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Antibodies for Biosensors

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Ultrathin Electrochemical Chemo- and Biosensors

Part of the book series: Springer Series on Chemical Sensors and Biosensors ((SSSENSORS,volume 2))

Abstract

Biosensor sensitivity and selectivity essentially depend on the properties of the biorecognition elements to be used for analyte binding. Immunosensors employing antibodies as binding protefìts are effective tools for the analysis of a wide variety of analytes from the group of antigens and haptens. Genetic engineering provides an elegant way not only for the generation of virtually unlimited amounts of biorecognition molecules, but also for the alteration of existing properties and supplementation with additional functions. Different strategies for the synthesis of antibody fragment libraries, followed by the selection of specific antibody variants, were examined An antibody library was derived from a set of B cells. Chain shuffling of the antibody heavy and light chains provided the improved binders. An ELISA was achieved for the herbicide atrazine with an IC50 of 0.9 µg/1 and a detection limit of 0.2 µg/1, which corresponds to an equilibrium dissociation constant of 7.46×10−10 M as determined by a surface plasmon resonance-based biosensor. The close relations between the optimization of recombinant antibodies by evolutionary strategies and genetic algorithms are considered.

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References

  1. Scheller F, Schubert F (eds) (1989) Biosensoren. Akademie, Berlin

    Google Scholar 

  2. Canh TM (1993) Biosensors. Chapman & Hall, London

    Google Scholar 

  3. Spichiger-Keller UE (1998) Chemical sensors and biosensors for medical and biological applications. Wiley-VCH, Weinheim

    Book  Google Scholar 

  4. Hock B, Barceló D, Cammann K, Hansen P-D, Turner APF (eds) (1998) Biosensors for environmental diagnostics. Teubner, Stuttgart

    Google Scholar 

  5. Bilitewski U, Turner APF (eds) (2000) Biosensors for environmental monitoring. Harwood, Amsterdam

    Google Scholar 

  6. Hörsch S (1996) Diploma thesis, University of Stuttgart

    Google Scholar 

  7. Poljak RI, Amzel LM, Avey HP, Chen BL, Phizackerley RP, Saul F (1973) Proc Natl Acad Sci USA 70: 3305

    Article  CAS  Google Scholar 

  8. Wu TT, Kabat EA (1970) J Exp Med 132: 211

    Article  CAS  Google Scholar 

  9. Kabat EA, Wu TT, Perry HM, Gottesman KS, Foeller C (1991) Sequences of proteins of immunological interest, 5th edn. US Department of Health and Human Services, Public Health Service, National Institutes of Health (NIH Publication No 91–3242), Washington

    Google Scholar 

  10. Padlan EA (1994) Mol Immunol 31: 169

    Article  CAS  Google Scholar 

  11. Tulip WR, Varghese JN, Laver WG, Webster RG, Colman PM (1992) J Mol Biol 227: 122

    Article  CAS  Google Scholar 

  12. Van Oss CJ (1992) Antibody-antigen intermolecular forces. In: Roitt IM, Daves PJ (eds) Encyclopedia of immunology, vol 1. Academic, London, p 97

    Google Scholar 

  13. Köhler G, Milstein C (1975) Nature 256: 495

    Article  Google Scholar 

  14. Cooper MA (2002) Nat Rev Drug Discov 1: 515

    Article  CAS  Google Scholar 

  15. Kauffmann JM (2002) Ann Pharm Fr 60: 28

    CAS  Google Scholar 

  16. Raman SC, Raje M, Varshney GC (2002) Crit Rev Biotechnol 22: 15

    Article  Google Scholar 

  17. Rogers KR (2000) Mol Biotechnol 14: 109

    Article  CAS  Google Scholar 

  18. Better M, Chang CP, Robinson RR, Horwitz AH (1988) Science 240: 1041

    Article  CAS  Google Scholar 

  19. Skerra A, Plückthun A (1988) Science 240: 1038

    Article  CAS  Google Scholar 

  20. Huse WD, Sashy L, Iverson SA, Kang AS, Alting-Mees H, Burton DR, Benkovic JS, Lerner RA (1989) Science 246: 1275

    Article  CAS  Google Scholar 

  21. McCafferty J, Griffiths AD, Winter G, Chiswell DJ (1990) Nature 348: 552

    Article  CAS  Google Scholar 

  22. Ward VK, Schneider PG, Kreissig SB, Hammock BD, Choudary PV (1993) Protein Eng 6: 981

    Article  CAS  Google Scholar 

  23. Kramer K, Hock B (1996) Food Agric Immunol 8: 97

    Article  CAS  Google Scholar 

  24. Lee N, Holtzapple CK, Stanker LH (1998) J Agric Food Chem 46: 3381

    Article  CAS  Google Scholar 

  25. Li Y, Cockburn W, Kilpatrick J, Whitelam GC (1999) Food Agric Immunol 11: 5

    Article  CAS  Google Scholar 

  26. Boder ET, Midelfort KS, Wittrup KD (2000) Proc Natl Acad Sci USA 97: 10701

    Article  CAS  Google Scholar 

  27. Daugherty PS, Chen G, Iverson BL, Georgiou G (2000) Proc Natl Acad Sci USA 97: 2029

    Article  CAS  Google Scholar 

  28. Hanes J, Schaffitzel C, Knappik A, Plückthun A (2000) Nature Biotechnol 18: 1287

    Article  CAS  Google Scholar 

  29. Rajewsky K (1996) Nature 381: 751

    Article  CAS  Google Scholar 

  30. Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL, Curtiss L (1997) Nature Biotechnol 15: 29

    Article  CAS  Google Scholar 

  31. Boder ET, Wittrup KD (1997) Nature Biotechnol 15: 553

    Article  CAS  Google Scholar 

  32. Smith GP, Scott JK (1993) Meth Enzymol 217: 228

    Article  CAS  Google Scholar 

  33. Hanes J, Plückthun A (1997) Proc Natl Acad Sci USA 94: 4937

    Article  CAS  Google Scholar 

  34. Kramer K, Giersch T, Hock B (1994) Food Agric Immunol 6: 5

    Article  CAS  Google Scholar 

  35. Johnson B, Löfas S, Lindquist G (1991) Anal Biochem 198: 268

    Article  Google Scholar 

  36. Kazemier B, de Haard H, Boender P, van Gemen B, Hoogenboom H (1996) J Immunol Meth 194: 201

    Article  CAS  Google Scholar 

  37. K. Kramer, B. Hock

    Google Scholar 

  38. Kipriyanov SM, Little M (1999) Mol Biotechnol 12: 173

    Article  CAS  Google Scholar 

  39. Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR (1994) Ann Rev Immunol 12: 433

    Article  CAS  Google Scholar 

  40. Holland J (1975) Adaption in natural and artificial systems. University of Michigan Press, Ann Arbor

    Google Scholar 

  41. Goldberg DE (1989) Genetic algorithms in search, optimization, and learning. Addison-Wesley, Boston

    Google Scholar 

  42. Irving R, Hudson P (1995). Superseding hybridoma technology with phage display libraries. In: Zola H (ed) Monoclonal antibodies. The second generation. Bios, Oxford, P 119

    Google Scholar 

  43. Huber A, Demartis S, Neri D (1999) J Mol Recognit 12: 198

    Article  CAS  Google Scholar 

  44. Van Regenmortel MH, Altschuh D, Chatelier J, Rauffer Bruyere N, Richalet Secordel P, Saunai H (1997) Immunol Invest 26: 67

    Article  Google Scholar 

  45. Luppa PB, Sokoll LJ, Chan DW (2001) Clin Chim Acta 314: 1

    Article  CAS  Google Scholar 

  46. Wittmann C, Schmid RD (1994) J Agric Food Chem 42: 1041

    Article  CAS  Google Scholar 

  47. Ruzicka J, Hansen EH (1975) Chim Acta 78: 145

    Article  CAS  Google Scholar 

  48. Krämer PM (1998) Food Technol Biotechnol 36: 111

    Google Scholar 

  49. Minnuni M, Mascini M (1993) Anal Lett 26: 1441

    Article  Google Scholar 

  50. Ekins RP, Chu R, Biggart E (1990) Ann Biol Clin 48: 655

    CAS  Google Scholar 

  51. Brecht A, Klotz A, Barzen C, Gauglitz G, Harris RD, Quigley GR, Wilkinson JS, Sztajnbok P, Abuknesha R, Gascon J, Oubina A, Barcelo D (1998) Anal Chim Acta 362: 69

    Article  CAS  Google Scholar 

  52. Weller MG, Schuetz AJ, Winklmair M, Niessner R (1999) Anal Chim Acta 393: 29

    Article  CAS  Google Scholar 

  53. Kido H, Maquieira A, Hammock BD (2000) Anal Chim Acta 411: 1

    Article  CAS  Google Scholar 

  54. Decanniere K, Desmyter A, Lauwereys M, Ghahroudi MA, Muyldermans S, Wyns L (1999) Structure 7: 361

    Article  CAS  Google Scholar 

  55. Nutall SD, Rousch MJM, Irving RA, Hufton SE, Hoogenboom HR, Hudson PJ (1999) Proteins 36: 217

    Article  Google Scholar 

  56. Beste G, Schmidt FS, Stibora T, Skerra A (1999) Proc Natl Acad Sci USA 96: 1898

    Article  CAS  Google Scholar 

  57. Chen B, Bestetti G, Day RM, Turner APF (1998) Biosens Bioelectron 13: 779

    Article  CAS  Google Scholar 

  58. Haupt K, Mosbach K (2000) Chem Rev 100: 2495

    Article  CAS  Google Scholar 

  59. O’Sullivan CK (2002) Anal Bioanal Chem 372: 44

    Article  Google Scholar 

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Authors
  1. K. Kramer
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  2. B. Hock
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Editor information

Editors and Affiliations

  1. Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany

    Vladimir M. Mirsky

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© 2004 Springer-Verlag Berlin Heidelberg

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Kramer, K., Hock, B. (2004). Antibodies for Biosensors. In: Mirsky, V.M. (eds) Ultrathin Electrochemical Chemo- and Biosensors. Springer Series on Chemical Sensors and Biosensors, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05204-4_1

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  • DOI: https://doi.org/10.1007/978-3-662-05204-4_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05961-2

  • Online ISBN: 978-3-662-05204-4

  • eBook Packages: Springer Book Archive

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