Abstract
Solubility and growth rate of orthorhombic lysozyme (from hen egg-white) crystals were measured at 0.1 and 100 MPa. A growth or dissolution rate of a given concentration was measured, and the solubility was determined as the concentration at which the crystal did not grow or dissolve. The solubility decreased with increasing pressure at 30, 35 and 40 °C. This means that the supersaturation, σ (= lnC/C e, C: protein concentration, C e: solubility), increases; hence, the crystallization is enhanced with increasing pressure. The growth rates of (010) face, R(010), were replotted with σ, and the kinetic growth coefficients, k (= R(010) /σ), were estimated. The coefficient increased with increasing pressure at 30, 35 and 40 °C. This means that the growth kinetics is also enhanced_with increasing pressure.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Visuri, K., Kaipainen, E., Kivimaki, J, Niemi, H., Leisola, M. and Palosaari, S. (1990); Bio/Technology, 8, 547–549.
Gross, M. and Jaenicke, R. (1991); FEBS Lett., 284, 87–90.
Gross, M. and Jaenicke, R. (1993); Biophys. Chem., 45, 245–252.
Schall, C.A., Wiencek, J.M., Yarmush, M. and Arnold, E. (1994); J. Cryst. Growth, 135, 548–554.
Suzuki, Y., Miyashita, S., Komatsu, H., Sato, K. and Yagi, T. (1994); Jpn. J. Appl. Phys., 33, L1568–L1570.
Saikumar, M.V., Glatz, C.E. and Larson, M.A. (1995); J. Cryst. Growth, 151, 173–179.
Lorber, B., Jenner, G. and Giege, R. (1996); J. Cryst. Growth, 158, 103–117.
Takano, K.J., Harigae, H., Kawamura, Y. and Ataka, M. (1997); J. Cryst. Growth, 171, 554–558.
Saikumar, M.V., Glatz, C.E. and Larson, M.A. (1998); J. Cryst. Growth, 187, 277–288.
Sazaki, G., Nagatoshi, Y., Suzuki, Y., Durbin, S.D., Miyashita, S., Nakada, T. and Komatsu, H. (1999); J. Cryst. Growth, 196, 204–209.
Webb, J.N., Waghmare, R.Y., Carpenter, J.F., Glatz, C.E. and Randolph, T.W. (1999); J. Cryst. Growth, 205, 563–574.
Suzuki, Y., Miyashita, S., Sazaki, G., Nakada, T., Sawada, T. and Komatsu, H. (2000); J. Cryst. Growth, 208, 638–644.
Wagmare, R.Y., Webb, J.N., Randolph, T.W., Larson, M.A. and Glatz, C.E. (2000); J. Cryst. Growth, 208, 678–686.
Suzuki, Y., Sawada, T., Miyashita, S., Komatsu, H., Sazaki, G. and Nakada, T. (2000); J. Cryst. Growth, 209, 1018–1022.
Logtenberg, E.H.P., Meersman, F., Rubens, P., Heremans, K. and Frank, J. (2000); High Pressure Res., 19, 675–680.
Waghmare, R.Y., Pan, X.J. and Glatz, C.E. (2000); J. Cryst. Growth, 210, 746–752.
Suzuki, Y., Sazaki, G., Miyashita, S., Sawada, T., Tamura and K., Komatsu, H. (2002); Biochim. Biophys. Acta, 1595, 345–356.
Pan, X. and Glatz, C.E. (2002); Cryst. Growth and Design, 2, 45–50.
Suzuki, Y., Sazaki, G., Visuri, K., Tamura, K., Nakajima, K. and Shin-ichiro, Y. (2002); Cryst. Growth and Design, 2, 321–324.
For a discussion, see for example, Rosenberger, F., Vekilov, P.G., Muschol, M. and Thomas, B.R. (1996); J. Cryst. Growth, 168, 1–27.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Suzuki, Y., Suto, H., Sawada, T., Tamura, K. (2003). Effects of Pressure on the Growth Kinetics of Orthorhombic Lysozyme Crystals. In: Winter, R. (eds) Advances in High Pressure Bioscience and Biotechnology II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05613-4_30
Download citation
DOI: https://doi.org/10.1007/978-3-662-05613-4_30
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-05674-1
Online ISBN: 978-3-662-05613-4
eBook Packages: Springer Book Archive