Functional Expression of CXCR4 in S. cerevisiae: Development of Tools for Mechanistic and Pharmacologic Studies
Chemokines are cytokines that program directed migration of leukocyte subsets (Loetscher et al. 2000). These small proteins (~8–14 kDa) form the largest family of secreted intercellular messengers, having over 43 members recognized at this time. The family is characterized by the presence of four positionally conserved cysteine residues and subdivided into four branches based on the relationship of the two amino-proximal cysteines: C-C, C-X-C, C-X3-C, and C. Cellular signals for chemokines are transduced by members of the G-protein coupled receptor (GPCR) family that also cosegregate along these four subdivisions (Murphy et al. 2000). It is now recognized that in addition to promoting inflammation through recruitment of leukocyte subsets, chemokines play a central role in directing the development of tissues outside the hemato-lymphoid system.
KeywordsYeast Strain Reporter Gene Expression CXCR4 Antagonist Tyrosine Sulfation Pheromone Response Pathway
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