Skip to main content

Regulation of Plant Morphogenesis in Vitro

  • Chapter
Brassica

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 54))

Abstract

Genetic engineering is a powerful tool for crop improvement by production of novel transgenic plants with desirable traits (Herbers and Sonnewald 1996; Mazur et al. 1999; Dunwell 2000), and for study of gene function and regulation (Schell 1987; Willmitzer 1988). However, the success of plant genetic engineering has relied mainly on the availability of efficient tissue culture systems, in which plants can be regenerated from cultured cells and tissues at high frequencies, via somatic embryogenesis (SE) and de novo shoot organogenesis. Regeneration systems are essential for transformed cells expressing the introduced transgenes to form plants. Furthermore, the ability to regenerate large numbers of genetically identical plants in culture is an important means of vegetative propagation, which is commonly employed today by commercial nurseries for large-scale production of various ornamental, vegetable, fruit and field crops.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Achar PN (2002) A study of factors affecting embryo yields from anther culture of cabbage. Plant Cell Tissue Organ Cult 69: 183–188

    Article  Google Scholar 

  • Allen RD, Webb RP, Schake SA (1997) Use of transgenic plants to study antioxidant defenses. Free Radical Biol Med 23: 473–479

    Article  CAS  Google Scholar 

  • Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR (1999) EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science 284: 2148–2152

    Article  PubMed  CAS  Google Scholar 

  • Banno H, Ikeda Y, Niu QW, Chua NH (2001) Overexpression of Arabidopsis ESR1 induces initiation of shoot regeneration. Plant Cell 13: 2609–2618

    PubMed  CAS  Google Scholar 

  • Beyer EM Jr (1976) A potent inhibitor of ethylene action in plants. Plant Physiol 58:268–271 Biddington NL, Sutherland RA, Robinson HT (1988) Silver nitrate increases embryo production in anther culture of Brussels sprouts. Ann Bot 62: 181–185

    Google Scholar 

  • Bleecker AB, Kende H (2000) Ethylene: a gaseous signal molecule in plants. Annu Rev Cell Dev Biol 16: 1–18

    Article  PubMed  CAS  Google Scholar 

  • Boutilier K, Offringa R, Sharma VK, Kleft H, Quellet T, Zhang L, Hattori J, Liu CM, van Lammeren AAM, Miki BLA, Custers JBM, van Lookeren Campagne MM (2002) Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryogenic growth. Plant Cell 14: 1737–1749

    Article  PubMed  CAS  Google Scholar 

  • Bregoli AM, Scaramagli S, Costa G, Sabatini E, Ziosi V, Biondi S, Torrigiani P (2002) Peach (Prunus persica) fruit ripening: aminoethoxyvinylglycine ( AVG) and exogenous polyamines affect ethylene emission and fruit firmness. Physiol Plant 114: 472–481

    Google Scholar 

  • Burnett L,Arnoldo M, Tarrow S, Huang B (1994) Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp. oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors. Plant Cell Tissue Organ Cult 37: 253–256

    Google Scholar 

  • Cary A, Uttamchandani SJ, Smets R, Van Onckelen HA, Howell SH (2001) Arabidopsis mutants with increased organ regeneration in tissue culture are more competent to respond to hormonal signals. Planta 213: 700–707

    Google Scholar 

  • Catterou M, Dubois F, Smets R, Vaniet S, Kichey T, Van Onckelen H, Sangwan-Norreel BS, Sangwan1RS (2002) hoc: an Arabidopsis mutant overproducing cytokinins and expressing high in vitro organogenic capacity. Plant J 30: 273–287

    Google Scholar 

  • Che P, Gingerich DJ, Lall S, Howell SH (2002) Global and hormone-induced gene expression changes during shoot development in Arabidopsis. Plant Cell 14: 2771–2785

    Article  PubMed  CAS  Google Scholar 

  • Cheng PK, Lakshmanan P, Swarup S (2001) High frequency direct shoot regeneration and continuous production of rapid-cycling Brassica oleracea in vitro. In Vitro Cell Dev Biol-Plant 37: 592–598

    Google Scholar 

  • Cheng W (2002) Molecular characterization and regulation of 1-aminocyclopropane-1-carboxylate synthase in shoot morphogenesis of mustard in vitro. PhD Thesis, National University of Singapore, Singapore

    Google Scholar 

  • Chi GL, Pua EC (1989) Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis ( Chinese cabbage) in vitro. Plant Sci 64: 243–250

    Google Scholar 

  • Chi GL, Barfield DG, Sim GE, Pua EC (1990) Effect of AgNO3 and aminoethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes. Plant Cell Rep 9: 195–198

    Article  CAS  Google Scholar 

  • Chi GL, Pua EC, Goh CJ (1991) Role of ethylene on de novo shoot morphogenesis from cotyledons of Brassica campestris ssp. pekinensis ( Lour) Olsson in vitro. Plant Physiol 96: 178–183

    Google Scholar 

  • Chi GL, Lin WS, Lee JEE, Pua EC (1994) Role of polyamines on de novo shoot morphogenesis from cotyledons of Brassica campestris ssp. pekinensis ( Lour) Olsson in vitro. Plant Cell Rep 13: 323–329

    Google Scholar 

  • Cho HS, Cao J, Ren JP, Earle ED (2001) Control of lepidopteran insect pests in transgenic Chinese cabbage (Brassica rapa ssp. pekinensis) transformed with a synthetic Bacillus thuringiensis cry1C gene. Plant Cell Rep 20: 1–7

    Article  CAS  Google Scholar 

  • Chraibi BKM, Latché A, Roustan JP, Fallot J (1991) Stimulation of shoot regeneration from cotyledons of Helianthus annuus by ethylene inhibitors, silver and cobalt. Plant Cell Rep 10: 204–207

    Google Scholar 

  • Cui KR, Xing GS, Liu XM, Xing GM, Wang YF (1999) Effect of hydrogen peroxide on somatic embryogenesis of Lycium barbarum L. Plant Sci 146: 9–16

    Article  CAS  Google Scholar 

  • Cui K, Li J, Xing G, Li J, Wang L, Wang Y (2002) Effect of hydrogen peroxide on synthesis of proteins during somatic embryogenesis in Lycium barbarum. Plant Cell Tissue Organ Cult 68: 187–193

    Article  CAS  Google Scholar 

  • Daimon Y, Takabe K, Tasaka M (2003) The CUP-SHAPRED COTYLEDON genes promote adventitious shoot formation. Plant Cell Physiol 44: 113–121

    Article  PubMed  CAS  Google Scholar 

  • De Block M, de Brouwer D, Tenning T (1989) Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol 91: 694–701

    Article  PubMed  Google Scholar 

  • De Marco A, Roubelakis-Angelakis KA (1996) The complexity of enzyme control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts. Plant Physiol 110: 137–145

    PubMed  Google Scholar 

  • Dias JS, Martins MG (1999) Effect of silver nitrate on anther culture embryo production of different Brassica oleracea morphotypes. Sci Hortic 82: 299–307

    Article  CAS  Google Scholar 

  • Ding LC, Hu CY, Yeh KW, Wang PJ (1998) Development of insect-resistant transgenic cauliflower plants expressing the trypsin inhibitor gene isolated from local sweet potato. Plant Cell Rep 17: 854–860

    Article  CAS  Google Scholar 

  • Dulius H (1991) Inheritance of the regeneration capacity in the genus Petunia. Euphytica 53: 173–181

    Article  Google Scholar 

  • Dunwell JM (2000) Transgenic approaches to crop improvement. J Exp Bot 51: 487–498

    Article  PubMed  CAS  Google Scholar 

  • Eapen S, George L (1997) Plant regeneration from peduncle segments of oil seed Brassica species: influence of silver nitrate and silver thiosulfate. Plant Cell Tissue Organ Cult 51: 229–232.

    Article  CAS  Google Scholar 

  • Evans PT, Malmberg RL (1989) Do polyamines have roles in plant development? Annu Rev Plant Physiol Plant Mol Biol 40: 235–269

    Article  CAS  Google Scholar 

  • Galston AW, Sawhney RK (1990) Polyamines in plant physiology. Plant Physiol 94: 406–410

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Plant cell cultures. I. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Google Scholar 

  • Glimelius K (1984) High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol Plant 61: 38–44

    Article  CAS  Google Scholar 

  • Grady KL, Bassham JA (1982) 1-Aminocyclopropane-1-carboxylic acid in shoot forming and non-shoot forming tobacco callus cultures. Plant Physiol 70: 919–921

    Google Scholar 

  • Hachey JE, Sharma KK, Moloney MM (1991) Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro. Plant Cell Rep 9: 549–554

    Article  CAS  Google Scholar 

  • Hays DB, Reid DM, Yeung EC, Pharis RP (2000) Role of ethylene in cotyledon development of microspore-derived embryos of Brassica napus. J Exp Bot 51: 1851–1859

    Article  PubMed  CAS  Google Scholar 

  • Heby O, Persson L (1990) Molecular genetics of polyamine synthesis in eukaryotic cells. Trends Biochem Sci 15: 153–158

    Article  PubMed  CAS  Google Scholar 

  • Hecht V, Vielle-Calzada JP, Hartog MV, Schmidt EDI, Boutilier K, Grossniklaus U, de Vries SC (2001) The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenesis competence in culture. Plant Physiol 127: 803–816

    Article  PubMed  CAS  Google Scholar 

  • Herbers K, Sonnewald U (1996) Manipulating metabolic partitioning in transgenic plants. Trends Biotechnol 14: 198–205

    Article  PubMed  CAS  Google Scholar 

  • Hirayama T, Kieber JJ, Hirayama N, Kogan M, Guzman P, Nourizadeh S, Alonso JM, Ecker JR (1999) RESPONSIVE-TO-ANTAGONIST1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis. Cell 97: 383–393

    Article  PubMed  CAS  Google Scholar 

  • Hua J, Sakai H, Nourizadeh S, Chen QG, Bleecker AB, Ecker JR, Meyerowitz EM (1998) EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis. Plant Cell 10: 1321–1332

    PubMed  CAS  Google Scholar 

  • Huxter TJ, Thorpe TA, Reid DM (1981) Shoot initiation in light-and dark-grown tobacco callus: the role of ethylene. Physiol Plant 53: 319–326

    Article  CAS  Google Scholar 

  • Jain PK, Chowdbury JB, Sharma DR, Friedt W (1988) Genotypic and media effects on plant regeneration from cotyledonary explant cultures of some Brassica species. Plant Cell Tissue Organ Cult 14: 197–206

    Article  Google Scholar 

  • Jourdan PS, Earle ED (1989) Genotypic variability in the frequency of plant regeneration from leaf protoplasts of four Brassica spp. and Raphanus sativus. J Am Soc Hortic Sci 114: 343–349

    Google Scholar 

  • Khan MR, Rashid H,Ansar M, Chaudry Z (2003) High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus). Plant Cell Tissue Organ Cult 75: 223–231

    Article  Google Scholar 

  • Kakimoto T (1996) CK1, a histidine kinase homolog implicated in cytokinin signal transduction. Science 274: 982–985

    Article  PubMed  CAS  Google Scholar 

  • Keller WA, Armstrong KC (1977) Embryogenesis and plant regeneration in Brassica napus through anther cultures. Can J Bot 55: 1383–1388

    Article  Google Scholar 

  • Keller WA, Rajhathy T, Lacapra J (1975) In vitro production of plants from pollen in Brassica campestris. Can J Genet Cytol 17: 655–666

    CAS  Google Scholar 

  • Koornneef M, Bade J, Hanhart C, Horsman K, Schel J, Soppe W, Verkerk R, Zabel P (1993) Characterizationand mapping of a gene controlling shoot regeneration in tomato. Plant J 3: 131–141

    Article  CAS  Google Scholar 

  • Kubo M, Kakimoto T (2000) The CYTOKININ-HYPERSENSITIVE genes of Arabidopsis negatively regulate the cytokinin signaling pathway for cell proliferation and chloroplast development. Plant J 23: 385–394

    Article  PubMed  CAS  Google Scholar 

  • Kuvshinov V, Koivu K, Kanerva A, Pehu E (1999) Agrobacterium tumefaciens-mediated transformation of greenhouse-grown Brassica rapa ssp. oleifera. Plant Cell Rep 18: 773–777

    Google Scholar 

  • Kvaalen H (1994) Ethylene synthesis and growth in embryogenic tissues of Norway spruce. Effect of oxygen, 1-aminocyclopropane-1-carboxylic acid, benzyladenine and 2,4-dichlorophenoxyacetic acid. Physiol Plant 76: 521–526

    Google Scholar 

  • LaRue TAG, Gamborg OL (1971) Ethylene production by plant cell cultures: variation in production during growing cycle and in different plant species. Plant Physiol 48: 394–398

    Article  PubMed  CAS  Google Scholar 

  • Lentini Z, Mussell H, Mutschler MA, Earle ED (1988) Ethylene generation and reversal of ethylene effects during development in vitro of rapid-cycling Brassica campestris L. Plant Sci 54: 75–81

    Article  CAS  Google Scholar 

  • Lim CC, Liu JZ, Pua EC (2002) Characterization of S-adenosylmethionine synthetase genes and its expression is associated with ethylene synthesis in mustard (Brassica juncea [L.] Czern & Coss). Physiol Plant 116: 522–530

    Article  CAS  Google Scholar 

  • Lim SH (2002) ACC oxidase expression and the interactive role of ethylene and polyamines in shoot morphogenesis of mustard in vitro. MSc Thesis, National University of Singapore, Singapore

    Google Scholar 

  • Luo JP, Jiang ST, Pan LJ (2001) Enhanced somatic embryogenesis by salicylic acid of Astragalus adsurgens Pall: relationship with H2O2 production and H2O2-metabolizing enzyme activities. Plant Sci 161: 125–132

    Article  CAS  Google Scholar 

  • Mackenzie IA, Street HE (1970) Studies on the growth in culture of plant cells. VIII. The production of ethylene by suspension cultures of Acer pseudoplatanus. J Exp Bot 21: 824–834

    Article  CAS  Google Scholar 

  • Malik CR, Rangaswamy NS, Shivanna KR (2001) Induction of microspore embryos in a CMS line in Brassica juncea and formation of the androgenic plantlets. Euphytica 120: 195–203

    Article  CAS  Google Scholar 

  • Mazur B, Krebbers E, Tingey S (1999) Gene discovery and product development for grain quality traits. Science 285: 372–379

    Article  PubMed  CAS  Google Scholar 

  • Mensuari-Sodi A, Panizza M, Tognoni F (1992) Quantitation of ethylene losses in different container-sealed systems and comparison of biotic and abiotic contributions to ethylene. Physiol Plant 84: 472–476

    Article  Google Scholar 

  • Mo H, Pua EC (2002) Up-regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea) in response to stress. Physiol Plant 114: 439–449

    Article  PubMed  CAS  Google Scholar 

  • Mukhopadhyay A, Arumugam N, Nandakumar PBA, Pradhan AK, Gupta V, Pental D (1992) Agrobacterium-mediated genetic transformation of oilseed Brassica campestris: transformation frequency is strongly influenced by the mode of shoot regeneration. Plant Cell Rep 11: 506–513

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays in tobacco tissue culture. Physiol Plant 15: 473–493

    Article  CAS  Google Scholar 

  • Nadolska-Orczyk A, Malepszy S (1989) In vitro culture of Cucumis sativus L. 7. Genes controlling plant regeneration. Theor Appl Genet 78: 836–840

    Google Scholar 

  • Narasimhulu SB, Chopra VL (1988) Species specific shoot regeneration response of cotyledonary explants of Brassicas. Plant Cell Rep 7: 104–106

    Article  Google Scholar 

  • Narasimhulu SB, Prakash S, Chopra VL (1988) Comparative shoot regeneration responses of diploid brassicas and their synthetic amphidiploid products. Plant Cell Rep 7: 525–527

    Article  Google Scholar 

  • Ockendon DJ,McClenaghan R (1993) Effect of silver nitrate and 2,4-D on anther culture of Brussels sprouts (Brassica oleracea var. gemmifera). Plant Cell Tissue Organ Cult 32: 41–46

    Article  Google Scholar 

  • Ogas J, Cheng JC, Sung ZR, Somerville C (1997) Cellular differentiation regulated by gibberellin in the Arabidopsis thaliana pickle mutant. Science 277: 91–94

    Article  PubMed  CAS  Google Scholar 

  • Ono Y, Takahata Y (2000) Genetic analysis of shoot regeneration from cotyledonary explants in Brassica napus. Theor Appl Genet 100: 895–898

    Article  Google Scholar 

  • Ono Y, Takahata Y, Kaizuma N (1994) Effect of genotype on shoot regeneration from cotyledonary explants of rapeseed (Brassica napus L.). Plant Cell Rep 14: 13–17

    Article  CAS  Google Scholar 

  • Palmer EE (1992) Enhanced shoot regeneration from Brassica campestris by silver nitrate. Plant Cell Rep 11: 541–545

    Article  CAS  Google Scholar 

  • Penarrubia L, Aguilar M, Margissian L, Fischer RL (1992) An antisense gene stimulates ethylene hormone production during tomato fruit ripening. Plant Cell 4: 681–687

    PubMed  CAS  Google Scholar 

  • Pental D, Pradhan AK, Sodhi YS, Mukhopadhyay A (1993) Variation amongst Brassica juncea cultivars for regeneration from hypocotyl explants and optimization for Agrobacteriummediated genetic transformation. Plant Cell Rep 12: 462–467

    Article  CAS  Google Scholar 

  • Perl A, Aviv D, Galun E (1988) Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improved yield, plating efficiency and transient expression of an alien gene. Plant Cell Rep 7: 403–406

    CAS  Google Scholar 

  • Pua EC (1993) Cellular and molecular aspects of ethylene on plant morphogenesis of recalcitrant Brassica species in vitro. Bot Bull Acad Sin 34: 191–209

    CAS  Google Scholar 

  • Pua EC (1994) Regeneration of plants from protoplasts of Brassica juncea (L.) Czern & Coss (brown mustard). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 29. Plant protoplasts and genetic engineering V. Springer, Berlin Heidelberg New York, pp 38–51

    Google Scholar 

  • Pua EC (1999) Morphogenesis in cell and tissue cultures: role of ethylene and polyamines. In: Soh WY, Bhojwani SS (eds) Morphogenesis in plant tissue cultures. Kluwer, Dordrecht, pp 255–303

    Google Scholar 

  • Pua EC, Chi GL (1993) De novo shoot morphogenesis and plant growth of mustard (Brassica juncea) in vitro in relation to ethylene. Physiol Plant 88: 467–474

    Article  CAS  Google Scholar 

  • Pua EC, Lee JEE (1995) Enhanced de novo shoot morphogenesis in vitro by expression of anti-sense 1-aminocyclopropane-1-carboxylate oxidase gene in transgenic mustard plants. Planta 196: 69–76

    Article  CAS  Google Scholar 

  • Pua EC, Teo SH, Loh CS (1996) Interactive role of ethylene and polyamines on shoot regenerability of Chinese kale (Brassica alboglabra Bailey) in vitro. J Plant Physiol 149: 138–148

    Article  CAS  Google Scholar 

  • Pua EC, Deng X, Koh ATC (1999) Genotypic variability of de novo shoot morphogenesis of Brassica oleracea in vitro in response to ethylene inhibitors and putrescine. J Plant Physiol 155: 598–605

    Article  CAS  Google Scholar 

  • Radke SE, Turner JC, Facciotti D (1992) Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens. Plant Cell Rep 11: 499–505

    Article  Google Scholar 

  • Rethmeier NOM, Jansen CE, Snel EAM, Nijkamp HJJ, Hille J (1991) Improvement of regeneration of Lycopersicon pennillii by decreasing ethylene production. Plant Cell Rep 9: 539–543

    Article  CAS  Google Scholar 

  • Roberts DR, Walker MA, Thompson JE, Dumbroff EB (1983) The effects of inhibitors of polyamine and ethylene biosynthesis on senescence, ethylene production and polyamine levels in cut carnation. Plant Cell Physiol 25: 315–322

    Google Scholar 

  • Rodriguez FI, Esch JJ, Hall AE, Binder BM, Schaller E, Bleecker AB (1999) A copper cofactor for the ethylene receptor ETR1 from Arabidopsis. Science 283: 996–998

    Article  PubMed  CAS  Google Scholar 

  • Rupp HM, Frank M, Werner T, Strnad M, Schmülling T (1999) Increased steady state mRNA levels of the STM and KNAT1 homeobox genes in cytokinin overproducing Arabidopsis thaliana indicate a role for cytokinins in the shoot apical meristem. Plant J 18: 557–563

    Article  PubMed  CAS  Google Scholar 

  • Sagi M, Fluhr R (2001) Superoxide production by plant homologues of the gp91phox NADPH oxidase. Modulation of activity by calcium and by tobacco mosaic virus infection. Plant Physiol 126: 1281–1290

    Google Scholar 

  • Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol 101: 7–12

    PubMed  CAS  Google Scholar 

  • Schell J (1987) Transgenic plants as tools to study the molecular organization of plant genes. Science 237: 1176–1182

    Article  Google Scholar 

  • Schmidt EDL, Guzzo F, Toonen MAJ, de Vries SC (1997) A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124: 2049–2062

    PubMed  CAS  Google Scholar 

  • Scholten HJ, Pierik RLM (1998) Agar as a gelling agent: chemical and physical analysis. Plant Cell Rep 17: 230–235

    Article  CAS  Google Scholar 

  • Sethi U, Basu A, Guha-Mukherjee S (1990) Control of cell proliferation and differentiation by modulators of ethylene biosynthesis and action in Brassica hypocotyls explants. Plant Sci 69: 225–229

    Article  CAS  Google Scholar 

  • Sharma KK, Bhojwani SS (1990) Histological aspects on in vitro root and shoot differentiation from cotyledon explants of Brassica juncea ( L.) Czern. Plant Sci 69: 207–214

    Google Scholar 

  • Shoeb F, Yadav JS, Bajaj S, Rajam MV (2001) Polyamines as biomarkers as plant regeneration capacity: improvement of plant regeneration by modulation of polyamine metabolism in different genotypes of indica rice. Plant Sci 160: 1229–1235

    Article  PubMed  CAS  Google Scholar 

  • Stewart ER, Freebaire HT (1967) Ethylene production in tobacco pith cultures. Plant Physiol 42: S30

    Article  Google Scholar 

  • Subrahmanyam D, Rathore VS (1992) Influence of ethylene on 14 CO2 assimilation and partitioning in Indian mustard. Plant Physiol Biochem 30: 81–86

    CAS  Google Scholar 

  • Taguchi-Shiobara F, Komatsuda T, Oka S (1997) Comparison of two indices for evaluating regeneration ability in rice (Oryza sativa L.) through a diallel analysis. Theor Appl Genet 94: 378–382

    Article  Google Scholar 

  • Takada S, Hibara K, Ishida T, Tasaka M (2001) The CUP-SHAPED COTYLEDONS gene of Arabidopsis regulates shoot apical meristem formation. Development 128: 1127–1135

    PubMed  CAS  Google Scholar 

  • Teo WL, Kumar P, Goh CJ, Swarup S (2001) The expression of Brostm, a KNOTTED1-like gene, marks the cell type and timing of in vitro shoot induction in Brassica oleracea. Plant Mol Biol 46: 567–580

    Article  PubMed  CAS  Google Scholar 

  • Tomes DT, Smith OS (1985) The effect of parental genotype on initiation of embryogenic callus from elite maize (Zea mays L.) germplasm. Theor Appl Genet 70: 505–509

    Article  Google Scholar 

  • UN (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and its peculiar mode of fertilization. Jpn J Bot 7: 389–452

    Google Scholar 

  • Walden R, Cordeiro A, Tiburcio AF (1997) Polyamines: small molecules triggering pathways in plant growth and development. Plant Physiol 113: 1009–1013

    Article  PubMed  CAS  Google Scholar 

  • Wang KLC, Li H, Ecker JR (2002) Ethylene biosynthesis and signaling networks. Plant Cell S131- S151

    Google Scholar 

  • Williams J, Pink DAC, Biddington NL (1990) Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera. Plant Cell Tissue Organ Cult 21: 61–66

    Article  CAS  Google Scholar 

  • Willmitzer L (1988) The use of transgenic plants to study plant gene expression. Trends Genet 4: 13–18

    Article  PubMed  CAS  Google Scholar 

  • Xiang Y, Wong WKR, Ma MC, Wong RSC (2000) Agrobacterium-mediated transformation of Bras-sica campestris ssp. parachinensis with synthetic Bacillus thuringiensis cry1Ab and cry1Ac genes. Plant Cell Rep 19: 251–256

    Google Scholar 

  • Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35: 155–189

    Article  CAS  Google Scholar 

  • Zhang FL, Takahata Y (2001) Inheritance of microspore embryogenic ability in Brassica crops. Theor Appl Genet 103: 254–258

    Article  CAS  Google Scholar 

  • Zhang FL, Takahata Y, Xu JB (1998) Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep 17: 780–786

    Article  CAS  Google Scholar 

  • Zhang FL, Takahata Y, Watanabe M, Xu JB (2000) Agrobacterium-mediated transformation of cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep 19: 569–575

    Google Scholar 

  • Zhang S, Wong L, Meng L, Lemaux PG (2002) Similarity of expression patterns of knotted1 and ZmLEC1 during somatic and zygotic embryogenesis in maize (Zea mays L.). Planta 215: 191–194

    Article  PubMed  CAS  Google Scholar 

  • Zobayed SMA, Armstrong J, Armstrong W (1999) Evaluation of a closed system, diffusive and humidity-induced convective throughflow ventilation on the growth and physiology of cauliflower in vitro. Plant Cell Tissue Organ Cult 59: 113–123

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Pua, E.C., Gong, H. (2004). Regulation of Plant Morphogenesis in Vitro. In: Pua, EC., Douglas, C.J. (eds) Brassica. Biotechnology in Agriculture and Forestry, vol 54. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06164-0_6

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-06164-0_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05783-0

  • Online ISBN: 978-3-662-06164-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics