Advertisement

Effects of High-pressure Gas on the Growth Thermograms of Yeast

  • T. Arao
  • Y. Muramoto
  • Y. Suzuki
  • K. Tamura
Conference paper

Abstract

Using a microcalorimeter the heat evolved during incubation of yeast cultures at 30oC was detected in the form of growth thermogram (metabolic heat — incubation time curve). Correlation of the heat evolution curves with the number of cells and the turbidity of the culture was found to be very good. In this study, the effects of high-pressure gas in open system on the growth curve of yeast were investigated and the inhibition action of the gases (nitrogen, air, nitrous oxide and argon) on the growth was quantitatively assayed using Biothermo Analyzer. The growth thermograms were used to estimate microbial activity of yeast under compressed gases and to determine minimum inhibitory pressure (MIP) that is regarded as an index of toxic potency of dissolved gases. It was found that the increase of pressure induced clear inhibitory action on yeast cells, especially for air. The order of the action was as follows: air > N2O > Ar > N2.

Keywords

Nitrous Oxide Logarithmic Phase Yeast Culture Growth Rate Constant Heat Output 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    K. Takahashi, Thermochimica Acta, 163 (1990) 71.CrossRefGoogle Scholar
  2. [2]
    O. A. Antoce, V. Antoce, K. Takahashi and F. Yoshizako, Biosci. Biotech. Biochem. 61 (1997) 664.CrossRefGoogle Scholar
  3. [3]
    K. Tamura, Y. Kamiki and M. Miyashita, “Advances in High Pressure Bioscience and Biotechnology”, ed. H. Ludwig, Springer (1999) p. 47.CrossRefGoogle Scholar
  4. [4]
    T. Arao and K. Tamura, Prog. Aneth. Mechanism, 6 (2000) 496.Google Scholar
  5. [5]
    H. Iwahashi, Nippon nogeikagaku kaishi, 74 (2000) 609.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • T. Arao
    • 1
  • Y. Muramoto
    • 1
  • Y. Suzuki
    • 1
  • K. Tamura
    • 1
  1. 1.Department of Chemical Science and Technology, Faculty of EngineeringThe University of TokushimaTokushimaJapan

Personalised recommendations