Brassica pp 83-102 | Cite as

Regulation of Plant Morphogenesis in Vitro

  • E. C. Pua
  • H. Gong
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 54)


Genetic engineering is a powerful tool for crop improvement by production of novel transgenic plants with desirable traits (Herbers and Sonnewald 1996; Mazur et al. 1999; Dunwell 2000), and for study of gene function and regulation (Schell 1987; Willmitzer 1988). However, the success of plant genetic engineering has relied mainly on the availability of efficient tissue culture systems, in which plants can be regenerated from cultured cells and tissues at high frequencies, via somatic embryogenesis (SE) and de novo shoot organogenesis. Regeneration systems are essential for transformed cells expressing the introduced transgenes to form plants. Furthermore, the ability to regenerate large numbers of genetically identical plants in culture is an important means of vegetative propagation, which is commonly employed today by commercial nurseries for large-scale production of various ornamental, vegetable, fruit and field crops.


Somatic Embryogenesis Ethylene Production Shoot Regeneration Anther Culture Shoot Organogenesis 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • E. C. Pua
  • H. Gong
    • 1
  1. 1.Plant Genetic Engineering Laboratory, Department of Biological Sciences, Faculty of ScienceNational University of SingaporeSingaporeRepublic of Singapore

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