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Noninvasive Electrical Sensor Devices to Monitor Living Cells Online

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Ultrathin Electrochemical Chemo- and Biosensors

Part of the book series: Springer Series on Chemical Sensors and Biosensors ((SSSENSORS,volume 2))

Abstract

Experiments that rely on isolated animal cells in vitro are indispensable in all branches of biomedical research, especially in those areas addressing molecular interactions like drug discovery or molecular medicine. From the analytical viewpoint, these scientific goals not only require assays and devices capable of monitoring the interaction between two molecular species, but also to detect whether any biological functionality is generated. Accordingly, assays based on living cells as the sensing elements are often required and advantageous. The present article describes two transducer devices capable of monitoring changes in the three-dimensional shape of living cells when these are exposed to chemical, biological, or physical stimuli. In both technologies, the cells are cultured on thin conducting gold electrodes. The readout is based on electrochemical impedance spectroscopy or the quartz crystal microbalance technique. Besides a condensed outline about the methodological backgrounds, this article presents some selected examples about their successful application as whole-cell biosensors.

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Authors
  1. Andreas Janshoff
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  2. Claudia Steinem
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  3. Joachim Wegener
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Editor information

Editors and Affiliations

  1. Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany

    Vladimir M. Mirsky

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Janshoff, A., Steinem, C., Wegener, J. (2004). Noninvasive Electrical Sensor Devices to Monitor Living Cells Online. In: Mirsky, V.M. (eds) Ultrathin Electrochemical Chemo- and Biosensors. Springer Series on Chemical Sensors and Biosensors, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05204-4_9

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  • DOI: https://doi.org/10.1007/978-3-662-05204-4_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05961-2

  • Online ISBN: 978-3-662-05204-4

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