Abstract
The temperature and pressure dependence on the secondary structure of α-chymotrypsin was studied by FT-IR spectroscopy. The temperature dependence of the secondary structures of α-chymotrypsin indicates that the temperature-induced unfolding of α-chymotrypsin sets in at 41°C and pD 7.0 at ambient pressure. Upon unfolding, the content of intermolecular β-sheet structures increases drastically, whereas that of intramolecular β-sheets decrease concomitantly. At room temperature (21°C), this protein denatures at ~4.9 kbar. No aggregation occurs in the pressure-denaturated state. A tentative p-T stability diagram was obtained on the basis of pressure dependent FT-IR measurements at different temperatures.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Battoste, E., Attanasio, F. and Rialdi, G. J. Therm. Anal. Cal., 61, 513–525 (2000).
Zhang, J., Peng, X., Jonas, A. and Jonas, J. Biochemistry 34, 8361–8641 (1995).
Mombelli, E., Afshar, M., Fusi, P., Mariani, M., Tortora, P., Connelly, J. P. and Lange, R. Biochemistry 36, 8733–8742 (1997).
Privalov, P. L. Crit. Rev. Biochem. Molec. Biol., 25, 281–305 (1990).
Panick, G., Malessa, R., Winter, R., Rapp, G., Frye, K. J. and Royer, C. A. J. Mol. Biol. 275, 389–402 (1998).
Prestrelski, S. J., Byler, D. M., and Liebmann, M. N., Biochemistry 30, 133–143 (1991).
Ismail, A. A., Mantsch, H. H., and Wong, P. T. T., Biochim. Biophys. Acta 1121, 183–188 (1992).
Qinglong, C., Huizhou, L., and Jiayong, C., Biochim. Biophys. Acta 1206, 247–252 (1994).
Hawley, S. A., Biochemistry 10, 2436–2442 (1971).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Sun, Z., Winter, R. (2003). Temperature- and Pressure-induced Unfolding of α-Chymotrypsin. In: Winter, R. (eds) Advances in High Pressure Bioscience and Biotechnology II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05613-4_21
Download citation
DOI: https://doi.org/10.1007/978-3-662-05613-4_21
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-05674-1
Online ISBN: 978-3-662-05613-4
eBook Packages: Springer Book Archive