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Adenovirus DNA Replication: Protein Priming, Jumping Back and the Role of the DNA Binding Protein DBP

  • Chapter
Adenoviruses: Model and Vectors in Virus-Host Interactions

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 272))

Abstract

The adenovirus (Ad) genome is a linear double-stranded (ds) molecule containing about 36 kilobase pairs. At each end of the genome an approximately 100 base pair (bp) inverted terminal repeat (ITR) is found, the exact length depending on the serotype. To the 5’-end of each ITR, a 55-kDa terminal protein (TP) is covalently coupled. The Ad DNA replication system was one of the first replication systems that could be reconstituted in vitro (Challberg and Kelly 1979). The system requires three virally encoded proteins: precursor TP (pTP), DNA polymerase (Pol) and the DNA binding protein (DBP). In addition, three stimulating human cellular proteins have been identified. These are the transcription factors NFI (Nagata et al. 1982) and Oct-1 (Pruijn et al. 1986) and the type I topoisomerase NFII (Nagata et al. 1983). Ad DNA replication uses a protein primer for replication initiation. The transition from initiation to elongation is marked by a jumping back mechanism (King and van der Vliet 1994), followed by elongation. In order to elongate DBP is required. In this review we discuss the roles of DBP during initiation and elongation and we relate biochemical data on the jumping back mechanism used by Ad Pol to the recently solved crystal structure of a Pol α-like replication complex (Franklin et al. 2001). We comment on the conditions and possible functions of jumping back and propose a model to describe the jumping back mechanism.

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Authors and Affiliations

  1. Department of Physiological Chemistry and Center for Biomedical Genetics, University Medical Center, Utrecht, The Netherlands

    R. N. de Jong, P. C. van der Vliet & A. B. Brenkman

  2. Universiteitsweg 100, P.O. Box 85060, 3508 AB, Utrecht, The Netherlands

    P. C. van der Vliet

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  1. R. N. de Jong
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  2. P. C. van der Vliet
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  3. A. B. Brenkman
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Editors and Affiliations

  1. Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, 91054, Erlangen, Germany

    Walter Doerfler

  2. Institut für Genetik, Universität zu Köln, Weyertal 121, 50931, Köln, Germany

    Petra Böhm

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© 2003 Springer-Verlag Berlin Heidelberg

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de Jong, R.N., van der Vliet, P.C., Brenkman, A.B. (2003). Adenovirus DNA Replication: Protein Priming, Jumping Back and the Role of the DNA Binding Protein DBP. In: Doerfler, W., Böhm, P. (eds) Adenoviruses: Model and Vectors in Virus-Host Interactions. Current Topics in Microbiology and Immunology, vol 272. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05597-7_7

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  • DOI: https://doi.org/10.1007/978-3-662-05597-7_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05517-1

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