Abstract
The amdS gene of Aspergillus nidulans encodes an acetamidase enzyme that hydrolyses acetamide and a limited range of other amides. This allows acetamide to be used as a sole source of nitrogen or as a source of carbon. As far as we know, this gene plays no role with respect to the physiology or development of A. nidulans since deletion mutants of amdS have no detectable phenotype other than an inability to catabolize acetamide (Hynes 1979). It should also be noted that acetamide is a poor sole source of carbon or nitrogen for A. nidulans and it is unclear whether acetamide would be a significant source of metabolites in the natural environment. However, genes with significant similarity to amdS have been found in other Aspergillus spp. (J.A. Sharp, unpubl. data) and in other organisms in the course of recent genome sequencing projects.
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References
Andrianopoulos A, Hynes MJ (1988) Cloning and analysis of the positively acting regulatory gene amdR from Aspergillus nidulans. Mol Cell Biol 8:3532–3541
Andrianopoulos A, Hynes MJ (1990) Sequence and functional analysis of the positively acting regulatory gene amdR from Aspergillus nidulans. Mol Cell Biol 10: 3194–3203
Andrianopoulos A, Brons J, Davis MA, Hynes MJ (1997) The amdA regulatory gene of Aspergillus nidulans: characterization of gain-of-function mutations and identification of binding sites for the gene product. Fungal Genet Biol 21:50–63
Apirion D (1965) The two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in Aspergillus nidulans. Genet Res (Cambr) 6:317–329
Armitt S, McCullough W, Roberts CF (1976) Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans. J Gen Microbiol 92:263–282
Arst HN (1976) Integrator gene in Aspergillus nidulans. Nature 262:231–234
Arst HN, Cove DJ (1973) Nitrogen metabolite repression in Aspergillus nidulans. Mol Gen Genet 126:111–141
Arst HN, Scazzocchio C (1985) Formal genetics and molecular biology of the control of gene expression in Aspergillus nidulans. In: Bennett JW, Lasure LL (eds) Gene manipulations in fungi. Academic Press, New York, pp 309–343
Arst HN, Penfold HA, Bailey CR (1979) Lactam utilization in Aspergillus nidulans: evidence for a fourth gene under the control of the integrator gene intA. Mol Gen Genet 166:312–327
Atkinson PW, King JA, Hynes MJ (1985) Identification of the aciA gene controlled by the amdA regulatory gene in Aspergillus nidulans. Curr Genet 10:133–138
Ballance DJ, Turner G (1986) Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD). Mol Gen Genet 202:271–275
Brakhage AA, Andrianopoulos A, Kato M, Steidl S, Davis MA, Tsukagoshi N, Hynes MJ (1999) HAP-like CCAAT-binding complexes in filamentous fungi: implications for biotechnology. Fungal Genet Biol 27:243–252
Brammar WJ, Clarke PJ (1964) Induction and repression of Pseudomonas aeruginosa amidase. J Gen Microbiol 37:307–319
Caddick MX, Arst HN, Taylor LH, Johnson RI, Brownlee AG (1986) Cloning of the regulatory gene are A mediating nitrogen metabolite repression in Aspergillus nidulans. EMBO J 5:1087–1090
Corrick CM, Twomey AP, Hynes MJ (1987) The nucleotide sequence of the amdS gene of Aspergillus nidulans and the molecular characterization of 5’ mutations. Gene 53:67–71
Cove DJ (1979) Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev 54:291–327
Cubero B, Scazzocchio C (1994) Two different, adjacent and divergent zinc finger binding sites are necessary for CreA mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J 13:407–415
Davis MA, Cobbett CS, Hynes MJ (1988) An amdS-lacZ fusion for studying gene regulation in Aspergillus. Gene 63:199–212
Dowzer CEA, Kelly JM (1989) Cloning of the creA gene from Aspergillus nidulans: a gene involved in carbon catabolite repression. Curr Genet 15:457–459
Dowzer CEA, Kelly MJ (1991) Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Mol Cell Biol 11:5701–5709
Espeso EA, Arst HN Jr (2000) On the mechanism by which alkaline pH prevents expression of an acid-expressed gene. Mol Cell Biol 20:3355–3363
Fincham JRS (1989) Transformation in fungi. Microbiol Rev 53:148–170
Fraser J, Davis MA, Hynes MJ (2001) The formamidase gene of Aspergillus nidulans: regulation by nitrogen metabolite repression and transcriptional interference by an overlapping upstream gene. Genetics 157: 119–131
Fraser J, Davis MA, Hynes MJ (2002) The genes gmdA, encoding an amidase, and bzuA, encoding a cytochrome P450, are required for benzamide utilisation in Aspergillus nidulans. Fungal Genet Biol 35: 135–146
Gems D, Johnstone IL, Clutterbuck AJ (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98:61–67
Gomi K, Kitamoto K, Kumagi C (1991) Cloning and molecular characterisation of the acetamidase-encoding gene (amdS) from Aspergillus oryzae. Gene 108:183–208
Hamer JE, Timberlake WE (1987) Functional organization of the Aspergillus nidulans trpC promoter. Mol Cell Biol 7:2352–2359
Hutchings H, Stahmann K-P, Roels S, Espeso EA, Timber-lake WE, Arst HN Jr, Tilburn J (1999) The multiply-regulated gabA gene encoding the GABA permease of Aspergillus nidulans: a score of exons. Mol Microbiol 32(3):557–568
Hynes MJ (1970) Induction and repression of amidase enzymes in Aspergillus nidulans. J Bacteriol 103:482–487
Hynes MJ (1975a) Amide utilization in Aspergillus nidulans: evidence for a third amidase enzyme. J Gen Microbiol 91:99–109
Hynes MJ (1975b) Studies on the role of the are A gene in the regulation of nitrogen catabolism in Aspergillus nidulans. Aust J Biol Sci 28:301–313
Hynes MJ (1975c) A ds-dominant regulatory mutation affecting enzyme induction in the eukaryote Aspergillus nidulans. Nature 253:210–212
Hynes MJ (1977) Induction of the acetamidase of Aspergillus nidulans by acetate metabolism. J Bacteriol 131:770–775
Hynes MJ (1978a) An “up-promoter” mutation affecting the acetamidase of Aspergillus nidulans. Mol Gen Genet 166:31–36
Hynes MJ (1978b) Multiple independent control mechanisms affecting the acetamidase of Aspergillus nidulans. Mol Gen Genet 161:59–65
Hynes MJ (1979) Fine structure mapping of the acetamidase structural gene and its controlling region in Aspergillus nidulans. Genetics 91:381–392
Hynes MJ (1980) A mutation adjacent to gene amdS, defining the site of action of positive control gene amdR in Aspergillus nidulans. J Bacteriol 142:400–406
Hynes MJ (1982) A ds-dominant mutation in Aspergillus nidulans affecting the expression of the amdS gene in the presence of mutations in the unlinked gene, amdA. Genetics 102:139–147
Hynes MJ, Kelly JM (1977) Pleiotropic mutants of Aspergillus nidulans altered in carbon metabolism. Mol Gen Genet 150:193–204
Hynes MJ, Pateman JA (1970a) The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. I. Mutants able to utilize acrylamide. Mol Gen Genet 108:97–106
Hynes MJ, Pateman JA (1970b) The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. II. Mutants resistant to fluoroacetamide. Mol Gen Genet 108:107–116
Hynes MJ, Corrick CM, King JA (1983) Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Mol Cell Biol 3:1430–1439
Hynes MJ, Corrick CM, Kelly JM, Littlejohn TG (1988) Identification of the sites of action for regulatory genes controlling the amdS gene of Aspergillus nidulans. Mol Cell Biol 8:2589–2596
Katz ME, Hynes MJ (1989a) Isolation and analysis of the acetate regulatory gene, facB, from Aspergillus nidulans. Mol Cell Biol 9:5696–5701
Katz ME, Hynes MJ (1989b) Characterization of the amd.R-controlled lamA and lamB genes of Aspergillus nidulans. Genetics 122:331–339
Katz ME, Saleeba JA, Sapats SI, Hynes MJ (1990) A mutation affecting amdS expression in Aspergillus nidulans contains a triplication of a ds-acting regulatory sequence. Mol Gen Genet 220:373–376
Kelly JM, Hynes MJ (1977) Increased and decreased sensitivity to carbon catabolite repression of enzymes of acetate metabolism in mutants of Aspergillus nidulans. Mol Gen Genet 156:87–92
Kelly JM, Hynes MJ (1985) Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans. EMBO J 4:475–479
Kelly JM, Hynes MJ (1987) Multiple copies of the amdS gene of Aspergillus nidulans cause titration of trans-acting regulatory proteins. Curr Genet 11:21 –31
Lints R, Davis MA, Hynes MJ (1995) The positively acting amdA gene of Aspergillus nidulans encodes a protein with two C2H2 zinc-finger motifs. Mol Microbiol 15: 965–975
Littlejohn TG, Hynes MJ (1992) Analysis of the site of action of the amdR product for regulation of the amdS gene of Aspergillus nidulans. Mol Gen Genet 235:81–88
Lockington RA, Kelly JM (2002) The WD40-repeat protein CreC interacts with and stabilizes the deubiquitinat-ing enzyme CreB in vivo in Aspergillus nidulans. Mol Microbiol 43(5):1173–1182
Mamorstein R, Carey M, Ptashne M, Harrison SC (1992) DNA recognition by GAL4: structure of a protein-DNA complex. Nature 356:408–414
Maquat LE, Carmichael GG (2001) Quality control of mRNA function. Cell 104:173–176
Marzluf GA (1997) Genetic regulation of nitrogen metabolism in the fungi. Microbiol Mol Biol Rev 61:17–32
Murphy RL, Andrianopoulos A, Davis MA, Hynes MJ (1997) Identification of amdX, a new C2H2 zinc finger gene involved in the regulation of the amdS gene of Aspergillus nidulans. Mol Microbiol 23:591–602
Narendja FM, Davis MA, Hynes MJ (1999) AnCF, the CCAAT binding complex of Aspergillus nidulans is essential for the formation of a DNase I hypersensitive site in the 5′ region of the amdS gene. Mol Cell Biol 19:6523–6531
Osherov N, May GS (2000) Conidial germination in Aspergillus nidulans requires RAS signalling and protein synthesis. Genetics 155:647–656
Papagiannopoulos P, Andrianopoulos A, Sharp JA, Davis MA, Hynes MJ (1996) Deletion of the hapC gene of Aspergillus nidulans, a homologue of the yeast HAP3 gene, results in the loss of CCAAT binding to the amdS and gat A promoters. Mol Gen Genet 251:412–421
Parsons LM, Davis MA, Hynes MJ (1992) Identification of functional regions of the positively acting regulatory gene amdR from Aspergillus nidulans. Mol Microbiol 6:2999–3007
Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton AWJ (1953) The genetics of Aspergillus nidulans. Adv Genet 5:141–238
Punt PJ, Dingemanse MA, Kuyvenhoven A, Soede RDM, Pouwels PH, van den Hondel CAMJJ (1990) Functional elements in the promoter region of the Aspergillus nidulans gpdA gene, encoding glyceraldehyde-3-phosphate dehydrogenase. Gene 93:101–109
Ravagnani A, Gorfinkiel L, Langdon T, Diallinas G, Adjaji E, Demais S, Gorton D, Arst HN Jr, Scazzocchio C (1997) Subtle hydrophobic interactions between the seventh residue of the zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA. EMBO J 16:3974–3986
Richardson IB, Hurley SK, Hynes MJ (1989) Cloning and molecular characterization of the amdR controlled gatA gene of Aspergillus nidulans. Mol Gen Genet 217:118–125
Richardson IB, Katz ME, Hynes MJ (1992) Molecular characterization of the lam locus and sequences involved in regulation by the AmdR protein of Aspergillus nidulans. Mol Cell Biol 12:337–346
Saleeba JA, Cobbett CS, Hynes MJ (1992) Characterization of the amdA-regulated aciA gene of Aspergillus nidulans. Mol Gen Genet 235:349–358
Sandeman RA, Hynes MJ (1989) Isolation of the facA (acetyl-Co enzyme A synthetase) and acuE (malate synthase) genes of Aspergillus nidulans. Mol Gen Genet 218:87–92
Scazzocchio C (2000) The fungal GATA factors. Curr Opin Micro 3:126–131
Shroff RA, O’Connor SM, Hynes MJ, Lockington RA, Kelly JM (1997) Null alleles of ere A, the regulator of carbon catabolite repression in Aspergillus nidulans. Fungal Genet Biol 22:28–38
Small AJ, Hynes MJ, Davis MA (1999) The TamA protein fused to a DNA binding domain can recruit AreA, the major nitrogen regulatory protein, to activate gene expression in Aspergillus nidulans. Genetics 153:95–105
Steidl S, Papagiannopoulos P, Litzka O, Andrianopoulos A, Davis MA, Brakhage AA, Hynes MJ (1999) AnCF, the CCAAT binding complex of Aspergillus nidulans, contains products of the hapB, hapC, and hapE genes and is required for activation by the pathway-specific regulatory gene amdR. Mol Cell Biol 19:99–106
Stemple CJ, Davis MA, Hynes MJ (1998) The facC gene of Aspergillus nidulans encodes an acetate-inducible carnitine acetyltransferase. J Bacteriol 180:6242–6251
Tilburn J, Scazzocchio C, Taylor GG, Upshall A, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Transformation by integration in Aspergillus nidulans. Gene 26:205–221
Todd RB, Andrianopoulos A (1997) Evolution of a fungal regulatory gene family: the Zn(II)2Cys6 binuclear cluster DNA binding motif. Fungal Genet Biol 21:388–405
Todd R, Murphy RL, Martin HM, Sharp JA, Davis MA, Katz ME, Hynes MJ (1997) The acetate regulatory genefacB of Aspergillus nidulans encodes a Zn(II)2Cys6 transcriptional activator. Mol Gen Genet 254:495–504
Todd RB, Andrianopoulos A, Davis MA, Hynes MJ (1998) FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences. EMBO J 17:2042–2054
Van Heeswijck R, Hynes MJ (1991) The amdR product and a CCAAT-binding factor bind to adjacent, possibly overlapping DNA sequences in the promoter region of the Aspergillus nidulans amdS gene. Nucleic Acids Res 19:2655–2660
Wilson RA, Arst HN Jr (1998) Mutational analysis of AreA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the “streetwise” GATA family of transcription factors. Microbiol Mol Biol Rev 62:586–596
Yelton MM, Timberlake WE, Van den Hondel CAMJJ (1985) A cosmid for selecting genes by complementation in Aspergillus nidulans: selection of the developmentally regulated yA locus. Proc Natl Acad Sci USA 82:834–838
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Hynes, M.J., Davis, M.A. (2004). Regulation of the amdS Gene in Aspergillus nidulans . In: Brambl, R., Marzluf, G.A. (eds) Biochemistry and Molecular Biology. The Mycota, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06064-3_21
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DOI: https://doi.org/10.1007/978-3-662-06064-3_21
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