Skip to main content
SpringerLink
Log in

Folding and Unfolding Behavior of Carboxypeptidase Y Inhibitor (IC) Induced by Temperature and Pressure

  • Conference paper
Advances in High Pressure Bioscience and Biotechnology II

  • 430 Accesses

Abstract

Carboxypeptidase Y (CPY) inhibitor, IC, is a heat-stable proteinase inhibitor produced by the yeast Saccharomyces cerevisiae [Matern, H., Hoffmann, M., and Holzer, H. (1974) Proc. Nat. Acad. Sci. USA 71, 4874–4878]. The heat- and pressure-induced unfolding of IC was investigated by means of fluorescence and circular dichroism spectroscopy, in order to better understand the structural basis for its high heat-stability. The heat-induced denaturation showed a two-state transition and was irreversible, while the pressure-induced denaturation showed a multiple-state transition and was completely reversible. The β-sheet of IC was converted into α-helix and random coil structures after heat treatment at 75 ºC whereas the secondary structure content remained unchanged after a pressure treatment at 400 MPa. Thus, it appears that the β-sheet-rich structure of IC is important for its structural stability.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Betz, H., Hinze, H., and Holzer, H. (1974) J. Biol. Chem. 249, 4515–4521.

    CAS  Google Scholar 

  2. Matern, H., Hoffmann, M., and Holzer, H. (1974) Proc. Nat. Acad. Sci. USA 71, 4874–4878.

    Article  CAS  Google Scholar 

  3. Matern, H., Barth, R., and Holzer, H. (1979) Biochim. Biophys. Acta 567, 503–510.

    Article  CAS  Google Scholar 

  4. Bruun, A.W., Svendsen, I., Sørensen, S.O., Kielland-Brandt, M.C., and Winther, J.R. (1998) Biochemistry 37, 3351–3357.

    Article  CAS  Google Scholar 

  5. Hayashi, R. (1976) Methods Enzymol. 45, 568–572.

    Article  CAS  Google Scholar 

  6. Silva, J., Miles, E., and Weber, G. (1986) Biochemistry 25, 5780–5786.

    Article  CAS  Google Scholar 

  7. Greenfield, N., and Fasman, G.D. (1969) Biochemistry 8, 4108–4116.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Joji Mima, Yo Sato, Michiko Kato & Rikimaru Hayashi

Authors
  1. Joji Mima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yo Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michiko Kato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rikimaru Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Chemistry, Physical Chemistry I, University of Dortmund, Otto-Hahn-Str. 6, 44227, Dortmund, Germany

    Roland Winter

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Mima, J., Sato, Y., Kato, M., Hayashi, R. (2003). Folding and Unfolding Behavior of Carboxypeptidase Y Inhibitor (IC) Induced by Temperature and Pressure. In: Winter, R. (eds) Advances in High Pressure Bioscience and Biotechnology II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05613-4_18

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-05613-4_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05674-1

  • Online ISBN: 978-3-662-05613-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation