Application of Protein Electrophoresis Techniques

  • Alan H. Goldstein
Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)


As stated in the editor’s introduction, the purpose of this book is to emphasize recent developments in biotechnology and biomaterials, including fundamental concepts and novel experimental techniques. However, it is often the case that, as an area of knowledge evolves, concepts once considered esoteric may become fundamental. Likewise, experimental techniques that are routine in one area of science may be quite novel when applied to another. Such is the case with protein electrophoresis, a routine technique in molecular cell biology based on fundamental concepts in protein biochemistry. Biomaterials scientists have always recognized that protein-mediated phenomena were of great importance to their field (Ratner et al., 1996). However, as the title of this volume emphasizes, given the evolution of the field toward biotechnology-based biomaterials applications, one could argue that the role of proteins will become preeminent. Within this context, the electrophoretic characterization of protein interactions with nonviable biomaterials, either the result of unavoidable adsorption or through biorational design, will become a vital tool in the arsenal of many biomaterials scientists.


Adsorbed Protein Biomaterial Scientist Electrophoretic Separation Apparent Molecular Mass Protein Electrophoresis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Balsubramanian, V., N.K. Grusin, R.W. Bucher, V.T. Turitto, S.M. Slack. J. Biomed. Mater. Sci. 44: 233 (1999)Google Scholar
  2. Becton Dickinson Product Specification Sheet. 40234 — 40319 (1997)Google Scholar
  3. Branden, C., J. Tooze. Introduction to Protein Structure 2nd ed.. Garland, New York, pp. 1–33 (1999)Google Scholar
  4. Bockris, J. O’m., A. K. Reddy. Modern Electrochemistry. Plenum Press, New York (2000)Google Scholar
  5. Chevally, B., N.Abdul-Malak, D. Herbage. J. Biomed. Mater. Sci., 49: 448 (2000)Google Scholar
  6. Faucheux, N., B. Haye, M.D. Nagel. Biomaterials, 21: 10 (2000)CrossRefGoogle Scholar
  7. Giraud-Guille, M.-M., L. Besseau, C. Chopin, P. Durand, D. Herbage. Biomaterials 21: 899 (2000)CrossRefGoogle Scholar
  8. Goldstein, A.H.. Theor. Appl. Genet. 82 (2): 191 (1991)Google Scholar
  9. Goldstein, A. H., J. S. Salesky. Proc. 102nd Annual meeting America Ceramic Society, ACevS Press, Ohio (1999)Google Scholar
  10. Goldstein, A.H.. J.S. Salesky, in Surface-Active Processes In Materials: The Larry Hench Symposium. eds. D.E. Clark, et al.. Ceramic Transactions 101(The American Ceramic Society, Westerville (2000)Google Scholar
  11. Hames, B.D.. Gel Electrophoresis of Proteins. In B.D. Hames, D. Rickwood. IRL Press, Washington DC, 1–86 (1986)Google Scholar
  12. Hames, B.D., D. Rickwood. Gel Electrophoresis of Proteins. IRL Press, Washington DC (1986)Google Scholar
  13. Horbett, T.A.. An introduction to materials in medicine In: B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons, eds., Biomaterials Science. Academic Press, New York, 136 (1996)Google Scholar
  14. Jenny, C.R., J.M. Anderson. J. Biomed. Mater. Sci. 50: 3281 (2000)Google Scholar
  15. Laemmli, U.K. Nature 227: 680 (1970)ADSCrossRefGoogle Scholar
  16. Lodish, H, D. Baltimore, A. Berk, S.L. Zipursky, P. Matsudaira, J. Darnell. Molecular Cell Biology, 3rd ed. W.H. Freeman, New York, pp.1123 (1995)Google Scholar
  17. McClung, W.G., D.L. Clapper, S.-P. Hu, J.L. Brash. J. Biomed. Mater. Sci. 49: 409 (2000)Google Scholar
  18. Neubauer, G., M. Mann. Anal. Chem. 71: 235 (1999)CrossRefGoogle Scholar
  19. Norde, W., C.E. Giacomelli. J. Biotechnol. 79: 259 (2000)CrossRefGoogle Scholar
  20. O’Farrel, P.H. J. Biol. Chem. 254: 4007 (1975)Google Scholar
  21. Rappsilber, J., S. Siniossoglou, E. C. Hurt, M. Mann. Anal. Chem. 72: 267 (2000)CrossRefGoogle Scholar
  22. Ratner, B. D., A.S. Hoffman, F.J. Schoen, J.E. Lemons, eds., Biomaterials Science: An Introduction to Materials in Medicine. Academic Press, New York (1996)Google Scholar
  23. Salesky, J. S., A.H. Goldstein. Proc. Xth Int. Symp. on Non-Oxide Glasses. America Ceramic Society, ACevS Press, Westerville Ohio (2000)Google Scholar
  24. Sambrook, J., E.F. Fritsch, T. Maniatis. Molecular Cloning, 2nd ed. Cold Spring Harbor (1989)Google Scholar
  25. Sammons, D.W., L.D. Adams, T.J. Vidmar, C.A. Hatfield, D.H. Jones, P.J. Chuba, S.W. Crooks. In: Two-Dimensional Gel Electrophoresis of Proteins. J.E. Celos and R. Bravo, eds. Academic Press, New York, 112 (1984)Google Scholar
  26. Satulovsky, J., M.A. Carignano, I. Szleifer. Proc. Nat. Acad. Sci. USA 97: 9037 (2000)ADSCrossRefGoogle Scholar
  27. Sun, X., H. Sheardown, P.Tengvall, J. Brash. J. Biomed. Mater. Sci. 49: 66 (2000)CrossRefGoogle Scholar
  28. Switzer, R. C., C.R. Merril, S. Shifrin. Anal. Biochem. 98: 231 (1979)CrossRefGoogle Scholar
  29. Tanner J., K. Pekka, P. Vallittu, E. Soderling. J. Biomed. Mater. Sci. 49: 250 (2000)Google Scholar
  30. Trans. of the Sixth World Biomater. Congr., Society for Biomaterials, Minneapolis, MNGoogle Scholar
  31. Voet, D., J. G. Voet. Biochemistry, 2nd ed. John Wiley & Sons, New York (1995)Google Scholar
  32. Zhdanov, V. P., B. Kasemo. Proteins 40: 339 (2000)CrossRefGoogle Scholar

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  • Alan H. Goldstein

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