DNA Microarray Analysis of Gene Expression Profiles in Hepatocellular Carcinoma
Differential gene expression profiles between normal tissue and Hepatocellular Carcinoma using DNA microarray are described. DNA microarrays were prepared by spotting PCR products of 4096 human genes onto specially treated glass slides. The cDNA probes were prepared by labeling normal tissue mRNA and cancer tissue mRNA with Cy3-dUTP and Cy5-dUTP separately through reverse transcription. The arrays were then hybridized against the cDNA probe mixture and the fluorescent signals were scanned. The data obtained from repeated experiments were analyzed. Around 1000 genes exhibit differentially expression profiles in hepatocellular carcinomas from several specimens, some of which have been proved to involve hepatocellular carcinogenesis. Northern blot was used to verify the array hybridization data. Some unreported genes are undergoing further research concerning their function. This technology provides a powerful method to elucidate tumor-specific gene expression profiles in human cancer.
KeywordsPhenol Leukemia Glutathione Chloroform Aldehyde
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