since been demonstrated in a vast number of species (Ammirato 1983). It is the process in which a bipolar structure with no vascular connection to the parental tissue is formed from somatic cells (i.e. cells other than those produced from gametic fusion), via passage through a series of stages characteristic for zygotic embryo development (Sharp et al. 1980). Somatic embryogenesis demonstrates the totipotency of plant cells (Raghavan 1997), and is of considerable importance theoretically and practically because it can be used to combine efficient cloning of desired genotypes with genetic modification (Sharp et al. 1980). Plant regeneration from callus cultures, although used for improvement of economically important crops, is often associated with genetic and cytological variations (Larkin and Scowcraft 1981), which are not always desirable. Thus somatic embryogenesis has better applicability in the improvement of crops, as plants regenerated from direct somatic embryogenesis are often more uniform than plants obtained via callus tissues (Maheswaran and Williams 1984).

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Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Republic of Singapore

    C. S. Loh

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  1. C. S. Loh
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  2. W. Shu
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  3. W. L. Koh
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Editors and Affiliations

  1. Plant Genetic Engineering Laboratory, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore, Republic of Singapore

    Eng-Chong Pua

  2. Department of Botany, University of British Columbia, V6T 1 Z4, Vancouver, British Columbia, Canada

    Carl J. Douglas

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Loh, C.S., Shu, W., Koh, W.L. (2004). Regeneration of Plants via Somatic Embryogenesis. In: Pua, EC., Douglas, C.J. (eds) Brassica. Biotechnology in Agriculture and Forestry, vol 54. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06164-0_7

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  • DOI: https://doi.org/10.1007/978-3-662-06164-0_7

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