Brassica pp 31-42 | Cite as

Genome Mapping and Analysis

  • C. F. Quiros
  • A. H. Paterson
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 54)


The present chapter is aimed to build on, and update, an earlier comprehensive review (Quiros 2001) by providing the latest information on gene mapping, its analysis and application in Brassica crops.


Doubled Haploid Chinese Cabbage Inter Simple Sequence Repeat Oilseed Rape Brassica Oleracea 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Acarkan A, Koch M, Rossberg M, Schmidt R (1998) Comparative genome analysis in cruciferous plants. Plant Animal Genome VI, Abstract no P213, p 131Google Scholar
  2. Ajisaka H, Kuginuki Y, Yui S, Enomoto S, Hirai M (2001) Identification and mapping of a quantitative trait locus controlling extreme late bolting in Chinese cabbage (Brassica rapa L. ssp. pekinensis syn. campestris L.) using bulked segregant analysis–a QTL controlling extreme late bolting in Chinese cabbage. Euphytica 118: 75–81CrossRefGoogle Scholar
  3. Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796–815Google Scholar
  4. Arus P, Orton T (1983) Inheritance and linkage relationships of isozyme loci in Brassica oleracea. J Hered 74: 405–412Google Scholar
  5. Babula D, Kaczmarck M, Barakat A, Delseny M, Quiros CF, Sadowski J (2003) Chromosomal mapping of B. oleracea based on A. thaliana ESTs: complexity of comparative map. Mol Gen Genet 268: 656–665Google Scholar
  6. Blanc G, Barakat A, Guyot R, Cooke R, Delseny M (2000) Extensive duplication and reshuffling in the Arabidopsis genome. Plant Cell 12: 1523–1540Google Scholar
  7. Bornet B, Muller C, Paulus F, Branchard M (2002) Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using tri-and tetra-nucleotide primers from DNA of cauliflower (Brassica oleracea var. botrytis L.). Genome 45: 890–896PubMedCrossRefGoogle Scholar
  8. Bouhon EJR, Keith DJ, Parkin IAP, Sharpe AG, Lydiate D (1996) Alignment of conserved C genomes of Brassica oleracea and B. napus. Theor Appl Genet 93: 833–839CrossRefGoogle Scholar
  9. Bowers JE, Chapman BA, Rong JK, Paterson AH (2003) Unraveling angiosperm chromosome evolution by analysis of duplication/diploidization events. Nature 422: 433–438PubMedCrossRefGoogle Scholar
  10. Brunel D, Froger N, Pelletier G (1999) Development of amplified consensus genetic markers (ACGM) in Brassica napus from Arabidopsis thaliana sequences of known biological function. Genome 42: 387–402PubMedGoogle Scholar
  11. Butruille DV, Guries RP, Osborn TC (1999) Linkage analysis of molecular markers and quantitative trait loci in populations of inbred backcross lines of Brassica napus L. Genetics 153:949– 964Google Scholar
  12. Cai WW, Reneker J, Chow CW, Vaishnav M, Bradley A (1998) An anchored framework BAC map of mouse chromosome 11 assembled using multiplex oligonucleotide hybridization. Genomics 54: 387–397PubMedCrossRefGoogle Scholar
  13. Chalhoub B, Budin K, Aubourg S, Lecharny A, Belcram H (2002) Development of a BAC library and comparative genomic approaches for high throughput physical and genetic mapping of oilseed rape. 13th Crucifer Genetics Workshop, University of California, DavisGoogle Scholar
  14. Cheung WY, Champagne G, Hubert N, Landry BS (1997a) Comparison of the genetic maps of Brassica napus and Brassica oleracea. Theor Appl Genet 94: 569–582CrossRefGoogle Scholar
  15. Cheung WY, Friesen L, Rakow GFW, Seguin-Swartz G, Landry BS (1997b) A RFLP-based linkage map of mustard [Brassica juncea (L) Czern and Coss]. Theor Appl Genet 94:569– 582Google Scholar
  16. Cloutier S, Cappadocia M, Landry BS (1995) Study of microspore culture responsiveness (Bras-sica napus L.) by comparative mapping of an F2 population and to microspore derived populations. Theor Appl Genet 91: 841–847CrossRefGoogle Scholar
  17. Foisset N, Delourme R, Barret P, Hubert N, Landry BS, Renard M (1996) Molecular mapping analysis of Brassica napus using isozyme, RAPD and RFLP markers on double haploid progeny. Theor Appl Genet 93: 1017–1025Google Scholar
  18. Fourmann M, Froger N, Pelletier G, Brunel D (1998) Amplified consensus gene markers (ACGM): tools designing for a genetic maps of known function genes. Plant Animal Genome VI, San Diego, CA, abstract no P215, p 132Google Scholar
  19. Fourmann M, Charlot F, Froger N, Delourme R, Brunel D (2001) Expression, mapping, and genetic variability of Brassica napus disease resistance gene analogues. Genome 44: 1083–1099PubMedGoogle Scholar
  20. Fukui K (2003) Brassica and legume chromosomes. In: Nagata T, Tabata S (eds) Biotechnology in agricultural and forestry, vol. 52. Brassicas and legumes. Springer, Berlin Heidelberg New York, pp 114–141Google Scholar
  21. Giovannelli JL, Farnham MW, Wang M, Strand AE (2002) Development of sequence characterized amplified region markers linked to downy mildew resistance in broccoli. J Am Soc Hortic Sci 127: 597–602Google Scholar
  22. Hall EA, Fiebig A, Preuss D (2002) Beyond the Arabidopsis genome: opportunities for comparative genomics. Plant Physiol 129: 1439–1447PubMedCrossRefGoogle Scholar
  23. Hasterok R, Jenkins G, Langdon T, Jones RN, Maluszynska J (2001) Ribosomal DNA is an effective marker of Brassica chromosomes. Theor Appl Genet 103: 486–490CrossRefGoogle Scholar
  24. Heneen WK, Brismar K (2001) Maternal and embryonal control of seed colour by different Bras-sica alboglabra chromosomes. Plant Breed 120: 325–329CrossRefGoogle Scholar
  25. Heneen WK, Jorgensen RB (2001) Cytology, RAPD, and seed colour of progeny plants from Bras-sica rapa-alboglabra aneuploids and development of monosomic addition lines. Genome 44: 1007–1021PubMedGoogle Scholar
  26. Hoenecke M, Chyi YS (1991) Comparison of Brassica napus and B. rapa genomes based on restriction fragment length polymorphism mapping. In: Rapeseed in a changing world. Proceedings of the 8th International Rapeseed Congress 4. Saskatoon, Saskatchewan, Canada,pp 1102–1107Google Scholar
  27. Howell EC, Barker GC, Jones GH, Kearsey MJ, King GJ, Kop EP, Ryder CD, Teakle GR, Vicente JG, Armstrong SJ (2002) Integration of the cytogenetic and genetic linkage maps of Brassica oleracea. Genetics 161: 1225–1234PubMedGoogle Scholar
  28. Jackson S, Cheng Z, Wang ML, Goodman H, Jiang JM (2000) Comparative fluorescence in situ hybridization mapping of a 431 kb Arabidopsis thaliana bacterial artificial chromosome contig reveals the role of chromosomal duplications in the expansion of the Brassica rapa genome. Genetics 156: 833–838PubMedGoogle Scholar
  29. Kaneko Y, Yano H, Bang SW, Matsuzawa Y (2001) Production and characterization of Raphanus sativus-Brassica rapa monosomic chromosome addition lines. Plant Breed 120: 163–168CrossRefGoogle Scholar
  30. Kaneko Y, Nagasawa N, Bang SW, Matsuzawa Y (2002) Homoeological relationships between the f chromosome of Brassica rapa and the e chromosome of Brassica oleracea. Plant Breed 121: 171–173CrossRefGoogle Scholar
  31. Kianian SF, Quiros CF (1992) Generation of a Brassica oleracea composite RFLP map: linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84: 544–554CrossRefGoogle Scholar
  32. King G, Bancroft I, Barker G, Beckett P, Beyton J, van den Boogaart T, Clissold L (2002) Physical mapping of the Brassica A and C genomes. 13th Crucifer Genetics Workshop, University of California, DavisGoogle Scholar
  33. Kole C, Williams PH, Rimmer SR, Osborn TC (2002) Linkage mapping of genes controlling resistance to white rust (Albugo candida) in Brassica rapa (syn. campestris) and comparative mapping to Brassica napus and Arabidopsis thaliana. Genome 45: 22–27PubMedCrossRefGoogle Scholar
  34. Kowalski SP, Lan TH, Feldmann KA, Paterson AH (1994) Comparative mapping of Arabidopsis thaliana and Brassica oleracea chromosomes reveals islands of conserved organization. Genetics 138: 1–12Google Scholar
  35. Lakshmikumaran M, Das S, Srivastava PS (2003) Application of molecular markers in Brassica coenospecies: comparative mapping and tagging. In: Nagata T, Tabata S (eds) Biotechnology in agricultural and forestry, vol 52. Brassicas and legumes. Springer, Berlin Heidelberg New York, pp 37–68Google Scholar
  36. Lan TH, Paterson AH (2000) Comparative mapping of quantitative trait loci sculpting the curd of Brassica oleracea. Genetics 155: 1927–1954PubMedGoogle Scholar
  37. Lan TH, Paterson AH (2001) Comparative mapping of QTLs determining the plant size of Bras-sica oleracea. Theor Appl Genet 103: 383–397CrossRefGoogle Scholar
  38. Lan TH, Del Monte TA, Reischmann KP, Hyman J, Kowalski SP (2000) An EST-enriched compar- ative map of Brassica oleracea and Arabidopsis thaliana. Genome Res 10: 776–788PubMedCrossRefGoogle Scholar
  39. Leroy XJ, Leon K, Branchard M (2000) Characterisation of Brassica oleracea L. by microsatellite primers. Plant Syst Evol 225: 235–240CrossRefGoogle Scholar
  40. Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103: 455–461CrossRefGoogle Scholar
  41. Li GY, Riaz A, Goyal S, Abel S, Quiros CF (2001) Inheritance of three major genes involved in the synthesis of aliphatic glucosinolates in Brassica oleracea. J Am Soc Hortic Sci 126:427– 431Google Scholar
  42. Li GY, Gao M, Yang B, Quiros CF (2003) Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor Appl Genet 107: 168–180PubMedCrossRefGoogle Scholar
  43. Lim YP, Kim JH, Bang JW, Nam HG, Cho KW, Jang CS (1998) Genetic mapping of Chinese cabbage (Brassica rapa L. var. pekinensis). Plant Animal Genome VI, Abstract no P221, p 133Google Scholar
  44. Lim YP, Kim JH, Bang JW, Hur YK, Choi KS, Kim HK, Lee HS, Kim HI (2002) Brassica rapa genome research programs in Korea.13th Crucifer Genetics Workshop, University of California, DavisGoogle Scholar
  45. Liu R, Qian W, Meng J (2002) Association of RFLP markers and biomass heterosis in trigenomic hybrids of oilseed rape (Brassica napus x B. campestris). Theor Appl Genet 105: 1050–1057PubMedCrossRefGoogle Scholar
  46. Lombard V, Delourme R (2001) A consensus linkage map for rapeseed (Brassica napus L.): construction and integration of three individual maps from DH populations. Theor Appl Genet 103: 491–507CrossRefGoogle Scholar
  47. Lowe A, Jones AE, Raybould AF, Trick M, Moule C, Edwards K (2002) Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle. Mol Ecol Notes 2: 7–11CrossRefGoogle Scholar
  48. Lukens L, Zou F, Lydiate D, Parkin I, Osborn T (2003) Comparison of a Brassica oleracea genetic map with the genome of Arabidopsis thaliana. Genetics 164: 359–372PubMedGoogle Scholar
  49. Lydiate D, Sharpe A, Lagercrantz U, Parkin I (1993). Mapping the Brassica genome. Outlook Agric 2: 85–92Google Scholar
  50. McGrath JM, Jansco MM, Pichersky E (1993) Duplicate sequences with similarity to expressed genes in the genome of Arabidopsis thaliana. Theor Appl Genet 86: 880–888CrossRefGoogle Scholar
  51. O’Neill C, Bancroft I (2000) Comparative physical mapping of the genome of Brassica oleracea var. alboglabra that are homologous to sequenced regions of chromosome 4 and 5 of Arabidopsis thaliana. Plant J 23: 233–243PubMedCrossRefGoogle Scholar
  52. Osborn TC, Lukens L (2003) The molecular genetic basis of flowering time variation in Brassica species. In: Nagata T, Tabata S (eds) Biotechnology in agricultural and forestry, vol 52. Brassicas and legumes. Springer, Berlin Heidelberg New York, pp 69–86Google Scholar
  53. Parkin IAP, Sharpe AG, Keith DJ, Lydiate DJ (1995) Identification of the A and C genomes of amphidiploid Brassica napus (oilseed rape). Genome 38: 1122–1131PubMedCrossRefGoogle Scholar
  54. Paterson AH, Bowers JE, Burow MD, Draye X, Elsik CG, Jiang C, Katsar C, Lan T, Lin Y (2000) Comparative genomics of plant chromosomes. Plant Cell 12: 1523–1539PubMedGoogle Scholar
  55. Paterson A, Quiros CF, Lala H, Bowers J, Lee S, Bethel C (2002) Hybridization-based physical mapping of the Brassica genome.13th Crucifer Genetics Workshop, University of California, DavisGoogle Scholar
  56. Plieske J, Struss D (2001) Microsatellite markers for genome analysis in Brassica. I. Development in Brassica napus and abundance in Brassicaceae species. Theor Appl Genet 102: 689–694CrossRefGoogle Scholar
  57. Quiros CF (2001) DNA-based marker Brassica maps. In: Phillips RL, Vasil IK (eds) Advances in cellular and molecular biology of plants, vol I. DNA based markers in plants. Kluwer, Dordrecht, pp 201–238Google Scholar
  58. Quiros CF, Grellet F, Sadowski J, Suzuki T, Wrobelwski T (2001) Arabidopsis and Brassica comparative genomics: sequence, structure and gene content in the ABI1-Rps2-Ck1 chromosomal segment and related regions. Genetics 157: 1321–1330Google Scholar
  59. Ramsay LD, Jennings DE, Bohuon EJR, Arthur AE, Lydiate DJ, Kearsey MJ, Marshall DF (1996) The construction of a substitution library of recombinant backcross lines in Brassica oleracea for the precision mapping of quantitative loci. Genome 39: 558–567PubMedCrossRefGoogle Scholar
  60. Schmidt R, Acarkan A, Boivin K, Clarenz O, Rossberg M (2003) The sequence of the Arabidopsis genome as a tool for comparative structural. genomics in Brassicaceae. In: Nagata T, Tabata S (eds) Biotechnology in agricultural and forestry, vol 52. Brassicas and legumes. Springer, Berlin Heidelberg New York, pp 19–38Google Scholar
  61. Schrader O, Budahn H, Ahne R (2000) Detection of 5S and 25S rRNA genes in Sinapis alba, Raphanus sativus and Brassica napus by double fluorescence in situ hybridization. Theor Appl Genet 100: 665–669CrossRefGoogle Scholar
  62. Sebastian RL, Howell EC, King GJ, Marshall DF, Kearsey MJ (2000) An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations. Theor Appl Genet 100: 75–81CrossRefGoogle Scholar
  63. Sebastian RL, Kearsey MJ, King GJ (2002) Identification of quantitative trait loci controlling developmental characteristics of Brassica oleracea L. Theor Appl Genet 104: 601–609PubMedCrossRefGoogle Scholar
  64. Sharma A, Mohopatra T, Sharma RP (1994) Molecular mapping and character tagging in Bras-sica juncea. 1. Degree, nature and linkage relationship of RFLPs and their association with quantitative traits. J Plant Biochem Biotechnol 3: 85–89Google Scholar
  65. Sharma R, Aggarwal RAK, Kumar R, Mohapatra T, Sharma RP (2002) Construction of an RAPD linkage map and localization of QTLs for oleic acid level using recombinant inbreds in mustard (Brassica juncea). Genome 45: 467–472PubMedCrossRefGoogle Scholar
  66. Sharpe AG, Parkin IAP, Keith DJ, Lydiate DJ (1995) Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome 38: 1112–1121PubMedCrossRefGoogle Scholar
  67. Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80: 57–64CrossRefGoogle Scholar
  68. Snowdon RJ, Friedrich T, Friedt W, Kohler W (2002) Identifying the chromosomes of the A- and C-genome diploid Brassica species B. rapa (syn. campestris) and B. oleracea in their amphidiploid B. napus. Theor Appl Genet 104: 533–538PubMedCrossRefGoogle Scholar
  69. Sukabe K, Iketani H, Nunome T, Kage T, Hirai M (2002). Isolation and characterization of microsatellites in Brassica rapa L. Theor Appl Genet 104: 1092–1098CrossRefGoogle Scholar
  70. Uzunova M, Ecke W, Weissleder K, Robbelen G (1995) Mapping the genome of rapeseed (Bras-sica napus L). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor Appl Genet 90: 194–204Google Scholar
  71. Vicente JG, King GJ (2001) Characterisation of disease resistance gene-like sequences in Brassica oleracea L. Theor Appl Genet 102: 555–563CrossRefGoogle Scholar
  72. Vision TJ, Brown DG, Tanksley SD (2000) The origins of genomic duplications in Arabidopsis. Science 290: 2114–2117PubMedCrossRefGoogle Scholar
  73. Voorips RE, Jongerius MC, Kanne HJ (2003) Quantitative trait loci for clubroot resistance in Bras-sica oleracea. In: Nagata T, Tabata S (eds) Biotechnology in agricultural and forestry, vol 52. Brassicas and legumes, Springer, Berlin Heidelberg New York, pp 87–104Google Scholar
  74. Wroblewski T, Coulibaly S, Sadowski J, Quiros CF (2000) Variation and phylogenetic utility of the Arabidopsis thaliana Rps2 homolog in various species of the tribe Brassicaceae. Mol Phylogenet Evol 16: 440–448PubMedCrossRefGoogle Scholar
  75. Xu FS, Wang YH, Meng J (2001) Mapping boron efficiency gene(s) in Brassica napus using RFLP and AFLP markers. Plant Breed 120: 319–324CrossRefGoogle Scholar
  76. Zhang HB (1998) The Texas A M BAC Center: Ten new libraries added in 1997. Plant Animal Genome VI, San Diego, CA, Abstract no P15, p 80Google Scholar
  77. Ziolkowski PA, Sadowski J (2002) FISH-mapping of rDNAs and Arabidopsis BACs on pachytene complements of selected Brassicas. Genome 45: 189–197PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • C. F. Quiros
    • 1
  • A. H. Paterson
    • 2
  1. 1.Department of Vegetable CropsUniversity of CaliforniaDavisUSA
  2. 2.Plant Genome Mapping LaboratoryUniversity of GeorgiaAthensUSA

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